公开(公告)号：US5663044A

公开(公告)日：1997-09-02

申请号：US606871

申请日：1996-02-26

Applicant: James Noffsinger ,  Michael J. Pugia ,  Melvin D. Smith

Inventor： James Noffsinger ,  Michael J. Pugia ,  Melvin D. Smith

IPC: G01N33/52 ,  C12Q1/37 ,  C12Q1/44 ,  G01N33/50 ,  C12Q1/00 ,  C12Q1/34 ,  G01N33/53

CPC classification number: C12Q1/37 ,  C12Q1/44 ,  G01N33/5094 ,  C12Q2334/00 ,  C12Q2334/70 ,  G01N2333/966 ,  Y10S435/963 ,  Y10S435/97

Abstract: A composition, method and test device for determining the presence of leukocytes, esterase or protease in a test sample are disclosed. The composition comprises an ester which is hydrolyzed in the presence of leukocyte, esterase, or protease to form a reaction product which couples with a diazonium salt to produce a detectable color change. The composition further comprises the salt of an alkaline earth metal which stabilizes the composition during manufacture and prevents the occurrence of false or background color change due to reactivity of the diazonium salt in the absence of leukocyte, esterase or protease.

Abstract translation: 公开了一种用于确定测试样品中白细胞，酯酶或蛋白酶的存在的组合物，方法和测试装置。 组合物包含在白细胞，酯酶或蛋白酶存在下水解的酯，以形成与重氮盐偶联以产生可检测的颜色变化的反应产物。 组合物还包含碱土金属的盐，其在制造期间稳定组合物，并且防止由于在不存在白细胞，酯酶或蛋白酶的情况下由重氮盐的反应性引起的假或背景颜色变化的发生。

公开(公告)号：US5464739A

公开(公告)日：1995-11-07

申请号：US293723

申请日：1994-08-22

Applicant: Gary M. Johnson ,  Robert J. Schaeper

Inventor： Gary M. Johnson ,  Robert J. Schaeper

IPC: G01N33/50 ,  C07C69/12 ,  C07C69/22 ,  C07C69/67 ,  C07C69/68 ,  C07C69/76 ,  C07C69/92 ,  C07C309/64 ,  C07C309/72 ,  C07D207/36 ,  C07D307/58 ,  C07D333/32 ,  C12Q1/00 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/44 ,  G01N33/52 ,  C07C59/08

CPC classification number: C12Q1/44 ,  C12Q1/37 ,  C12Q2334/00 ,  C12Q2334/70 ,  Y10S435/805 ,  Y10S436/903

Abstract: A composition and method for determining the presence of leukocyte cells, esterase or protease in a test sample are disclosed. The composition contains a lactate ester having the structure ##STR1## wherein A is an alcohol blocking group, and wherein B-O- is a residue of compound B-OH and provides a detectable response when the lactate ester is hydrolyzed; and a buffer. One class of lactate ester has the structure ##STR2## wherein X is O, S or NR.sup.2, R is aryl or lower alkyl, R.sup.1 is hydrogen or lower alkyl, and R.sup.2 is hydrogen, lower alkyl or aryl.

Abstract translation: 公开了一种用于测定测试样品中白细胞，酯酶或蛋白酶的存在的组合物和方法。 该组合物含有具有结构“IMAGE”的乳酸酯，其中A是醇封闭基团，其中B-O-是化合物B-OH的残基，并且当乳酸酯被水解时提供可检测的反应; 和缓冲区。 一类乳酸酯具有结构，其中X是O，S或NR 2，R是芳基或低级烷基，R 1是氢或低级烷基，R 2是氢，低级烷基或芳基。

公开(公告)号：US5352613A

公开(公告)日：1994-10-04

申请号：US132804

申请日：1993-10-07

Applicant: Triantafillos P. Tafas ,  Petros Tsipouras

Inventor： Triantafillos P. Tafas ,  Petros Tsipouras

IPC: G01N33/48 ,  C12Q1/42 ,  G01N21/27 ,  G01N21/59 ,  G01N33/483 ,  G01N33/50

CPC classification number: C12Q1/42 ,  G01N21/5911 ,  C12Q2334/30 ,  C12Q2334/70 ,  Y10T436/25

