公开(公告)号：US20040224397A1

公开(公告)日：2004-11-11

申请号：US10858096

申请日：2004-06-01

Inventor： Christopher T. Culbertson ,  Stephen C. Jacobson ,  Maxine A. McClain ,  J. Michael Ramsey

IPC: C12N013/00

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12N1/066 ,  C12N13/00 ,  G01N27/44743

Abstract: Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.

Abstract translation: 公开了微流体系统和方法，其适于运输和裂解测试样品的细胞组分用于分析。 公开的使用电场破裂细胞膜的微流体系统和方法引起非常快速的裂解，从而最小化持续的细胞活性并导致细胞过程分析的更高精度。

公开(公告)号：US20040197883A1

公开(公告)日：2004-10-07

申请号：US10751586

申请日：2004-01-05

Applicant: MAXCYTE, Inc.

Inventor： Sergey M. Dzekunov ,  Linhong Li ,  Vininder Singh ,  Linda Liu ,  John W. Holaday ,  Hyung J. Lee

IPC: C12N013/00 ,  C12M001/42

CPC classification number: A61N1/0412 ,  A61K9/5068 ,  A61K48/0091 ,  A61N1/327 ,  C12M35/02 ,  C12N15/87 ,  C12N2740/10051 ,  C12N2840/203

Abstract: The present invention relates to methods and apparatus for the encapsulation of biologically-active substances in various cell populations. More particularly, the present invention relates to a method and apparatus for the encapsulation of biologically-active substances in various cell populations in blood by electroporation to achieve therapeutically desirable changes in the physical characteristics of the various cell populations in blood.

Abstract translation: 本发明涉及将生物活性物质包封在各种细胞群中的方法和装置。 更具体地，本发明涉及通过电穿孔将生物活性物质包封在血液中的各种细胞群体中以实现血液中各种细胞群体的物理特性的理想上的改变的方法和装置。

公开(公告)号：US20040029240A1

公开(公告)日：2004-02-12

申请号：US10428612

申请日：2003-05-02

Inventor： Jesse L. Acker

IPC: C12N013/00 ,  C12M001/42

CPC classification number: A61N1/327 ,  A61N1/0412 ,  A61N1/044 ,  A61N1/325 ,  C12M35/02

Abstract: A housing has a chamber containing a low speed, e.g., 6 rpm, rotating shaft of highly polished stainless steel to avoid bubbles and arcing. A fluid containing cells to be electroporated and molecules associated with the electroporation are introduced into the chamber formed by the gap between the shaft and housing in either batch or continuous flow modes. A capacitor network applies alternating positive and negative pulses of high voltage across the electrodes formed by the housing and shaft to electroporate the cells. After a few pulses, a resistive load is placed across the network to reduce the value of the pulses applied to the electrodes for a relatively longer period than the high voltage pulses to a relatively low harmless level. The relative motion of the shaft electrode and stationary housing ground electrode creates shear stress in the fluid causing the cells to change orientation to expose different cell surfaces to the voltage pulses thereby minimizing cell destruction due to excessive voltage application. The housing is cooled by thermoelectric devices to maintain the temperature of the cells at a safe level.

Abstract translation: 壳体具有包含低速，例如6rpm的高度抛光的不锈钢的旋转轴的腔室，以避免气泡和电弧。 将含有要电穿孔的细胞和与电穿孔相关联的分子的流体以间歇或连续流动模式引入由轴和壳体之间的间隙形成的室中。 电容器网络在由壳体和轴形成的电极上施加高电压的交替的正和负脉冲以对电池进行电穿孔。 在几个脉冲之后，电阻负载被放置在网络上，以将施加到电极的脉冲的值比高电压脉冲相对较长的周期减小至相对低的无害水平。 轴电极和固定壳体接地电极的相对运动在流体中产生剪切应力，导致电池改变取向以将不同的电池表面暴露于电压脉冲，从而使由于过度施加电压而引起的电池损坏最小化。 壳体由热电装置冷却以将电池的温度保持在安全水平。

公开(公告)号：US20040005694A1

公开(公告)日：2004-01-08

申请号：US10429902

申请日：2003-05-05

Inventor： Herbert Lutz

IPC: C12P001/00 ,  C12N007/02 ,  C12N013/00

CPC classification number: A61L2/08 ,  A61L2/0005 ,  A61L2/0011 ,  A61L2/10 ,  A61L2202/122 ,  C12M29/26

Abstract: Fermentation media, employed for its ability to grow recombinant mammalian cells to high densities and abet their expression of recombinant protein drug products at high titers, are rendered free of active viruses by treatment with low levels of light, levels at which the essential media properties are retained.

