公开(公告)号：US3859341A

公开(公告)日：1975-01-07

申请号：US11853171

申请日：1971-02-24

申请人： KABI AB

发明人： JONSSON NILS AKE ,  MERENYI FERENC ,  WESTLUND LARS-ERIK

IPC分类号： C07C217/84 ,  C07D223/16 ,  C12Q1/28 ,  C12Q1/54 ,  G01N31/22 ,  C07C101/44

CPC分类号： C07D223/16 ,  C12Q1/28 ,  C12Q1/54 ,  C12Q2326/10

摘要： Disclosed are disubstituted benzidine derivatives having on both the 3- and 3''-position a substituent W-X-A-On-, subscript n is zero or one, A is a divalent lower alkylene chain with from about 2 to about 7 carbons, X is the divalent carbonyloxy or sulfonyloxy group, W is hydrogen or other chromogen-reactiveindicator compatible cation; and the chromogen-reactive-indicator compatible acid addition salts of those benzidine derivatives. Also disclosed is the improvement in an analytical test, wherein is used a color change reagent preparation comprising peroxidase, an oxygen-oxidoreductase specific to a particular substance to be tested and for which there exists a specific oxygenoxidoreductase, and a chromogen-reactive-indicator which is one of the foregoing disubstituted benzidine derivatives or a chromogen-reactive-indicator compatible acid addition salt of it.

公开(公告)号：US3971702A

公开(公告)日：1976-07-27

申请号：US536867

申请日：1974-12-27

申请人： Hideyuki Maekawa ,  Kaoru Ishitobi

发明人： Hideyuki Maekawa ,  Kaoru Ishitobi

IPC分类号： C12Q1/28 ,  C12Q1/54 ,  G01N33/52 ,  G01N31/14 ,  C12K1/04 ,  C12K1/10

CPC分类号： C12Q1/28 ,  C12Q1/54 ,  G01N33/52 ,  C12Q2326/00 ,  C12Q2326/10 ,  C12Q2326/14 ,  Y10S435/805

摘要： A diagnostic composition is prepared which contains as a chromogen a diamino-biphenyl derivative in which at least one of the hydrogen atoms in the amino groups thereof is replaced by a lower alkyl group by reaction with an alkylating agent. A diagnosing instrument is prepared by impregnating a bibulous, porous support in a solution comprising the diagnostic composition and optionally a saccharide oxidase, peroxidase and a guaiac, and, if necessary, a buffer, a surfactant, a background adjusting dye and/or an agent for increasing the viscosity of the composition. This diagnosing instrument can be handled in a simple and convenient way and is used to identify semi-quantitatively a saccharide in body fluids or excrements of humans or animals with reliability.

摘要翻译： 制备诊断组合物，其包含作为色原的二氨基 - 联苯衍生物，其中氨基中的至少一个氢原子被烷基化剂与低级烷基取代。 通过在包含诊断组合物和任选的糖氧化酶，过氧化物酶和愈创木酚的溶液中浸渍吸水多孔载体，并且如果需要，将缓冲液，表面活性剂，背景调节染料和/或试剂 以增加组合物的粘度。 该诊断仪器可以以简单方便的方式处理，并可用于半可定量地识别体液或人或动物粪便中的糖类。

公开(公告)号：US5418138A

公开(公告)日：1995-05-23

申请号：US212415

申请日：1994-03-11

申请人： Phillip C. Miller ,  Michael J. Degroff ,  Michael J. Gizinski ,  James A. Rybski ,  Pamela S. Vandivort ,  Anthony L. Hartman

发明人： Phillip C. Miller ,  Michael J. Degroff ,  Michael J. Gizinski ,  James A. Rybski ,  Pamela S. Vandivort ,  Anthony L. Hartman

IPC分类号： C12N9/96 ,  C12Q1/28 ,  G01N1/30 ,  G01N33/48 ,  G01N33/53 ,  G01N33/531 ,  G01N33/535 ,  G01N33/543 ,  G01N33/567 ,  G01N33/58 ,  G01N35/00

CPC分类号： G01N33/535 ,  C12N9/96 ,  C12Q1/28 ,  G01N1/30 ,  G01N33/54393 ,  G01N33/567 ,  G01N33/581 ,  C12Q2326/10 ,  G01N2035/00079 ,  G01N2035/00277 ,  Y10S435/96 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S436/825 ,  Y10T436/112499 ,  Y10T436/25 ,  Y10T436/25125

