公开(公告)号：US12123040B2

公开(公告)日：2024-10-22

申请号：US17814337

申请日：2022-07-22

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein

IPC分类号： C12P19/26 ,  C07H1/00 ,  C07H1/06 ,  C12N1/20 ,  C12N15/72 ,  C12P19/00 ,  C12P19/12 ,  C12P19/14 ,  C12R1/19

CPC分类号： C12P19/26 ,  C07H1/00 ,  C07H1/06 ,  C12N1/205 ,  C12N15/72 ,  C12P19/00 ,  C12P19/12 ,  C12P19/14 ,  C12Y302/01 ,  C12Y302/01023 ,  C12N2800/101 ,  C12R2001/19

摘要： The present invention relates to the use of one or more glycosidases in the process for the production and/or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide.

公开(公告)号：US12065462B2

公开(公告)日：2024-08-20

申请号：US16626217

申请日：2018-06-27

申请人： GLYCOM A/S

发明人： Nikolay Khanzhin

IPC分类号： C07H1/06 ,  A23L33/00 ,  B01D61/02 ,  B01D61/04 ,  B01D61/14 ,  B01D61/58 ,  B01D69/02 ,  B01D69/12 ,  B01D71/56 ,  B01D71/58 ,  C07H3/06 ,  C08B37/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/18

CPC分类号： C07H1/06 ,  A23L33/40 ,  B01D61/027 ,  B01D61/04 ,  B01D61/146 ,  B01D61/58 ,  B01D69/02 ,  B01D69/12 ,  B01D71/56 ,  B01D71/58 ,  C07H3/06 ,  C08B37/0003 ,  C12P19/04 ,  C12P19/12 ,  C12P19/18 ,  A23L33/00 ,  A23V2002/00 ,  B01D61/145 ,  B01D2315/16 ,  B01D2325/20

摘要： The invention relates to a method for separating sialylated oligosaccharides, preferably sialylated human milk oligosaccharides (HMOs), from disaccharides, preferably lactose, produced by a fermentation or enzymatic process.

公开(公告)号：US12049476B2

公开(公告)日：2024-07-30

申请号：US18320823

申请日：2023-05-19

申请人： SANIFIT THERAPEUTICS, S.A.

发明人： Bernat Isern Amengual ,  Marco Antonio Moreno Santurino ,  Joan Perelló Bestard

IPC分类号： C07H11/04 ,  A61K9/08 ,  A61K45/06 ,  A61K47/02 ,  C07H1/06

CPC分类号： C07H11/04 ,  A61K9/08 ,  A61K45/06 ,  A61K47/02 ,  C07H1/06

摘要： The present invention provides processes for preparing soluble salts of inositol phosphate characterized by a low level of impurities. Processes for preparing soluble salts (e.g., alkali-metal or ammonium) of inositol phosphates (e.g., hexasodium or dodecasodium salts), and of inositol hexaphosphate in particular (e.g., Na6IP6 and Na12IP6), are described. Also provided are pharmaceutical compositions, methods of use, combination treatments, kits, and articles of manufacture comprising soluble salts of inositol phosphates prepared according to the method of the invention.

公开(公告)号：US12043642B2

公开(公告)日：2024-07-23

申请号：US17236501

申请日：2021-04-21

申请人： Hoffmann-La Roche Inc.

发明人： Joerg Lill ,  Filippo Sladojevich

IPC分类号： C07H1/06 ,  B01D69/02 ,  C07H21/00

CPC分类号： C07H1/06 ,  B01D69/02 ,  C07H21/00 ,  B01D2325/20

摘要： The invention relates to a new process for the purification of oligonucleotides which comprises the removal of the acid labile 5′hydroxy protecting group at the 5′-O-oligonucleotide terminus of the oligonucleotide by way of tangential flow filtration with an acidic buffer solution. The process requires less steps and allows a higher degree of automation.

公开(公告)号：US20240034747A1

公开(公告)日：2024-02-01

申请号：US18377942

申请日：2023-10-09

申请人： QINGDAO RESTORE BIOTECHNOLOGY CO., LTD.

发明人： Mengmeng Chen ,  Bingqiang Zhang ,  Yu Zhang ,  Yi Zhao ,  Yuan Zhang ,  Gaofeng Zhang ,  Xueqi Fu ,  Wei Zou

IPC分类号： C07H19/048 ,  C07H1/06 ,  C12P19/30

CPC分类号： C07H19/048 ,  C07H1/06 ,  C12P19/30

摘要： The present invention belongs to the technical field of NMN preparation, and particularly relates to a recrystallization method for NMN preparation, comprising the following steps: step 1. preparation of raw materials: preparing nicotinamide, nucleic acid, ribose-5-phosphate and phosphoric acid; step 2. preparation of reaction solution: preparing a suitable buffer, dissolving nicotinamide, ribose, ribose-5-phosphate in the buffer, and adjusting the concentration of the solution; step 3. activated phosphorylation reaction: adding phosphoric acid to the buffer of the dissolved nicotinamide and ribose, and stirring the mixture at appropriate temperature and pH conditions to make reactions take place in the mixture; step 4. addition of enzyme for catalysis; step 5. separation and purification: separating and purifying the reacted mixture to remove unreacted substrates and synthetase. The present invention is convenient for improving the crystal purity of NMN and improving the crystal quality, and is convenient for users to prepare NMN crystals.

