公开(公告)号：US20040110247A1

公开(公告)日：2004-06-10

申请号：US10637958

申请日：2003-08-08

Inventor： Philippe Moreillon ,  Yok Ai Que ,  Steve Aellen

IPC: C12Q001/68 ,  C12Q001/18

CPC classification number: C12Q1/6888 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q1/689

Abstract: Overall ratios of ribosomal DNA and ribosomal RNA in microorganisms following exposure to an antimicrobial is shown to correspond to the presence and viability of the microorganism. Methods are provided to assess the presence and viability of microorganisms, by administering an antimicrobial to a population of microorganisms having a first and second marker, quantifying the first and second markers, and determining the ratio between the quantity of the first and second marker. A concordant result indicates the presence of viable microorganisms, whereas a discordant result indicates the presence of non-viable microorganisms.

Abstract translation: 暴露于抗微生物剂后微生物中核糖体DNA和核糖体RNA的总体比例显示与微生物的存在和存活力相对应。 提供了通过向具有第一和第二标记的微生物群体施用抗微生物剂，量化第一和第二标记物以及确定第一和第二标记物的量之间的比例来评估微生物的存在和存活力的方法。 一致的结果表明存在有活微生物，而不一致的结果表明存在非活的微生物。

公开(公告)号：US20040023290A1

公开(公告)日：2004-02-05

申请号：US10161279

申请日：2002-06-03

Inventor： John H. Griffin ,  J. Kevin Judice ,  Burton G. Christensen ,  Thomas E. Jenkins

IPC: C12Q001/68 ,  G01N033/53 ,  C12Q001/18 ,  C12N009/99 ,  A61K031/00

CPC classification number: C40B30/04 ,  A61K31/00 ,  A61K47/54 ,  A61K47/55 ,  A61K47/552 ,  A61K47/60 ,  C07C233/36 ,  C07C233/78 ,  C07C237/24 ,  C07C271/20 ,  C07C321/04 ,  C07C323/12 ,  C07C335/08 ,  C07C335/32 ,  C07C2603/18 ,  C07D207/333 ,  C07D211/58 ,  C07D215/56 ,  C07D233/90 ,  C07D235/30 ,  C07D401/06 ,  C07D401/12 ,  C07D401/14 ,  C07D405/04 ,  C07D413/14 ,  C07D471/04 ,  C07D471/14 ,  C07D473/00 ,  C07D473/34 ,  C07D475/04 ,  C07D475/08 ,  C07D487/04 ,  C07D487/06 ,  C07D493/04 ,  C07D493/06 ,  C07H19/20 ,  C12Q1/26 ,  C12Q1/44 ,  C12Q1/48 ,  C12Q1/533 ,  C40B40/04 ,  G01N33/573 ,  G01N33/6842 ,  G01N33/6845 ,  G01N33/92 ,  G01N2500/00 ,  G01N2500/04 ,  G01N2800/044 ,  Y02A50/401 ,  Y02A50/57 ,  Y10S530/807

Abstract: Novel multi-binding compounds are disclosed that modulate enzymatic processes. The compounds of the invention comprise from 2-10 ligands covalently connected, each of said ligands being capable of binding to an enzyme, enzyme substrate or enzyme cofactor thereby modulating the biological processes/functions thereof.

Abstract translation: 公开了调节酶过程的新型多结合化合物。 本发明的化合物包含共价连接的2-10个配体，每个所述配体能够结合酶，酶底物或酶辅因子，从而调节其生物学过程/功能。

公开(公告)号：US20040018983A1

公开(公告)日：2004-01-29

申请号：US10327239

申请日：2002-12-20

Applicant: Achillion Pharmaceuticals, Inc.

Inventor： William G. Rice ,  Douglas D. Buechter ,  Randall B. Murphy

IPC: A61K038/05 ,  A61K031/198 ,  A61K031/195 ,  C12Q001/18

CPC classification number: A61K31/166 ,  A61K31/00 ,  C12Q1/18

Abstract: Methods for elucidating an antifungal or anti-yeast compound which selectively bind to a fungal or yeast zinc finger-containing protein within a fungus are disclosed. Assays of screening for compounds that are effective for binding to a fungal or yeast zinc finger-containing protein are also provided. It is also provided a pharmaceutical composition containing an effective amount of a compound or compounds identified as effective for binding to or associating with a fungal or yeast zinc finger-containing protein using the disclosed methods. Fungal and/or yeast infections can be treated or prevented by administering to a patient in need of treatment the pharmaceutical composition containing an effective amount of an compound or compounds identified as effective for binding to or associating with a fungal or yeast zinc finger-containing protein using the disclosed methods.

