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    Compound for Derivatizing Polypeptides and Method for Sequencing and Quantifying Amino Acids in Polypeptides Using the Same
    1.
    发明申请
    Compound for Derivatizing Polypeptides and Method for Sequencing and Quantifying Amino Acids in Polypeptides Using the Same 有权
    用于衍生多肽的化合物和使用其多肽测序和定量氨基酸的方法

    公开(公告)号：US20080220483A1

    公开(公告)日：2008-09-11

    申请号：US11630625

    申请日：2005-06-29

    申请人： Sang-Won Lee ,  Yong-Ho Lee

    发明人： Sang-Won Lee ,  Yong-Ho Lee

    IPC分类号： C12P13/04 ,  C07C331/16

    CPC分类号： C07C331/28 ,  C07B59/001 ,  C07B2200/05 ,  G01N33/6848 ,  G01N33/6851 ,  G01N2458/15

    摘要： The present invention relates to a compound for N-terminal substitution of polypeptides which is used in sequencing and quantifying amino acids and a method for sequencing and quantifying an amino acid sequence using the same. The method for sequencing and quantifying amino acids in accordance with the present invention leads to a relative quantitative analysis of proteins with very high reliability, and can distinctively discriminate between y-type ions and b-type ions on the MS/MS spectra, providing the means for realization of high-reliability protein identification.

    摘要翻译： 本发明涉及用于测序和定量氨基酸的多肽的N-末端取代的化合物，以及使用其进行氨基酸序列的测序和定量的方法。 根据本发明的用于测序和定量氨基酸的方法导致具有非常高可靠性的蛋白质的相对定量分析，并且可以区别地区分MS / MS光谱上的y型离子和b型离子，提供 用于实现高可靠性蛋白质鉴定的手段。

    System and Method for Expression Proteomics Based on Isotope Ratio Modification
    2.
    发明申请
    System and Method for Expression Proteomics Based on Isotope Ratio Modification 审中-公开
    基于同位素比率修饰的表达蛋白质组学系统与方法

    公开(公告)号：US20080038194A1

    公开(公告)日：2008-02-14

    申请号：US10597054

    申请日：2005-01-13

    申请人： Julian P. Whitelegge ,  David B. Agus ,  Jonathan Katz

    发明人： Julian P. Whitelegge ,  David B. Agus ,  Jonathan Katz

    IPC分类号： A61B5/055 ,  A61K51/00 ,  A61P43/00 ,  C07C331/16

    CPC分类号： G01N33/6848

    摘要： The present invention relates to modification of isotope ratios in peptides and polypeptides as an alternative to full isotopic exchange for coding in proteomics. Subtle modification of isotope ratio does not compromise protein identification experiments and can thus provide elemental composition data for accurate isotope ratio decoding. Application of subtle modification of isotope ratio proteomics (SMIRP) offers a convenient approach to in vivo isotope coding.

    摘要翻译： 本发明涉及肽和多肽中同位素比率的修饰，作为用于编码蛋白质组学的完全同位素交换的替代物。 同位素比率的微妙修改不会影响蛋白质鉴定实验，因此可以提供准确的同位素比率解码的元素组成数据。 微量修饰同位素比蛋白质组学（SMIRP）的应用为体内同位素编码提供了一种方便的方法。

    Preparation of substituted isothiocyanates
    7.
    发明授权
    Preparation of substituted isothiocyanates 失效
    取代的异硫氰酸酯的制备

