公开(公告)号：US20040219627A1

公开(公告)日：2004-11-04

申请号：US10821732

申请日：2004-04-08

Applicant: Sysmex Corporation.

Inventor： Yasuyuki Kawashima

IPC: C12Q001/02 ,  C12Q001/04

CPC classification number: C12Q1/14 ,  C12Q1/04 ,  C12Q1/10 ,  G01N15/1459 ,  G01N2015/1402

Abstract: Methods for measuring bacteria are described that include (a) fluorescently staining bacteria in a sample; (b) detecting size information from the bacteria in the sample, and fluorescence information expressing intensity of fluorescent light emitted by the bacteria; (c) creating a scattergram representing a distribution of the bacteria based on the size information and the fluorescence information detected; (d) analyzing the distribution of the bacteria in the scattergram; and (e) determining whether the bacteria in the sample is bacillus or coccus based on a result of the analyzing. Bacteria measuring apparatuses and storage media for storing computer-executable programs for analyzing bacteria are also described.

公开(公告)号：US20040142409A1

公开(公告)日：2004-07-22

申请号：US10690809

申请日：2003-10-21

Inventor： Michael John Allen ,  Lucien Ghislain

IPC: C12Q001/02 ,  C12M001/34

CPC classification number: G01N33/558 ,  C12Q1/02

Abstract: A Nanomotion Sensor comprises a micromechanical device for the detection and characterization of specimen motions when they interact with one or an array of cantilevered sensors set in the path of the moving specimen. In particular, the present invention provides a method for direct sensing and characterization of motion, including position, torsion, magnitude and direction of velocity, acceleration, force, torque, as well as binding, which may include hydrogen bonding, electrostatic attractions, hydrophobic effects, dipole interactions, or through other forces through the deflection of a micromechanical cantilever sensor. The present invention is particularly useful for the detection and characterization of the motion of biological cells such as bacteria and sperm, biological systems including motor proteins, cilia of the hearing organ and the lining of the airways in asthmatics, and microfabricated systems.

Abstract translation: 纳米运动传感器包括微机械装置，用于当它们与设置在移动样本的路径中的一个或一组悬臂式传感器相互作用时检测和表征样本运动。 特别地，本发明提供了一种用于直接感测和表征运动的方法，包括位置，扭转，幅度和方向的速度，加速度，力，扭矩以及结合，其可以包括氢键，静电吸引力，疏水效应 ，偶极相互作用，或通过其他力通过微机械悬臂传感器的偏转。 本发明特别可用于检测和表征生物细胞如细菌和精子，包括运动蛋白，听觉器官的纤毛和哮喘患者气道衬里以及微细加工系统的生物系统的运动。

公开(公告)号：US20030190693A1

公开(公告)日：2003-10-09

申请号：US09758036

申请日：2001-01-11

Inventor： Ekkehard Leberer ,  Thomas Leeuw ,  Allegra Ritscher

IPC: C12Q001/02 ,  C12N001/18 ,  C12N015/74

CPC classification number: C07K14/395

Abstract: The invention relates to processes for identifying inhibitors and activators of eukaryotic potassium channels, in which a mutated S. cerevisiae cell is used whose endogenous potassium channels TRK1, TRK2 and TOK1 are not expressed functionally, but which expresses heterologously a eukaryotic potassium channel to be studied. Other subject matters of the invention are mutated S. cerevisiae cells which do not express TRK1, TRK2 and TOK1, and the preparation and use of these mutated S. cerevisiae cells.

Abstract translation: 本发明涉及用于鉴定真核钾通道的抑制剂和活化剂的方法，其中使用其内源性钾通道TRK1，TRK2和TOK1在功能上不表达但是在异源表达待研究的真核钾通道的突变型酿酒酵母细胞 。 本发明的其它主题是突变的不表达TRK1，TRK2和TOK1的酿酒酵母细胞，以及这些突变的酿酒酵母细胞的制备和使用。

公开(公告)号：US20030096315A1

公开(公告)日：2003-05-22

申请号：US09848781

申请日：2001-05-03

Inventor： Mitchell C. Sanders

IPC: C12Q001/02 ,  C12M001/26

CPC classification number: C12Q1/04 ,  C12Q1/24 ,  C12Q1/37 ,  G01N2333/96486

Abstract: A device and method for detecting the presence or absence of a prokaryotic microorganism are provided, comprising the steps of identifying a protein, such as a microbial-specific protease that characterizes the presence of a specific prokaryotic microbe and thereby provides a marker for that microbe; detecting the protease that is a marker for the presence of a specific prokaryotic microbe by cleaving a substrate when the protease is present; and signaling the presence of that protease when cleavage has occurred. More specifically, the method comprises identifying at least one outer membrane protein or a secreted protein that is unique to a particular microbial pathogen such as for example Listeria monocytogenes and that is substrate specific.

