公开(公告)号：US10954469B2

公开(公告)日：2021-03-23

申请号：US16423748

申请日：2019-05-28

Applicant: Novozymes A/S

Inventor： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC: A61K38/44 ,  C11B3/00 ,  C12N9/16 ,  C12P7/64 ,  C12N9/20

Abstract: The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US09538771B2

公开(公告)日：2017-01-10

申请号：US15011920

申请日：2016-02-01

Applicant: Novozymes A/S

Inventor： Lan Tang ,  Ye Liu ,  Kim Borch ,  Jesper Brask

IPC: C12P7/64 ,  A23D9/04 ,  C11B3/00 ,  C12N9/16 ,  C12N9/20

CPC classification number: A23D9/04 ,  C11B3/003 ,  C12N9/16 ,  C12N9/20 ,  C12Y301/04003

Abstract: The present invention relates to isolated polypeptides having phospholipase C activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 本发明涉及具有磷脂酶C活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：US20210171860A1

公开(公告)日：2021-06-10

申请号：US17177349

申请日：2021-02-17

Applicant: Novozymes A/S

Inventor： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC: C11B3/00 ,  C12N9/16 ,  C12P7/64 ,  C12N9/20

Abstract: The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20200010778A1

公开(公告)日：2020-01-09

申请号：US16491093

申请日：2018-03-19

Applicant: Novozymes A/S

Inventor： Christian Hans Holm ,  Per Munk Nielsen ,  Fanny Longin ,  Sara Maria Landvik ,  Jesper Brask ,  Kim Borch ,  Robert Piotr Olinski ,  Allan Noergaard ,  Hanna Maria Lilbaek ,  Marianne Linde Damstrup ,  Tianqi Sun ,  Ming Li

IPC: C11B3/00 ,  C12N9/18

Abstract: Provided herein is about refining of vegetable oil. Further provided is the processes in which phospholipids present in the vegetable oil are hydrolysed and the oil is subsequently subject to chemical refining.

公开(公告)号：US20160289658A1

公开(公告)日：2016-10-06

申请号：US14777617

申请日：2014-03-21

Applicant: NOVOZYMES A/S

Inventor： Kim Borch ,  Sara Landvik ,  Marianne Linde Damstrup ,  Jesper Brask

IPC: C12N9/20 ,  C11B3/00

CPC classification number: C12N9/20 ,  C11B3/003 ,  C12N9/18 ,  C12Y301/01

Abstract: The present invention relates to isolated polypeptides having phospholipase A activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 本发明涉及具有磷脂酶A活性的分离多肽和编码该多肽的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：US11851630B2

公开(公告)日：2023-12-26

申请号：US17177349

申请日：2021-02-17

Applicant: Novozymes A/S

Inventor： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC: C12N9/16 ,  C11B3/00 ,  C12P7/6445 ,  C12N9/20

CPC classification number: C11B3/003 ,  C12N9/16 ,  C12N9/20 ,  C12P7/6445 ,  C12Y301/04003 ,  C12Y301/04011

Abstract: The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20190284503A1

公开(公告)日：2019-09-19

申请号：US16423748

申请日：2019-05-28

Applicant: Novozymes A/S

Inventor： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC: C11B3/00 ,  C12P7/64 ,  C12N9/20 ,  C12N9/16

Abstract: The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US10351795B2

公开(公告)日：2019-07-16

申请号：US15126098

申请日：2015-03-19

Applicant: Novozymes A/S

Inventor： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC: C12N9/20 ,  C11B3/00 ,  C12N9/16 ,  C12P7/64

Abstract: The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20130157325A1

公开(公告)日：2013-06-20

申请号：US13761633

申请日：2013-02-07

Applicant: NOVOZYMES A/S

Inventor： Leonardo De Maria ,  Jesper Brask ,  Michael Skjot ,  Shamkant Anant Patkar ,  Kim Borch ,  Allan Svendsen

IPC: C12N9/20 ,  C12P7/40 ,  C12P7/64 ,  C12P7/62

CPC classification number: C12N9/20 ,  C12P7/40 ,  C12P7/62 ,  C12P7/6418 ,  C12Y301/01003

Abstract: Molecular dynamics (MD) simulation on the three-dimensional structure of Candida anrtarctica lipase B revealed two hitherto unknown lids with a marked mobility, and this discovery was used to design lipolytic enzyme variants with increased lipolytic enzyme activity.

Abstract translation: 针对念珠菌脂肪酶B的三维结构的分子动力学（MD）模拟显示两个具有明显迁移率的未知盖，并且该发现用于设计具有增加的脂肪分解酶活性的脂肪分解酶变体。

公开(公告)号：US20240158718A1

公开(公告)日：2024-05-16

申请号：US18504863

申请日：2023-11-08

Applicant: Novozymes A/S

Inventor： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC: C11B3/00 ,  C12N9/16 ,  C12N9/20 ,  C12P7/6445

CPC classification number: C11B3/003 ,  C12N9/16 ,  C12N9/20 ,  C12P7/6445 ,  C12Y301/04003 ,  C12Y301/04011

Abstract: The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.