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    Prostate cancer cell lines
    2.
    发明授权
    Prostate cancer cell lines 失效
    前列腺癌细胞系

    公开(公告)号：US06777230B2

    公开(公告)日：2004-08-17

    申请号：US09919196

    申请日：2001-07-31

    申请人： Ming-Fong Lin

    发明人： Ming-Fong Lin

    IPC分类号： C12N502

    CPC分类号： C12N5/0683 ,  C12N5/0693 ,  C12N2502/246 ,  C12N2503/00 ,  G01N33/57434

    摘要： Novel human prostate cancer-associated neuroendocrine (NE)-like cell lines are provided that were derived via a process that resembles clinical androgen ablation therapy for advanced prostate cancer.

    摘要翻译： 提供了新的人前列腺癌相关神经内分泌（NE）样细胞系，其通过类似于晚期前列腺癌的临床雄激素消除治疗的过程得到。

    Production and use of dopaminergic cells to treat dopaminergic deficiencies
    3.
    发明申请
    Production and use of dopaminergic cells to treat dopaminergic deficiencies 审中-公开
    生产和使用多巴胺能细胞治疗多巴胺能缺乏症

    公开(公告)号：US20020192194A1

    公开(公告)日：2002-12-19

    申请号：US10000452

    申请日：2001-12-04

    发明人： Michael McGrogan ,  Gary Snable

    IPC分类号： A61K048/00 ,  C12N005/08

    CPC分类号： C12N5/0619 ,  A61K35/12 ,  C12N2500/12 ,  C12N2501/385 ,  C12N2502/246

    摘要： Differentiated neuronal cells suitable for transplantation in individuals with a dopamine deficiency are derived from progenitor cells. The progenitor cells are treated with at least one inducing agent such as retinoic acid for a time period sufficient to optimize expression of tyrosine hydroxylase. The cells intended for transplantation are optionally treated with a lithium salt to enhance bcl-2 production and survival. Optionally, the progenitor cells are co-cultured with Sertoli cells, bone marrow stem cells, or a combination thereof. The transplantation-ready cells are isolated and harvested. The resulting neuronal cells are purified and have a phenotype optimized to treat a dopaminergic deficiency, such as Parkinson's Disease. Optionally the neuronal cells can be implanted with Sertoli cells, bone marrow stem cells or a combination thereof. A purified human dopaminergic cell type is obtained by culturing NT2 cells and treated for about three weeks with an inducing agent, culturing for about two weeks with growth media without an inducing agent or mitotic inhibitor, culturing for about one week with at least one mitotic inhibitor, harvesting and placing in a diluent.

    摘要翻译： 适合于多巴胺缺乏症患者移植的分化神经元细胞来源于祖细胞。 用至少一种诱导剂如视黄酸处理祖细胞足够的时间以优化酪氨酸羟化酶的表达。 用于移植的细胞任选用锂盐处理以增强bcl-2的产生和存活。 任选地，祖细胞与支持细胞，骨髓干细胞或其组合共培养。 分离并收获移植准备的细胞。 所得神经元细胞被纯化并具有优化的表型以治疗多巴胺能缺乏症，例如帕金森病。 任选地，神经元细胞可以植入支持细胞，骨髓干细胞或其组合。 通过培养NT2细胞并用诱导剂处理大约三周，获得纯化的人多巴胺能细胞类型，用不含诱导剂或有丝分裂抑制剂的生长培养基培养约两周，用至少一种有丝分裂抑制剂培养约一周 ，收获并放入稀释剂中。

    Methods of treating disease using sertoli cells and allografts or xenografts
    5.
    发明授权
    Methods of treating disease using sertoli cells and allografts or xenografts 失效
    使用sertoli细胞和同种异体移植物或异种移植物治疗疾病的方法

    公开(公告)号：US5843430A

    公开(公告)日：1998-12-01

    申请号：US467341

    申请日：1995-06-06

    申请人： Helena P. Selawry

    发明人： Helena P. Selawry

    IPC分类号： A61K38/28 ,  A61K35/12 ,  A61K35/39 ,  A61K35/407 ,  A61K35/48 ,  A61K38/00 ,  A61K38/13 ,  A61K48/00 ,  A61P3/08 ,  A61P3/10 ,  C12N5/071 ,  A01N63/00 ,  G01N33/53

    CPC分类号： C12N5/0683 ,  A61K35/39 ,  A61K35/48 ,  A61K38/13 ,  A61K38/28 ,  C12N5/0676 ,  C12N5/0677 ,  A61K48/00 ,  C12N2502/246 ,  C12N2510/00 ,  C12N2510/04 ,  Y10S435/975

    摘要： The present invention describes a method of treating a disease that results from a deficiency of a biological factor which comprises administering to a mammal Sertoli cells and cells that produce the biological factor. In particular, the present invention describes a method of treating diabetes mellitus by transplanting pancreatic islet of Langerhans cells in conjunction with Sertoli cells to create an immunologically privileged site. A method of creating an immunologically privileged site in a mammal for cellular transplants is further described by the present invention. A pharmaceutical composition and compartmentalized kit comprising Sertoli cells and cells that produce a biological factor is also provided.

    摘要翻译： 本发明描述了一种治疗由生物因子缺乏引起的疾病的方法，其包括对产生生物因子的哺乳动物Sertoli细胞和细胞施用。 特别地，本发明描述了通过移植胰腺细胞胰岛胰岛细胞与Sertoli细胞结合来产生免疫特异性位点来治疗糖尿病的方法。 通过本发明进一步描述了在哺乳动物中为细胞移植创建免疫特异性位点的方法。 还提供了包含产生生物因子的Sertoli细胞和细胞的药物组合物和分隔试剂盒。