公开(公告)号：US20040266701A1

公开(公告)日：2004-12-30

申请号：US10873657

申请日：2004-06-21

申请人： The Regents of the University of California

发明人： William H. Fenical ,  Paul R. Jensen ,  Hak Cheol Kwon

IPC分类号： C07H017/08 ,  C12P019/62 ,  C12N009/10 ,  A61K031/7048

CPC分类号： C12P1/06 ,  A61K31/7048 ,  A61K45/06 ,  C07D321/00 ,  C07H17/08 ,  C12N1/20 ,  C12P17/08 ,  C12R1/01

摘要： A novel family of cyclic polyene natural products isolated from marine actinomycete strain CNQ140 is provided. This novel strain of actinomycetes was obtained from a previously unstudied population of marine actinomycetes that reside in sediments off La Jolla, Calif. Compounds derived from strain CNQ140 have been characterized as having a cyclic polyene-polyol structure; a molecular weight from about 996 to about 1010 in the core ring structure; and at least 58 carbons and at least 14 oxygens. The invention compounds have antitumor and/or anti-microbial activity.

摘要翻译： 提供了从海洋放线菌株CNQ140分离的环状多烯天然产物的新家族。 这种新的放线菌菌株是从加利福尼亚州拉霍亚的沉积物中的以前没有发现的海洋放线菌群体获得的，来自菌株CNQ140的化合物已被表征为具有环状多烯 - 多元醇结构; 核心环结构中分子量约996至约1010; 和至少58个碳和至少14个氧。 本发明化合物具有抗肿瘤和/或抗微生物活性。

公开(公告)号：US20040259203A1

公开(公告)日：2004-12-23

申请号：US10850125

申请日：2004-05-19

申请人： National Research Council of Canada

发明人： Michel Gilbert ,  Warren W. Wakarchuk

IPC分类号： C08B037/00 ,  C07H021/04 ,  C12N009/10

CPC分类号： C12N9/1241 ,  C12N9/1029 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1081 ,  C12P19/18

摘要： This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an null2,3- and an null2,8-activity. A null1,4-GalNAc transferase and a null1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.

摘要翻译： 本发明提供了原核糖基转移酶，包括具有α2,3-和α2,8-活性的双官能唾液酸转移酶。 本发明还提供β1,4-GalNAc转移酶和β1,3-半乳糖基转移酶，以及参与脂寡糖（LOS）合成的其它糖基转移酶和酶。 糖基转移酶可以从例如弯曲杆菌属物种获得，包括空肠弯曲杆菌。 在另外的实施方案中，本发明提供编码糖基转移酶的核酸，以及用于表达糖基转移酶的表达载体和宿主细胞。

公开(公告)号：US20040253628A1

公开(公告)日：2004-12-16

申请号：US10865813

申请日：2004-06-14

申请人： Degussa AG

发明人： Brigitte Bathe ,  Stephan Hans

IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12N009/10 ,  C12N015/74

CPC分类号： C12N9/12 ,  C07H21/04

摘要： Alleles of the lysC gene from corynebacteria that code for desensitized aspartokinases, and to processes for the preparation of L-lysine using bacteria containing these alleles.

摘要翻译： 来自编码脱敏天冬氨酸激酶的棒状杆菌的lysC基因的等位基因，以及使用含有这些等位基因的细菌制备L-赖氨酸的方法。

公开(公告)号：US20040247601A1

公开(公告)日：2004-12-09

申请号：US10781564

申请日：2004-02-18

发明人： James P. Quigley ,  John D. Hooper ,  Jacqueline E. Testa

IPC分类号： A61K039/395 ,  G01N033/574 ,  C12N009/10

CPC分类号： C07K16/30 ,  C07K14/4748 ,  G01N33/6893

摘要： The invention provides a protein that is a tumor marker protein. This protein can be used to prepare antibodies that bind to the tumor marker protein. These antibodies can be used to reduce, or eliminate metastasis by cancer cells that produce the tumor marker protein. In addition, the invention provides methods that can be used to diagnose cancer, and metastasis by cancer cells.