Abstract translation: 提供了用于检测原核生物微生物存在或不存在的装置和方法，包括鉴定表征特定原核生物微生物存在的蛋白质，例如微生物特异性蛋白酶的步骤，从而提供该微生物的标记物; 通过在存在蛋白酶时通过切割底物来检测作为特异性原核生物微生物存在的标记物的蛋白酶; 并且当发生裂解时发信号通知该蛋白酶的存在。 更具体地，该方法包括鉴定至少一种特定微生物病原体（例如单核细胞增生利斯特氏菌）特有的外膜蛋白或分泌蛋白，并且其是底物特异性的。

公开(公告)号：US20020187520A1

公开(公告)日：2002-12-12

申请号：US09133766

申请日：1998-08-12

Inventor： BIRGIT ANNA HELM ,  ANNE WILSON ,  DENISE MOREIRA-MACHADO

IPC: G01N033/53 ,  G01N033/567 ,  G01N033/555 ,  C12N005/02 ,  C12N005/06 ,  C12N005/10 ,  C12Q001/02 ,  G01N033/566

CPC classification number: A61K31/70 ,  A61K38/04 ,  G01N33/5091 ,  G01N33/6854 ,  Y10S436/804 ,  Y10S436/811

Abstract: The invention relates to the use of a secretor variant cell-line expressing the alpha moiety of human IgE binding protein to determine the allergic status of a given individual. Moreover, the cell-line is also used to provide an assay system for determining the allergenicity of substances and for subsequently providing therapeutic compostions which render said substances ineffective. In addition, the invention also relates to the use of said cell-line to determine the IgE independent irritancy of substances and compositions effective for attenuating the effects of said substances.

Abstract translation: 本发明涉及使用表达人IgE结合蛋白的α部分的分泌型变体细胞系来确定给定个体的过敏状态。 此外，细胞系还用于提供用于确定物质的变应原性的测定系统，并且随后提供使所述物质无效的治疗组合物。 此外，本发明还涉及所述细胞系用于确定有效减弱所述物质的作用的物质和组合物的IgE独立刺激性的用途。

公开(公告)号：US20020137121A1

公开(公告)日：2002-09-26

申请号：US10079940

申请日：2002-02-19

Inventor： Boris Rubinsky ,  Yong Huang

IPC: C12Q001/02 ,  C12N013/00 ,  C12Q001/00

CPC classification number: G01N33/5067 ,  C12M41/46 ,  C12N13/00 ,  C12Q1/02 ,  C12Q1/025 ,  G01N33/48728 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/5014 ,  G01N33/502 ,  G01N33/5088 ,  G01N2510/00

Abstract: A method of determining information about cell viability and other characteristics relating to cell membrane permeability is disclosed. The method involves determining the effect of a cell on current flow and relating that effect to a known standard which standard may be a known healthy cell and thereby deducing the viability of the cell being tested. The cells being tested can be subjected to different environmental conditions such as surrounding chemicals, temperature, pH and pressure to determine the effects of such conditions on cell viability and/or cell permeability. The cell being tested can be in a cell suspension, grown on s ubstarte, in tissue in vitro or in tissue in vivo. The method provides substantially instantaneous results and need not include the use of dyes or other markers.

Abstract translation: 公开了一种确定细胞活力信息和与细胞膜通透性相关的其它特征的方法。 该方法包括确定细胞对电流的影响，并将该效应与已知标准相关联，该标准可以是已知的健康细胞，从而推断正在测试的细胞的存活力。 被测试的细胞可以经受不同的环境条件，例如周围的化学物质，温度，pH和压力，以确定这些条件对细胞活力和/或细胞通透性的影响。 被测细胞可以在体外或体内组织中的组织中在细胞悬液中生长。 该方法提供基本上瞬间的结果，并且不需要包括使用染料或其它标记物。

公开(公告)号：US20020127557A1

公开(公告)日：2002-09-12

申请号：US09803317

申请日：2001-03-09

Inventor： Ruoying Tan ,  Glenn Fu ,  Michael Rose ,  Juan Zhang

IPC: C12Q001/68 ,  G01N033/53 ,  C12Q001/02 ,  C12N015/00

CPC classification number: C12N15/1051

Abstract: The present invention provides a method in which cDNAs that encode signal sequences for secreted or membrane-associated proteins are isolated using a fusion protein that directs secretion of a molecule that provides antibiotic resistance, e.g., null-lactamase. The present method allows the isolation of signal peptide-associated proteins that may be difficult to isolate with other techniques. Moreover, the present method is amenable to throughput screening techniques and automation, and especially in validating the presence of the signal sequence via expression of the protein in both prokaryotic and eukaryotic cells. This invention provides a powerful and approach to the large scale isolation of novel secreted proteins.