Abstract translation: 用于将重组哺乳动物细胞生长至高密度并以高效价使其重组蛋白质药物表达的能力的发酵培养基通过用低水平的光处理而得不到活性病毒，其中必需培养基性质为 保留。

公开(公告)号：US20030138922A1

公开(公告)日：2003-07-24

申请号：US10058480

申请日：2002-01-24

Inventor： William John Martin

IPC: C12Q001/70 ,  C12P001/00 ,  C12N013/00 ,  C12N007/00 ,  C12N007/01

CPC classification number: C12Q1/705

Abstract: A method of causing irreversible damage to stealth virus infected cells, based on exposing the infected cells to magnetic energy. The method of the present application specifically relates to the culturing of stealth viruses from an infected subject and determining the presence of magnetic and/or paramagnetic material in the stealth virus infected culture. Magnetic and/or paramagnetic material can also be found in cultures of bacteria that are infected with the patient's stealth virus. The cultured stealth virus infected cells are tested for susceptibility to cell damage caused by a magnetic field that is not significantly injurious to normal uninfected cells. The presence of magnetic and/or paramagnetic material in the stealth virus culture and the demonstration that exposure to a strong magnetic field can cause damage to stealth virus infected cells, form the basis of therapy for stealth virus infected patients and animals. The therapy comprises exposing stealth virus infected cells within a patient to a magnetic field of sufficient strength to cause damage to cultured virus infected cells. Magnetic energy therapy is especially applicable as therapy for stealth virus associated cancers in which the stealth virus isolated from the cancer patient can be shown to induce the formation of magnetic and/or paramagnetic material.

Abstract translation: 基于将受感染的细胞暴露于磁能，引起隐形病毒感染细胞的不可逆损伤的方法。 本申请的方法具体涉及从受感染的受试者中培养隐形病毒并确定隐形病毒感染培养物中磁性和/或顺磁材料的存在。 磁性和/或顺磁性材料也可以在被病毒隐形病毒感染的细菌培养物中发现。 测试培养的隐形病毒感染细胞对不受正常未感染细胞显着损伤的磁场引起的细胞损伤的易感性。 在隐形病毒培养物中存在磁性和/或顺磁材料，并且证明暴露于强磁场可能会对隐形病毒感染的细胞造成伤害，形成隐形病毒感染患者和动物的治疗基础。 该疗法包括将患者内的隐形病毒感染的细胞暴露于具有足够强度的磁场以对培养的病毒感染的细胞造成损害。 磁能治疗特别适用于隐形病毒相关癌症的治疗，其中可以显示从癌症患者分离的隐形病毒诱导形成磁性和/或顺磁性材料。

公开(公告)号：US20030064487A1

公开(公告)日：2003-04-03

申请号：US10192805

申请日：2002-07-09

Applicant: Ultra Biotech Limited

Inventor： Lingyu Zhang

IPC: C12N013/00 ,  A01N063/00 ,  A01N063/04

CPC classification number: C05F11/08 ,  C12N1/14 ,  C12N13/00

Abstract: The present invention provides biological fertilizer compositions that comprise yeast cells that have an enhanced ability to fix atmospheric nitrogen, decompose phosphorus minerals and compounds, decompose potassium minerals and compounds, decompose complex carbon compounds, over produce growth factors, and over produce ATP. The biological fertilizer composition of the invention can replace mineral fertilizers in supplying nitrogen, phosphorus, and potassium to crop plants. Methods of manufacturing the biological fertilizer compositions and methods of uses are also encompassed.

公开(公告)号：US20030049810A1

公开(公告)日：2003-03-13

申请号：US10269772

申请日：2002-10-10

Inventor： Darrell P. Chandler ,  Gerald J. Posakony ,  Leonard J. Bond ,  Cynthia J. Bruckner-Lea

IPC: C12N013/00 ,  C12M001/00

CPC classification number: C12M45/02 ,  B01J19/10 ,  B01J19/2415 ,  B01J2219/00033 ,  B01J2219/00036 ,  B01J2219/00058 ,  B01J2219/00148 ,  B01J2219/00166 ,  B01J2219/1921 ,  B01J2219/1923 ,  B01J2219/1943 ,  C12M47/06

Abstract: The present invention is an apparatus and method for ultrasonically treating a liquid to generate a product. The apparatus is capable of treating a continuously-flowing, or intermittently-flowing, liquid along a line segment coincident with the flow path of the liquid. The apparatus has one or more ultrasonic transducers positioned asymmetrically about the line segment. The ultrasonic field encompasses the line segment and the ultrasonic energy may be concentrated along the line segment. Lysing treatments have been successfully achieved with efficiencies of greater than 99% using ultrasound at MHz frequencies without erosion or heating problems and without the need for chemical or mechanical pretreatment, or contrast agents. The present invention overcomes drawbacks of current ultrasonic treatments beyond lysing and opens up new sonochemical and sonophysical processing opportunities.

