公开(公告)号：US11318459B2

公开(公告)日：2022-05-03

申请号：US14426555

申请日：2013-09-04

Applicant: Becton, Dickinson and Company

Inventor： Song Shi ,  Mei Yang ,  Klaus W. Berndt ,  Robert J. Losada ,  Jamieson W. Crawford ,  Ravi Attri ,  Christopher A. Battles ,  Benjamin R. Bartfeld ,  Gregory R. Hires

IPC: B01L3/00 ,  G01N1/40

Abstract: A method for detecting the presence of a microorganism in a fluid sample is disclosed. The method includes providing a fluid specimen within a specimen collection container having a sidewall defining an interior therein. The interior includes a mechanical separator adapted for separating the fluid sample into first and second phases within the specimen collection container and a sensing element capable of detecting the presence of a microorganism disposed therein. The method includes subjecting the specimen collection container to applied rotational force to isolate a concentrated microorganism region. The method also includes detecting by a sensing element the presence or absence of a microorganism within the concentrated microorganism region.

公开(公告)号：US20200149085A1

公开(公告)日：2020-05-14

申请号：US16734826

申请日：2020-01-06

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo

IPC: C12Q1/04 ,  G01N33/68 ,  G01N33/483

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

公开(公告)号：US10557162B2

公开(公告)日：2020-02-11

申请号：US15472759

申请日：2017-03-29

Applicant: Becton, Dickinson and Company

Inventor： Liping Feng ,  William B. Brasso ,  Susan M. Kircher ,  Vanda White ,  Song Shi ,  Xiao Mo ,  Tuan-Linh Ngoc Nguyen ,  Adrien P. Malick ,  Jon E. Salomon ,  John D. Mantlo

IPC: C12Q1/04 ,  G01N33/68 ,  G01N33/483 ,  H01J49/16 ,  H01J49/40

Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

公开(公告)号：US09970896B2

公开(公告)日：2018-05-15

申请号：US15602934

申请日：2017-05-23

Applicant: Becton, Dickinson and Company

Inventor： Song Shi ,  James G. Nadeau ,  Michael A. Brasch

IPC: G01N27/414 ,  B01L3/00 ,  C12Q1/04 ,  G01N33/487

CPC classification number: G01N27/4145 ,  B01L3/5021 ,  B01L2300/0636 ,  B01L2300/0851 ,  B01L2300/0893 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  C12Q1/04 ,  G01N33/48735 ,  G01N2800/26

Abstract: An array of micro-chambers (220) with ISFETs (300) disposed therein for monitoring single cell activity in the microarray to determine the presence or absence of microorganisms in a sample (390).

公开(公告)号：US09885084B2

公开(公告)日：2018-02-06

申请号：US15649098

申请日：2017-07-13

Applicant: Becton, Dickinson and Company

Inventor： Song Shi ,  Richard L. Moore ,  James Garrett

IPC: C12Q1/68 ,  G01N33/74 ,  G01N33/92

CPC classification number: C12Q1/6881 ,  C12Q2600/158 ,  G01N33/74 ,  G01N33/92 ,  G01N2333/90209 ,  G01N2333/91051 ,  G01N2800/26

Abstract: The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.

公开(公告)号：US20170261464A1

公开(公告)日：2017-09-14

申请号：US15602934

申请日：2017-05-23

Applicant: Becton, Dickinson and Company

Inventor： Song Shi ,  James G. Nadeau ,  Michael A. Brasch

IPC: G01N27/414 ,  G01N33/487 ,  C12Q1/04 ,  B01L3/00

CPC classification number: G01N27/4145 ,  B01L3/5021 ,  B01L2300/0636 ,  B01L2300/0851 ,  B01L2300/0893 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  C12Q1/04 ,  G01N33/48735 ,  G01N2800/26

Abstract: An array of micro-chambers (220) with ISFETs (300) disposed therein for monitoring single cell activity in the microarray to determine the presence or absence of microorganisms in a sample (390).

