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12 条记录 (耗时 0.011 秒)

    Immunoassay for AH receptor transformed by dioxin-like compounds
    1.
    发明授权
    Immunoassay for AH receptor transformed by dioxin-like compounds 失效
    由二恶英样化合物转化的AH受体的免疫测定

    公开(公告)号：US5529899A

    公开(公告)日：1996-06-25

    申请号：US98142

    申请日：1993-07-27

    申请人： Geoffrey D. Wheelock ,  John G. Babish

    发明人： Geoffrey D. Wheelock ,  John G. Babish

    IPC分类号： G01N33/15 ,  G01N33/53 ,  G01N33/566 ,  G01N33/567

    CPC分类号： G01N33/5308 ,  G01N33/566 ,  G01N33/567 ,  G01N2430/40 ,  Y10S435/97 ,  Y10S435/975 ,  Y10S436/807 ,  Y10S436/809

    摘要： The present invention is directed to a method of detecting dioxin-like compounds in a test sample. The test sample is contacted with a heteromer formed from a plurality of proteins, one of which is an inactive Ah receptor. If dioxin-like compounds are present in the test sample, they will bind to the Ah receptor causing it to dissociate from the heteromer as a complex containing active Ah receptor bound to a dioxin-like compound ligand. The presence of the complex is then detected. The process of the present invention can be practiced utilizing solid phase capture, competitive, and sandwich immunoassay test kit formats.

    摘要翻译： 本发明涉及一种在测试样品中检测二恶英样化合物的方法。 将测试样品与由多种蛋白质形成的异源物接触，其中一种蛋白质是无活性的Ah受体。 如果二恶英样化合物存在于测试样品中，则它们将与Ah受体结合，导致其与作为与二恶英样化合物配体结合的活性Ah受体的复合物从异聚体解离。 然后检测复合物的存在。 本发明的方法可以利用固相捕获，竞争性和夹心免疫测定试剂盒形式来实施。

    Indirect immunoassay for dioxinlike compounds
    3.
    发明授权
    Indirect immunoassay for dioxinlike compounds 失效
    二恶英类化合物的间接免疫测定

    公开(公告)号：US5496703A

    公开(公告)日：1996-03-05

    申请号：US132164

    申请日：1993-10-05

    申请人： John G. Babish ,  Sakina El-Tom ,  Xinfang Ma ,  Goeffrey Wheelock

    发明人： John G. Babish ,  Sakina El-Tom ,  Xinfang Ma ,  Goeffrey Wheelock

    IPC分类号： G01N33/15 ,  G01N33/53 ,  G01N33/566 ,  G01N33/567 ,  C12Q1/02

    CPC分类号： G01N33/5308 ,  G01N33/566 ,  G01N33/567 ,  G01N2430/40 ,  Y10S435/97 ,  Y10S435/975 ,  Y10S436/807 ,  Y10S436/809

    摘要： This invention relates to the determination of in vivo exposure to dioxinlike compounds and the in vitro quantitation of dioxinlike compounds based on protein tyrosine phosphorylation as mediated by the Ah receptor. The extent of protein tyrosine phosphorylation is determined by monoclonal antibodies to protein tyrosine phosphate. The amount and nature of protein tyrosylphosphate is quantitatively related to the in vivo or in vitro concentration of dioxin to which animals or cells were exposed. This method permits detection of dioxinlike compounds at concentrations in the parts per trillion and parts per billion range.

    摘要翻译： 本发明涉及二恶英类化合物的体内暴露的测定以及基于由Ah受体介导的蛋白质酪氨酸磷酸化的二恶英样化合物的体外定量。 蛋白质酪氨酸磷酸化的程度由磷酸蛋白酪氨酸的单克隆抗体决定。 蛋白酪蛋白磷酸盐的量和性质与动物或细胞暴露于二恶英的体内或体外浓度定量相关。 该方法允许以每万亿分之几和十亿分之几的浓度检测二恶英类化合物。

    Method and system of detecting dioxin-like compounds
    4.
    发明授权
    Method and system of detecting dioxin-like compounds 有权
    检测二恶英类化合物的方法和系统

