公开(公告)号：US20040152183A1

公开(公告)日：2004-08-05

申请号：US10470604

申请日：2004-03-02

Inventor： Catherine E. O'Riordan ,  Amy L. Helgerson ,  Alan E. Smith

IPC: C12N007/02

CPC classification number: C12N7/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10351 ,  C12N2750/14143 ,  C12N2750/14151 ,  Y10S435/803

Abstract: The present invention relates to the purification of large scale quantities of active (infectious) adenovirus and AAV, especially for use in therapeutic applications. In particular, the invention provides improved methods for contacting such viruses with suitable chromatographic materials in a fashion such that any damage to the virus, particularly to surface components thereof, resulting from contact with such chromatographic materials is minimized or eliminated. The result is the ability to rapidly and efficiently purify commercial level quantities of active (infectious) virus suitable for use in therapeutic applications, e.g. gene transfer/therapy procedures.

Abstract translation: 本发明涉及大规模量的活性（感染性）腺病毒和AAV的纯化，特别是用于治疗应用。 特别地，本发明提供了使这种病毒与合适的色谱材料接触的改进方法，使得与这种色谱材料的接触导致的对病毒，特别是其表面组分的任何损害被最小化或消除。 其结果是能够快速有效地纯化商业级数量的适用于治疗应用的活性（感染性）病毒，例如， 基因转移/治疗程序。

公开(公告)号：US20040152182A1

公开(公告)日：2004-08-05

申请号：US10770467

申请日：2004-02-04

Inventor： Bodo Plachter

IPC: C12Q001/70 ,  A61K039/00 ,  C12N007/01 ,  C07K014/00 ,  C12N015/86 ,  C12N007/02 ,  A61K039/12 ,  A61K039/25 ,  C07K001/00 ,  A61K039/245 ,  C12N007/00 ,  C07K017/00

CPC classification number: C12N15/86 ,  A61K2039/525 ,  C07K14/005 ,  C07K2319/00 ,  C12N7/00 ,  C12N2710/16122 ,  C12N2710/16143 ,  C12N2710/16162

Abstract: This invention relates to viral particles which are released by mammal cells after the infection with human cytomegalovirus (HCMV). The invention also relates to particles whose antigenicity has been optimized by changing the HCMV using genetic engineering. The invention further relates to the use of such particles as a vaccine and a method for multiplying the HCMV in mammal cells.

Abstract translation: 本发明涉及在人巨细胞病毒（HCMV）感染后由哺乳动物细胞释放的病毒颗粒。 本发明还涉及通过使用遗传工程改变HCMV来优化其抗原性的颗粒。 本发明还涉及这样的颗粒作为疫苗的使用以及在哺乳动物细胞中使HCMV倍增的方法。

公开(公告)号：US20040110266A1

公开(公告)日：2004-06-10

申请号：US10166347

申请日：2002-05-17

Inventor： John A. Chiorini ,  Nikola K. Kaludov

IPC: C12N007/02

CPC classification number: C12N7/00 ,  C12N2750/14151

Abstract: The present invention provides methods of purifying adeno-associated virus (AAV) particles. These AAV particles include AAV2, AAV4 and AAV5 particles. The present invention also provides AAV particles purified by the methods of the present invention.

Abstract translation: 本发明提供了纯化腺相关病毒（AAV）颗粒的方法。 这些AAV颗粒包括AAV2，AAV4和AAV5颗粒。 本发明还提供了通过本发明的方法纯化的AAV颗粒。

公开(公告)号：US20040005694A1

公开(公告)日：2004-01-08

申请号：US10429902

申请日：2003-05-05

Inventor： Herbert Lutz

IPC: C12P001/00 ,  C12N007/02 ,  C12N013/00

CPC classification number: A61L2/08 ,  A61L2/0005 ,  A61L2/0011 ,  A61L2/10 ,  A61L2202/122 ,  C12M29/26

Abstract: Fermentation media, employed for its ability to grow recombinant mammalian cells to high densities and abet their expression of recombinant protein drug products at high titers, are rendered free of active viruses by treatment with low levels of light, levels at which the essential media properties are retained.