摘要翻译： 本发明提供了一种肿瘤标志物蛋白质。 该蛋白质可用于制备结合肿瘤标记蛋白的抗体。 这些抗体可用于减少或消除产生肿瘤标记蛋白的癌细胞的转移。 此外，本发明提供了可用于诊断癌症和癌细胞转移的方法。

公开(公告)号：US20040241645A1

公开(公告)日：2004-12-02

申请号：US09774954

申请日：2001-01-30

申请人： Genentech, Inc.

发明人： Yang Wang ,  Michael W. Spellman

IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12N009/10

CPC分类号： C12N9/1051

摘要： The present invention describes the identification, purification, recombinant production and characterization of novel O-fucosyltransferase enzymes.

摘要翻译： 本发明描述了新的O-岩藻糖基转移酶的鉴定，纯化，重组生产和表征。

公开(公告)号：US20040203113A1

公开(公告)日：2004-10-14

申请号：US10845412

申请日：2004-05-12

申请人： National Research Council of Canada

发明人： Michel Gilbert ,  Warren W. Wakarchuk

IPC分类号： C12P019/18 ,  C07H021/04 ,  C12N009/10 ,  C08B037/00

CPC分类号： C12N9/1241 ,  C12N9/1029 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1081 ,  C12P19/18

摘要： This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an null2,3- and an null2,8-activity. A null1,4-GalNAc transferase and a null1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.

摘要翻译： 本发明提供了原核糖基转移酶，包括具有α2,3-和α2,8-活性的双官能唾液酸转移酶。 本发明还提供β1,4-GalNAc转移酶和β1,3-半乳糖基转移酶，以及参与脂寡糖（LOS）合成的其它糖基转移酶和酶。 糖基转移酶可以从例如弯曲杆菌属物种获得，包括空肠弯曲杆菌。 在另外的实施方案中，本发明提供编码糖基转移酶的核酸，以及用于表达糖基转移酶的表达载体和宿主细胞。

公开(公告)号：US20040203112A1

公开(公告)日：2004-10-14

申请号：US10845408

申请日：2004-05-12

申请人： National Research Council of Canada

发明人： Michel Gilbert ,  Warren W. Wakarchuk

IPC分类号： C12P019/18 ,  C07H021/04 ,  C08B037/00 ,  C12N009/10

CPC分类号： C12N9/1241 ,  C12N9/1029 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1081 ,  C12P19/18

摘要： This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an null2,3- and an null2,8-activity. A null1,4-GalNAc transferase and a null1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.

摘要翻译： 本发明提供了原核糖基转移酶，包括具有α2,3-和α2,8-活性的双官能唾液酸转移酶。 本发明还提供β1,4-GalNAc转移酶和β1,3-半乳糖基转移酶，以及参与脂寡糖（LOS）合成的其它糖基转移酶和酶。 糖基转移酶可以从例如弯曲杆菌属物种获得，包括空肠弯曲杆菌。 在另外的实施方案中，本发明提供编码糖基转移酶的核酸，以及用于表达糖基转移酶的表达载体和宿主细胞。

公开(公告)号：US20040197880A1

公开(公告)日：2004-10-07

申请号：US10746796

申请日：2003-12-23

申请人： Daicel Chemical Industries, Ltd.

发明人： Takanori Nakajima ,  Hiroaki Yamamoto

IPC分类号： C12P013/22 ,  C07H021/04 ,  C12N009/10 ,  C12N001/21 ,  C12N015/74

CPC分类号： C12N9/80 ,  C12P13/227

摘要： The present invention provides mutant D-aminoacylases and use thereof. The mutant D-aminoacylases are hard to be inhibited by the substrate and, comprise the amino acid sequences of the D-aminoacylase derived from Alcaligenes denitrificans subsp. xylosoxydans MI-4 strain, wherein amino acid residues at specific sites have been modified. The mutants of the present invention have high reaction specificity as well as resistance to inhibition by the substrate. The present invention enables high-yield production of D-amino acids using higher concentrations of N-acyl-DL-amino acid as the substrate. The mutants of the present invention are useful in producing D-tryptophan in particular.