    公开(公告)号：US3923852A

    公开(公告)日：1975-12-02

    申请号：US43304674

    申请日：1974-01-14

    申请人： STORY CHEM CORP

    发明人： ZEILER ANDREW G ,  BABAD HARRY

    IPC分类号： C07C331/16 ,  C07C161/04

    CPC分类号： C07C331/16

    摘要： Substituted isothiocyanates, such as alkyl isothiocyanates, e.g. ethyl isothiocyanate, are prepared by contacting a reaction admixture formed from an amine, such as a primary amine, and carbon disulfide with gaseous oxygen. The contacting of the reaction admixture with gaseous oxygen is carried out in the presence of a metal, such as manganese (Mn ), iron (Fe ), copper (Cu ), zinc (Zn ), nickel (Ni ) and cobalt (Co ), as an oxidation catalyst. The reaction admixture containing the amine and carbon disulfide might be an aqueous or non-aqueous reaction admixture. In the preparation of the non-aqueous reaction admixture the amine, together with the carbon disulfide employed in the make-up of the reaction admixture includes a nonaqueous, preferably a water-immiscible solvent, such as methylene chloride, benzene or carbon tetrachloride. An embodiment of this invention is indicated by the chemical equation:

    In the above-indicated chemical equation manganese chloride is employed as the oxidation catalyst and carbon tetrachloride as the reaction solvent or carrier.

    摘要翻译： 取代的异硫氰酸酯，例如烷基异硫氰酸酯，例如 异硫氰酸乙酯是通过将由胺（如伯胺）和二硫化碳形成的反应混合物与气态氧接触来制备的。 反应混合物与气态氧的接触在金属如锰（Mn ++），铁（Fe +++），铜（Cu ++），锌 （Zn ++），镍（Ni ++）和钴（Co ++））作为氧化催化剂。 含有胺和二硫化碳的反应混合物可以是水或非水反应混合物。 在非水反应混合物的制备中，胺与在反应混合物的组合中使用的二硫化碳一起包括非水，优选与水不混溶的溶剂，例如二氯甲烷，苯或四氯化碳 。 本发明的一个实施方案由化学方程式表示：MnCl 2 C 2 H 5 NH 2 + CS 2 O 2 C 2 H 5 NCS + S + H 2 O CCl 4在上述化学方程中，使用氯化锰作为氧化催化剂和四氯化碳作为反应溶剂或载体。

    Process of Extracting Isothiocyanates
    9.
    发明申请
    Process of Extracting Isothiocyanates 失效
    异硫氰酸酯提取工艺

    公开(公告)号：US20110245526A1

    公开(公告)日：2011-10-06

    申请号：US12750999

    申请日：2010-03-31

    申请人： Athula Ekanayake ,  Scott Alan Vandiest ,  Jeffrey John Kester ,  Paul Henry Zoutendam ,  Jairus R.D. David

    发明人： Athula Ekanayake ,  Scott Alan Vandiest ,  Jeffrey John Kester ,  Paul Henry Zoutendam ,  Jairus R.D. David

    IPC分类号： C07C331/16 ,  C07C331/28

    CPC分类号： A61K36/31 ,  C12P11/00 ,  C12P13/00 ,  C12Y302/01147

    摘要： A process for producing an essential oil. The essential oil can be white mustard essential oil. The white mustard essential oil can include a moisture sensitive isothiocyanate compound. The moisture sensitive isothiocyanate compound can be 4-HBITC. The essential oil can be produced from mustard seed, which can comprise a precursor sinalbin and myrosinase enzyme. The mustard seed can be reduced into a powder. Activation of the myrosinase enzyme by using a water solvent and a promoter to form a slurry can be performed, wherein the myrosinase enzyme catalyzes the production of an essential oil comprising an isothiocyanate from the sinalbin precursor.

    摘要翻译： 一种生产精油的方法。 精油可以是芥末精油。 白芥精油可以包括湿敏性异硫氰酸酯化合物。 水分敏感的异硫氰酸酯化合物可以是4-HBITC。 精油可以由芥子籽生产，其可以包含前体锡胺素和黑芥子酶。 芥菜籽可以还原成粉末。 可以进行使用水溶剂和促进剂来形成浆液来激活黑芥子酶，其中所述黑芥子酶催化从所述西宁酯前体产生包含异硫氰酸酯的精油。