摘要： The present invention provides an improved method for staining slides using immunochemical reagents. The method comprises the following steps. The assay region of a slide (the region containing the tissue section)is washed with an improved rinsing solution comprising water and a detergent. An evaporation inhibitor liquid is applied to the slide to cover the assay region. For antigens requiring unmasking, the tissue section is combined with an improved, stabilized proteolytic enzyme solution. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. A primary antibody in an improved diluent containing globulins from the same species as a second antibody is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied. A labeled second antibody in the improved diluent is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. Color development reagents, including a stabilized peroxidase chromophore formulation, are combined with the tissue section for a time sufficient for color development. The stabilized peroxidase chromophore formulation consists essentially of a peroxidase chromophore, at a concentration in the working range of the enzyme, an acidic buffer, a reducing agent, and a glycol. Preferred chromophores include 3,3'-diaminobenzidine and tetrahydrochloride (DAB) and 3-amino-9-ethylcarbazole (AEC). After color development, the tissue section is washed and is then ready for analysis.

摘要翻译： 本发明提供了使用免疫化学试剂对载玻片进行染色的改进方法。 该方法包括以下步骤。 载玻片的测定区域（含有组织切片的区域）用包含水和洗涤剂的改进的冲洗溶液洗涤。 将蒸发抑制剂液体施加到载玻片上以覆盖测定区域。 对于需要揭发的抗原，将组织切片与改良的稳定的蛋白水解酶溶液组合。 将载玻片漂洗，并将蒸发抑制剂液体重新施加到载玻片上。 将来自与第二抗体相同物种的球蛋白的改进的稀释剂中的一抗与组织切片组合足够的时间，足以基本上完​​全抗体结合。 将载玻片漂洗，并重新施加蒸发抑制剂液体。 将改进的稀释剂中的标记的第二抗体与组织切片组合足够长的时间以使基本上完全的抗体结合。 将载玻片漂洗，并将蒸发抑制剂液体重新施加到载玻片上。 包括稳定的过氧化物酶发色团配方的显色试剂与组织切片组合足够的时间用于显色。 稳定的过氧化物酶发色团配方主要由过氧化物酶发色团组成，浓度在酶的工作范围内，酸性缓冲液，还原剂和二醇。 优选的发色团包括3,3'-二氨基联苯胺和四盐酸盐（DAB）和3-氨基-9-乙基咔唑（AEC）。 在显色后，洗涤组织切片，然后准备分析。

公开(公告)号：US5322771A

公开(公告)日：1994-06-21

申请号：US924053

申请日：1992-08-31

申请人： James A. Rybski ,  Pamela S. Vandivort ,  Anthony L. Hartman ,  Phillip C. Miller ,  Michael J. Degroff ,  Michael J. Gizinski

发明人： James A. Rybski ,  Pamela S. Vandivort ,  Anthony L. Hartman ,  Phillip C. Miller ,  Michael J. Degroff ,  Michael J. Gizinski

IPC分类号： C12N9/96 ,  C12Q1/28 ,  G01N1/30 ,  G01N33/48 ,  G01N33/53 ,  G01N33/531 ,  G01N33/535 ,  G01N33/543 ,  G01N33/567 ,  G01N33/58 ,  G01N35/00 ,  C12Q1/37 ,  G01N1/34

CPC分类号： G01N33/535 ,  C12N9/96 ,  C12Q1/28 ,  G01N1/30 ,  G01N33/54393 ,  G01N33/567 ,  G01N33/581 ,  C12Q2326/10 ,  G01N2035/00079 ,  G01N2035/00277 ,  Y10S435/96 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S436/825 ,  Y10T436/112499 ,  Y10T436/25 ,  Y10T436/25125

摘要： The present invention provides an improved method for staining slides using immunochemical reagents. The method comprises the following steps. The assay region of a slide (the region containing the tissue section) is washed with an improved rinsing solution comprising water and a detergent. An evaporation inhibitor liquid is applied to the slide to cover the assay region. For antigens requiring unmasking, the tissue section is combined with an improved, stabilized proteolytic enzyme solution. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. A primary antibody in an improved diluent containing globulins from the same species as a second antibody is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied. A labeled second antibody in the improved diluent is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. Color development reagents, including, in a preferred embodiment, a stabilized diaminobenzidine (DAB) solution, are combined with the tissue section for a time sufficient for color development. Following rinsing, the tissue section is ready for analysis.

