知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

38 条记录 (耗时 0.021 秒)

    Mutant purine nucleoside phosphorylase proteins and cellular delivery thereof
    1.
    发明申请
    Mutant purine nucleoside phosphorylase proteins and cellular delivery thereof 有权
    突变型嘌呤核苷磷酸化酶蛋白及其细胞递送

    公开(公告)号：US20050214901A1

    公开(公告)日：2005-09-29

    申请号：US10493729

    申请日：2002-10-28

    申请人： Steven Ealick ,  William Parker ,  John Secrist III ,  Eric Sorscher

    发明人： Steven Ealick ,  William Parker ,  John Secrist III ,  Eric Sorscher

    IPC分类号： A61K47/48 ,  C07H19/16 ,  C07H19/20 ,  C07H21/04 ,  C12N1/21 ,  C12N9/10 ,  C12N9/22 ,  C12N15/74

    CPC分类号： C12N9/1077 ,  A61K47/555 ,  A61K47/67 ,  B82Y5/00 ,  C07H19/16 ,  C07H19/20 ,  C12Y204/02001

    摘要： A host cell stably transformed or transfected by a vector including a DNA sequence encoding for mutant purine nucleoside cleavage enzymes is provided. The transformed or transfected host cell can be used in combination with a purine substrate to treat tumour cells and/or virally infected cells. A nucleotide sequence encoding mutant E. coli derived purine nucleoside phosphorylase proteins which can be used in conjunction with an appropriate substrate to produce toxins which impair abnormal cell growth is also provided. A method is detailed for the delivery of toxin by generation withing target cells or by administration and delivery to the cells from without. Novel purine nucleosides are detailed that yield a cytotoxic purine upn enzymatic cleavage. A synthetic process for nucleosides is also detailed.

    摘要翻译： 提供了通过包含编码突变型嘌呤核苷切割酶的DNA序列的载体稳定转化或转染的宿主细胞。 转化或转染的宿主细胞可以与嘌呤底物组合使用以治疗肿瘤细胞和/或病毒感染的细胞。 还提供了编码突变大肠杆菌衍生的嘌呤核苷磷酸化酶蛋白的核苷酸序列，其可以与适当的底物结合使用以产生损害异常细胞生长的毒素。 详细说明了通过用靶细胞一代递送毒素或通过从没有给予和递送至细胞的方法。 详细介绍了新的嘌呤核苷，其产生细胞毒性嘌呤以进行酶裂解。 还详细介绍了核苷的合成过程。