    公开(公告)号：US08877434B2

    公开(公告)日：2014-11-04

    申请号：US13451498

    申请日：2012-04-19

    申请人： Hsinyu Lee

    发明人： Hsinyu Lee

    IPC分类号： C12Q1/00 ,  C12Q1/66

    CPC分类号： G01N21/6428 ,  G01N21/763 ,  G01N33/5308 ,  G01N33/542 ,  G01N2430/40

    摘要： The invention relates to a method or a cell free system of detecting dioxin-like compounds in a test sample using a whole cell lysate derived from a cell transfected to express a fusion protein comprising an AHR fused to a reporter peptide. The method in combination with bioluminescence resonance energy transfer (BRET) technique is also provided so as to improve the sensitivity.

    摘要翻译： 本发明涉及使用衍生自转染细胞的全细胞裂解物来检测测试样品中的二恶英样化合物的方法或无细胞系统，以表达包含与报道肽融合的AHR的融合蛋白。 还提供了结合生物发光共振能量转移（BRET）技术的方法，以提高灵敏度。

    METHOD AND SYSTEM OF DETECTING DIOXIN-LIKE COMPOUNDS
    5.
    发明申请
    METHOD AND SYSTEM OF DETECTING DIOXIN-LIKE COMPOUNDS 有权
    检测二氧化物化合物的方法和系统

    公开(公告)号：US20130280741A1

    公开(公告)日：2013-10-24

    申请号：US13451498

    申请日：2012-04-19

    申请人： Hsinyu Lee

    发明人： Hsinyu Lee

    IPC分类号： G01N21/76

    CPC分类号： G01N21/6428 ,  G01N21/763 ,  G01N33/5308 ,  G01N33/542 ,  G01N2430/40

    摘要： The invention relates to a method or a cell free system of detecting dioxin-like compounds in a test sample using a whole cell lysate derived from a cell transfected to express a fusion protein comprising an AHR fused to a reporter peptide. The method in combination with bioluminescence resonance energy transfer (BRET) technique is also provided so as to improve the sensitivity.

    摘要翻译： 本发明涉及使用衍生自转染细胞的全细胞裂解物来检测测试样品中的二恶英样化合物的方法或无细胞系统，以表达包含与报道肽融合的AHR的融合蛋白。 还提供了结合生物发光共振能量转移（BRET）技术的方法，以提高灵敏度。

    Dioxin derivatives and method of measurement therewith
    6.
    发明申请
    Dioxin derivatives and method of measurement therewith 审中-公开
    二恶英衍生物及其测量方法

    公开(公告)号：US20030108955A1

    公开(公告)日：2003-06-12

    申请号：US10203628

    申请日：2002-11-01

    发明人： Noboru Yanaihara ,  Tsukasa Kodaira

    IPC分类号： G01N033/53 ,  C07D495/04

    CPC分类号： C07D319/24 ,  G01N33/5308 ,  G01N33/531 ,  G01N2430/40

    摘要： The invention provides a highly sensitive measurement and detection technique for dioxins, which has advantages inherent to immunoassay; and markers to be used in the technique. Disclosed are a biotinylated dioxin derivative of formula (1): 1 (wherein X represents a hydrogen atom or a chlorine atom; R1 represents a biotin residue; R2's, which may be identical to or different from one another, independently and individually represent an arginine residue or a lysine residue; n is an integer from 1 to 5 inclusive; and m is an integer from 1 to 3 inclusive), and an immunoassay method for dioxins characterized by using the derivatives as a marker.

    摘要翻译： 本发明提供了高灵敏度的二恶英测量和检测技术，其具有免疫测定固有的优点; 和用于该技术的标记物。 公开了式（1）的生物素化二恶英衍生物：其中X表示氢原子或氯原子; R1表示生物素残基; R2可彼此相同或不同，独立地和单独表示精氨酸残基 或赖氨酸残基; n为1〜5的整数，m为1〜3的整数），以及以该衍生物为标记的二恶英的免疫测定法。

    Method and Apparatus for Screening and Assaying Environmental Sample
    8.
    发明申请
    Method and Apparatus for Screening and Assaying Environmental Sample 审中-公开
    筛选和分析环境样品的方法和装置