Abstract translation: 用于将重组哺乳动物细胞生长至高密度并以高效价使其重组蛋白质药物表达的能力的发酵培养基通过用低水平的光处理而得不到活性病毒，其中必需培养基性质为 保留。

公开(公告)号：US20030134404A1

公开(公告)日：2003-07-17

申请号：US10304828

申请日：2002-11-26

Inventor： Michael A. Lochrie ,  Peter Colosi

IPC: C12N007/02

CPC classification number: C12N15/86 ,  C12N7/00 ,  C12N2750/14143 ,  C12N2750/14151

Abstract: Methods for removing empty capsids from stocks of AAV virions comprising mixtures of empty and packaged capsids are described. The methods entail heating and adjusting the pH value of the stock, optionally in the presence of one or more chemical destabilizing agents.

Abstract translation: 描述了从包含空衣壳和包装衣壳的混合物的AAV病毒颗粒的库存中去除空的衣壳的方法。 该方法需要加热和调节原料的pH值，任选地在一种或多种化学破坏稳定剂的存在下。

公开(公告)号：US20030104441A1

公开(公告)日：2003-06-05

申请号：US10239172

申请日：2002-09-25

Inventor： Anders Malmsten ,  Ingvar Pettersson ,  Tommy Gatu ,  Clas Kallander ,  Simon Gronowitz

IPC: C12Q001/70 ,  C12Q001/68 ,  C12N007/02

CPC classification number: C07K14/005 ,  C12N7/00 ,  C12N2740/15022 ,  C12N2740/15051 ,  C12N2740/16222 ,  C12Q1/48 ,  G01N2333/16 ,  G01N2333/9125

Abstract: A method of concentrating and recovering an enzyme activity from enveloped viruses present in a biological sample, is described. The method comprises contacting the biological sample in a first buffer solution with a virus-binding matrix, such as an anion exchanger matrix, to attach virus particles present in the sample to the matrix, washing the matrix carrying the virus particles with a second buffer solution to remove components interfering with viral enzyme activity, lysing the immobilized virus particles in a third buffer solution and recovering the concentrated viral enzyme activity from the third buffer solution. Additionally, a commercial package containing written and/or data carrier instructions for performing laboratory steps for concentration and recovery of an enzyme activity from enveloped viruses present in a biological sample and at least one component necessary for the assay, is disclosed.

Abstract translation: 描述了存在于生物样品中的包膜病毒浓缩和回收酶活性的方法。 该方法包括使第一缓冲溶液中的生物样品与病毒结合基质如阴离子交换剂基质接触，将存在于样品中的病毒颗粒附着于基质中，用第二缓冲溶液洗涤携带病毒颗粒的基质 去除干扰病毒酶活性的组分，在第三缓冲溶液中裂解固定的病毒颗粒并从第三缓冲液中回收浓缩的病毒酶活性。 另外，公开了一种包含书写和/或数据载体说明书的商业包装，用于执行实验室步骤以浓缩和回收存在于生物样品中的包膜病毒的酶活性和至少一种该测定所必需的成分。

公开(公告)号：US20030099669A1

公开(公告)日：2003-05-29

申请号：US10338164

申请日：2003-01-06

Inventor： Kye-Hyung Paik

IPC: A61K039/12 ,  C12P021/02 ,  C12N005/06 ,  C12N015/86 ,  C12N005/08 ,  C12N007/02

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2730/10122 ,  C12N2730/10143 ,  C12N2770/24222 ,  C12N2770/32422 ,  C12N2799/021 ,  C12N2800/108 ,  C12P21/02 ,  Y02A50/464

Abstract: A method of producing viral antigens in vitro by infecting animal organ tissue rich in mitochondria with a virus, including human hepatitis B virus (HBV), and culturing the infected tissue in vitro is disclosed. A method of producing proteins in vitro by transfecting mitochondria-rich animal tissue with a recombinant HBV-based vector and culturing the transfected tissue in a dynamic tissue culture system is disclosed.

Abstract translation: 公开了一种通过用包含人乙型肝炎病毒（HBV）的病毒感染富含线粒体的动物器官组织并在体外培养感染组织体外生产病毒抗原的方法。 公开了一种通过用重组的HBV基载体转染富含线粒体的动物组织并在动态组织培养系统中培养转染的组织体外产生蛋白质的方法。

公开(公告)号：US20030095984A1

公开(公告)日：2003-05-22

申请号：US10213965

申请日：2002-08-07

Applicant: SMITHKLINE BEECHAM BIOLOGICALS (S.A.)

