公开(公告)号：US09778112B2

公开(公告)日：2017-10-03

申请号：US15338851

申请日：2016-10-31

Applicant: University of Virginia Patent Foundation

Inventor： Brooks Hart Pate ,  Justin L. Neill

IPC: G01J3/30 ,  G01J3/443 ,  G01J3/28 ,  G01J3/433 ,  G01N21/3586 ,  G01N21/35

CPC classification number: G01J3/443 ,  G01J3/2889 ,  G01J3/4338 ,  G01J3/453 ,  G01N21/35 ,  G01N21/3586 ,  G01N2021/3595

Abstract: An emission can be obtained from a sample in response to excitation using a specified range of excitation frequencies. Such excitation can include generating a specified chirped waveform and a specified downconversion local oscillator (LO) frequency using a digital-to-analog converter (DAC), upconverting the chirped waveform via mixing the chirped waveform with a specified upconversion LO frequency, frequency multiplying the upconverted chirped waveform to provide a chirped excitation signal for exciting the sample, receiving an emission from sample, the emission elicited at least in part by the chirped excitation signal, and downconverting the received emission via mixing the received emission with a signal based on the specified downconversion LO signal to provide a downconverted emission signal within the bandwidth of an analog-to-digital converter (ADC). The specified chirped waveform can include a first chirped waveform during a first duration, and a second chirped waveform during a second duration.

公开(公告)号：US20160377547A1

公开(公告)日：2016-12-29

申请号：US15196354

申请日：2016-06-29

Applicant: Cedars-Sinai Medical Center

Inventor： Pramod Butte ,  Paul Lapchak ,  David Scott Kittle

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  A61B5/0071 ,  A61B5/0084 ,  A61B5/14556 ,  A61B5/1459 ,  A61B5/4866 ,  G01J3/0218 ,  G01J3/2889 ,  G01J3/4406 ,  G01N21/64 ,  G01N21/6402 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/6417 ,  G01N2021/6421 ,  G01N2021/6484 ,  G01N2201/06113

Abstract: The invention provides systems for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation and methods for using the same. The system includes a laser source, collection fibers, a demultiplexer and an optical delay device. All references cited herein are incorporated by reference in their entirety as though fully set forth. Unless defined otherwise, technical and scientific tens used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

Abstract translation: 本发明提供了通过在激发时分析来自生物样品的荧光的发射以及使用生物样品的方法来表征生物样品的系统。 该系统包括激光源，收集光纤，解复用器和光学延迟器件。 本文引用的所有参考文献通过引用整体并入，如同完全阐述一样。 除非另有定义，本文所用的技术和科学几何具有与本发明所属领域的普通技术人员通常理解的相同的含义。

公开(公告)号：US09383260B1

公开(公告)日：2016-07-05

申请号：US13492923

申请日：2012-06-10

Applicant: Jong Huyn Yoo ,  Randolph S. Tribe ,  Chunyi Liu

Inventor： Jong Huyn Yoo ,  Randolph S. Tribe ,  Chunyi Liu

IPC: G01J3/30 ,  G01J3/443

CPC classification number: G01J3/443 ,  B23K26/03 ,  B23K26/032 ,  B23K26/048 ,  B23K26/0861 ,  G01J3/0208 ,  G01J3/0218 ,  G01J3/0291 ,  G01J3/0294 ,  G01J3/2889 ,  G01N21/718 ,  H01J49/0463

Abstract: Systems, methods, compositions, and apparatus for laser induced ablation spectroscopy are disclosed. A sample site position sensor, stage position motors operable to move the stage in three independent spatial coordinate directions, and a stage position control circuit can move an analysis sample site to selected coordinate positions for laser ablation. Light emitted from a plasma plume produced with laser ablation can be gathered into a lightguide fiber bundle that is subdivided into branches. One branch can convey a first portion of the light to a broadband spectrometer operable to analyze a relatively wide spectral segment, and a different branch can convey a second portion of the light to a high dispersion spectrometer operable to measure minor concentrations and/or trace elements. Emissions from a plasma plume can be simultaneously analyzed in various ways using a plurality of spectrometers having distinct and/or complementary capabilities.

