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    METHOD FOR THE ISOMERISATION OF GLUCOSE
    2.
    发明申请
    METHOD FOR THE ISOMERISATION OF GLUCOSE 审中-公开
    葡萄糖异构体的方法

    公开(公告)号：US20160053289A1

    公开(公告)日：2016-02-25

    申请号：US14779543

    申请日：2014-03-25

    申请人： ANNIKKI GMBH

    发明人： Ortwin Ertl

    IPC分类号： C12P19/02 ,  C07D307/46 ,  C07D307/58

    CPC分类号： C12P19/02 ,  C07D307/46 ,  C07D307/58 ,  C12Y101/01001 ,  C12Y101/01014 ,  C12Y101/01307 ,  C12Y106/01

    摘要： Disclosed is a method for the isomerisation of glucose by reduction to sorbitol and subsequent oxidation to fructose, in which the redox cofactors NAD+/NADH and NADP+/NADPH are regenerated in a one-pot-reaction, wherein one of the two redox cofactors is obtained in the reduced form thereof and the other redox cofactor in the oxidised form thereof as a result of at least two additional enzymatically catalysed redox reactions (product forming reactions) taking place in the same reaction batch, wherein a) in the regeneration reaction, which transfers the reduced cofactor back to its originally oxidised form, oxygen or a compound of the general formula R1C(O)COOH is reduced, and b) in the regeneration reaction, which transfers the oxidised cofactor back to its originally reduced form, a compound of the general formula R2CH(OH)R3 is oxidised, wherein R1, R2 and R3 have different meanings in the compounds, characterised in that a mixture of glucose and fructose is used as a starting material. Furthermore, the use of fructose thus produced in a method for producing furan derivatives is disclosed.

    摘要翻译： 公开了通过还原成山梨糖醇并随后氧化成果糖来异构化葡萄糖的方法，其中氧化还原辅因子NAD + / NADH和NADP + / NADPH在一锅反应中再生，其中得到两种氧化还原辅因子之一 其还原形式和其氧化形式的其它氧化还原辅因子是由于在相同的反应批次中进行至少两次另外的酶促催化的氧化还原反应（产物形成反应）的结果，其中a）在转移的再生反应中 还原的辅助因子被还原成其原始的氧化形式，氧或通式为R 1 C（O）COOH的化合物被还原，b）在将氧化的辅因子转移回原来还原形式的再生反应中， 通式R2CH（OH）R3被氧化，其中R1，R2和R3在化合物中具有不同的含义，其特征在于将葡萄糖和果糖的混合物用作原料 。 此外，公开了在呋喃衍生物的制造方法中使用果糖。

    Enhanced yeast expression using regulatory control sequences from yeast sorbitol dehydrogenase gene
    4.
    发明授权
    Enhanced yeast expression using regulatory control sequences from yeast sorbitol dehydrogenase gene 失效
    使用来自酵母山梨糖醇脱氢酶基因的调控序列增强酵母表达

    公开(公告)号：US6033898A

    公开(公告)日：2000-03-07

    申请号：US998226

    申请日：1992-12-30

    申请人： Aparna V. Sarthy ,  Cynthia W. Schopp

    发明人： Aparna V. Sarthy ,  Cynthia W. Schopp

    IPC分类号： G01N33/531 ,  C07H21/04 ,  C07K14/08 ,  C12N1/19 ,  C12N9/02 ,  C12N9/04 ,  C12N15/09 ,  C12N15/81 ,  C12P21/02 ,  C12Q1/26 ,  C12N15/10 ,  C12N15/31

    CPC分类号： C12N9/0089 ,  C07K14/005 ,  C12N15/81 ,  C12N9/0006 ,  C12Y101/01014 ,  C07K2319/00 ,  C07K2319/61 ,  C12N2795/10022

    摘要： An isolated DNA segment is disclosed which is derived from a Saccharomyces cerevisiae sorbitol dehydrogenase gene and which functions to increase expression of an associated foreign polypeptide when the DNA segment and the gene coding for the foreign polypeptide are operably linked in a vector in such a manner that the vector is replicated and carried by a host yeast cell. The functionally active portion of the segment is under the control (i) a transcriptional regulatory sequence of the sorbitol dehydrogenase gene of Saccharomyces cerevisiae, (ii) a translation initiation regulatory sequence of the sorbitol dehydrogenase gene of Saccharomyces cerevisiae, and (iii) a termination regulatory sequence of the sorbitol dehydrogenase gene of Saccharomyces cerevisiae. Yeast cells containing these regulatory sequences linked to a foreign DNA sequence are grown in medium containing sorbitol under conditions permitting a foreign polypeptide to be expressed.

    摘要翻译： 公开了分离的DNA片段，其衍生自酿酒酵母山梨糖醇脱氢酶基因，并且当DNA片段和编码外来多肽的基因以可操作地连接在载体中时，其作用可增加相关外源多肽的表达， 载体被宿主酵母细胞复制和携带。 （i）酿酒酵母的山梨糖醇脱氢酶基因的转录调节序列，（ii）酿酒酵母的山梨糖醇脱氢酶基因的翻译起始调节序列，和（iii）终止 酿酒酵母山梨糖醇脱氢酶基因的调节序列。 将含有与外来DNA序列连接的调节序列的酵母细胞在允许外源多肽表达的条件下，在含有山梨糖醇的培养基中生长。