Abstract translation: 公开了在真菌中选择性结合真菌或酵母含锌指蛋白的抗真菌或抗酵母化合物的方法。 还提供了筛选对结合真菌或酵母锌指蛋白有效的化合物的测定。 还提供了含有有效量的化合物或化合物的药物组合物，该化合物被鉴定为使用所公开的方法结合或与真菌或酵母含锌指蛋白结合。 真菌和/或酵母菌感染可以通过向需要治疗的患者施用含有有效量的化合物或被鉴定为有效结合或与真菌或酵母含锌指蛋白结合的化合物的化合物来治疗或预防 使用所公开的方法。

公开(公告)号：US20030235842A1

公开(公告)日：2003-12-25

申请号：US10374606

申请日：2003-02-26

Inventor： Martin K. R. Burnham ,  Leslie M. Palmer ,  Christopher M. Traini

IPC: C12Q001/68 ,  C07H021/04 ,  C12Q001/18 ,  C12N009/50 ,  C12P021/02 ,  C12N001/21 ,  C12N015/74

CPC classification number: C07K14/31 ,  Y02A90/24 ,  Y02A90/26

Abstract: The invention provides map polypeptides and polynucleotides encoding map polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing map polypeptides to screen for antibacterial compounds.

公开(公告)号：US20030228649A1

公开(公告)日：2003-12-11

申请号：US10313371

申请日：2002-12-06

Inventor： Christian Aichinger ,  Peter Schreier ,  Volkhart Li ,  Arnd Voerste ,  Karl-Heinz Kuck

IPC: A01N061/00 ,  C07H021/04 ,  C12Q001/26 ,  C12Q001/18 ,  C12N009/06 ,  C12N001/18 ,  C12P021/02 ,  C12N015/74

CPC classification number: C12N9/0006 ,  C12Q1/32 ,  G01N2500/00

Abstract: The invention relates to polypeptides from phytopathogenic fungi with the biological activity of an inosine monophosphate dehydrogenase, to nucleic acids encoding them, to the use of the polypeptides and nucleic acids for identifying modulators of the polypeptides, to methods for identifying such modulators, and to the use of these modulators as fungicides.

Abstract translation: 本发明涉及具有肌苷一磷酸脱氢酶的生物活性的植物病原真菌的多肽，编码它们的核酸，多肽和核酸用于鉴定多肽的调节剂的用途，以及用于鉴定这些调节剂的方法 使用这些调节剂作为杀真菌剂。

公开(公告)号：US20030224472A1

公开(公告)日：2003-12-04

申请号：US10437094

申请日：2003-05-13

Inventor： Ryan Heiniger ,  Amy Skalchunes ,  Huaqin Pan ,  Rex Tarpey ,  Jeffrey Shuster ,  Matthew M. Tanzer ,  Lisbeth Hamer ,  Kiichi Adachi ,  Todd M. DeZwaan ,  Sze-Chung Lo ,  Maria Victoria Montenegro-Chamorro ,  Sheryl Frank ,  Blaise Darveaux ,  Sanjoy Mahanty

IPC: C12Q001/48 ,  C12Q001/18

CPC classification number: C12Q1/48 ,  C12Q1/18

Abstract: The present inventors have discovered that Putrescine Aminopropyltransferase (SPE3) is essential for normal fungal pathogenicity. Specifically, the inhibition of Putrescine Aminopropyltransferase gene expression in fungi results in reduced pathogenicity on their host organism, producing smaller lesions that fail to spread across a leaf surface. Thus, Putrescine Aminopropyltransferase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit Putrescine Aminopropyltransferase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

Abstract translation: 本发明人发现，腐胺氨基丙基转移酶（SPE3）是正常真菌致病性所必需的。 具体来说，抑制真菌中的腐胺氨基丙基转移酶基因表达导致其宿主生物体的致病性降低，产生不能跨越叶表面扩散的较小损伤。 因此，腐胺氨基丙基转移酶可用作鉴定抗生素，优选抗真菌剂的靶标。 因此，本发明提供了鉴定抑制腐胺氨基丙基转移酶表达或活性的化合物的方法。 本发明的方法可用于鉴定抗生素，优选抗真菌剂。