Abstract translation: 公开了用于激光诱导消融光谱的系统，方法，组合物和装置。 样本位置传感器，可操作以在三个独立的空间坐标方向上移动平台的平台位置传感器和平台位置控制电路可以将分析样本位置移动到用于激光消融的选定的坐标位置。 从激光烧蚀产生的等离子体羽流发出的光可以聚集成光纤纤维束，细分为分支。 一个分支可以将光的第一部分传送到可操作以分析相对宽的光谱段的宽带光谱仪，并且不同的分支可以将第二部分的光传送到高分散光谱仪，其可操作以测量较小的浓度和/或微量元素 。 可以使用具有不同和/或互补能力的多个光谱仪以各种方式同时分析来自等离子体羽流的排放。

公开(公告)号：US09377405B2

公开(公告)日：2016-06-28

申请号：US14348261

申请日：2012-09-20

Applicant: Carl Zeiss Microscopy GmbH

Inventor： Gunter Möhler ,  Dietmar Schmidt ,  Oliver Holub ,  Robert Hauschild

IPC: G01J1/58 ,  G01N21/64 ,  G01J3/28 ,  G02B21/00 ,  G01J1/44 ,  G01J3/44

CPC classification number: G01N21/64 ,  G01J1/44 ,  G01J3/2889 ,  G01J3/44 ,  G01N21/6408 ,  G01N21/6458 ,  G02B21/0076 ,  G02B21/0084

Abstract: An improved evaluation circuit which allows high sensitivity in an economical manner. For this purpose, a shift register having at least one data input, a clock input, a plurality of register stages and at least one data output is provided, wherein the output of the analog-to-digital converter is connected to the data input of the shift register. With a shift register, fluorescent light and scattered light can be temporally distinguished in an economical manner.

Abstract translation: 改进的评估电路，以经济的方式允许高灵敏度。 为此，提供具有至少一个数据输入，时钟输入，多个寄存器级和至少一个数据输出的移位寄存器，其中模数转换器的输出连接到 移位寄存器。 通过移位寄存器，可以以经济的方式在时间上区分荧光灯和散射光。

公开(公告)号：US20130218479A1

公开(公告)日：2013-08-22

申请号：US13848723

申请日：2013-03-21

Applicant: Ricardo J. Claps

Inventor： Ricardo J. Claps

IPC: G01N21/64 ,  G06F17/10

CPC classification number: G01N21/64 ,  G01J3/02 ,  G01J3/0205 ,  G01J3/0218 ,  G01J3/0256 ,  G01J3/10 ,  G01J3/2889 ,  G01J3/4406 ,  G01N21/6408 ,  G06F17/10

Abstract: A time-resolved spectroscopy system employing a time-division multiplexing optical device with no dispersive optical elements to perform lifetime and concentration measurements in multi-species samples, is disclosed. Some examples include fluorescence and cavity ring-down spectroscopy. The system is unique in its compactness and simplicity of operation. In one embodiment, the system makes use of only one photo-detector and an efficient linear regression algorithm. The system offers a measurement time for multiple species measurements of less than 1 s. The system can also be used to perform fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy. Four methods to de-convolve a multi-component, exponentially decaying optical signal such as obtained with the system disclosed here, are presented. These methods may be applied to the measurement of fluorescence decay lifetimes and cavity ring-down times, the latter used extensively for the measurement of gas and trace-gas concentrations in complex mixtures, via absorption spectroscopy.