摘要翻译： PCT No.PCT / US91 / 01108 Sec。 371日期：1992年8月31日 102（e）日期1992年8月31日PCT 1991年2月27日PCT PCT。 出版物WO91 / 13336 1991年9月5日。本发明提供了一种使用免疫化学试剂染色载玻片的改进方法。 该方法包括以下步骤。 载玻片的测定区域（含有组织切片的区域）用包含水和洗涤剂的改进的冲洗溶液洗涤。 将蒸发抑制剂液体施加到载玻片上以覆盖测定区域。 对于需要揭发的抗原，将组织切片与改良的稳定的蛋白水解酶溶液组合。 将载玻片漂洗，并将蒸发抑制剂液体重新施加到载玻片上。 将来自与第二抗体相同物种的球蛋白的改进的稀释剂中的一抗与组织切片组合足够的时间，足以基本上完​​全抗体结合。 将载玻片漂洗，并重新施加蒸发抑制剂液体。 将改进的稀释剂中的标记的第二抗体与组织切片组合足够长的时间以使基本上完全的抗体结合。 将载玻片漂洗，并将蒸发抑制剂液体重新施加到载玻片上。 在优选的实施方案中，将显色剂（包括稳定的二氨基联苯胺（DAB）溶液）与组织切片组合足够的时间用于显色。 冲洗后，组织部分准备好进行分析。

公开(公告)号：US4385114A

公开(公告)日：1983-05-24

申请号：US236487

申请日：1981-02-23

申请人： Werner Guthlein ,  Walter Rittersdorf ,  Hugo Tiedemann ,  Peter Vogel ,  Wolfgang Werner

发明人： Werner Guthlein ,  Walter Rittersdorf ,  Hugo Tiedemann ,  Peter Vogel ,  Wolfgang Werner

IPC分类号： C07C211/50 ,  C07C67/00 ,  C07C209/00 ,  C07C209/54 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  G01N21/78 ,  G01N31/00 ,  G01N31/22 ,  G01N33/50 ,  C12Q1/60 ,  C12Q1/62

CPC分类号： C12Q1/26 ,  C12Q1/28 ,  G01N31/228 ,  C12Q2326/10 ,  C12Q2326/12 ,  Y10S435/805 ,  Y10S436/81 ,  Y10T436/144444 ,  Y10T436/206664

摘要： The detection and determination of (a) hydrogen peroxide or of a substance which reacts with the formation of hydrogen peroxide or (b) of peroxidase or a peroxidatively active substance, is carried out by contacting an unknown sample with a chromogen and, in case (a), with a peroxidatively active substance or in case (b) with hydrogen peroxide or a substance which reacts with the formation of hydrogen peroxide, and evaluating the coloration produced by the reaction, wherein, as the chromogen, there is used at least one 3,3',5,5'-tetraalkylbenzidine compound of the formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are straight-chained or branched alkyl radicals containing up to 6 and preferably up to 4 carbon atoms.

摘要翻译： （a）过氧化氢或与过氧化氢或过氧化物酶或过氧化物活性物质的（b）反应形成的物质的检测和测定是通过将​​未知样品与色原体接触进行的，如果（ a），具有过氧化活性物质或在（b）过氧化氢或与形成过氧化氢反应的物质的情况下，以及评价由反应产生的着色，其中作为色原，使用至少一种 具有下式的3,3'，5,5'-四烷基联苯胺化合物：其中R 1，R 2，R 3和R 4可以相同或不同，是含有至多6个的直链或支链烷基，优选 最多4个碳原子。

公开(公告)号：US09588123B2

公开(公告)日：2017-03-07

申请号：US14683693

申请日：2015-04-10

申请人： SurModics IVD, Inc.

