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    APPARATUS AND METHODS FOR FLUORESCENCE IMAGING USING RADIOFREQUENCY-MULTIPLEXED EXCITATION
    2.
    发明申请
    APPARATUS AND METHODS FOR FLUORESCENCE IMAGING USING RADIOFREQUENCY-MULTIPLEXED EXCITATION 有权
    使用放射性多重激发进行荧光成像的装置和方法

    公开(公告)号：US20160003741A1

    公开(公告)日：2016-01-07

    申请号：US14792282

    申请日：2015-07-06

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    发明人： Eric D. Diebold ,  Bahram Jalali ,  Brandon Buckley

    IPC分类号： G01N21/64 ,  G02B21/16

    CPC分类号： G01N21/6408 ,  G01N21/05 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2021/6415 ,  G01N2021/6419 ,  G01N2201/06113 ,  G02B21/0076 ,  G02B21/0084 ,  G02B21/16 ,  G02B27/1006 ,  G02F1/11

    摘要： Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

    摘要翻译： 使用射频复用激发进行荧光成像的装置和方法。 一个装置将激发激光束分成Mach-Zehnder干涉仪的两个臂。 第一光束中的光被声光偏转器偏移，声光偏转器由相位设计的射频梳驱动，旨在最小化峰值与平均功率比。 该RF梳产生具有一定范围的输出角和频移的多个偏转光束。 第二个波束使用声光移频器在频率上移动。 在第二分光器组合之后，使用传统的激光扫描显微镜透镜系统将两个光束聚焦到样品上的线上。 声光偏转器对整行像素的同时激励进行频率编码，这使得能够使用单个光电倍增管和数字相位相干信号恢复技术来检测和解复用荧光图像。

    SYSTEM AND METHOD FOR DENSE-STOCHASTIC-SAMPLING IMAGING
    3.
    发明申请
    SYSTEM AND METHOD FOR DENSE-STOCHASTIC-SAMPLING IMAGING 有权
    用于渗透 - 采样成像的系统和方法

    公开(公告)号：US20120268584A1

    公开(公告)日：2012-10-25

    申请号：US13535468

    申请日：2012-06-28

    申请人： GAUDENZ DANUSER ,  PAUL C. GOODWIN

    发明人： GAUDENZ DANUSER ,  PAUL C. GOODWIN

    IPC分类号： H04N7/18

    CPC分类号： G01N21/6428 ,  G01N21/636 ,  G01N21/6458 ,  G01N2021/6415 ,  G01N2021/6419 ,  G01N2201/126 ,  G02B21/365 ,  G02B21/367 ,  G06T3/4053

    摘要： Embodiments of the present invention are directed to imaging technologies, and, in particular, to an imaging system that detects relatively weak signals, over time, and that uses the detected signals to determine the positions of signal emitters. Particular embodiments of the present invention are directed to methods and systems for imaging fluorophore-labeled samples in order to produce images of the sample at resolutions significantly greater than the diffraction-limited resolution associated with optical microscopy. Embodiments of the present invention employ overlapping-emitter-image disambiguation to allow data to be collected from densely arranged emitters, which significantly decreases the data-collection time for producing intermediate images as well as the number of intermediate images needed to computationally construct high-resolution final images. Additional embodiments of the present invention employ hierarchical image-processing techniques to further resolve and interpret disambiguated images.

    摘要翻译： 本发明的实施例涉及成像技术，特别涉及一种随时间检测相对较弱信号的成像系统，并且使用所检测的信号来确定信号发射器的位置。 本发明的具体实施方案涉及用于成像荧光团标记的样品的方法和系统，以便以明显大于与光学显微镜相关联的衍射限制分辨率的分辨率产生样品的图像。 本发明的实施例采用重叠 - 发射 - 图像消歧以允许从密集布置的发射器收集数据，这显着减少用于产生中间图像的数据收集时间以及计算构建高分辨率所需的中间图像的数量 最终图像。 本发明的另外的实施例采用分层图像处理技术来进一步解析和解释消歧图像。

    MICROSCOPE
    4.
    发明申请
    MICROSCOPE 审中-公开
    显微镜

    公开(公告)号：US20100014156A1

    公开(公告)日：2010-01-21

    申请号：US12438994

    申请日：2007-08-08

    申请人： Yoshinori Iketaki

    发明人： Yoshinori Iketaki

    IPC分类号： G02B21/06

    CPC分类号： G02B21/16 ,  G01N21/6458 ,  G01N2021/6415 ,  G01N2201/06113 ,  G01N2201/104 ,  G01N2201/1053 ,  G02B5/20 ,  G02B5/3083 ,  G02B21/002 ,  G02B26/06 ,  G02B2207/113