公开(公告)号：US20150118677A1

公开(公告)日：2015-04-30

申请号：US14390596

申请日：2013-04-04

Applicant: Becton, Dickinson and Company

Inventor： Xiao Li ,  Yongqiang Zhang ,  Kenneth Anthony Kopher ,  John D. Mantlo ,  William Alfred Pope ,  Song Shi ,  Axel A. Yup

IPC: C12Q1/06 ,  G01N1/30

CPC classification number: G01N1/30 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2001/302

Abstract: Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.

Abstract translation: 本文描述了用于鉴定和分析样品中的至少一种微生物（例如细菌）的方法和试剂，并且通过流式细胞术分析样品时降低了获得的背景信号强度。 通过将样品与背景信号降低分子或与猝灭剂共价连接的核酸染色组合来制备样品。 在用核酸染色剂染色之前，可以用树脂去除样品中的一部分颗粒物质。

公开(公告)号：US08603769B2

公开(公告)日：2013-12-10

申请号：US13647072

申请日：2012-10-08

Applicant: Becton, Dickinson and Company

Inventor： Liping Feng ,  William B. Brasso ,  Song Shi ,  Ben Turng ,  Susan M. Kircher ,  Vanda White ,  Dyan Luper ,  Julie Rosales ,  Gretta Campbell ,  Adrien Malick

IPC: C12Q1/04 ,  C12Q1/24

CPC classification number: C12Q1/24

Abstract: Methods of the invention include the isolation of intact, viable microorganism(s) from positive blood culture (“PBC”) samples for use in downstream analyses such as identification and antimicrobial susceptibility testing (“AST”). The methods involve collecting a portion of the PBC sample, adding a choline-containing solution, lysing the blood cells, isolating the viable microorganism, and performing downstream analysis of the isolated, viable microorganism. The methods can be applied to a variety of gram-positive bacteria, gram-negative bacteria, and/or yeast, and particularly to strains of S. pneumoniae.

Abstract translation: 本发明的方法包括从用于下游分析如鉴定和抗微生物敏感性测试（“AST”）的阳性血培养（“PBC”）样品中分离完整的活的微生物。 所述方法包括收集一部分PBC样品，加入含胆碱的溶液，裂解血细胞，分离活微生物，以及进行分离的活的微生物的下游分析。 该方法可以应用于各种革兰氏阳性细菌，革兰氏阴性细菌和/或酵母，特别是肺炎链球菌菌株。

公开(公告)号：US09645057B2

公开(公告)日：2017-05-09

申请号：US14774392

申请日：2013-04-04

Applicant: Becton, Dickinson and Company

Inventor： Xiao Li ,  Kenneth Anthony Kopher ,  John D. Mantlo ,  William Alfred Pope ,  Song Shi ,  Axel A. Yup

IPC: C12Q1/68 ,  G01N1/30 ,  C12Q1/06 ,  C12Q1/04

CPC classification number: G01N1/30 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2001/302

Abstract: A process for determining one of the presence, absence, or total of microorganisms (e.g. bacteria) in a sample. According to the process, a biological sample containing complex matrices is obtained. The sample is first combined with a resin to adsorb complex matrices from the sample. The resin is removed from the biological sample. The sample so prepared is then analyzed by flow cytometry.

公开(公告)号：US20160040233A1

公开(公告)日：2016-02-11

申请号：US14743735

申请日：2015-06-18

Applicant: Becton, Dickinson and Company

Inventor： Song Shi ,  Richard L. Moore ,  James Garrett

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q2600/158 ,  G01N33/74 ,  G01N33/92 ,  G01N2333/90209 ,  G01N2333/91051 ,  G01N2800/26

Abstract: The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.

Abstract translation: 本发明涉及可用于例如用于对象中败血症的高级检测的方法，监视器和系统。