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    Utilization of Cell Forcibly Expressing Toll-Like Receptor
    1.
    发明申请
    Utilization of Cell Forcibly Expressing Toll-Like Receptor 有权
    细胞强迫表达Toll样受体的应用

    公开(公告)号：US20070298449A1

    公开(公告)日：2007-12-27

    申请号：US10561022

    申请日：2004-03-05

    申请人： Tadao Saito ,  Takeshi Shimosato ,  Haruki Kitazawa

    发明人： Tadao Saito ,  Takeshi Shimosato ,  Haruki Kitazawa

    IPC分类号： C12N15/87 ,  C12N5/06 ,  C12Q1/02

    CPC分类号： G01N33/502 ,  C07K14/705 ,  G01N33/5008 ,  G01N33/5044 ,  Y10S514/885 ,  Y10S530/837 ,  Y10S530/844

    摘要： Cells with forced expression of swine Toll-like receptor 9 (TLR9) are prepared by cloning a TLR9 gene from swine intestinal Peyer's patches. Functional analysis on CpG DNAs using the above cells revealed that swine TLR9 shows a higher recognition ability for a human CpG DNA motif (CpG2006) than for a mouse-specific CpG DNA motif (CpG1826). When the mRNA expression levels in various tissues are compared by the real-time PCR method, it is found out that the mRNA is expressed in Peyer's patches and mesenteric lymph nodes, which play important roles in the intestinal tract immune system, at a level thrice as much as in spleen or more. Thus, the cells that are forced to express an intestinal tract tissue-expressed TLR (for example, TLR9) can be used to identify samples capable of activating the intestinal tract immune system.

    摘要翻译： 通过克隆来自猪肠粘膜斑块的TLR9基因制备猪Toll样受体9（TLR9）强制表达的细胞。 使用上述细胞对CpG DNA的功能分析表明，猪TLR9对人CpG DNA基序（CpG2006）的识别能力高于小鼠特异性CpG DNA基序（CpG1826）。 当通过实时PCR方法比较各种组织中的mRNA表达水平时，发现mRNA在肠粘膜和肠系膜淋巴结中表达，其在肠道免疫系统中起重要作用，一级三次 多于脾或更多。 因此，被迫表达肠道组织表达的TLR（例如，TL​​R9）的细胞可用于鉴定能够激活肠道免疫系统的样品。

    Methods and compositions for the detection of colon cancers
    2.
    发明授权
    Methods and compositions for the detection of colon cancers 失效

    公开(公告)号：US5830677A

    公开(公告)日：1998-11-03

    申请号：US448048

    申请日：1995-05-23

    申请人： Ying-Jye Wu ,  Susan K. Keesee

    发明人： Ying-Jye Wu ,  Susan K. Keesee

    IPC分类号： C07K14/47 ,  C07K16/30 ,  G01N33/574 ,  G01N33/68 ,  G01N33/53

    CPC分类号： C07K14/47 ,  C07K16/3046 ,  G01N33/57419 ,  G01N33/57446 ,  G01N33/6893 ,  Y10S436/813 ,  Y10S530/828 ,  Y10S530/844

    摘要： The invention provides methods and compositions for identifying colon cancers in an individual. In one embodiment of the invention, a colon adenocarcinoma-associated protein may be detected in a tissue or body fluid sample of an individual, to provide an indication of the presence of an adenocarcinoma of the colon in the individual. The target adenocarcinoma-associated protein, may be detected, e.g., by reacting the sample with a labeled binding moiety, such as a labeled antibody, capable of specifically binding the protein. In another embodiment, the protein may be detected by isolating one or more colon adenocarcinoma-associated proteins from the sample, separating the proteins by two dimensional gel electrophoresis, and comparing the gel electrophoresis pattern with a standard. The invention provides a wide range of assay methods and compositions which may be used for detecting colon tumors in an individual rapidly and reproducibly, optimally at early stages of the disease.

    Protein cross-linked with phosphonitrilic halide
    3.
    发明授权
    Protein cross-linked with phosphonitrilic halide 失效
    蛋白质与磷酸盐交联

    公开(公告)号：US3674509A

    公开(公告)日：1972-07-04

    申请号：US3674509D

    申请日：1970-10-27

    申请人： MONSANTO CO

    发明人： MILLER ROBERT E

    IPC分类号： A23K1/16 ,  A23J3/00 ,  A23K1/18

    CPC分类号： A23K20/147 ,  Y10S530/844

    摘要： IMPROVED PROTEIN FEED MATERIAL FOR RUMINANTS WHICH IS RESISTANT TO DIGESTIVE BREAKDOWN IN THE RUMEN BUT NOT IN THE ABOMASUM AND/OR INTESTINES WHICH COMPRISES THE REACTION PRODUCT OF A PROTEIN-CONTAINING FEED MATERIAL AND A PHOSPHONITRILIC HALIDE. EXEMPLARY OF SUCH PHOSPHONITRILLIC HALIDES IS PHOSPHONITRILLIC CHLORIDE TRIMER.