    摘要： A microscope for observing a sample containing a substance having at least two excited quantum states includes a pump light source 21 for emitting pump light, an erase light source 22 for emitting erase light, a light combining section 23 to 26 for coaxially combining the pump light and the erase light, a light collecting section 62 for collecting the combined lights, a scanning section 44 and 45 for scanning the sample with the combined lights, a detecting section 50 for detecting photoresponsive signals generated from the sample, a wavelength selecting element 42 arranged in the light path of the combined lights and provided with an erase light selecting region having a high wavelength selectivity for the erase light and with a pump light selecting region having a high wavelength selectivity for the pump light, and a space modulating element 43 arranged in the light path of the combined lights for spatially modulating the erase light corresponding to the erase light selecting region of the wavelength selecting element.

    摘要翻译： 用于观察含有具有至少两个激发量子态的物质的样品的显微镜包括用于发射泵浦光的泵浦光源21，用于发射擦除光的擦除光源22，用于同时组合泵浦光的光合成部23至26 擦除光，用于收集组合光的光收集部分62，用于组合光扫描样本的扫描部分44和45;检测部分50，用于检测从样本产生的光响应信号;波长选择元件42，被布置 在组合光的光路中设置有对于擦除光具有高波长选择性的擦除光选择区域和对泵浦光具有高波长选择性的泵浦光选择区域，以及空间调制元件43，其布置在 用于空间调制对应于擦除光选择区域的擦除光的组合光的光路 的波长选择元件。

    Computer controlled fluorometer device and method of operating same
    5.
    发明授权
    Computer controlled fluorometer device and method of operating same 失效
    计算机控制的荧光计装置及其操作方法

    公开(公告)号：US4942303A

    公开(公告)日：1990-07-17

    申请号：US304273

    申请日：1989-01-31

    申请人： Zbigniew Kolber ,  Paul Falkowski

    发明人： Zbigniew Kolber ,  Paul Falkowski

    IPC分类号： G01N21/63 ,  G01N21/64

    CPC分类号： G01N21/645 ,  G01N21/6486 ,  G01N2021/635 ,  G01N2021/6415 ,  G01N2201/0218 ,  G01N2201/0696

    摘要： A computer controlled fluorometer device and method of operating same, said device being made to include a pump flash source and a probe flash source and one or more sample chambers in combination with a light condenser lens system and associated filters and reflectors and collimators, as well as signal conditioning and monitoring means and a programmable computer means and a software programmable source of background irradiance that is operable according to the method of the invention to rapidly, efficiently and accurately measure photosynthetic activity by precisely monitoring and recording changes in fluorescence yield produced by a controlled series of predetermined cycles of probe and pump flashes from the respective probe and pump sources that are controlled by the computer means.

    摘要翻译： 计算机控制的荧光计装置及其操作方法，所述装置被制成包括泵闪光源和探针闪光源以及与光聚光透镜系统以及相关联的滤光器和反射器和准直器组合的一个或多个样本室 作为信号调节和监测装置以及根据本发明的方法可操作的可编程计算机装置和软件可编程的背景辐照源，通过精确地监测和记录由a产生的荧光产率的变化来快速，有效和准确地测量光合作用活性 探针和泵的预定循环的受控系列从由计算机装置控制的相应探针和泵浦源闪烁。

    TIME MEASURING DEVICE, FLUORESCENCE LIFETIME MEASURING DEVICE, AND TIME MEASURING METHOD
    6.
    发明公开
    TIME MEASURING DEVICE, FLUORESCENCE LIFETIME MEASURING DEVICE, AND TIME MEASURING METHOD 审中-公开

    公开(公告)号：US20230288336A1

    公开(公告)日：2023-09-14

    申请号：US18017698

    申请日：2021-05-13

    申请人： HAMAMATSU PHOTONICS K.K.

    发明人： Fuminori NIIKURA ,  Ayumu YOSHIMI ,  Ken KITAZAWA ,  Takayuki INOUE

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6408 ,  G01N2201/123 ,  G01N2021/6415

    摘要： A time measuring apparatus 10 includes a digital counter 20 that outputs a count signal in response to a clock signal, a plurality of TAC circuits 12 (TAC circuits 12a to 12j) to which a detection signal detected by a detector 4 and a clock signal are input and which output measurement signals corresponding to a time between the detection signal and the clock signal, a control unit 14 that derives and outputs time information related to the detection signal based on the count signal output from the digital counter 20 and the measurement signals output from the TAC circuits 12, and a measurement gate 11 that switches between the TAC circuits 12 to which the detection signal is input, in consideration of dead times of the TAC circuits 12.