Abstract: A method and apparatus is provided, whereby the level of an intracellular analyte in a cell containing sample may be determined. The apparatus is comprised of a microscope with an X-Y stage, a video camera, a computer adapted to receive video images from the video camera, and a software program for controlling the apparatus in accordance with the method. The method provided performs digital image processing so as to cause undesired features of a video image obtained by the video camera to be diminished, while desired features are emphasized. A measurement of optical densities of desired features may then be used to determine the level of intracellular analyte.

Abstract translation: 提供了一种方法和装置，由此可以确定含有细胞的样品中的细胞内分析物的水平。 该装置包括具有X-Y平台的显微镜，摄像机，适于从摄像机接收视频图像的计算机以及用于根据该方法控制装置的软件程序。 所提供的方法执行数字图像处理，以便导致由摄像机获得的视频图像的不想要的特征减少，同时强调期望的特征。 然后可以使用所需特征的光密度的测量来确定细胞内分析物的水平。

公开(公告)号：US4772553A

公开(公告)日：1988-09-20

申请号：US875161

申请日：1986-06-17

Applicant: Setsuro Fujii ,  Satoshi Sugiyama ,  Syouzou Sawai

Inventor： Setsuro Fujii ,  Satoshi Sugiyama ,  Syouzou Sawai

IPC: C07C67/00 ,  C07C227/00 ,  C07K5/04 ,  C07K5/06 ,  C07K5/083 ,  C07K5/093 ,  C07K5/103 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/56 ,  C12N9/99 ,  C12Q1/38 ,  C12Q1/44

CPC classification number: C07K5/06026 ,  C07K5/0808 ,  C07K5/0819 ,  C07K5/101 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/56 ,  C12Q2334/30 ,  C12Q2334/70 ,  G01N2333/96444 ,  G01N2333/96455 ,  G01N2333/968 ,  G01N2333/9723 ,  G01N2333/974 ,  G01N2333/976

Abstract: This invention relates to a method for determining an enzyme-, an enzyme inhibitor-, an enzyme activator-, or zymogen-activity, which comprises allowing the enzyme to react with a substrate of the formula ##STR1## (wherein R.sub.1 represents an amino acid of an oligopeptide combined in the form of ester through the C-terminal carboxyl group of said amino acid or oligopeptide and R.sub.2 represents a hydrogen or bromine atom) to hydrolyze the substrate, then allowing the hydrolysis product of the formula ##STR2## to react with a FR-ITR salt [Fast Red-ITR salt (N,N'-diethyl-4-methoxymetanilamide diazonium salt)] to form a pigment, and determining the pigment.

Abstract translation: 本发明涉及一种用于测定酶，酶抑制剂，酶活化剂或酶原活性的方法，其包括使酶与下式的底物反应（其中R 1表示 通过所述氨基酸或寡肽的C末端羧基以酯的形式结合的寡肽的氨基酸，并且R 2表示氢或溴原子）以水解底物，然后使下式的水解产物 与FR-ITR盐[Fast Red-ITR盐（N，N'-二乙基-4-甲氧基甲酰胺重氮盐）]反应，形成颜料，测定颜料。

公开(公告)号：US4758508A

公开(公告)日：1988-07-19

申请号：US710423

申请日：1985-03-11

Applicant: Eugen Schnabel ,  James Travis ,  A. Christopher Skjold

Inventor： Eugen Schnabel ,  James Travis ,  A. Christopher Skjold

IPC: G01N33/68 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/44 ,  C12Q1/38

CPC classification number: C12Q1/37 ,  C12Q1/44 ,  C12Q2334/70 ,  C12Q2337/00 ,  Y10S435/805 ,  Y10S435/81

Abstract: Agent for the detection of esterolytic and/or proteolytic enzymes, containing (a) an amino acid ester or peptide ester of a phenol, as the chromogenic enzyme substrate, and (b) an alcohol as the substance which accelerates the enzymatic cleavage of the amino acid ester bond or peptide ester bond of component (a), characterized in that it contains a salt as the accelerating substance.

