公开(公告)号：US20040203120A1

公开(公告)日：2004-10-14

申请号：US10393366

申请日：2003-03-21

申请人： Council of Scientific and Industrial Research

发明人： Seshagiri Raghu Kumar ,  Ruchi Jain

IPC分类号： C12P007/64 ,  C12N001/10

CPC分类号： C12P7/6427 ,  C12P7/6472

摘要： The present invention relates to a method of enhancing the levels of Poly-unsaturated fatty acids (PUFA) by 0.5 to 5 times in protests thraustochytrid protests by storing the cells under cold conditions, said method comprising steps of inoculating the protests into a culture medium, growing the culture for 2 to 5 days at room temperature, harvesting the cells by centrifugation to obtain biomass, storing the biomass at about 10null C. for time duration ranging between 12 to 48 hours, and obtaining increased PUFA from the stored biomass.

摘要翻译： 本发明涉及一种通过在冷条件下储存细胞来提高抗坏血酸破坏性抗原活动中多聚不饱和脂肪酸（PUFA）水平0.5-5倍的方法，所述方法包括将抗病毒剂接种到培养基中的步骤， 在室温下培养培养2至5天，通过离心收获细胞以获得生物质，将生物质储存在约10℃，持续时间为12至48小时，并从储存的生物质获得增加的PUFA。