公开(公告)号：US11884692B2

公开(公告)日：2024-01-30

申请号：US17811022

申请日：2022-07-06

申请人： Translate Bio, Inc.

发明人： Frank DeRosa ,  Anusha Dias ,  Michael Heartlein ,  Shrirang Karve

IPC分类号： C07H1/06 ,  C07H21/00 ,  C12N15/10 ,  C07H21/02

CPC分类号： C07H1/06 ,  C07H21/00 ,  C07H21/02 ,  C12N15/1017

摘要： The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of (a) precipitating mRNA from an impure preparation; (b) subjecting the impure preparation comprising precipitated mRNA to a purification process involving membrane filtration such that the precipitated mRNA is captured by a membrane; and (c) eluting the captured precipitated mRNA from the membrane by re-solubilizing the mRNA, thereby resulting in a purified mRNA solution. In some embodiments, a purification process involving membrane filtration suitable for the present invention is tangential flow filtration.

公开(公告)号：US20240026403A1

公开(公告)日：2024-01-25

申请号：US18344244

申请日：2023-06-29

申请人： Chr. Hansen HMO GmbH

发明人： Markus Helfrich ,  Stefan Jennewein

IPC分类号： C12P19/02 ,  A23L33/125 ,  A23L33/00 ,  A61K31/7012 ,  C12P19/26 ,  C07H1/08 ,  C07H3/08 ,  C07H1/06 ,  C07H7/027

CPC分类号： C12P19/02 ,  A23L33/125 ,  A23L33/40 ,  A61K31/7012 ,  C12P19/26 ,  C07H1/08 ,  C07H3/08 ,  C07H1/06 ,  C07H7/027

摘要： The present invention concerns an efficient way to isolate L-fucose from a fermentation broth. The L-fucose contained in the fermentation broth is produced by microbial fermentation (bacterial or yeasts). The inventive process comprises a step of removing biomass from the fermentation broth, a step of subjecting the resulting solution to at least one of a cationic ion exchanger treatment and an anionic ion exchanger treatment and a step of removing salts after the ion exchanger treatment. The process can provide L-fucose in powder form, in granulated form as well as in form of L-fucose crystals.

公开(公告)号：US11834691B2

公开(公告)日：2023-12-05

申请号：US16765407

申请日：2018-10-31

申请人： Chr. Hansen HMO GmbH

发明人： Stefan Jennewein ,  Markus Helfrich

IPC分类号： C12P19/02 ,  C07H1/06 ,  C07H7/027 ,  A23L33/125 ,  A23L33/00 ,  A61K31/7012 ,  C12P19/26 ,  C07H1/08 ,  C07H3/08

CPC分类号： C12P19/02 ,  A23L33/125 ,  A23L33/40 ,  A61K31/7012 ,  C07H1/06 ,  C07H1/08 ,  C07H3/08 ,  C07H7/027 ,  C12P19/26

摘要： The present invention describes an efficient way to isolate a sialic acid from a fermentation broth. The sialic acid contained in the fermentation broth is produced by bacterial fermentation. The inventive process comprises a step of removing biomass from the fermentation broth, a step of subjecting the resulting solution to at least one of a cationic ion exchanger treatment and an anionic ion exchanger treatment and a step of removing salts after the ion exchanger treatment. The process can provide the sialic acid in spray-dried form as well as in form of sialic acid crystals.

公开(公告)号：US11814618B2

公开(公告)日：2023-11-14

申请号：US17559456

申请日：2021-12-22

申请人： QIAGEN Sciences, LLC

发明人： Heather Callahan ,  Eddie Adams ,  Mark N. Brolaski ,  Victoria Nieciecki

IPC分类号： C12N15/10 ,  C07H21/02 ,  C07H1/06 ,  G01N30/88 ,  G01N30/00

CPC分类号： C12N15/1013 ,  C07H1/06 ,  C07H21/02 ,  G01N30/88 ,  G01N2030/009 ,  G01N2030/8831 ,  G01N2030/8836

摘要： Provided are methods for isolating biomolecules, such as nucleic acids and proteins, from a sample using a silica-containing surface and/or a high salt, low pH buffer.

公开(公告)号：US20230340004A1

公开(公告)日：2023-10-26

申请号：US17925613

申请日：2021-03-24

申请人： XINJIANG TECHNICAL INSTITUTE OF PHYSICS & CHEMISTRY, CHINESE ACADEMY OF SCIENCES

发明人： Haji Akber AISA ,  Guangying SUN ,  Guliqire ADILI ,  Munire ABUDUAINI ,  Yongxin ZHAO

IPC分类号： C07H1/06 ,  C07H13/10

CPC分类号： C07H13/10 ,  C07H1/06

摘要： A method for preparing high-purity granatin A: by means of a combination of low-temperature liquid phase chromatography and an isomerization conversion purification method, using pomegranate peel extract as a source material, granatin A of over 98% purity can be acquired, and can be used as a standard product for quality control of pomegranate peel, pomegranate flower, or other medicinal material with granatin A as the active ingredient.