Abstract translation: 本发明是用于超声处理液体以产生产品的装置和方法。 该装置能够沿着与液体的流动路径重合的线段处理连续流动或间歇流动的液体。 该装置具有一个或多个超声换能器，其围绕线段不对称地定位。 超声波场包围线段，超声能量可以沿着线段集中。 使用超声波在MHz频率下的效率大于99％，无侵蚀或加热问题，无需化学或机械预处理或造影剂，​​已成功实现了裂解处理。 本发明克服了目前超声波处理超声裂纹的缺点，开辟了新的声化学和声体处理机会。

公开(公告)号：US20030049809A1

公开(公告)日：2003-03-13

申请号：US10196020

申请日：2002-07-16

Inventor： Klaus Kaiser ,  Hans-Juergen Henzler ,  Joerg Kauling ,  Rolf Treckmann ,  Kathryn Remington ,  Cynthia J. Galloway

IPC: C12N013/00 ,  A61L002/12

CPC classification number: B01J19/123 ,  A23L3/28 ,  A61K41/0019 ,  A61L2/0005 ,  A61L2/0011 ,  A61L2/10 ,  A61M1/3681 ,  A61M1/3683 ,  A61M2205/053 ,  B01J19/2405 ,  B01J2219/00139 ,  B01J2219/00164 ,  B01J2219/00168 ,  C02F1/325 ,  C02F2201/32 ,  C02F2201/3223 ,  C02F2201/328 ,  Y10S422/905

Abstract: A method of inactivating microorganisms such as viruses within a fluid such as a biological fluid is disclosed. The method includes the steps of providing a UV reactor, which may take the form of an elongated generally annular reaction chamber surrounding at least one elongated UV lamp, moving the fluid within the reaction chamber in a primary flow directed along the length of the UV lamp, and inducing a circulating secondary flow within the fluid with the secondary flow being superimposed on the primary flow. As the fluid moves through the reaction chamber in the primary flow, it is circulated repeatedly toward and away from the UV lamp in the circulating secondary flow to provide uniform and controllable exposure of the entire volume of fluid to ultraviolet radiation. Microorganisms such as viruses are thus inactivated while desirable components in the fluid, such as proteins, are preserved without the use of a free radical scavenger.

Abstract translation: 公开了一种灭活诸如生物流体之类的流体中的微生物如病毒的方法。 该方法包括以下步骤：提供UV反应器，UV反应器可采取围绕至少一个细长UV灯的细长的大致环形的反应室的形式，使反应室内的流体以沿着UV灯长度的一次流动 并且在所述流体中引起循环次级流，其中所述次流叠加在所述主流上。 当流体在初级流中移动通过反应室时，它在循环的二次流中反复地朝向和远离UV灯循环，以提供整个体积的流体对紫外线辐射的均匀和可控的曝光。 因此，微生物如病毒灭活，而流体中的期望组分（例如蛋白质）在不使用自由基清除剂的情况下被保存。

公开(公告)号：US20020168764A1

公开(公告)日：2002-11-14

申请号：US10097183

申请日：2002-03-14

Applicant: Oncolytics Biotech, Inc.

Inventor： Matthew C. Coffey ,  Bradley G. Thompson

IPC: C12N013/00 ,  C12N007/00 ,  C12N007/01 ,  C12N007/02 ,  C12N005/00 ,  C12N005/02

CPC classification number: C12N7/00 ,  A61K35/765 ,  C12N2720/12232 ,  C12N2720/12251

Abstract: The present invention is directed to a method of extracting virus, particularly reovirus, from a culture of cells. Infectious virus can be extracted from the culture with a detergent at a convenient temperature such as 25null C. or 37null C. to produce high virus titers. Both ionic and non-ionic detergents can be used in the present invention.

Abstract translation: 本发明涉及从细胞培养物中提取病毒，特别是呼肠弧病毒的方法。 感染性病毒可以用诸如25℃或37℃的方便温度的洗涤剂从培养物中提取，以产生高病毒效价。 离子和非离子洗涤剂都可用于本发明。

公开(公告)号：US20020108869A1

公开(公告)日：2002-08-15

申请号：US09779955

申请日：2001-02-09

Inventor： Alex Savtchenko

IPC: C12N013/00

CPC classification number: G01N33/48728

Abstract: A device to facilitate electrophysiological measurements of a biological material comprises a plate having a plurality of wells that each have an end. At least some of the wells have a hole formed in the end, and the holes are configured to receive an individual cell such that a high resistance seal is formed between the cell and the end. A chamber is disposed adjacent the plate and is in fluid communication with each of the holes. A common electrode is disposed in the chamber, and a plurality of well electrodes are configured to be positioned within the wells. In this way, a voltage gradient may be created across cell membranes of cells that are positioned within the holes so that electrophysiological measurements of the cells may be taken.

Abstract translation: 促进生物材料的电生理测量的装置包括具有多个孔的板，每个孔各有一个端部。 孔中的至少一些具有形成在该端部的孔，并且孔构造成接收单个电池，使得在电池和端部之间形成高电阻密封。 腔室邻近板设置并与每个孔流体连通。 公共电极设置在室中，并且多个阱电极被配置为位于孔内。 以这种方式，可以在位于孔内的细胞的细胞膜上产生电压梯度，从而可以进行细胞的电生理测量。