    Methods and compositions for the detection of colon cancers
    7.
    发明授权
    Methods and compositions for the detection of colon cancers 失效
    用于检测结肠癌的方法和组合物

    公开(公告)号：US5547928A

    公开(公告)日：1996-08-20

    申请号：US168993

    申请日：1993-12-17

    申请人： Ying-Jye Wu ,  Susan K. Keesee

    发明人： Ying-Jye Wu ,  Susan K. Keesee

    IPC分类号： C07K14/47 ,  C07K16/30 ,  G01N33/574 ,  G01N33/68 ,  A61K38/17 ,  A61K35/38 ,  C07K1/28 ,  C07K14/435

    CPC分类号： C07K14/47 ,  C07K16/3046 ,  G01N33/57419 ,  G01N33/57446 ,  G01N33/6893 ,  Y10S436/813 ,  Y10S530/828 ,  Y10S530/844

    摘要： The invention provides methods and compositions for identifying colon cancers in an individual. In one embodiment of the invention, a colon adenocarcinoma-associated protein may be detected in a tissue or body fluid sample of an individual, to provide an indication of the presence of an adenocarcinoma of the colon in the individual. The target adenocarcinoma-associated protein, may be detected, e.g., by reacting the sample with a labeled binding moiety, such as a labeled antibody, capable of specifically binding the protein. In another embodiment, the protein may be detected by isolating one or more colon adenocarcinoma-associated proteins from the sample, separating the proteins by two dimensional gel electrophoresis, and comparing the gel electrophoresis pattern with a standard. The invention provides a wide range of assay methods and compositions which may be used for detecting colon tumors in an individual rapidly and reproducibly, optimally at early stages of the disease.

    摘要翻译： 本发明提供用于鉴定个体中结肠癌的方法和组合物。 在本发明的一个实施方案中，可以在个体的组织或体液样品中检测结肠腺癌相关蛋白，以提供个体中结肠腺癌的存在的指示。 目标腺癌相关蛋白可以例如通过使样品与能够特异性结合蛋白质的标记结合部分，例如标记的抗体反应来检测。 在另一个实施方案中，可以通过从样品中分离一种或多种结肠腺癌相关蛋白来检测蛋白质，通过二维凝胶电泳分离蛋白质，并将凝胶电泳图谱与标准物进行比较。 本发明提供了广泛的测定方法和组合物，其可用于在疾病的早期阶段快速且可重复地，最佳地检测个体中的结肠肿瘤。

    Separation of secretin and cholecystokinin-pancreozymin from mixture thereof
    9.
    发明授权
    Separation of secretin and cholecystokinin-pancreozymin from mixture thereof 失效
    从其混合物中分离分离蛋白和选择性蛋白 - 胰蛋白酶

    公开(公告)号：US3847891A

    公开(公告)日：1974-11-12

    申请号：US35224073

    申请日：1973-04-18

    申请人： EISAI CO LTD

    发明人： TACHIBANA S

    IPC分类号： A61K38/00 ,  C07K14/595 ,  A61K27/00 ,  C07C103/52

    CPC分类号： C07K14/595 ,  A61K38/00 ,  Y10S530/844

    摘要： 1. A PROCESS FOR THE SEPARATION OF SECRETIN AND CHOLECYSTOKININ-PANCREOZYMIN FROM A MIXTURE THEREOF, COMPRISING (A) CONTACTING THE MIXTURE IN AQUEOUS SOLUTION AT A PH RANGING FROM ABOUT 9 TO ABOUT 12, WITH AN ANIONEXCHANGE RESIN TO CAUSE SELECTIVE ADSORPTION OF CHOLECYSTOKININ-PANCREOZYMIN ON SAID RESIN AND RESERVING THE REMAINING AQUEOUS SOLUTION, (B) ELUTING THE CHLOECYSTOKININ-PANCREOZYMIN FROM SAID RESIN BY CONTACTING IT WITH AN AQUEOUS ELUTING MEDIUM AT PH LOWER THAN 9: AND (C9 RECOVERING THE CHOLECYSTOKININ-PANCREZYOIN FROM THE ELUATE OF STEP (B) AND RECOVERING THE SECRETIN FROM THE AQUEOUS SOLUTION OBTAINED FROM STEP 8A), WHICH SOLUTION CONTAINS THE SECRETION NOT ADSORBED BY SAID RESIN.