公开(公告)号：US11684918B2

公开(公告)日：2023-06-27

申请号：US16697845

申请日：2019-11-27

Applicant: IntegenX Inc.

Inventor： William D. Nielsen ,  Richard J. Belcinski ,  Gregory Bogdan ,  David Eberhart ,  Omar El-Sissi ,  Stevan B. Jovanovich ,  Michael Recknor ,  Ezra Van Gelder ,  David Wyrick

IPC: B01L3/00 ,  B01L7/00 ,  G01N27/447 ,  B01F33/30 ,  B01F33/452 ,  G01N35/00

CPC classification number: B01L3/502715 ,  B01F33/30 ,  B01F33/452 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  G01N27/44743 ,  G01N27/44791 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/043 ,  B01L2400/0421 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0616 ,  B01L2400/0638 ,  G01N2035/0091 ,  G01N2035/00158

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

公开(公告)号：US09663819B2

公开(公告)日：2017-05-30

申请号：US14552389

申请日：2014-11-24

Applicant: IntegenX Inc.

Inventor： Stevan B. Jovanovich ,  William D. Nielsen ,  Mattias Vangbo ,  Ezra Van Gelder ,  Omar El-Sissi

IPC: G01N1/10 ,  G01N33/00 ,  C12Q1/68 ,  G01N35/00 ,  G01N1/31 ,  G01N27/74 ,  G01N21/64 ,  G01N27/447 ,  G01N1/40 ,  G01J3/44

CPC classification number: C12Q1/6806 ,  G01J3/4406 ,  G01N1/31 ,  G01N1/405 ,  G01N21/64 ,  G01N27/44721 ,  G01N27/44791 ,  G01N27/745 ,  G01N35/00029 ,  G01N2035/00148 ,  G01N2035/00247 ,  G01N2201/06113 ,  G01N2201/062 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

公开(公告)号：US20150136602A1

公开(公告)日：2015-05-21

申请号：US14552389

申请日：2014-11-24

Applicant: INTEGENX INC.

Inventor： Stevan B. Jovanovich ,  William D. Nielsen ,  David S. Cohen ,  Michael Recknor ,  Mattias Vangbo ,  Ezra Van Gelder ,  Lars Majlof ,  Omar El-Sissi

IPC: C12Q1/68 ,  G01N21/64 ,  G01N27/447

CPC classification number: C12Q1/6806 ,  G01J3/4406 ,  G01N1/31 ,  G01N1/405 ,  G01N21/64 ,  G01N27/44721 ,  G01N27/44791 ,  G01N27/745 ,  G01N35/00029 ,  G01N2035/00148 ,  G01N2035/00247 ,  G01N2201/06113 ,  G01N2201/062 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

Abstract translation: 本发明提供一种可以处理原始生物样品，进行生化反应并提供分析读数的系统。 例如，该系统可以从拭子中提取DNA，从DNA扩增STR基因座，并分析样品中扩增的位点和STR标记。 该系统通过使用微流体组件来集成这些功能，以连接什么是宏流动功能。 在一个实施方案中，该系统包括样品纯化模块，反应模块，反应后净化模块，毛细管电泳模块和计算机。 在某些实施例中，该系统包括用于执行分析物捕获的一次性盒。 墨盒可以包括流体歧管，该流体歧管具有与微流体芯片配合的大流动腔室，所述微流体芯片在腔室之间引导液体。 该系统适合不超过10英尺3的外壳。 并且可以是封闭的，便携式的和/或电池供电的系统。 该系统可用于在不到4小时内从原始样品进行分析。

公开(公告)号：US09012236B2

公开(公告)日：2015-04-21

申请号：US13967957

申请日：2013-08-15

Applicant: IntegenX Inc.

Inventor： Stevan B. Jovanovich ,  William D. Nielsen ,  David S. Cohen ,  Michael Recknor ,  Mattias Vangbo ,  Ezra Van Gelder ,  Omar El-Sissi

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  C12Q1/68 ,  G01N35/00 ,  G01N1/31 ,  G01N27/74 ,  G01N1/40

CPC classification number: C12Q1/6806 ,  G01J3/4406 ,  G01N1/31 ,  G01N1/405 ,  G01N21/64 ,  G01N27/44721 ,  G01N27/44791 ,  G01N27/745 ,  G01N35/00029 ,  G01N2035/00148 ,  G01N2035/00247 ,  G01N2201/06113 ,  G01N2201/062 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

