公开(公告)号：US5968792A

公开(公告)日：1999-10-19

申请号：US769966

申请日：1996-12-19

Applicant: Jon Douglas Wenzel ,  Kevin W. Anderson

Inventor： Jon Douglas Wenzel ,  Kevin W. Anderson

IPC: C12P7/64 ,  C12P7/62

CPC classification number: C12P7/6418

Abstract: A process for pressure splitting glycerides into fatty acids and glycerols involving the steps of: (a) providing a glyceride feedstock; (b) providing a hydrolytic lipase enzyme; (c) providing an alkaline earth metal selected from the group consisting of calcium, magnesium, and mixtures thereof; (d) mixing components (a)-(c) in the presence of water and with agitation, at a temperature ranging from about 50 to about 60.degree. C. to form a partially hydrolyzed glyceride feedstock; (e) introducing the partially hydrolyzed glyceride feedstock into a pressure splitter; and (f) splitting the partially hydrolyzed glyceride feedstock in the pressure splitter into carboxylic acids and glycerols.

Abstract translation: 将甘油酯压力分解成脂肪酸和甘油的方法，包括以下步骤：（a）提供甘油酯原料; （b）提供水解脂肪酶; （c）提供选自钙，镁及其混合物的碱土金属; （d）在水和搅拌下，在约50至约60℃的温度范围内混合组分（a） - （c），以形成部分水解的甘油酯原料; （e）将部分水解的甘油酯原料引入分压器; 和（f）将分压器中的部分水解的甘油酯原料分解成羧酸和甘油。

公开(公告)号：US5942659A

公开(公告)日：1999-08-24

申请号：US776210

申请日：1997-01-24

Applicant: Gilbert Alibert ,  Zephirin Mouloungui ,  Alain Boudet

Inventor： Gilbert Alibert ,  Zephirin Mouloungui ,  Alain Boudet

IPC: C12N9/20 ,  C12N15/55 ,  C12N15/82 ,  C12P7/64 ,  C12N5/14 ,  A01H5/00 ,  C12N15/52

CPC classification number: C12P7/6418 ,  C12N15/8242 ,  C12N15/8247 ,  C12N9/20 ,  C12P7/6436 ,  C12P7/6445 ,  C12P7/649 ,  Y02E50/13

Abstract: A process for producing fatty acids or fatty acid derivatives from oleaginous plants. This process is characterized in that transgenic oleaginous plants are produced having on the one hand at least one gene coding for a lipase enzyme, the so-called lipase gene, and on the other hand, associated with this lipase gene, a promoter permitting an expression of the gene either in compartments different from the lipid accumulation compartments, or by exogenous induction. The seeds or fruits containing the plant lipids are collected, the seeds or fruits are crushed, if necessary after induction treatment, so as to bring the lipids and lipase into contact, the whole mixture is incubated in order to effect an enzymatic hydrolysis of the lipids, and the fatty acids or derivatives thereof are extracted.

Abstract translation: PCT No.PCT / FR95 / 00957 Sec。 371日期1997年1月24日 102（e）1997年1月24日PCT PCT 1995年7月18日PCT公布。 出版物WO96 / 0351100。 日期1996年2月8日一种从含油植物生产脂肪酸或脂肪酸衍生物的方法。 该方法的特征在于，生产转基因油质植物，其一方面具有至少一种编码脂肪酶的基因，所谓的脂肪酶基因，另一方面与该脂肪酶基因相关，启动子允许表达 的基因在不同于脂肪蓄积室的隔室中，或通过外源诱导。 收集含有植物脂质的种子或果实，如果需要，诱导处理后种子或果实被粉碎，以使脂质和脂肪酶接触，将整个混合物温育以实现脂质的酶水解 ，并提取脂肪酸或其衍生物。

公开(公告)号：US5726048A

公开(公告)日：1998-03-10

申请号：US513193

申请日：1995-08-09

Applicant: Dean A. Oester ,  Allen L. Hall ,  Stephen J. Vesper

Inventor： Dean A. Oester ,  Allen L. Hall ,  Stephen J. Vesper

IPC: C11C1/04 ,  C11C3/00 ,  C11C3/02 ,  C12N1/14 ,  C12N9/20 ,  C12P7/64 ,  C12R1/645 ,  C12S3/18

CPC classification number: C12P7/6418 ,  C11C1/045 ,  C11C3/003 ,  C11C3/02 ,  C12N9/20 ,  C12P7/6427 ,  C12P7/6445 ,  C12R1/645

Abstract: A mutant fungal strain, namely G. candidum NRRL Y-552, mutant known as Henkel 9-2-3-9 produces a lipase called "UNLipase" providing a selectivity of 25:1 for oleic acid over palmitic acid by the assay procedure employed. UNLipase has a temperature range of operation of between 0 and 40 degrees Celsius (and no activity over 55 degrees Celsius). The optimum pH ranges are between 7.5 and 8.5. Magnesium cations increase activity, whereas calcium cations are inhibitory. The molecular weight of the protein appears to be 65 kDa by size exclusion chromatography. UNLipase shows a high degree of selectivity for hydrolysis, esterification and transesterification.