公开(公告)号：US20030180831A1

公开(公告)日：2003-09-25

申请号：US10221800

申请日：2003-01-02

Inventor： Katsuhiko Machida ,  Sadayori Hoshina ,  Takashi Ushida ,  Junichiro Arai ,  Hideo Katayama ,  Chiaki Okumura ,  Yoshihisa Amano

IPC: C12Q001/18

CPC classification number: C12Q1/025 ,  C12Q1/18

Abstract: Microbe suspension is poured into each of the cells at a position which is in vicinity to the electrode for measurement of dissolved oxygen concentration, current from each electrode for measurement of dissolved oxygen concentration is measured at a second time interval for a third time period, each time is obtained based upon each of the measured currents at which the maximum current is obtained, each current value within a fourth time period which starts from the time at which the maximum current is obtained, drug sensitivity is detected based upon the variation condition of each current value during the fourth time period, rapid drug susceptibility measurement is realized as well.

Abstract translation: 将微生物悬浮液注入到用于测量溶解氧浓度的电极附近的位置处的每个细胞中，测量溶解氧浓度的每个电极的电流以第二时间间隔测量第三时间段 基于获得最大电流的每个测量电流获得时间，在从获得最大电流的时间开始的第四时间段内的每个电流值，基于每个电流的变化条件检测药物敏感性 在第四时期的当前值，也实现了快速的药物敏感性测量。

公开(公告)号：US20030138873A1

公开(公告)日：2003-07-24

申请号：US10054710

申请日：2002-01-22

Inventor： Koichi Masuda ,  Eugene J-M.A. Thonar ,  Brian Pfister ,  Michael Hejna

IPC: C12Q001/22 ,  C12Q001/18

CPC classification number: G01N33/5082

Abstract: A culture system and method for determining the effect of a test agent on the development, homeostasis or degradation of engineered cartilage tissue. The engineered cartilage tissue is obtained by isolating chondrogenic cells and culturing them to obtain chondrocytes in a cell-associated matrix. The chondrocytes and cell associated matrix are then cultured on a semipermeable membrane to provide the engineered cartilage tissue. The engineered tissue, or one of its precursors, can be contacted with the test agent to determine what effect, if any, the test agent has on engineered cartilage.

Abstract translation: 用于确定测试试剂对工程软骨组织的发育，稳态或降解的影响的培养系统和方法。 通过分离软骨形成细胞并培养它们以获得细胞相关基质中的软骨细胞来获得工程软骨组织。 然后将软骨细胞和细胞相关基质在半透膜上培养以提供工程化的软骨组织。 工程组织或其前体之一可与测试试剂接触，以确定测试试剂对工程软骨的影响（如果有的话）。

公开(公告)号：US20030121073A1

公开(公告)日：2003-06-26

申请号：US10305442

申请日：2002-11-26

Inventor： Peter Schreier ,  Ronald Ebbert ,  Edith Oehmen

IPC: A01H005/00 ,  C07H021/04 ,  C12Q001/18 ,  C12N009/10 ,  C12N001/16 ,  C12N001/18 ,  C12N015/82 ,  C12N015/74 ,  C12N005/04

CPC classification number: C12N9/1029

Abstract: The invention relates to nucleic acids which encode fungal polypeptides with the biological activity of acetoacetyl-CoA thiolase, to the polypeptides encoded by them and to their use as targets for fungicides and to their use for identifying new, fungicidally active compounds, and to methods of finding modulators of these polypeptides and, finally, to transgenic organisms containing sequences encoding fungal polypeptides with the function of an acetoacetyl-CoA thiolase.