摘要翻译： 本发明提供了突变体D-氨基酰基酶及其用途。 突变体D-氨基酸酰基酶难以被底物抑制，并且包含衍生自产碱假单胞菌副产物的D-氨基酰化酶的氨基酸序列。 xylosoxydans MI-4菌株，其中特定位点处的氨基酸残基已被修饰。 本发明的突变体具有高反应特异性以及耐基质抑制性。 本发明能够使用更高浓度的N-酰基-DL-氨基酸作为底物，高产量生产D-氨基酸。 本发明的突变体尤其可用于生产D-色氨酸。

公开(公告)号：US20040194162A1

公开(公告)日：2004-09-30

申请号：US10835516

申请日：2004-04-28

发明人： Frederick M. Hahn ,  Adelheid R. Kuehnle

IPC分类号： A01H001/00 ,  C12N015/82 ,  C12N009/10 ,  C12N015/74

CPC分类号： C12P9/00 ,  C12N9/90 ,  C12N15/52 ,  C12N15/8209 ,  C12N15/8214 ,  C12N15/8243

摘要： Disclosed are the uses of specific genes of the mevalonate and isoprenoid biosynthetic pathways, and of inactive gene sites (the pseudogene) to (1) enhance biosynthesis of isopentenyl diphosphate, dimethylallyl diphosphate and isoprenoid pathway derived products in the plastids of transgenic plants and microalgae, (2) create novel antibiotic resistant transgenic plants and microalgae, and (3) create a novel selection system and/or targeting sites for mediating the insertion of genetic material into plant and microalgae plastids. The specific polynucleotides to be used, solely or in any combination thereof, are publicly available from GeneBank and contain open reading frames having sequences that upon expression will produce active proteins with the following enzyme activities: (a) acetoacetyl CoA thiolase (EC 2.3.1.9), (b) 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase (EC 4.1.3.5), (c) HMG-CoA reductase (EC 1.1.1.34), (d) mevalonate kinase (EC 2.7.1.36), (e) phosphomevalonate kinase (EC 2.7.4.2), (f) mevalonate diphosphate decarboxylase (EC 4.1.1.33), (g) isopentenyl diphosphate (IPP) isomerase (EC 5.3.3.2), and (h) phytoene synthase (EC 2.5.1.32).

摘要翻译： 公开了甲羟戊酸和类异戊二烯生物合成途径的特定基因和无活性基因位点（假基因）的用途，以（1）增强转基因植物和微藻质体中异戊烯基二磷酸，二甲基烯丙基二磷酸和异戊二烯通路衍生产物的生物合成， （2）创建新的抗生素抗性转基因植物和微藻，（3）创建一个新的选择系统和/或靶向位点，用于介导遗传物质插入植物和微藻质体。 待使用的单独或以任何组合形式存在的特异性多核苷酸可从GeneBank公开获得并含有具有序列的开放阅读框，其表达将产生具有以下酶活性的活性蛋白：（a）乙酰乙酰辅酶A硫解酶（EC 2.3.1.9 ），（b）3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）合酶（EC 4.1.3.5），（c）HMG-CoA还原酶（EC 1.1.1.34），（d）甲羟戊酸激酶 （e）磷酸戊糖酸盐激酶（EC 2.7.4.2），（f）甲羟戊酸二磷酸脱羧酶（EC 4.1.1.33），（g）异戊烯基二磷酸（IPP）异构酶（EC 5.3.3.2）和（h） 合成酶（EC 2.5.1.32）。

公开(公告)号：US20040161757A1

公开(公告)日：2004-08-19

申请号：US10450224

申请日：2004-02-24

发明人： Reiner Fischer ,  Eva-Maria Franken ,  Ralf Nauen ,  Ute Teuschel

IPC分类号： C12Q001/68 ,  C12Q001/48 ,  C07H021/04 ,  C12N009/10 ,  C12N005/06

CPC分类号： C12N9/93

摘要： The invention relates to nucleic acids which encode insect polypeptides with the biological activity of acetyl-CoA carboxylases, to the polypeptides encoded by them, and to their use for identifying novel, insecticidally active compounds. The invention furthermore relates to methods of finding modulators of these polypeptides, and to the use of these compounds as inhibitors of insect ACCase.

摘要翻译： 本发明涉及将编码具有乙酰-CoA羧化酶的生物活性的昆虫多肽与由其编码的多肽编码的核酸及其用于鉴定新的杀虫活性化合物的用途。 本发明还涉及寻找这些多肽的调节剂的方法，以及这些化合物作为昆虫ACCase的抑制剂的用途。