Abstract translation: 突变体真菌菌株，即G. candidum NRRL Y-552，称为Henkel 9-2-3-9的突变体产生称为“UNLipase”的脂肪酶，通过采用的测定程序提供油酸超过棕榈酸的25:1的选择性 。 UNLipase的操作温度范围在0到40摄氏度之间（没有活动超过55摄氏度）。 最佳pH范围为7.5至8.5。 镁阳离子增加活性，而钙阳离子是抑制性的。 蛋白质的分子量通过尺寸排阻层析显示为65kDa。 UNLipase显示出高度的水解，酯化和酯交换的选择性。

公开(公告)号：US5591615A

公开(公告)日：1997-01-07

申请号：US458317

申请日：1995-06-02

Applicant: Dean A. Oester ,  Allen L. Hall ,  Stephen J. Vesper

Inventor： Dean A. Oester ,  Allen L. Hall ,  Stephen J. Vesper

IPC: C11C1/04 ,  C11C3/00 ,  C11C3/02 ,  C12N1/14 ,  C12N9/20 ,  C12P7/64 ,  C12R1/645 ,  C12S3/18 ,  C12N15/01

CPC classification number: C12P7/6418 ,  C11C1/045 ,  C11C3/003 ,  C11C3/02 ,  C12N9/20 ,  C12P7/6427 ,  C12P7/6445 ,  C12R1/645

Abstract: A mutant fungal strain, namely G. candidum NRRL Y-552, mutant known as Henkel 9-2-3-9 produces a lipase called "UNLipase" providing a selectivity of 25:1 for oleic acid over palmitic acid by the assay procedure employed. UNLipase has a temperature range of operation of between 0 and 40 degrees Celsius (and no activity over 55 degrees Celsius). The optimum pH ranges are between 7.5 and 8.5. Magnesium cations increase activity, whereas calcium cations are inhibitory. The molecular weight of the protein appears to be 65 kDa by size exclusion chromatography. UNLipase shows a high degree of selectivity for hydrolysis, esterification and transesterification.

Abstract translation: 突变体真菌菌株，即G. candidum NRRL Y-552，称为Henkel 9-2-3-9的突变体产生称为“UNLipase”的脂肪酶，通过采用的测定程序提供油酸超过棕榈酸的25:1的选择性 。 UNLipase的操作温度范围在0到40摄氏度之间（没有活动超过55摄氏度）。 最佳pH范围为7.5至8.5。 镁阳离子增加活性，而钙阳离子是抑制性的。 蛋白质的分子量通过尺寸排阻层析显示为65kDa。 UNLipase显示出高度的水解，酯化和酯交换的选择性。

公开(公告)号：US4791059A

公开(公告)日：1988-12-13

申请号：US844389

申请日：1986-03-17

Applicant: Masahiro Nakao ,  Sumio Asami ,  Takaharu Tanaka ,  Kyoichi Ogura ,  Teruo Amachi ,  Hajime Yoshizumi ,  Hiroshi Ishigooka

Inventor： Masahiro Nakao ,  Sumio Asami ,  Takaharu Tanaka ,  Kyoichi Ogura ,  Teruo Amachi ,  Hajime Yoshizumi ,  Hiroshi Ishigooka

IPC: C12N9/20 ,  C12P7/64 ,  C12R1/44

CPC classification number: C12P7/6418 ,  C12N9/20 ,  Y10S435/882

Abstract: This invention relates to a process for preparation of a novel lipase. The process is characterized by culturing a microorganism belonging to Staphylococcus capitis and capable of producing lipase in a culture medium to accumulate lipase in the cultured broth and recovering the lipase from the broth. Since the pH of this lipase is in a slightly alkali region and the lipase substantially completely converts glyceride to fatty acids and glycerin, it is useful for the economic preparation of fatty acids by the hydrolysis of fats or oils.