Inventor： Nigel Maurice Harford ,  Brigitte Desiree Alberte Colau ,  Jean Didelez

IPC: A61K039/295 ,  A61K039/165 ,  C12N007/00 ,  C12N007/01 ,  C12N007/02

CPC classification number: C07K14/005 ,  A61K39/0015 ,  A61K39/12 ,  A61K39/165 ,  A61K2039/5254 ,  A61K2039/70 ,  C12N7/00 ,  C12N2760/18434 ,  C12N2760/18721 ,  C12N2760/18722 ,  C12N2760/18734 ,  C12N2760/18762 ,  C12N2770/36234 ,  Y02A50/407

Abstract: A new mumps vaccine is presented, comprising a homogeneous pure isolate derived from the Jeryl-Lynn strain of mumps virus.

Abstract translation: 提出了一种新的腮腺炎疫苗，其包含源自腮腺炎病毒的Jeryl-Lynn菌株的均质纯分离物。

公开(公告)号：US20030049830A1

公开(公告)日：2003-03-13

申请号：US10257229

申请日：2002-10-10

Inventor： Karen Metcalfe

IPC: C07H021/04 ,  C12N007/01 ,  C12N007/02 ,  C12N015/09 ,  C12N015/70 ,  C12N007/00 ,  C12N007/04 ,  C12N015/00 ,  C12N015/63 ,  C12N015/74

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/245 ,  A61K2039/5254 ,  C07K14/005 ,  C12N2710/16622 ,  C12N2710/16634 ,  C12N2710/16652 ,  C12N2710/16661

Abstract: The present invention is directed to a cell line capable of supporting replication of a growth-defective Herpes Simplex Virus strain; specifically a replication-defective HSV-2 double mutant. Particularly disclosed is a cell line that expresses the ICP8 protein and the UL5 protein of Herpes Simplex Virus. This cell line is useful to propagate a replication-defective HSV-2 vaccine strain that contains mutations and/or deletions in the ICP8 and UL5 genes.

Abstract translation: 本发明涉及能够支持生长缺陷型单纯疱疹病毒株复制的细胞系; 具体是复制缺陷型HSV-2双突变体。 特别公开的是表达单克隆病毒的ICP8蛋白和UL5蛋白的细胞系。 该细胞系可用于繁殖在ICP8和UL5基因中含有突变和/或缺失的复制缺陷型HSV-2疫苗株。

公开(公告)号：US20030040100A1

公开(公告)日：2003-02-27

申请号：US09911020

申请日：2001-07-23

Applicant: GenVec, Inc.

Inventor： Douglas E. Brough ,  Imre Kovesdi

IPC: C12N007/02 ,  C12N005/02 ,  C07H021/04 ,  C12N005/00 ,  C12N007/01 ,  C12N007/00

CPC classification number: C12N15/86 ,  C12N7/00 ,  C12N2710/10321 ,  C12N2710/10343 ,  C12N2710/10352

Abstract: The invention provides a cell and a method of using the cell for the propagation of a replication-deficient adenoviral vector, wherein the cellular genome comprises a nucleic acid sequence whose expression produces a gene product that complements a replication-deficient adenoviral vector. The nucleic acid sequence is operatively linked to a chimeric expression control sequence comprising at least a functional portion of a CMV immediate early promoter/enhancer region and/or at least a functional portion of an adenoviral promoter, wherein the chimeric expression control sequence is upregulated by one or more viral proteins not produced by the nucleic acid sequence.

Abstract translation: 本发明提供了一种细胞和使用该细胞用于增殖复制缺陷型腺病毒载体的方法，其中所述细胞基因组包含其表达产生补充复制缺陷型腺病毒载体的基因产物的核酸序列。 核酸序列可操作地连接到包含CMV立即早期启动子/增强子区的至少一个功能部分和/或腺病毒启动子的至少功能部分的嵌合表达控制序列，其中嵌合表达控制序列被上调 不是由核酸序列产生的一种或多种病毒蛋白。