Abstract translation: 公开了一种使用没有色散光学元件的时分复用光学器件来执行多品种样品中的寿命和浓度测量的时间分辨光谱系统。 一些实例包括荧光和空腔衰减光谱。 该系统具有独特的紧凑性和操作简单性。 在一个实施例中，系统仅使用一个光电检测器和有效的线性回归算法。 该系统为少于1秒的多种物种测量提供测量时间。 该系统还可用于进行荧光相关光谱和荧光互相关光谱。 提出了四种解卷积多分量指数衰减光信号的方法，例如用这里公开的系统获得的。 这些方法可以应用于荧光衰减寿命和空腔衰减时间的测量，后者广泛用于通过吸收光谱测量复杂混合物中的气体和痕量气体浓度。

公开(公告)号：US20130155412A1

公开(公告)日：2013-06-20

申请号：US13633569

申请日：2012-10-02

Applicant: Industrial Technology Research Institute

Inventor： Kuang-Po Chang ,  Shu-Yi Wang

IPC: G01J3/45 ,  G01J3/447

CPC classification number: G01J3/2889 ,  G01J3/0224 ,  G01J3/4406 ,  G01J3/443 ,  G01N21/718

Abstract: A spectrum detecting device including a laser apparatus, an optical splitting apparatus, an optical gate, a first polarizer, a second polarizer, and an optical analysis apparatus is provided. The optical splitting apparatus splits the laser beam providing from the laser apparatus into a first and a second light beam, and the second light beam is transmitted to a sample to produce a spectral signal. The optical gate activated by the first light beam is disposed between the optical analysis apparatus and the sample. The first polarizer is disposed between the sample and the optical gate, and the second polarizer is disposed between the optical gate and the optical analysis apparatus. The spectral signal passes through the first polarizer, the optical gate, and the second polarizer to be transmitted and received to the optical analysis apparatus when the optical gate is activated and turned on in a predetermined time period.

Abstract translation: 提供了一种包括激光装置，光分路装置，光栅，第一偏振器，第二偏振器和光学分析装置的光谱检测装置。 光分束装置将从激光装置提供的激光束分成第一和第二光束，并将第二光束传输到样品以产生光谱信号。 由第一光束激活的光栅设置在光学分析装置和样品之间。 第一偏振器设置在样品和光学栅极之间，第二偏振器设置在光学门和光学分析装置之间。 当光栅被激活并在预定时间段内接通时，光谱信号通过第一偏振器，光栅和第二偏振器，以被发射和接收到光学分析装置。

公开(公告)号：US08338858B1

公开(公告)日：2012-12-25

申请号：US13310652

申请日：2011-12-02

Applicant: Glenn Bastiaans ,  Steve Hankins ,  Jerald Alan Cole

Inventor： Glenn Bastiaans ,  Steve Hankins ,  Jerald Alan Cole

IPC: H01L31/107

CPC classification number: G01N21/7703 ,  G01J1/46 ,  G01J3/027 ,  G01J3/2889 ,  G01J3/44 ,  G01J3/4412 ,  G01J2003/1213 ,  G01N21/65 ,  G01N2021/1793

Abstract: A time correlated single photon counting system having a programmable delay generator triggered by a laser fire event detector. The system may be used for chemical agent detection based on Rayleigh scattering using optical time domain reflectometry techniques. The system may also be used for Raman detection using frequency to time transformations.

Abstract translation: 具有由激光火灾事件检测器触发的可编程延迟发生器的时间相关单光子计数系统。 该系统可用于使用光时域反射测量技术的基于瑞利散射的化学试剂检测。 该系统也可以用于使用频率到时间变换的拉曼检测。

公开(公告)号：US08305575B1

公开(公告)日：2012-11-06

申请号：US12489906

申请日：2009-06-23

Applicant: Neil Goldstein ,  Jason A. Cline ,  Pajo Vujkovic-Cvijin ,  Steven M. Adler-Golden ,  Marsha J. Fox ,  Brian Gregor ,  Jamine Lee

Inventor： Neil Goldstein ,  Jason A. Cline ,  Pajo Vujkovic-Cvijin ,  Steven M. Adler-Golden ,  Marsha J. Fox ,  Brian Gregor ,  Jamine Lee

