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公开(公告)号：US20220170065A1
公开(公告)日：2022-06-02
申请号：US17521410
申请日：2021-11-08
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Liping Feng , William B. Brasso , Susan M. Kircher , Vanda White , Song Shi , Xiao Mo , Tuan-Linh Ngoc Nguyen , Adrien P. Malick , Jon E. Salomon , John D. Mantlo
IPC: C12Q1/04 , G01N33/68 , G01N33/483
Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
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公开(公告)号：US20200080919A1
公开(公告)日：2020-03-12
申请号：US16570006
申请日：2019-09-13
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Timothy Wiles , John D. Mantlo
Abstract: Methods for preparing a biological sample for testing by Maldi where such methods are selected based on sample parameters. Maldi scores are obtained for a range of sample parameters (e.g. McFarland, dispense volume and number of dispenses). From the data, sample preparation parameters can be selected for a biological sample being prepared for Maldi testing. One sample preparation strategy uses multiple dispenses of sample with an intervening drying step, which yields more accurate Maldi scores, particularly for samples at the low range of McFarland values (e.g. below about 2).
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公开(公告)号：US09933341B2
公开(公告)日：2018-04-03
申请号：US14390596
申请日：2013-04-04
Applicant: Becton, Dickinson and Company
Inventor： Xiao Li , Yongqiang Zhang , Kenneth Anthony Kopher , John D. Mantlo , William Alfred Pope , Song Shi , Axel A. Yup
CPC classification number: G01N1/30 , C12Q1/04 , C12Q1/06 , G01N2001/302
Abstract: Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.
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公开(公告)号：US11193158B2
公开(公告)日：2021-12-07
申请号：US16734826
申请日：2020-01-06
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Liping Feng , William B. Brasso , Susan M. Kircher , Vanda White , Song Shi , Xiao Mo , Tuan-Linh Ngoc Nguyen , Adrien P. Malick , Jon E. Salomon , John D. Mantlo
IPC: C12Q1/04 , G01N33/68 , G01N33/483 , H01J49/16 , H01J49/40
Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
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15.发明申请公开(公告)号：US20200087702A1
公开(公告)日：2020-03-19
申请号：US16677092
申请日：2019-11-07
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Susan M. Kircher , Vanda White , William B. Brasso , Dyan Luper , James Y. Zhou , Julie L. Rosales , Jeffery H. Bruton , John D. Mantlo , Adrien P. Malick , Donald R. Callihan , Ben Turng , Liping Feng , Curtis M. Gosnell , Patrick Shawn Beaty , John P. Douglass
Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.
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Patent Agency Ranking
16.发明申请公开(公告)号：US20180243735A1
公开(公告)日：2018-08-30
申请号：US15903767
申请日：2018-02-23
Applicant: BECTON DICKINSON AND COMPANY
Inventor： John D. Mantlo , Tong Zhou
CPC classification number: B01L3/021 , B01L3/0275 , B01L2300/0627 , B01L2300/069 , B01L2400/0487 , C12Q1/24 , G01N1/12 , G01N35/00584 , G01N35/0099 , G01N35/10 , G01N2001/028 , G01N2035/1027 , G01N2035/103
Abstract: The present disclosure describes pipette tips haying an absorbent material (e.g., a flocking material) anchored to a distal end of the pipette tip and related methods of use. In some embodiments, the flocked pipette tips can be used in an automated process and/or system, wherein the contact between the pipette tips and either a sample or a culture medium can be automatically sensed for accurate sample collection and/or dispense. In some embodiments, automatic detection of the liquid interface may be accomplished by detecting a threshold change in capacitance when the pipette tip contacts the sample liquid interface (e.g., for sample collection) or the agar interface (e.g., for sample release). In some embodiments, the automated process and/or system may utilize one or more predetermined, fixed heights for collecting samples and/or depositing samples.
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公开(公告)号：US20170204448A1
公开(公告)日：2017-07-20
申请号：US15472759
申请日：2017-03-29
Applicant: Becton, Dickinson and Company
Inventor： Liping Feng , William B. Brasso , Susan M. Kircher , Vanda White , Song Shi , Xiao Mo , Tuan-Linh Ngoc Nguyen , Adrien P. Malick , Jon E. Salomon , John D. Mantlo , Mary R. Votta , Ben Turng , Donald R. Callihan , Wendy Louise Williams
IPC: C12Q1/04 , G01N33/483
CPC classification number: C12Q1/04 , G01N33/4833 , G01N33/6851 , H01J49/164 , H01J49/40
Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
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18.发明授权公开(公告)号：US11225681B2
公开(公告)日：2022-01-18
申请号：US16677092
申请日：2019-11-07
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Susan M. Kircher , Vanda White , William B. Brasso , Dyan Luper , James Y. Zhou , Julie L. Rosales , Jeffery H. Bruton , John D. Mantlo , Adrien P. Malick , Donald R. Callihan , Ben Turng , Liping Feng , Curtis M. Gosnell , Patrick Shawn Beaty , John P. Douglass
Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.
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公开(公告)号：US20200149085A1
公开(公告)日：2020-05-14
申请号：US16734826
申请日：2020-01-06
Applicant: BECTON DICKINSON AND COMPANY
Inventor： Liping Feng , William B. Brasso , Susan M. Kircher , Vanda White , Song Shi , Xiao Mo , Tuan-Linh Ngoc Nguyen , Adrien P. Malick , Jon E. Salomon , John D. Mantlo
IPC: C12Q1/04 , G01N33/68 , G01N33/483
Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
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公开(公告)号：US10557162B2
公开(公告)日：2020-02-11
申请号：US15472759
申请日：2017-03-29
Applicant: Becton, Dickinson and Company
Inventor： Liping Feng , William B. Brasso , Susan M. Kircher , Vanda White , Song Shi , Xiao Mo , Tuan-Linh Ngoc Nguyen , Adrien P. Malick , Jon E. Salomon , John D. Mantlo
IPC: C12Q1/04 , G01N33/68 , G01N33/483 , H01J49/16 , H01J49/40
Abstract: Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.
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