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    Use of POX4 promoter to increase gene expression in Candida tropicalis
    1.
    发明申请
    Use of POX4 promoter to increase gene expression in Candida tropicalis 审中-公开
    使用POX4启动子增加热带假丝酵母中的基因表达

    公开(公告)号：US20040265980A1

    公开(公告)日：2004-12-30

    申请号：US10629266

    申请日：2003-07-29

    发明人： Yeyan Zhang ,  C. Ron Wilson ,  David L. Craft ,  L. Dudley Eirich ,  Robert W. Frayer

    IPC分类号： C12P007/64 ,  C12P007/40 ,  C12N001/18 ,  C12N015/74

    CPC分类号： C12N15/81 ,  C12P7/44

    摘要： The present invention provides methods of converting or increasing conversion of a fatty acid to its corresponding dicarboxylic acid. The methods comprise isolating a promoter from a yeast gene which gene is induced when the yeast is grown on a fatty acid or alkane substrate, and operably linking the promoter to a gene involved in dicarboxylic acid production to form an expression vector. Yeast cells are subsequently transformed with such an expression vector and cultured in a media containing an organic substrate biooxidizable to a mono- or polycarboxylic acid, and resultant yeast cells convert or increase conversion of fatty acids to their corresponding dicarboxylic acids. Examples of promoters that may be used in the methods of the present invention include those from C. tropicalis catalase, citrate synthase, 3-ketoacyl-CoA thiolase A, citrate synthase, O-acetylhomserine sulphydrylase, protease, carnitine O-acetyltransferase, hydratase-dehydrogenase, and epimerase genes. A preferred promoter for use in a subject expression vector is the POX4 gene promoter. Examples of genes involved in dicarboxylic acid production include members of an null-hydroxylase complex such as e.g., CYP, NCP, or CYTb5 genes. Host cells comprising such expression vectors are also provided. Preferred host cells are include Yarrowia, Candida, Bebaromyces, Saccharomyces, Schizosaccharomyces, and Pichia.

    Method for preparing free polyunsaturated fatty acids and their oxidation metabolites
    2.
    发明申请
    Method for preparing free polyunsaturated fatty acids and their oxidation metabolites 失效
    制备游离多不饱和脂肪酸及其氧化代谢物的方法

    公开(公告)号：US20040209334A1

    公开(公告)日：2004-10-21

    申请号：US10630359

    申请日：2003-07-30

    发明人： Kamal Bouarab ,  Jean-Pierre Salaun ,  Jean-Claude Yvin ,  Philippe Potin

    IPC分类号： C11B001/00 ,  C12P007/64

    CPC分类号： C12P7/6427 ,  C12P7/6472

    摘要： The invention relates to a method for preparing free polyunsaturated fatty acids and their oxidation metabolites, wherein, successively, the production of polyunsaturated fatty acids and of their oxidation metabolites is stimulated, in a red alga, by the action of an elicitor which is peptide, lipid or saccharide in nature, and then the polyunsaturated fatty acids produced and also their oxidation metabolites are extracted.

    摘要翻译： 本发明涉及一种制备游离多不饱和脂肪酸及其氧化代谢物的方法，其中依次通过作为肽的激发剂的作用在红藻中刺激多不饱和脂肪酸及其氧化代谢产物的产生， 脂质或糖类，然后提取产生的多不饱和脂肪酸及其氧化代谢物。

    Fatty acyl-CoA: fatty alcohol acyltransferases
    4.
    发明申请
    Fatty acyl-CoA: fatty alcohol acyltransferases 有权
    脂肪酰基辅酶A：脂肪醇酰基转移酶

    公开(公告)号：US20030228668A1

    公开(公告)日：2003-12-11

    申请号：US10418146

    申请日：2003-04-18

    发明人： Kathryn Dennis Lardizabal ,  James George Metz ,  Michael W. Lassner

    IPC分类号： C12P007/64 ,  C12N009/10 ,  C07H021/04 ,  C12P021/02 ,  C12N001/21 ,  C12N005/04 ,  C12N005/06 ,  A23D009/00

    CPC分类号： C12P7/6463 ,  C12N9/1029 ,  C12N15/8247

    摘要： By this invention, nucleic acid sequences encoding for fatty acyl-CoA:fatty alcohol acyltransferase (wax synthase) are provided, wherein said wax synthase is active in the formation of a wax ester from fatty alcohol and fatty acyl-CoA substrates. Of special interest is are nucleic acid sequences obtainable from a jojoba embryo wax synthase having an apparent molecular mass of approximately 33 kD. Also considered are amino acid and nucleic acid sequences obtainable from wax synthase proteins and the use of such sequences to provide transgenic host cells capable of producing wax esters.