Abstract translation: PCT No.PCT / JP85 / 00409 Sec。 371日期：1986年3月17日 102（e）1986年3月17日PCT申请日1985年7月19日PCT公布。 第WO86 / 00925号公报 日本1986年2月13日。本发明涉及新型脂肪酶的制备方法。 该方法的特征在于培养属于葡萄球菌属的炎症微生物，并且能够在培养基中产生脂肪酶以在培养的肉汤中积聚脂肪酶并从肉汤中回收脂肪酶。 由于该脂肪酶的pH处于微碱性区域，脂肪酶基本上完全将甘油酯转化为脂肪酸和甘油，因此通过脂肪或油的水解来经济地制备脂肪酸是有用的。

公开(公告)号：US20180094251A1

公开(公告)日：2018-04-05

申请号：US15836874

申请日：2017-12-10

Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

Inventor： Basil J. Nikolau ,  Marna Yandeau-Nelson ,  Fuyuan Jing

IPC: C12N9/16 ,  C12P7/64

CPC classification number: C12N9/16 ,  C07K2319/00 ,  C12P7/6409 ,  C12P7/6418 ,  C12Y301/02014 ,  Y02P20/52

Abstract: A method of increasing production of fatty acids comprising introducing into a host and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid molecule comprising a nucleotide sequence encoding a mutant acyl-ACP TE or a chimeric Cuphea viscosissima acyl-ACP TE; a host comprising the nucleic acid molecule; a mutant acyl-ACP TE or chimeric Cuphea viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE for at least one of its substrates comprising introducing into the plant acyl-ACP TE a substrate specificity-altering mutation; and a method of altering the level of activity of a plant acyl-ACP TE.

公开(公告)号：US09593351B2

公开(公告)日：2017-03-14

申请号：US14626505

申请日：2015-02-19

Applicant: TerraVia Holdings, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  Karen Espina ,  George Rudenko ,  Penelope Chua

IPC: C12N15/62 ,  C12P7/64 ,  C10L1/02 ,  C12N9/00 ,  C12N9/16 ,  C12P7/20 ,  C12N15/79 ,  C07C1/207 ,  C12N9/24

CPC classification number: C12P7/6427 ,  C07C1/2078 ,  C07K2319/01 ,  C10L1/02 ,  C12N9/00 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/79 ,  C12P7/20 ,  C12P7/64 ,  C12P7/6409 ,  C12P7/6418 ,  C12P7/6463 ,  C12P7/649 ,  C12Y301/02014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52 ,  Y02P60/877 ,  Y02T50/678

Abstract: Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.

Abstract translation: 本文公开了通过制备新型甘油三酯油然后化学改性油来制造可再生化学品的方法。 公开了酯交换，氢化，加氢裂化，脱氧，异构化，酯交换，羟基化，水解和皂化等方法。 还公开了含有C8，C10，C12或C14脂肪酸链长度的新型油，并且可用作本发明方法中的原料。

公开(公告)号：US20170058235A1

公开(公告)日：2017-03-02

申请号：US15308495

申请日：2015-05-15

Applicant: Novozymes A/S

Inventor： Marianne Linde Damstrup ,  Jesper Brask ,  Hanna Maria Lilbaek

IPC: C11B3/00 ,  C12N9/88 ,  C12N9/16

CPC classification number: C11B3/003 ,  A23K20/189 ,  C12N9/16 ,  C12N9/88 ,  C12P7/6418 ,  C12P7/649 ,  C12Y301/04003 ,  C12Y301/04011 ,  C12Y406/01013 ,  Y02E50/13

Abstract: The present invention relates to compositions comprising polypeptides having phospholipase C activity and to the use of such compositions for hydrolysing a phospholipid or lysophospholipid, The invention also relates to processes for hydrolysing a phospholipid or lysophospholipid, comprising contacting said phospholipid or lysophospholipid with a composition or a pluralities of polypeptides having Phospholipase C activity according to the invention.

Abstract translation: 本发明涉及包含具有磷脂酶C活性的多肽的组合物以及这种用于水解磷脂或溶血磷脂的组合物的用途。本发明还涉及水解磷脂或溶血磷脂的方法，包括使所述磷脂或溶血磷脂与组合物或 多种具有根据本发明的磷脂酶C活性的多肽。

公开(公告)号：US20160060609A1

公开(公告)日：2016-03-03

申请号：US14877335

申请日：2015-10-07

Applicant: DSM IP ASSETS B.V.