IPC: G01J3/28

CPC classification number: G01J3/2846 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/027 ,  G01J3/2889 ,  G01N21/31 ,  G01N2021/1793

Abstract: An adaptive spectral sensor, and methods of using the sensor. The sensor uses a programmable band pass transmission filter to produce both contrast signals, which discriminate specific target materials from background materials by comparing spectral signatures in hardware, and scene radiance spectra. The adaptive spectral sensor may measure one or more scene spectra and may form a spectral image. The sensor may automatically adjust to changing spectral, spatial and temporal conditions in the environment being monitored, by changing sensor resolution in those dimensions and by changing the detection band pass. The programmable band pass can be changed on-the-fly in real time to implement a variety of detection techniques in hardware or measure the spatial or spectral signatures of specific materials and scenes.

Abstract translation: 自适应光谱传感器，以及使用传感器的方法。 传感器使用可编程带通传输滤波器来产生两个对比度信号，通过比较硬件中的光谱特征和场景辐射光谱，将特定的目标材料与背景材料区分开。 自适应光谱传感器可以测量一个或多个场景光谱并且可以形成光谱图像。 传感器可以通过改变这些维度中的传感器分辨率，并通过改变检测带通，自动调整到被监测环境中不断变化的光谱，空间和时间条件。 可实时改变可编程带通，实现硬件中的各种检测技术，或测量特定材料和场景的空间或光谱特征。

公开(公告)号：US07925069B2

公开(公告)日：2011-04-12

申请号：US12569612

申请日：2009-09-29

Applicant: William E. Ortyn ,  David A. Basiji ,  Philip Morrissey ,  Thaddeus George ,  Brian Hall ,  Cathleen Zimmerman ,  David Perry

Inventor： William E. Ortyn ,  David A. Basiji ,  Philip Morrissey ,  Thaddeus George ,  Brian Hall ,  Cathleen Zimmerman ,  David Perry

IPC: G06K9/00

CPC classification number: G01N33/505 ,  G01J3/2889 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/5005 ,  G01N2015/1006 ,  G01N2015/1497 ,  G01N2021/6421 ,  G01N2021/6441 ,  G06K9/00127

Abstract: Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.

Abstract translation: 同时收集细胞群的多峰/多光谱图像。 使用在图像中可识别的光度和/或形态测量特征将细胞群分离成多个亚群。 当细胞群体包括患病细胞和健康细胞时，图像可以分成健康亚群和患病亚群。 在细胞群不包括患病细胞的情况下，不具有疾病状况的患者中不同细胞类型的一种或多种比例可以与具有疾病状况的患者中的相应比例进行比较，从而能够检测疾病状况。 例如，血细胞可以基于它们的图像分离成不同类型，并且可以容易地检测淋巴细胞数量的增加，这是与慢性淋巴细胞性白血病相关的现象。

公开(公告)号：US07826061B2

公开(公告)日：2010-11-02

申请号：US12544313

申请日：2009-08-20

Applicant: Scott T. Sanders

Inventor： Scott T. Sanders

IPC: G01B9/02

CPC classification number: G01J5/0014 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/0291 ,  G01J3/10 ,  G01J3/2889 ,  G01J3/42 ,  G01J3/4338 ,  G01J3/4531 ,  G01J5/0018 ,  G01J5/025 ,  G01J5/08 ,  G01J5/0821 ,  G01J5/601

Abstract: A high-speed absorption spectrographic system employs a slit-less spectroscope to obtain high-resolution, high-speed spectrographic data of combustion gases in an internal combustion engine allowing precise measurement of gas parameters including temperature and species concentration.

Abstract translation: 高速吸收光谱系统采用无缝隙光谱仪，获得内燃机燃烧气体的高分辨率高速光谱数据，可精确测量包括温度和物质浓度在内的气体参数。