    摘要翻译： 通过本发明，提供了编码脂肪酰基-CoA：脂肪醇酰基转移酶（蜡合酶）的核酸序列，其中所述蜡合酶在脂肪醇和脂肪酰辅酶A底物形成蜡酯中是有活性的。 特别感兴趣的是可从表观分子量为约33kD的霍霍巴胚蜡合酶获得的核酸序列。 还考虑了可从蜡合酶蛋白获得的氨基酸和核酸序列，以及使用这些序列提供能够生产蜡酯的转基因宿主细胞。

    Process for producing sterol or stanol esters by enzymatic transesterification in solvent and water free media
    6.
    发明申请
    Process for producing sterol or stanol esters by enzymatic transesterification in solvent and water free media 失效
    通过在溶剂和无水介质中酶促酯交换生产甾醇或甾烷醇酯的方法

    公开(公告)号：US20030092096A1

    公开(公告)日：2003-05-15

    申请号：US10205964

    申请日：2002-10-01

    发明人： Miguel Angel Fuenzalida Diaz ,  Alejandro Markovits Rojas ,  Endre Markovits Schersl ,  Irene Martinez Basterrechea

    IPC分类号： C12P033/00 ,  C12P007/64 ,  C07J001/00

    CPC分类号： C12P7/6436 ,  C07J1/00 ,  C12N9/20 ,  C12P7/62 ,  C12P33/00

    摘要： A process for producing steryl or stanyl esters, comprises the steps of: a) forming a reacting mixture by contacting a lipase with a reactant mixture wherein the reactant mixture comprise sterols or stanols and one or more esters selected from the group consisting of esters of a fatty acid with a short chain aliphatic alcohol; b) separating the lipase from the reacting mixture to form a reacted mixture; and c) separating steryl or stanyl esters from the reacted mixture. The reaction is carried out in stirred reactors at pressures below atmospheric pressure and temperatures from 30 to 90null C. The separation of esters from the reacted mixtures is carried out by distillation at low pressures. A process for esterifying sterols or stanols comprise forming a reacting mixture by contacting a lipase with a reactant mixture wherein the reactant mixture comprise sterols or stanols and one or more esters selected from the group consisting of esters of a fatty acid with a short chain aliphatic alcohol. The reaction of esterification is carried out in stirred reactors at pressures below atmospheric pressure and temperatures from 30 to 90null C.

    摘要翻译： 一种生产甾体或萜烯酯的方法，包括以下步骤：a）通过使脂肪酶与反应物混合物接触来形成反应混合物，其中反应物混合物包含甾醇或甾烷醇和一种或多种选自以下的酯：酯 脂肪酸与短链脂肪醇; b）将脂肪酶与反应混合物分离以形成反应混合物; 和c）从反应的混合物中分离出甾或酯。 该反应在搅拌反应器中在低于大气压和30至90℃的温度下进行。酯与反应的混合物的分离通过在低压下蒸馏进行。 用于酯化甾醇或甾烷醇的方法包括通过使脂肪酶与反应物混合物接触来形成反应混合物，其中反应物混合物包含甾醇或甾烷醇和一种或多种选自脂肪酸的酯与短链脂族醇的酯 。 酯化反应在搅拌反应器中在低于大气压和30至90℃的温度下进行。

    Fatty acid epoxygenase genes from plants and uses therefor in modifying fatty acid metabolism
    8.
    发明申请
    Fatty acid epoxygenase genes from plants and uses therefor in modifying fatty acid metabolism 有权
    来自植物的脂肪酸环氧化酶基因，用于修饰脂肪酸代谢

    公开(公告)号：US20020166144A1

    公开(公告)日：2002-11-07

    申请号：US09981124

    申请日：2001-10-17

    发明人： Allan Green ,  Surinder Singh ,  Marit Lenman ,  Sten Stymne

    IPC分类号： C12N015/82 ,  A01H005/00 ,  C12N015/87 ,  C12P007/64 ,  C12N015/29 ,  C12N009/02

    CPC分类号： C12N9/0071 ,  C12N9/0004 ,  C12N15/8247 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6472

