知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

22 条记录 (耗时 0.032 秒)

    Methods using pores
    13.
    发明授权
    Methods using pores 有权
    使用孔的方法

    公开(公告)号：US08785211B2

    公开(公告)日：2014-07-22

    申请号：US13338794

    申请日：2011-12-28

    申请人： Hagan Bayley ,  Yann Astier ,  Orit Braha

    发明人： Hagan Bayley ,  Yann Astier ,  Orit Braha

    IPC分类号： C12Q1/68 ,  G01N33/543 ,  G01N27/26

    CPC分类号： G01N33/48721 ,  C12Q1/6869 ,  C12Q2565/631

    摘要： The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.

    摘要翻译： 本发明涉及鉴定个体核苷酸的方法，其包括（a）使核苷酸与跨膜蛋白质孔接触，使得核苷酸与孔相互作用，和（b）在相互作用期间测量通过孔的电流，从而确定 核苷酸的同一性。 本发明还涉及对与其相关的核酸序列和试剂盒进行测序的方法。

    METHODS USING PORES
    14.
    发明申请
    METHODS USING PORES 有权
    使用孔的方法

    公开(公告)号：US20120219960A1

    公开(公告)日：2012-08-30

    申请号：US13338794

    申请日：2011-12-28

    申请人： Hagan BAYLEY ,  Yann ASTIER ,  Orit BRAHA

    发明人： Hagan BAYLEY ,  Yann ASTIER ,  Orit BRAHA

    IPC分类号： G01N27/26

    CPC分类号： G01N33/48721 ,  C12Q1/6869 ,  C12Q2565/631

    摘要： The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.

    摘要翻译： 本发明涉及鉴定个体核苷酸的方法，其包括（a）使核苷酸与跨膜蛋白质孔接触，使得核苷酸与孔相互作用，和（b）在相互作用期间测量通过孔的电流，从而确定 核苷酸的同一性。 本发明还涉及对与其相关的核酸序列和试剂盒进行测序的方法。

    Nanopore sensor system
    16.
    发明申请
    Nanopore sensor system 审中-公开
    纳米孔传感器系统

    公开(公告)号：US20070298511A1

    公开(公告)日：2007-12-27

    申请号：US11788293

    申请日：2007-04-19

    申请人： Xiaofeng Kang ,  Stephen Cheley ,  Hagan Bayley

    发明人： Xiaofeng Kang ,  Stephen Cheley ,  Hagan Bayley

    IPC分类号： G01N27/00 ,  B01J13/02

    CPC分类号： G01N33/48721

    摘要： The invention relates to a nanopore sensor system including methods of fabrication and uses disclosed herein. In some embodiments, the invention relates to a substrate comprising a lipid membrane, preferably a phospholipid bilayer film, having a nanopore and a gel surrounding said lipid membrane. In additional embodiments, the invention relates to compositions and methods of using and making a substrate that has a lipid membrane having a single channel protein surrounded with a gel. In further embodiments, the invention relates to a method of detecting an analyte by mixing a nanopore sensor with a solution suspected of containing an analyte, measuring electrical properties, and correlating changes of electrical properties to the existence of an analyte.

    摘要翻译： 本发明涉及包括本文公开的制造方法和用途的纳米孔传感器系统。 在一些实施方案中，本发明涉及包含脂质膜，优选磷脂双层膜的底物，其具有纳米孔和围绕所述脂质膜的凝胶。 在另外的实施方案中，本发明涉及使用和制备具有由凝胶包围的单通道蛋白质的脂质膜的底物的组合物和方法。 在另外的实施方案中，本发明涉及一种通过将纳米孔传感器与疑似含有分析物的溶液混合来检测分析物的方法，测量电性能，以及将电特性的变化与分析物的存在相关联。

    High temperature ion channels and pores
    19.
    发明授权
    High temperature ion channels and pores 有权
    高温离子通道和孔

    公开(公告)号：US07867716B2

    公开(公告)日：2011-01-11

    申请号：US11314174

    申请日：2005-12-21

    申请人： Xiaofeng Kang ,  Li Qun Gu ,  Stephen Cheley ,  Hagan Bayley

    发明人： Xiaofeng Kang ,  Li Qun Gu ,  Stephen Cheley ,  Hagan Bayley

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54366 ,  G01N33/48721 ,  G01N33/54393 ,  G01N33/6872

    摘要： The present invention includes an apparatus, system and method for stochastic sensing of an analyte to a protein pore. The protein pore may be an engineer protein pore, such as an ion channel at temperatures above 55° C. and even as high as near 100° C. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable electrical current signal. Possible signals include change in electrical current. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may also be detected.

    摘要翻译： 本发明包括用于将分析物随机感测到蛋白质孔的装置，系统和方法。 蛋白质孔可以是工程蛋白孔，例如在高于55℃的温度下甚至高达100℃的离子通道。分析物可以是任何反应性分析物，包括化学武器，环境毒素和药物。 分析物共价键合到传感器元件以产生可检测的电流信号。 可能的信号包括电流变化。 信号的检测允许鉴定分析物并确定其在样品溶液中的浓度。 也可以检测存在于相同溶液中的多种分析物。

    Stochastic Sensing Through Covalent Interactions
    20.
    发明申请
    Stochastic Sensing Through Covalent Interactions 有权
    通过共价相互作用的随机感知

    公开(公告)号：US20090283422A1

    公开(公告)日：2009-11-19

    申请号：US12429897

    申请日：2009-04-24

    申请人： Hagan Bayley ,  Seong-Ho Shin ,  Tudor Luchian ,  Stephen Cheley

    发明人： Hagan Bayley ,  Seong-Ho Shin ,  Tudor Luchian ,  Stephen Cheley

    IPC分类号： G01N27/26

    CPC分类号： G01N33/5308 ,  C12Q1/001 ,  G01N33/5438 ,  G01N33/6872

    摘要： A system and method for stochastic sensing in which the analyte covalently bonds to the sensor element or an adaptor element. If such bonding is irreversible, the bond may be broken by a chemical reagent. The sensor element may be a protein, such as the engineered PSH type or αHL protein pore. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable signal. Possible signals include change in electrical current, change in force, and change in fluorescence. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may be detected.

    摘要翻译： 用于随机感测的系统和方法，其中分析物共价键合到传感器元件或适配器元件。 如果这种键合是不可逆的，则该键可能被化学试剂破坏。 传感器元件可以是蛋白质，例如工程化的PSH型或αHL蛋白质孔。 分析物可以是任何反应性分析物，包括化学武器，环境毒素和药物。 分析物共价键合到传感器元件以产生可检测的信号。 可能的信号包括电流的变化，力的变化和荧光的变化。 信号的检测允许鉴定分析物并确定其在样品溶液中的浓度。 可以检测存在于相同溶液中的多种分析物。