    公开(公告)号：US20090292477A1

    公开(公告)日：2009-11-26

    申请号：US11922123

    申请日：2005-06-14

    申请人： Takashi Matsuki ,  Kazuyuki Sawadaishi ,  Chiwa Kataoka ,  Yoko Takagi

    发明人： Takashi Matsuki ,  Kazuyuki Sawadaishi ,  Chiwa Kataoka ,  Yoko Takagi

    IPC分类号： G01N33/48 ,  G06F19/00

    CPC分类号： G01N33/5308 ,  G01N21/05 ,  G01N21/6428 ,  G01N33/54366 ,  G01N2021/7763 ,  G01N2430/40

    摘要： A screening measurement technology enabling a prompt determination about whether or not further confirmation is required by another measuring method, for an environmental sample, such as a sample containing dioxins, is provided. A sample solution in which a known amount of antibodies are mixed, is poured into a measurement cell (101) where an antigen derivative (107) that acquires antibodies of a subject substance comprising an antigen, is arranged in a flow channel (106) for the sample solution; whether or not a measurement result is within a pre-determined range including a reference value of the subject substance is determined based upon time-series signals of the measurement result; and the measurement result is transmitted.

    摘要翻译： 提供了一种筛选测量技术，能够对环境样品（例如含有二恶英的样品）进行另一种测量方法是否需要进一步确认是否需要进一步确定。 将其中混合已知量的抗体的样品溶液倒入测量细胞（101）中，其中获得包含抗原的受试物质的抗体的抗原衍生物（107）被布置在流路（106）中，用于 样品溶液; 基于测量结果的时间序列信号确定测量结果是否在包括被摄体的参考值的预定范围内; 并传送测量结果。

    Method for preparing sample for analysis of dioxins
    9.
    发明申请
    Method for preparing sample for analysis of dioxins 审中-公开
    制备二恶英分析样品的方法

    公开(公告)号：US20090107213A1

    公开(公告)日：2009-04-30

    申请号：US11921502

    申请日：2006-03-30

    申请人： Katsuhisa Honda ,  Noriaki Hamada ,  Takashi Miyawaki

    发明人： Katsuhisa Honda ,  Noriaki Hamada ,  Takashi Miyawaki

    IPC分类号： G01N30/06

    CPC分类号： G01N30/06 ,  B01J20/08 ,  B01J20/103 ,  B01J20/20 ,  B01J20/28052 ,  B01J20/28057 ,  B01J20/2808 ,  B01J20/28083 ,  B01J2220/603 ,  G01N1/40 ,  G01N2030/062 ,  G01N2030/8804 ,  G01N2030/8809 ,  G01N2030/8854 ,  G01N2430/40

    摘要： An aliphatic hydrocarbon solvent solution obtained by extracting dioxins contained in a specimen such as soil with an aliphatic hydrocarbon solvent is supplied to an alumina layer 33 side of a sample-preparation column 30 in which the alumina layer 33 is placed on a carbon material layer 32. Upon flowing and passing of an aliphatic hydrocarbon solvent through the alumina layer 33 and the carbon material layer 32 in this order, the dioxins contained in the aliphatic hydrocarbon solvent solution are trapped in these layers. Then, one extracting solvent among toluene, a mixed solvent of toluene and an aliphatic hydrocarbon solvent, and a hydrophilic solvent capable of dissolving dioxins is supplied to the sample-preparation column 30 in the direction opposed to the passing direction of the aliphatic hydrocarbon solvent, the dioxins trapped in the carbon material layer 32 and the alumina layer 33 are extracted to obtain a sample for analysis of dioxins.

    摘要翻译： 将通过用脂肪族烃溶剂将土壤样品中含有的二恶英而得到的脂肪族烃类溶剂溶液供给到氧化铝层33的氧化铝层33，氧化铝层33放置在碳材料层32上 在使脂族烃溶剂依次流过氧化铝层33和碳材料层32时，脂肪族烃溶剂溶液中含有的二恶英被捕获在这些层中。 然后，将甲苯，甲苯和脂肪族烃溶剂的混合溶剂和能够溶解二恶英的亲水性溶剂中的一种萃取溶剂沿与脂族烃溶剂的通过方向相反的方向供给到样品制备塔30， 捕获在碳材料层32和氧化铝层33中的二氧diox类物质，得到二恶英分析用样品。