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    Enhanced yeast expression using regulatory control sequences from yeast sorbitol dehydrogenase gene
    1.
    发明授权
    Enhanced yeast expression using regulatory control sequences from yeast sorbitol dehydrogenase gene 失效
    使用来自酵母山梨糖醇脱氢酶基因的调控序列增强酵母表达

    公开(公告)号：US6033898A

    公开(公告)日：2000-03-07

    申请号：US998226

    申请日：1992-12-30

    申请人： Aparna V. Sarthy ,  Cynthia W. Schopp

    发明人： Aparna V. Sarthy ,  Cynthia W. Schopp

    IPC分类号： G01N33/531 ,  C07H21/04 ,  C07K14/08 ,  C12N1/19 ,  C12N9/02 ,  C12N9/04 ,  C12N15/09 ,  C12N15/81 ,  C12P21/02 ,  C12Q1/26 ,  C12N15/10 ,  C12N15/31

    CPC分类号： C12N9/0089 ,  C07K14/005 ,  C12N15/81 ,  C12N9/0006 ,  C12Y101/01014 ,  C07K2319/00 ,  C07K2319/61 ,  C12N2795/10022

    摘要： An isolated DNA segment is disclosed which is derived from a Saccharomyces cerevisiae sorbitol dehydrogenase gene and which functions to increase expression of an associated foreign polypeptide when the DNA segment and the gene coding for the foreign polypeptide are operably linked in a vector in such a manner that the vector is replicated and carried by a host yeast cell. The functionally active portion of the segment is under the control (i) a transcriptional regulatory sequence of the sorbitol dehydrogenase gene of Saccharomyces cerevisiae, (ii) a translation initiation regulatory sequence of the sorbitol dehydrogenase gene of Saccharomyces cerevisiae, and (iii) a termination regulatory sequence of the sorbitol dehydrogenase gene of Saccharomyces cerevisiae. Yeast cells containing these regulatory sequences linked to a foreign DNA sequence are grown in medium containing sorbitol under conditions permitting a foreign polypeptide to be expressed.

    摘要翻译： 公开了分离的DNA片段，其衍生自酿酒酵母山梨糖醇脱氢酶基因，并且当DNA片段和编码外来多肽的基因以可操作地连接在载体中时，其作用可增加相关外源多肽的表达， 载体被宿主酵母细胞复制和携带。 （i）酿酒酵母的山梨糖醇脱氢酶基因的转录调节序列，（ii）酿酒酵母的山梨糖醇脱氢酶基因的翻译起始调节序列，和（iii）终止 酿酒酵母山梨糖醇脱氢酶基因的调节序列。 将含有与外来DNA序列连接的调节序列的酵母细胞在允许外源多肽表达的条件下，在含有山梨糖醇的培养基中生长。