    摘要： The present invention relates generally to novel genetic sequences that encode fatty acid epoxygenase enzymes, in particular fatty acid null12-epoxygenase enzymes from plants that are mixed function monooxygenase enzymes. More particularly, the present invention exemplifies cDNA sequences from Crepis spp. and Vernonia galamensis that encode fatty acid null12-epoxygenases. The genetic sequences of the present invention provide the means by which fatty acid metabolism may be altered or manipulated in organisms, such as, for example, yeasts, moulds, bacteria, insects, birds, mammals and plants, and more particularly in plants. The invention also extends to genetically modified oil-accumulating organisms transformed with the subject genetic sequences and to the oils derived therefrom. The oils thus produced provide the means for the cost-effective raw materials for use in the efficient production of coatings, resins, glues, plastics, surfactants and lubricants.

    摘要翻译： 本发明一般涉及编码脂肪酸环氧化酶的新型遗传序列，特别是来自作为混合功能单加氧酶的植物的脂肪酸DELTA12-环氧化酶。 更具体地，本发明举例说明了来自Crepis spp的cDNA序列。 和Vernonia galamensis，编码脂肪酸DELTA12-环氧化酶。 本发明的遗传序列提供了在生物体例如酵母，霉菌，细菌，昆虫，鸟类，哺乳动物和植物中，更具体地在植物中可以改变或操纵脂肪酸代谢的方法。 本发明还延伸到用受试者遗传序列转化的转基因油积聚生物和由其衍生的油。 因此生产的油为用于有效生产涂料，树脂，胶水，塑料，表面活性剂和润滑剂的成本效益好的原材料提供了手段。

    Multi-gene expression constructs containing modified inteins
    9.
    发明申请
    Multi-gene expression constructs containing modified inteins 失效
    含有修饰内含肽的多基因表达构建体

    公开(公告)号：US20020129400A1

    公开(公告)日：2002-09-12

    申请号：US09779957

    申请日：2001-02-09

    发明人： Kristi D. Snell

    IPC分类号： C12N015/82 ,  A01H005/00 ,  C12P007/64 ,  C12N005/04

    CPC分类号： C12N15/8216 ,  C12P7/625

    摘要： Methods and constructs for the introduction of multiple genes into plants using a single transformation event are described. Constructs contain a single 5null promoter operably linked to DNA encoding a modified intein splicing unit. The splicing unit is expressed as a polyprotein and consists of a first protein fused to an intein fused to a second protein. The splicing unit has been engineered to promote excision of all non-essential components in the polyprotein but prevent the ligation reactions normally associated with protein splicing. Additional genetic elements encoding inteins and additional proteins can be fused in frame to the 5null-terminus of the coding region for the second protein to form a construct for expression of more than two proteins. A single 3null termination sequence, such as a polyadenylation sequence when the construct is to be expressed in eucaryotic cells, follows the last coding sequence. These methods and constructs are particularly useful for creating plants with stacked input traits, illustrated by glyphosate tolerant plants producing BT toxin, and/or value added products, illustrated by the production of polyhydroxyalkanoates in plants.

    摘要翻译： 描述了使用单一转化事件将多个基因引入植物的方法和构建体。 构建体包含与编码修饰的内含肽剪接单元的DNA可操作地连接的单个5'启动子。 剪接单元表示为多聚蛋白，由融合至第二蛋白质的内含肽融合的第一个蛋白质组成。 剪接单元已被设计用于促进多蛋白中所有非必需组分的切除，但是防止通常与蛋白质剪接相关的连接反应。 编码内含肽和其他蛋白质的其他遗传元件可以与第二个蛋白质的编码区的5'端框架融合，形成表达两种以上蛋白质的构建体。 当构建体在真核细胞中表达时，单个3'终止序列，例如聚腺苷酸化序列，遵循最后的编码序列。 这些方法和结构对于产生具有堆叠输入性状的植物特别有用，由产生BT毒素的草甘膦耐受植物和/或增值产物说明，其通过在植物中产生聚羟基链烷酸酯来说明。