发明人： Sean Lundquist

IPC分类号： G01N33/58 ,  C12Q1/26 ,  C12Q1/28

CPC分类号： G01N33/583 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q2326/10 ,  G01N33/581

摘要： The disclosure is directed to compositions, kits, and methods for performing colorimetric analysis. A composition for a colorimetric assay can be prepared that includes an aromatic amine chromogenic substrate and a zwitterionic solubilization reagent that is N,N,N-trimethylglycine (betaine). The zwitterionic solubilization reagent is highly useful as a non-encapsulating solubilization reagent and provided faster kinetics and increased dye content of an aromatic amine chromogenic substrate in an enzymatic reaction. In turn, this can result in improvements in detection of an analyte in a sample and also can increase the sample throughput. The aromatic amine chromogenic substrate and N,N,N-trimethylglycine solubilization reagent can be used in assays such as ELISAs in order to provide a more accurate and faster detection of analytes in a biological sample.

摘要翻译： 本公开涉及用于进行比色分析的组合物，试剂盒和方法。 可以制备用于比色测定的组合物，其包括芳族胺显色底物和N，N，N-三甲基甘氨酸（甜菜碱）的两性离子溶解试剂。 两性离子溶解试剂作为非包封的增溶剂是非常有用的，并提供酶反应中芳族胺显色底物的更快的动力学和增加的染料含量。 反过来，这可以导致样品中分析物的检测的改进，并且还可以增加样品通量。 芳族胺显色底物和N，N，N-三甲基甘氨酸增溶剂可用于诸如ELISA的测定中，以便提供对生物样品中分析物的更准确和更快速的检测。

公开(公告)号：US4132527A

公开(公告)日：1979-01-02

申请号：US708631

申请日：1976-07-26

申请人： Hideyuki Maekawa ,  Kaoru Ishitobi

发明人： Hideyuki Maekawa ,  Kaoru Ishitobi

IPC分类号： C12Q1/28 ,  C12Q1/54 ,  G01N33/52 ,  G01N33/72 ,  C07C87/28 ,  C07C87/64 ,  G01N31/22 ,  G01N33/16

CPC分类号： C12Q1/54 ,  C12Q1/28 ,  G01N33/52 ,  G01N33/725 ,  C12Q2326/00 ,  C12Q2326/10 ,  C12Q2326/14 ,  Y10S436/904

摘要： A diagnostic composition containing as a chromogen a diamino-biphenyl derivative in which at least one of the hydrogen atoms in the amino groups thereof is replaced by a lower alkyl group by reaction with an alkylating agent. The diagnosing instrument is prepared by impregnating a bibulous, porous support in a solution comprising the diagnostic composition and optionally a saccharide oxidase, peroxidase and a guaiac, and, if necessary, a buffer, a surfactant, a background adjusting dye and/or an agent for increasing the viscosity of the composition. This diagnosing instrument can be handled in a simple and convenient way and is used to identify semi-quantitatively a saccharide in body fluids or excrements of humans or animals with reliability.

摘要翻译： 一种诊断组合物，其含有二氨基 - 联苯衍生物，其中氨基中的至少一个氢原子被烷基化剂与低级烷基取代。 通过在包含诊断组合物和任选的糖氧化酶，过氧化物酶和愈创木酚的溶液中浸渍吸水多孔载体并且如果需要的话，将缓冲液，表面活性剂，背景调节染料和/或剂 以增加组合物的粘度。 该诊断仪器可以以简单方便的方式处理，并可用于半可定量地识别体液或人或动物粪便中的糖类。

公开(公告)号：US4066408A

公开(公告)日：1978-01-03

申请号：US766961

申请日：1977-02-09

申请人： Nils Ake Jonsson ,  Ferenc Merenyi ,  Lars-Erik Westlund

发明人： Nils Ake Jonsson ,  Ferenc Merenyi ,  Lars-Erik Westlund

IPC分类号： C12Q1/28 ,  C12Q1/54 ,  C12Q1/62 ,  G01N21/06 ,  G01N31/22

CPC分类号： C12Q1/28 ,  C12Q1/54 ,  C12Q1/62 ,  C12Q2326/10

摘要： An analytical test, color change reagent preparation comprising peroxidase, an oxygen-oxidoreductase specific to a particular substance to be tested and a chromogen-reactive-indicator formula selected from (a) those having the general formula ##STR1## wherein X is the divalent carbonyloxy or sulfonyloxy group, W is hydrogen or another cation compatible with the aforesaid other ingredients of said reagent preparation, the subscript n is 0 or 1, and (i) when X is the carbonyloxy group, A is a divalent alkylene chain with from 2 to about 7 carbons, and (ii) when X is the sulfonyloxy group, n is 0 when A is the divalent alkylene chain of from 2 to about 7 carbons, and n is 1 when A is straight chain trimethylene or tetramethylene, and (b) the acid addition salts of said disubstituted benzidine derivative, compatible with the aforesaid ingredients of the chromogen-reactive-indicator, when X is the carbonyloxy group.The reagent preparations may be used in assaying organic substances such as sugars, uric acid, an amino acid and pyridoxaminophosphate.

