公开(公告)号：US10525467B2

公开(公告)日：2020-01-07

申请号：US15342914

申请日：2016-11-03

Applicant: IntegenX Inc.

Inventor： William D. Nielsen ,  Richard J. Belcinski ,  Gregory Bogdan ,  David Eberhart ,  Omar EL-Sissi ,  Stevan B. Jovanovich ,  Michael Recknor ,  Ezra Van Gelder ,  David W. Wyrick

IPC: B01L3/00 ,  B01L7/00 ,  G01N27/447 ,  B01F13/00 ,  B01F13/08 ,  G01N35/00

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

公开(公告)号：US08894946B2

公开(公告)日：2014-11-25

申请号：US13656503

申请日：2012-10-19

Applicant: IntegenX Inc.

Inventor： William D. Nielsen ,  Richard J. Belcinski ,  Gregory Bogdan ,  David Eberhart ,  Omar El-Sissi ,  Stevan B. Jovanovich ,  Michael Recknor ,  Ezra Van Gelder ,  David W. Wyrick

IPC: G01N21/75 ,  C07H21/00 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/68 ,  B01L3/502715 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0616 ,  G01N27/44704 ,  G01N2035/00158

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

Abstract translation: 本公开提供了一种综合和自动化的样本回答系统，其从包含生物材料的样品开始，在少于两小时内产生遗传图谱。 在某些实施方案中，生物材料是DNA，并且遗传图谱涉及确定受试者的一个或多个位点（例如，遗传基因座）等位基因，例如STR（短串联重复）分布，例如如 CODIS系统。 该系统可以执行多个操作，包括（a）提取和分离核酸; （b）在选择的位点（例如遗传基因座）扩增核苷酸序列; 和（c）扩增产物的检测和分析。 这些操作可以在包括多个集成模块的系统中进行，包括分析物准备模块; 检测和分析模块和控制模块。

公开(公告)号：US20230405589A1

公开(公告)日：2023-12-21

申请号：US18336352

申请日：2023-09-11

Applicant: IntegenX, Inc.

Inventor： William D. Nielsen ,  Richard J. Belcinski ,  Gregory Bogdan ,  David Eberhart ,  Omar El-Sissi ,  Stevan B. Jovanovich ,  Michael Recknor ,  Ezra Van Gelder ,  David W. Wyrick

IPC: B01L3/00 ,  G01N27/447 ,  B01L7/00 ,  B01F33/30 ,  B01F33/452

CPC classification number: B01L3/502715 ,  G01N27/44743 ,  G01N27/44791 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  B01F33/30 ,  B01F33/452 ,  B01L2400/0421 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/043 ,  B01L2400/0478 ,  G01N2035/0091

Abstract: A system for controlling custody of a sample can include a cartridge comprising a sample receptacle configured to receive an article configured to deliver a sample to the cartridge, a cartridge module having a cartridge receptacle configured to receive the cartridge; a housing enclosing the cartridge module and the cartridge. The housing can include a closable door; at least one sensor configured to produce a signal indicating: insertion or removal of an article into or from the sample receptacle; whether the cartridge is inserted into the cartridge receptacle; or whether the door is open or closed. The system can further include a computer programmed to record a signal from the at least one sensor for a recording period of time and to display a signal history to a user.

公开(公告)号：US11684918B2

公开(公告)日：2023-06-27

申请号：US16697845

申请日：2019-11-27

Applicant: IntegenX Inc.

Inventor： William D. Nielsen ,  Richard J. Belcinski ,  Gregory Bogdan ,  David Eberhart ,  Omar El-Sissi ,  Stevan B. Jovanovich ,  Michael Recknor ,  Ezra Van Gelder ,  David Wyrick

IPC: B01L3/00 ,  B01L7/00 ,  G01N27/447 ,  B01F33/30 ,  B01F33/452 ,  G01N35/00

CPC classification number: B01L3/502715 ,  B01F33/30 ,  B01F33/452 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  G01N27/44743 ,  G01N27/44791 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/043 ,  B01L2400/0421 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0616 ,  B01L2400/0638 ,  G01N2035/0091 ,  G01N2035/00158

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

公开(公告)号：US20170197213A1

公开(公告)日：2017-07-13

申请号：US15342914

申请日：2016-11-03

Applicant: IntegenX Inc.

Inventor： William D. Nielsen ,  Richard J. Belcinski ,  Gregory Bogdan ,  David Eberhart ,  Omar EL-Sissi ,  Stevan B. Jovanovich ,  Michael Recknor ,  Ezra Van Gelder ,  David W. Wyrick

IPC: B01L3/00 ,  G01N27/447 ,  B01L7/00

CPC classification number: B01L3/502715 ,  B01F13/0059 ,  B01F13/0818 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0616 ,  B01L2400/0638 ,  G01N27/44743 ,  G01N27/44791 ,  G01N2035/00158 ,  G01N2035/0091

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

公开(公告)号：US20130115607A1

公开(公告)日：2013-05-09

申请号：US13656503

申请日：2012-10-19

Applicant: IntegenX Inc.

Inventor： William D. Nielsen ,  Richard J. Belcinski ,  Gregory Bogdan ,  David Eberhart ,  Omar El-Sissi ,  Stevan B. Jovanovich ,  Michael Recknor ,  Ezra Van Gelder ,  David W. Wyrick

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  B01L3/502715 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0616 ,  G01N27/44704 ,  G01N2035/00158

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

Abstract translation: 本公开提供了一种综合和自动化的样本回答系统，其从包含生物材料的样品开始，在少于两小时内产生遗传图谱。 在某些实施方案中，生物材料是DNA，并且遗传图谱涉及确定受试者的一个或多个位点（例如，遗传基因座）等位基因，例如STR（短串联重复）分布，例如如 CODIS系统。 该系统可以执行若干操作，包括（a）提取和分离核酸; （b）在选择的位点（例如遗传基因座）扩增核苷酸序列; 和（c）扩增产物的检测和分析。 这些操作可以在包括多个集成模块的系统中进行，包括分析物准备模块; 检测和分析模块和控制模块。