Inventor： Uwe T. BORNSCHEUER ,  David WEINER ,  Tim HITCHMAN ,  Jonathan LYON ,  Sirirung WONGSAKUL

IPC: C12N9/20 ,  A61K38/46 ,  A23L1/03 ,  D06M16/00 ,  A23K1/165 ,  C12P7/64 ,  C11D3/386

CPC classification number: C12N9/20 ,  A23C9/20 ,  A23C19/05 ,  A23C19/063 ,  A23K10/14 ,  A23K20/189 ,  A23L29/06 ,  A23V2002/00 ,  A61K8/0216 ,  A61K8/375 ,  A61K8/606 ,  A61K8/66 ,  A61K38/465 ,  A61K2800/86 ,  A61Q5/06 ,  A61Q11/00 ,  A61Q19/00 ,  C10M129/70 ,  C10M129/76 ,  C10M2207/281 ,  C10M2207/289 ,  C10M2207/40 ,  C11C3/08 ,  C11D3/386 ,  C11D3/38627 ,  C11D3/38636 ,  C12C1/047 ,  C12C5/004 ,  C12C11/003 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12P7/6418 ,  C12Q1/34 ,  C12Y301/01003 ,  D06M16/003 ,  D06M2200/12 ,  D06M2200/30 ,  D06M2200/50 ,  G01N33/573 ,  G01N2500/02

Abstract: The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phospholipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.

Abstract translation: 本发明提供水解酶，编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明涉及具有水解酶活性的多肽，例如酯酶，酰基转移酶，脂肪酶，磷脂酶（例如，磷脂酶A，B，C和D活性，patatin活性，脂质酰基水解酶（ LAH）活性）或蛋白酶活性，包括耐热和耐热水解酶活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 本发明的多肽和肽的水解酶活性包括酯酶活性，脂肪酶活性（脂质水解），酸解反应（用游离脂肪酸代替酯化脂肪酸），酯交换反应（甘油三酸酯之间的脂肪酸交换）， 酯合成，酯交换反应，磷脂酶活性和蛋白酶活性（肽键的水解）。 本发明的多肽可用于各种药物，农业和工业环境中，包括制造化妆品和营养品。 另一方面，本发明的多肽用于合成对映体纯的手性产物。

公开(公告)号：US09206448B2

公开(公告)日：2015-12-08

申请号：US13162828

申请日：2011-06-17

Applicant: Douglas Robert Anton ,  Jelena Cirakovic ,  Bruce A. Diner ,  Michael Charles Grady ,  Francis J. Woerner

Inventor： Douglas Robert Anton ,  Jelena Cirakovic ,  Bruce A. Diner ,  Michael Charles Grady ,  Francis J. Woerner

IPC: C12P1/00 ,  C12N9/00 ,  C12N15/00 ,  C12N1/14 ,  C07H21/04 ,  C12P7/16 ,  B01D3/00 ,  B01D21/26 ,  C07C29/86 ,  C11B1/02 ,  C11B13/00 ,  C12M1/00 ,  C12P7/64 ,  C12P7/18

CPC classification number: C12P7/16 ,  A23K10/38 ,  B01D3/002 ,  B01D11/0492 ,  B01D21/26 ,  B01D21/262 ,  C07C29/86 ,  C11B1/025 ,  C11B13/00 ,  C12F3/10 ,  C12M1/00 ,  C12M21/12 ,  C12M45/04 ,  C12M45/09 ,  C12N1/14 ,  C12N15/00 ,  C12P7/18 ,  C12P7/6418 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02W30/74

Abstract: In an alcohol fermentation process, oil derived from biomass is chemically converted into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be chemically converted into a reaction product, such as fatty acids, fatty alcohols, fatty amides, fatty acid methyl esters, fatty acid glycol esters, and hydroxylated triglycerides, and mixtures thereof, which forms a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be chemically converting into the fermentation product extractant. The oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be chemically converted to a fermentation product extractant, which can then contacted with a fermentation product comprising a product alcohol, whereby the product alcohol is separated from the fermentation product.

Abstract translation: 在酒精发酵过程中，将来自生物质的油化学转化成可从发酵液中原位除去产物醇如丁醇的萃取剂。 油中的甘油酯可以化学转化成反应产物，例如脂肪酸，脂肪醇，脂肪酰胺，脂肪酸甲酯，脂肪酸二醇酯和羟基化甘油三酸酯及其混合物，其形成发酵产物提取剂，其具有 产物醇的分配系数大于产物醇的生物质油的分配系数。 来自醇发酵方法的原料的油可以化学转化成发酵产物萃取剂。 在将原料进料到发酵容器之前，油可以与原料分离，并且将分离的油化学转化成发酵产物萃取剂，然后可将其与包含产物醇的发酵产物接触，由此产物醇 与发酵产物分离。