Abstract translation: 用于检测含有（a）苯酚的氨基酸酯或肽酯作为显色酶底物的酯水解和/或蛋白水解酶的试剂，和（b）作为加速氨基的酶裂解的物质的醇 （a）的酸酯键或肽酯键，其特征在于其含有作为促进物质的盐。

公开(公告)号：US3684451A

公开(公告)日：1972-08-15

申请号：US3684451D

申请日：1969-08-20

Applicant: MILES LAB

Inventor： LINOLI GIANNI ,  MANNUCCI ENZO SERGIO

IPC: C08F8/30 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q1/52 ,  G01N33/52 ,  C07G7/02 ,  C08F27/08 ,  G01N31/00

CPC classification number: C12Q1/52 ,  C08F8/30 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q2334/30 ,  C12Q2334/70 ,  G01N33/52 ,  Y10S435/805 ,  Y10S435/814 ,  Y10T436/173845 ,  Y10T436/203332

Abstract: A NOVEL INSOLUBLE POLYMERIC DIAZONIUM SALT COLOR DEVELOPER CONSISTING OF A DIAZOTIZED AROMATIC POLYAMINE BOUND BY AN AMIDE LINKAGE TO A CATION EXCHANGE RESIN CAN BE PREPARED BY REACTING A CATION EXCHANGE RESIN CONTAINING ACID GROUPS WITH A HALOGENATING AGENT TO CONVERT SOME OF THE RESIN ACID GROUPS TO ACYL HALIDE GROUPS, REACTING THE RESULTING ACYL HALIDE GROUPS WITH AN AROMATIC POLYAMINE TO FORM A RESIN CONTAINING AMIDE LINKAGES AND FREE AROMATIC AMINO GROUPS, AND THEN DIAZOTIZING THE FREE AROMATIC AMINO GROUPS. THE RESULTING INSOLUBLE COLOR DEVELOPER CAN BE USED TO DETECT COUPLING COMPOUNDS BY FORMING COLORED REACTION PRODUCTS WITH SUCH COUPLING COMPOUNDS. THE COLOR DEVELOPER CAN ALSO BE USED TO DETECT AN ENZYME SYSTEM WHEREIN THE ENZYME CATALYZES A REACTION WITH A SUITABLE SUBSTRATE TO RELEASE A COUPLING COMPOUND WHICH WILL FORM A COLORED REACTION PRODUCT WITH THE INSOLUBLE COLOR DEVELOPER.

公开(公告)号：US09777310B2

公开(公告)日：2017-10-03

申请号：US14980552

申请日：2015-12-28

Applicant: Kumara Venkatanarayana Nibhanipudi

Inventor： Kumara Venkatanarayana Nibhanipudi

IPC: C12Q1/44

CPC classification number: C12Q1/44 ,  C12Q2334/70

Abstract: The present invention relates to the field of infectious diseases. The invention specifically relates to the diagnostic test for acute bacterial pharyngitis. The test is used in screening for Group A Beta Haemolytic Streptococcus by the identifying the presence of leukocyte esterase in the throat. The Leukocyte Esterase Throat Swab Test is compared to the Rapid Step Test for efficiency in terms of fast delivery of results.

公开(公告)号：US20160194682A1

公开(公告)日：2016-07-07

申请号：US14980552

申请日：2015-12-28

Applicant: Kumara Venkatanarayana Nibhanipudi

Inventor： Kumara Venkatanarayana Nibhanipudi

IPC: C12Q1/44

CPC classification number: C12Q1/44 ,  C12Q2334/70

Abstract: The present invention relates to the field of infectious diseases. The invention specifically relates to the diagnostic test for acute bacterial pharyngitis. The test is used in screening for Group A Beta Haemolytic Streptococcus by the identifying the presence of leukocyte esterase in the throat. The Leukocyte Esterase Throat Swab Test is compared to the Rapid Step Test for efficiency in terms of fast delivery of results.

