知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

46 条记录 (耗时 0.017 秒)

    Expression of recombinant human proteins in Tetrahymena
    1.
    发明申请
    Expression of recombinant human proteins in Tetrahymena 有权
    重组人蛋白在四膜虫中的表达

    公开(公告)号：US20030219869A1

    公开(公告)日：2003-11-27

    申请号：US10395433

    申请日：2003-03-24

    申请人： NUTRINOVA Nutrition Specialties & Food Ingredients GmbH

    发明人： Thomas Kiy ,  Matthias Rusing

    IPC分类号： C12P021/02 ,  C12N001/10 ,  C12N015/74

    CPC分类号： C07K14/765 ,  C12N15/79

    摘要： The present invention concerns a method for production of recombinant human proteins, in which Tetrahymena cells are transformed with recombinant DNA containing at least one functional gene that codes for a human protein, the recombinant Tetrahymena cells are cultured, in which the gene that codes for a human protein is expressed and the proteins are then isolated. The present invention also concerns a corresponding method, in which the gene that codes for a human protein contains a human leader sequence that leads to secretion of the expressed protein.

    摘要翻译： 本发明涉及重组人蛋白质的制备方法，其中用包含编码人蛋白质的至少一个功能基因的重组DNA转化四膜虫细胞，培养重组四膜虫细胞，其中编码 表达人蛋白质，然后分离蛋白质。 本发明还涉及一种相应的方法，其中编码人蛋白质的基因含有导致表达的蛋白质分泌的人前导序列。

    Improved Methods for producing oocysts
    3.
    发明申请
    Improved Methods for producing oocysts 有权
    改进生产卵囊的方法

    公开(公告)号：US20030143717A1

    公开(公告)日：2003-07-31

    申请号：US10232204

    申请日：2002-08-29

    发明人： James E. Hutchins ,  Julius K. Tyczkowski

    IPC分类号： C12N001/10

    CPC分类号： A61K39/012 ,  A61K2039/52 ,  C12N1/10 ,  Y10T436/25 ,  Y10T436/255 ,  Y10T436/25625

    摘要： The present invention provides improved methods and compositions for producing oocysts. The oocysts produced according to the invention find use in the manufacture of vaccines. In preferred embodiments, the present invention provides methods and compositions for the production of Eimeria oocysts. Vaccines containing Eimeria oocysts, sporocysts and/or sporozoites produced according to the present invention may be used to immunize birds against coccidiosis either in ovo or post hatch.

    摘要翻译： 本发明提供了用于生产卵囊的改进的方法和组合物。 根据本发明生产的卵囊用于制造疫苗。 在优选的实施方案中，本发明提供用于生产艾美球虫卵囊的方法和组合物。 根据本发明生产的含艾美球虫卵囊，孢子囊和/或子孢子的疫苗可以用于在卵内或后孵化中免疫鸟类针对球虫病。

    Method of rapidly generating double-stranded RNA and methods of use thereof
    5.
    发明申请
    Method of rapidly generating double-stranded RNA and methods of use thereof 审中-公开
    快速产生双链RNA的方法及其使用方法

    公开(公告)号：US20020182223A1

    公开(公告)日：2002-12-05

    申请号：US09870407

    申请日：2001-05-30

    发明人： Douglas J. LaCount ,  John E. Donelson

    IPC分类号： A61K039/002 ,  C12N015/74 ,  C12N001/10

    CPC分类号： C12N15/74 ,  A61K39/00 ,  A61K2039/515 ,  A61K2039/53

    摘要： The present invention provides eukaryotic double-stranded RNA (dsRNA) expression vectors containing two promoters directed head-to-head with a designated intervening sequence of interest that is effective in eukaryotic cells containing the dsRNA expression vector, and a vaccine using an attenuated eukaryotic pathogenic cell. The present invention also provides methods of vaccinating against colonization or infection of a eukaryotic pathogen. The present invention further provides methods of generating double-stranded RNA (dsRNA) and screening designated nucleic acids capable of inhibiting expression of an essential eukaryotic gene.