Abstract translation: 本发明提供一种可以处理原始生物样品，进行生化反应并提供分析读数的系统。 例如，该系统可以从拭子中提取DNA，从DNA扩增STR基因座，并分析样品中扩增的位点和STR标记。 该系统通过使用微流体组件来集成这些功能，以连接什么是宏流动功能。 在一个实施方案中，该系统包括样品纯化模块，反应模块，反应后净化模块，毛细管电泳模块和计算机。 在某些实施例中，该系统包括用于执行分析物捕获的一次性盒。 墨盒可以包括流体歧管，该流体歧管具有与微流体芯片配合的大流动腔室，所述微流体芯片在腔室之间引导液体。 该系统适合不超过10英尺3的外壳。 并且可以是封闭的，便携式的和/或电池供电的系统。 该系统可用于在不到4小时内从原始样品进行分析。

公开(公告)号：US20150024436A1

公开(公告)日：2015-01-22

申请号：US14381179

申请日：2013-02-28

Applicant: INTEGENX INC.

Inventor： David Eberhart ,  Yuan Li ,  James Ogg ,  Ezra Van Gelder ,  Stephen J. Williams ,  Timothy Woudenberg ,  Dean S. Burgi ,  William D. Nielsen

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  G01N27/447

CPC classification number: C12Q1/686 ,  B01L3/502715 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0616 ,  G01N27/44704 ,  G01N2035/00158 ,  C12Q2565/629

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

Abstract translation: 本公开提供了一种综合和自动化的样本回答系统，其从包含生物材料的样品开始，在少于两小时内产生遗传图谱。 在某些实施方案中，生物材料是DNA，并且遗传图谱涉及确定受试者的一个或多个位点（例如，遗传基因座）等位基因，例如STR（短串联重复）分布，例如如 CODIS系统 该系统可以执行若干操作，包括（a）提取和分离核酸; （b）在选择的位点（例如遗传基因座）扩增核苷酸序列; 和（c）扩增产物的检测和分析。 这些操作可以在包括多个集成模块的系统中进行，包括分析物准备模块; 检测和分析模块和控制模块。

公开(公告)号：US08562918B2

公开(公告)日：2013-10-22

申请号：US13717585

申请日：2012-12-17

Applicant: IntegenX Inc.

Inventor： Stevan B. Jovanovich ,  William D. Nielsen ,  David S. Cohen ,  Michael Recknor ,  Mattias Vangbo ,  Ezra Van Gelder ,  Lars Majlof ,  Omar El-Sissi

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC classification number: C12Q1/6806 ,  G01J3/4406 ,  G01N1/31 ,  G01N1/405 ,  G01N21/64 ,  G01N27/44721 ,  G01N27/44791 ,  G01N27/745 ,  G01N35/00029 ,  G01N2035/00148 ,  G01N2035/00247 ,  G01N2201/06113 ,  G01N2201/062 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

公开(公告)号：US20130224846A1

公开(公告)日：2013-08-29

申请号：US13717585

申请日：2012-12-17

Applicant: IntegenX Inc.

Inventor： Stevan B. Jovanovich ,  William D. Nielsen ,  David S. Cohen ,  Michael Recknor ,  Mattias Vangbo ,  Ezra Van Gelder ,  Lars Majlof ,  Omar El-Sissi

IPC: G01N1/31

CPC classification number: C12Q1/6806 ,  G01J3/4406 ,  G01N1/31 ,  G01N1/405 ,  G01N21/64 ,  G01N27/44721 ,  G01N27/44791 ,  G01N27/745 ,  G01N35/00029 ,  G01N2035/00148 ,  G01N2035/00247 ,  G01N2201/06113 ,  G01N2201/062 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

Abstract translation: 本发明提供一种可以处理原始生物样品，进行生化反应并提供分析读数的系统。 例如，该系统可以从拭子中提取DNA，从DNA扩增STR基因座，并分析样品中扩增的位点和STR标记。 该系统通过使用微流体组件来集成这些功能，以连接什么是宏流动功能。 在一个实施方案中，该系统包括样品纯化模块，反应模块，反应后净化模块，毛细管电泳模块和计算机。 在某些实施例中，该系统包括用于执行分析物捕获的一次性盒。 墨盒可以包括流体歧管，该流体歧管具有与微流体芯片配合的大流动腔室，所述微流体芯片在腔室之间引导液体。 该系统适合不超过10英尺3的外壳。 并且可以是封闭的，便携式的和/或电池供电的系统。 该系统可用于在不到4小时内从原始样品进行分析。