Abstract translation: 本发明涉及传染病领域。 本发明具体涉及急性细菌性咽炎的诊断试验。 该测试用于筛选A组溶血溶血链球菌，通过鉴定喉部中白细胞酯酶的存在。 将白细胞酯酶喉拭子测试与快速步骤测试进行比较，以便快速传递结果。

公开(公告)号：US06203496B1

公开(公告)日：2001-03-20

申请号：US09372876

申请日：1999-08-12

Applicant: Michael R. Gael ,  Sidney Gael ,  William Gael

Inventor： Michael R. Gael ,  Sidney Gael ,  William Gael

IPC: A61B500

CPC classification number: G01N33/528 ,  A61F13/42 ,  A61L15/42 ,  C12Q2334/30 ,  C12Q2334/70 ,  G01N33/64 ,  G01N33/68 ,  G01N33/72 ,  G01N33/84 ,  G01N2333/805

Abstract: Disclosed is a disposable diaper that has one or more chemical reagents provided in the absorbent region of the diaper, which comes into contact with urine when a patient wearing the diaper urinates. These chemical reagents change color when they contact urine containing abnormal levels of substances, such as leukocytes, blood, glucose, nitrites, protein, ketones, bilirubin, or urobilinogen; which substances may indicate the presence of a medical abnormality. Such a color change provides a visible indication of an abnormality in the urine and thus a possible incipient medical condition, such as a urinary tract infection, diabetes mellitus, hematuria, or the like. Such a color change is easily assessed by personnel having limited medical training, allowing such personnel to screen patients for these medical conditions and refer them to the appropriate medical personnel.

Abstract translation: 公开了一种一次性尿布，其具有设置在尿布的吸收区域中的一种或多种化学试剂，当尿布患者排尿时与尿液接触。 当这些化学试剂接触含有异常水平的物质如白细胞，血液，葡萄糖，亚硝酸盐，蛋白质，酮，胆红素或尿胆素原的物质时，它们会变色。 哪些物质可能表明存在医疗异常。 这种颜色变化提供了尿液异常的可视指示，因此提供了可能的初期医学状况，例如尿路感染，糖尿病，血尿等。 这种颜色变化可以由具有有限医疗培训的人员轻松评估，允许这些人员筛选患者的这些医疗状况，并将其转交给适当的医务人员。

公开(公告)号：US5981206A

公开(公告)日：1999-11-09

申请号：US886263

申请日：1992-05-20

Applicant: Thomas Charles Arter ,  Mohan S Saini

Inventor： Thomas Charles Arter ,  Mohan S Saini

IPC: C12Q1/42 ,  G01N21/77 ,  G01N31/22 ,  C12Q1/00

CPC classification number: C12Q1/42 ,  C12Q2334/30 ,  C12Q2334/70

Abstract: A dry analytical element has been prepared for the assay of prostatic acid phosphatase at a pH of from about 3 to about 6.5. The element can be a single porous spreading zone or a multilayer structure. Within the element is a relatively nonhygroscopic aromatic phosphate substrate for the analyte which produces a phenol reaction product. This product is reacted with a diazonium or tetrazolium salt, also in the element, to produce a chromophore for detection. A buffer in the element maintains it at the proper pH during the assay.

Abstract translation: 已经制备了干燥的分析元件用于在约3至约6.5的pH下测定前列腺酸性磷酸酶。 元件可以是单个多孔铺展区或多层结构。 元素内是用于分析物的相对不吸湿的芳族磷酸盐底物，其产生苯酚反应产物。 该产物也与元素中的重氮鎓或四唑鎓盐反应，以产生发色团进行检测。 元素中的缓冲液在测定期间将其保持在适当的pH。