公开(公告)号：US4008267A

公开(公告)日：1977-02-15

申请号：US515195

申请日：1974-10-16

申请人： Nils Ake Jonsson ,  Ferenc Merenyl ,  Lars-Erik Westlund

发明人： Nils Ake Jonsson ,  Ferenc Merenyl ,  Lars-Erik Westlund

IPC分类号： C07D223/16 ,  C12Q1/26 ,  C12Q1/28 ,  C07C143/64 ,  C07C143/56

CPC分类号： C07D223/16 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q2326/10

摘要： Disclosed are benzidine derivatives substituted in each of the 3,3'-positions by the substituent ##STR1## wherein W is hydrogen or an alkali metal, the subscript n is zero when A is the divalent branched chain alkylene having from 2 to about 7 carbon atoms, and n is one when A is straight chain divalent alkylene with 3 or 4 carbons. Also disclosed are the corresponding derivatives wherein the sulfonyl group is replaced by the carbonyl group. Further disclosed are chromogen-reactive indicator compositions having as their chromogen constituent one of the foregoing benzidine derivatives. These compositions (1) are useful to test for a substance for which there exists a specific oxygen-oxidoreductase, such as a sugar, an amino acid, uric acid, and the like, and thus (2) contain also a peroxidase, a hydrogen-peroxide-oxidoreductase, and a specific oxygen-oxidoreductase for the specific material in the testing of which the composition is to be used.

摘要翻译： 公开了通过取代基“IMAGE”取代的3,3'-位各自的联苯胺衍生物，其中W是氢或碱金属，当A是具有2至约7个碳原子的二价支链亚烷基时，下标n为0 原子，当A是具有3或4个碳原子的直链二价亚烷基时，n是1。 还公开了相应的衍生物，其中磺酰基被羰基取代。 进一步公开的是具有上述联苯胺衍生物之一作为其色原成分的色素反应性指示剂组合物。 这些组合物（1）可用于测试存在特定氧 - 氧化还原酶（例如糖，氨基酸，尿酸等）的物质，因此（2）还含有过氧化物酶，氢 过氧化物 - 氧化还原酶，以及用于要使用该组合物的试验中的具体材料的特定氧 - 氧化还原酶。

公开(公告)号：US20150293110A1

公开(公告)日：2015-10-15

申请号：US14683693

申请日：2015-04-10

申请人： SurModics IVD, Inc.

发明人： Sean Lundquist

IPC分类号： G01N33/58

CPC分类号： G01N33/583 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q2326/10 ,  G01N33/581

摘要： The disclosure is directed to compositions, kits, and methods for performing colorimetric analysis. A composition for a colorimetric assay can be prepared that includes an aromatic amine chromogenic substrate and a zwitterionic solubilization reagent that is N,N,N-trimethylglycine (betaine). The zwitterionic solubilization reagent is highly useful as a non-encapsulating solubilization reagent and provided faster kinetics and increased dye content of an aromatic amine chromogenic substrate in an enzymatic reaction. In turn, this can result in improvements in detection of an analyte in a sample and also can increase the sample throughput. The aromatic amine chromogenic substrate and N,N,N-trimethylglycine solubilization reagent can be used in assays such as ELISAs in order to provide a more accurate and faster detection of analytes in a biological sample.

摘要翻译： 本公开涉及用于进行比色分析的组合物，试剂盒和方法。 可以制备用于比色测定的组合物，其包括芳族胺显色底物和N，N，N-三甲基甘氨酸（甜菜碱）的两性离子溶解试剂。 两性离子溶解试剂作为非包封的增溶剂是非常有用的，并提供酶反应中芳族胺显色底物的更快的动力学和增加的染料含量。 反过来，这可以导致样品中分析物的检测的改进，并且还可以增加样品通量。 芳族胺显色底物和N，N，N-三甲基甘氨酸增溶剂可用于诸如ELISA的测定中，以便提供对生物样品中分析物的更准确和更快速的检测。