Abstract translation: 本发明涉及编码具有乙酰乙酰辅酶A硫解酶的生物学活性的真菌多肽的核酸，由它们编码的多肽及其作为杀真菌剂靶的用途及其用于鉴定新的杀真菌活性化合物的方法 发现这些多肽的调节剂，最后是含有编码具有乙酰乙酰辅酶A硫解酶功能的真菌多肽的序列的转基因生物。

公开(公告)号：US20030100042A1

公开(公告)日：2003-05-29

申请号：US08608712

申请日：1996-02-29

Inventor： CHRISTINA KABBASH ,  HOWARD A. SHUMAN ,  SAMUEL C. SILVERSTEIN ,  PHYLLIS DELLA-LATTA

IPC: C12N009/00 ,  C12Q001/18 ,  C12Q001/02 ,  C12Q001/00 ,  C12Q001/26 ,  C07D201/00

CPC classification number: A61K31/192 ,  Y02A50/478 ,  Y02A50/481

Abstract: The present invention provides for a method for inhibiting growth of a bacterium which consists essentially of contacting the bacterium with a compound having the structure: 1 wherein each of R1, R2, R3, R4, R5 and R6 may be independently H, F, Cl, Br, I, nullOH, nullOR7, nullCN, nullCOR7, nullSR7, nullN(R7)2, nullNR7COR8, nullNO2, null(CH2)pOR7, null(CH2)pX(R7)2, null(CH2)pXR7COR8, a straight chain or branched, substituted or unsubstituted C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl, C3-C10 cycloalkyl, C3-C10 cycloalkenyl, thioalkyl, methylene thioalkyl, acyl, phenyl, substituted phenyl, or heteroaryl; wherein a linkage to the benzene ring may alternatively be nullNnull, nullSnull, nullOnull or nullCnull; wherein R7 or R8 may be independently H, F, Cl, Br, I, nullOH, nullCN, nullCOH, nullSH2, nullNH2, nullNHCOOH, null(CH2)pOH, null(CH2)pX(CH2), null(CH2)pXCOH, a straight chain or branched, substituted or unsubstituted C1-C10 alkyl, thioalkyl, methylene thioalkyl, acyl, phenyl, substituted phenyl, or heteroaryl; wherein A may be nullN2null, nullNHnull, nullCnullCnullCH2null, nullCnullCnullC2HOHnull, nullCnullCnullCH2null, nullCH2nullCH2nullOnull, nullCH2nullCH2nullCH2nullOnull, nullSnull, nullS(nullO)2null, nullCnullOnull, nullCnullOnullOnull, nullNHnullCnullOnull, nullCnullOnullNHnull; and wherein Q, p, N and X may independently be an integer from 1 to 10, or if Q is 1 A may be a (C1-C10)-alkyl chain, (C1-C10)-alkenyl chain or (C1-C10)-alkynyl chain which can optionally be interrupted 1 to 3 times by nullOnull or nullSnull or nullNnull; or a pharmaceutically acceptable salt or ester thereof, which compound is present in a concentration effective to inhibit growth of the bacterium. A may be an (C1-C10)-alkylene chain, (C1-C10)-alkyl chain, or (C1-C10)-alkynyl chain which is branched or unbranched, substituted or unsubstituted and can optionally be interrupted 1 to 3 times by nullOnull or nullSnull or nullNnull.

Abstract translation: 本发明提供了一种抑制细菌生长的方法，其基本上使细菌与具有以下结构的化合物接触：其中R 1，R 2，R 3，R 4，R 5和R 6各自独立地为H，F，Cl， Br，I，-OH，-OR7，-CN，-COR7，-SR7，-N（R7）2，-NR7COR8，-NO2， - （CH2）pOR7， - （CH2）pX（R7）2， - （ CH2）pXR7COR8，直链或支链的取代或未取代的C1-C10烷基，C2-C10烯基，C2-C10炔基，C3-C10环烷基，C3-C10环烯基，硫代烷基，亚甲基硫代烷基，酰基，苯基，取代的苯基， 或杂芳基; 其中与苯环的连接可以是-N，-S-，-O-或-C-; 其中R 7或R 8可以独立地是H，F，Cl，Br，I，-OH，-CN，-COH，-SH 2，-NH 2，-NHCOOH， - （CH 2）p OH， - （CH 2）p X（CH 2） - （CH 2）pXCOH，直链或支链的取代或未取代的C 1 -C 10烷基，硫代烷基，亚甲硫基烷基，酰基，苯基，取代的苯基或杂芳基; 其中A可以是-N 2 - ， - NH - ， - C = C = CH 2 - ， - C = C-C 2 HOH - ， - C = C-CH 2 - ， - CH 2 -CH 2 -O - ， - CH 2 -CH 2 -CH -O - ， - S - ， - S（= O）2 - ， - C = O-，