    摘要翻译： 本发明提供了含有两个启动子的真核双链RNA（dsRNA）表达载体，所述启动子以指定的感兴趣的间插序列头对头，其在含有dsRNA表达载体的真核细胞中有效，以及使用减毒的真核病原体 细胞。 本发明还提供了针对真核病原体的定植或感染疫苗接种的方法。 本发明还提供了产生双链RNA（dsRNA）并筛选能够抑制必需真核基因表达的指定核酸的方法。

    Method for enhancing levels of polyunsaturated fatty acids in thraustochytrid protists
    6.
    发明申请
    Method for enhancing levels of polyunsaturated fatty acids in thraustochytrid protists 审中-公开
    增强破裂症原体质中多不饱和脂肪酸含量的方法

    公开(公告)号：US20040203120A1

    公开(公告)日：2004-10-14

    申请号：US10393366

    申请日：2003-03-21

    申请人： Council of Scientific and Industrial Research

    发明人： Seshagiri Raghu Kumar ,  Ruchi Jain

    IPC分类号： C12P007/64 ,  C12N001/10

    CPC分类号： C12P7/6427 ,  C12P7/6472

    摘要： The present invention relates to a method of enhancing the levels of Poly-unsaturated fatty acids (PUFA) by 0.5 to 5 times in protests thraustochytrid protests by storing the cells under cold conditions, said method comprising steps of inoculating the protests into a culture medium, growing the culture for 2 to 5 days at room temperature, harvesting the cells by centrifugation to obtain biomass, storing the biomass at about 10null C. for time duration ranging between 12 to 48 hours, and obtaining increased PUFA from the stored biomass.

    摘要翻译： 本发明涉及一种通过在冷条件下储存细胞来提高抗坏血酸破坏性抗原活动中多聚不饱和脂肪酸（PUFA）水平0.5-5倍的方法，所述方法包括将抗病毒剂接种到培养基中的步骤， 在室温下培养培养2至5天，通过离心收获细胞以获得生物质，将生物质储存在约10℃，持续时间为12至48小时，并从储存的生物质获得增加的PUFA。

    Biological carriers for the delivery of proteins or peptides
    7.
    发明申请
    Biological carriers for the delivery of proteins or peptides 有权
    用于递送蛋白质或肽的生物载体

    公开(公告)号：US20040092467A1

    公开(公告)日：2004-05-13

    申请号：US10293867

    申请日：2002-11-12

    发明人： Kwang-Poo Chang ,  Shigeru Sassa ,  Jerome F. Sah ,  Hiroya Ito ,  Bala Krishna Kolli ,  Daniel A. Peterson

    IPC分类号： A61K048/00 ,  C12N001/10

    CPC分类号： C12N15/87 ,  A61K47/60 ,  A61K2039/523 ,  Y02A50/41

    摘要： Methods for delivering potentially therapeutic or prophylactic protein and peptide agents to mammalian cells are provided. The agents are delivered by mutant trypanosomatid protozoa that have been genetically manipulated to code for such protein or peptide agents. The mutant protozoa additionally lack certain enzymes within the heme biosynthetic pathway, making the mutants susceptible to porphyria and eventual lysis.

    摘要翻译： 提供了向哺乳动物细胞递送潜在的治疗性或预防性蛋白质和肽剂的方法。 这些试剂通过遗传操作的突变体锥虫病原生动物递送以编码这种蛋白质或肽剂。 突变体原生动物在血红素生物合成途径中另外缺乏某些酶，使突变体易于卟啉症和最终的裂解。

    Efficient expression of Plasmodium apical membrane antigen in yeast cells
    8.
    发明申请
    Efficient expression of Plasmodium apical membrane antigen in yeast cells 有权
    酵母细胞中疟原虫顶端膜抗原的高效表达