Abstract translation: 本发明提供了一种方法，其中使用引导分泌提供抗生素抗性的分子（例如β-内酰胺酶）的融合蛋白分离编码分泌的或膜相关蛋白的信号序列的cDNA。 本方法允许分离与其它技术难以分离的信号肽相关蛋白。 此外，本发明的方法适用于吞吐量筛选技术和自动化，特别是通过蛋白质在原核和真核细胞中的表达来验证信号序列的存在。 本发明提供了大规模分离新型分泌蛋白质的强大和方法。

公开(公告)号：US20020115130A1

公开(公告)日：2002-08-22

申请号：US10078558

申请日：2002-02-14

Applicant: Universidad de Salamanca

Inventor： Jose Alberto Orfao De Matos Correia E Vale

IPC: C12Q001/02 ,  C12Q001/06

CPC classification number: G01N15/1012 ,  G01N2015/1406

Abstract: Procedure for controlling the enumeration of the absolute count of cells (or other particles) present in a sample. The procedure consists of the following: to prepare, in known quantities and proportions, a mixture or stock solution of two or more populations of reference particles of differing characteristics; to add a known quantity of this mixture of reference particles to a known volume of the sample which contains the cells (or other particles) to be counted; to measure, in a flow cytometer, the sample containing both the cells (or other particles), the events of which are to be counted, and the mixture of different populations of reference particles; to calculate the absolute number of cells (or other particles) present in the sample to be counted and; to check that the proportion between the different reference particles present in the sample measured concurs with the proportion that exists between them in the initial mixture or stock solution which contained the different populations of reference particles prior to adding it to the sample.

Abstract translation: 用于控制样品中存在的细胞（或其他颗粒）的绝对计数的计数的程序。 该方法包括以下步骤：以已知的量和比例制备具有不同特征的两个或更多个参比颗粒群的混合物或储备溶液; 将已知量的参考颗粒混合物添加到包含要计数的细胞（或其他颗粒）的样品的已知体积中; 在流式细胞仪中测量含有细胞（或其他颗粒），其事件将被计数的样品和不同参考颗粒群体的混合物; 计算待计数样品中存在的细胞（或其他颗粒）的绝对数; 以检查测量样品中存在的不同参考颗粒之间的比例与添加到样品中之前含有参考颗粒群的初始混合物或储备溶液中之间存在的比例是否一致。

公开(公告)号：US20020072052A1

公开(公告)日：2002-06-13

申请号：US09961452

申请日：2001-09-21

Inventor： Bonnie L. Bassler ,  Michael G. Surette

IPC: G01N033/554 ,  C12Q001/02 ,  C12Q001/00 ,  G01N033/569

CPC classification number: C12Q1/18 ,  A61K31/00 ,  A61K38/00 ,  C07K14/195 ,  C12Q1/68 ,  Y02A50/401 ,  Y02A50/47 ,  Y02A50/471 ,  Y02A50/57 ,  Y10S435/909 ,  Y10S530/806 ,  Y10S530/808

Abstract: The production of a purified extracellular bacterial signal called autoinducer-2 is regulated by changes in environmental conditions associated with a shift from a free-living existence to a colonizing or pathogenic existence in a host organism. Autoinducer-2 stimulates LuxQ luminescence genes, and is believed also to stimulate a variety of pathogenesis related genes in the bacterial species that produce it. A new class of bacterial genes is involved in the biosynthesis of autoinducer-2.

Abstract translation: 称为自体诱导剂-2的纯化的细胞外细菌信号的产生由与宿主生物体中的自由存活存在转移到定植或致病性存在相关的环境条件的变化来调节。 自动诱导剂-2刺激LuxQ发光基因，并且还被认为刺激产生它的细菌种类中的各种发病相关基因。 一类新的细菌基因参与了自动诱导剂-2的生物合成。

公开(公告)号：US20020068312A1

公开(公告)日：2002-06-06

申请号：US10004591

申请日：2001-12-04

Applicant: CASTELLINI S.p.A.

Inventor： Franco Castellini

IPC: C12Q001/02

CPC classification number: G01N21/534 ,  G01N17/008 ,  G01N27/06

Abstract: An apparatus for detecting biofilm in the water conduits of dental units, especially biofilm adhering to the inside surfaces of the conduits, can be used on a water line equipped with a plurality of conduits for supplying fluids to handpieces and fluid consuming units that use fluid from a main supply or accessory fluids from corresponding independent lines. A portion of one of the conduits is equipped with means for detecting the presence of the biofilm on the surfaces of the portion itself. The invention also relates to a method for detecting the biofilm. The method comprises the steps of contacting the biofilm attached to surfaces with a reagent substance or fluid; altering the biofilm by the reagent substance or fluid; and detecting the alteration that has taken place in the biofilm using the detecting means. nullFIG. 2null

Abstract translation: 用于检测牙科单元的水管道中的生物膜的装置，特别是粘附到导管内表面的生物膜的装置可用于配备有用于向手持件供应流体的多个导管的水管线和使用流体的流体消耗单元 来自相应独立线路的主要供应源或辅助流体。 一个管道的一部分装备有用于检测部分本身表面上的生物膜的存在的装置。 本发明还涉及一种用于检测生物膜的方法。 该方法包括使附着在表面上的生物膜与试剂物质或液体接触的步骤; 用试剂物质或流体改变生物膜; 并使用检测装置检测在生物膜中发生的变化。 [图。 2]