    公开(公告)号：US20040091971A1

    公开(公告)日：2004-05-13

    申请号：US10615615

    申请日：2003-07-08

    发明人： Clemens Hendricus, M. Kocken ,  Alan William Thomas ,  Michael John Blackman ,  Chrislaine Withers-Martinez ,  Anthony Arthur Holder

    IPC分类号： C12P021/02 ,  C12N001/18 ,  C12N001/10 ,  C12N015/74

    CPC分类号： C12N15/815 ,  A61K39/00 ,  C07K14/445 ,  Y02A50/412

    摘要： A method of efficiently expressing Plasmodium AMA-1 ectodomain or a functional part, derivative and/or analogue thereof in a eukaryotic expression system. Preferably, the Plasmodium AMA-1 ectodomain is Pf AMA-1 ectodomain. This protein may be expressed in yeast, such as Pichia pastoris. Efficient expression is possible using a method for producing mRNA encoding said Plasmodium AMA-1 ectodomain in a yeast cell, comprising providing the yeast cell with a nucleic acid encoding Plasmodium AMA-1 ectodomain, the nucleic acid being modified to utilize the yeast's codon usage. Preferably, at least one putative yeast polyadenylation consensus sequence in the nucleic acid has been modified. More preferably, also at least one site in the protein that is generally glycosylated by eukaryotic expression systems, has been removed.

    摘要翻译： 在真核表达系统中有效表达疟原虫AMA-1胞外域或其功能部分，衍生物和/或类似物的方法。 优选地，疟原虫AMA-1胞外域是Pf AMA-1胞外域。 该蛋白质可以在酵母中表达，如巴斯德毕赤酵母。 可以使用在酵母细胞中产生编码所述疟原虫AMA-1胞外域的mRNA的方法进行有效的表达，包括向酵母细胞提供编码疟原虫AMA-1胞外域的核酸，所述核酸被修饰以利用酵母的密码子使用。 优选地，已经修饰了核酸中至少一种推定的酵母多聚腺苷酸化共有序列。 更优选地，还通过真核表达系统通常糖基化的蛋白质中的至少一个位点被去除。

    Toxin genes from the bacteria xenorhabdus nematophilus and photorhabdus luminescens
    9.
    发明申请
    Toxin genes from the bacteria xenorhabdus nematophilus and photorhabdus luminescens 失效
    来自细菌杀线虫的嗜血杆菌和光致瘤细菌的毒素基因

    公开(公告)号：US20040055036A1

    公开(公告)日：2004-03-18

    申请号：US10617962

    申请日：2003-07-14

    申请人： COMMONWEALTH SCIENTIFIC AND INDUSTRIAL ORGANISATION

    发明人： Peter David East

    IPC分类号： A01H001/00 ,  C12N015/82 ,  C12N001/18 ,  C07H021/04 ,  C12N001/21 ,  C12N001/10

    CPC分类号： A01N63/02 ,  A61K38/00 ,  C07K14/24 ,  C12N15/8286 ,  Y02A40/162

    摘要： The invention relates to the identification and isolation of polynucleotide molecules encoding a new class of protein insecticidal toxins which are produced by bacteria from the genera Xenorhabdus and Photorhabdus. The polynucleotide molecules may be incorporated into, for example, insect-specific viruses (including entomopox and nuclear polyhedrosis viruses), bacteria (including Gracilicutes, Firmicutes, Tenericutes and Mendosicutes), protozoa, yeast and plants for control of pest insects.

    摘要翻译： 本发明涉及鉴定和分离编码新品种的杀虫毒素的多核苷酸分子，其由来自Xenorhabdus和Photorhabdus的细菌产生。 多核苷酸分子可以并入例如昆虫特异性病毒（包括昆虫和核多角体病毒），细菌（包括Gracilicutes，Firmicutes，Tenericutes和Mendosicutes），原生动物，酵母和植物中以控制害虫。