公开(公告)号：US20150191710A1

公开(公告)日：2015-07-09

申请号：US14409633

申请日：2013-07-02

Applicant: IPROGEN BIOTECH INC.

Inventor： Keun Ho Lee ,  Leo Yen-Cheng Lin ,  Aikun Wang

IPC: C12N9/16 ,  C07K16/18 ,  C07K14/435 ,  C07K16/10

CPC classification number: C12N9/16 ,  C07K14/43504 ,  C07K16/1072 ,  C07K16/18 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/10 ,  C07K2319/30 ,  C07K2319/60 ,  C12N15/85 ,  C12Y301/03001

Abstract: A secretion signal peptide sequence (SP) in combination with a cleavage inhibition sequence (CIS) fused to a structural gene sequence in a recombinant expression system can be used to express a full length protein with an SP in a cell. Such a fusion protein may be purified to homogeneity from a membrane fraction of the cell. The SP in combination with the CIS is a protein transduction domain that exhibits superior intracellular protein transduction efficiency when the SP precedes the CIS in a N to C-terminus direction.

Abstract translation: 与重组表达系统中的结构基因序列融合的切割抑制序列（CIS）的分泌信号肽序列（SP）可以用于在细胞中用SP表达全长蛋白。 这样的融合蛋白可以从细胞的膜部分纯化成均质。 与CIS组合的SP是蛋白转导结构域，当SP在N至C末端方向上先于CIS时，其表现出优异的细胞内蛋白转导效率。

公开(公告)号：US20130164250A1

公开(公告)日：2013-06-27

申请号：US13739421

申请日：2013-01-11

Applicant: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (C.N.R.S.)

Inventor： YASEMIN ATAMAN-ONAL ,  THIERRY DELAIR ,  GENEVIEVE INCHAUSPE ,  PASCALE JEANNIN ,  GLAUCIA PARANHOS-BACCALA ,  BERNARD VERRIER

IPC: A61K47/48

CPC classification number: C07K16/40 ,  A61K9/167 ,  A61K9/5153 ,  A61K9/5192 ,  A61K39/00 ,  A61K47/34 ,  A61K47/6935 ,  A61K2039/6093 ,  C07K14/005 ,  C07K16/1054 ,  C07K16/1072 ,  C12N2740/16222 ,  C12N2740/16322 ,  C12N2770/24222 ,  G01N33/54346

Abstract: Nonlamellar bioresorbable microparticles to which protein substances are bonded, and a method for preparing the microparticles, comprising: (i) preparing the microparticles from at least one bioresorbable polymer without stabilizer and without surfactant; and (ii) bonding the protein substances to the microparticles obtained in step (i) without surfactant.

Abstract translation: 蛋白质物质结合的非平面生物可再吸收微粒及其制备方法，其特征在于，包括：（i）从不含稳定剂和无表面活性剂的至少一种生物可再吸收聚合物制备微粒; 和（ii）将蛋白质物质结合到没有表面活性剂的步骤（i）中获得的微粒子。

公开(公告)号：US20080124331A1

公开(公告)日：2008-05-29

申请号：US11733861

申请日：2007-04-11

Applicant: Belinda Cairns ,  Ruihuan Chen ,  Gretchen Frantz ,  Kenneth Hillan ,  Hartmut Koeppen ,  Heidi Phillips ,  Paul Polakis ,  Susan Spencer ,  Victoria Smith ,  P. Williams ,  Thomas Wu ,  Zemin Zhang ,  Mark Sliwkowski

Inventor： Belinda Cairns ,  Ruihuan Chen ,  Gretchen Frantz ,  Kenneth Hillan ,  Hartmut Koeppen ,  Heidi Phillips ,  Paul Polakis ,  Susan Spencer ,  Victoria Smith ,  P. Williams ,  Thomas Wu ,  Zemin Zhang ,  Mark Sliwkowski

IPC: A61K31/70 ,  A61K39/395 ,  A61P43/00 ,  C07H21/04 ,  C07K16/18 ,  C12N5/06 ,  G01N33/574

CPC classification number: G01N33/57484 ,  A61K47/60 ,  A61K47/6803 ,  A61K47/6809 ,  A61K47/6817 ,  A61K47/6839 ,  A61K47/6851 ,  A61K47/6859 ,  A61K51/1006 ,  A61K51/1018 ,  A61K2039/505 ,  C07K16/1072 ,  C07K16/18 ,  C07K16/30 ,  C07K16/303 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/77

Abstract: The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same.

公开(公告)号：US20070059681A1

公开(公告)日：2007-03-15

申请号：US10570948

申请日：2004-09-21

Applicant: Yasemin Ataman-Onal ,  Thierry Delair ,  Genevieve Inchauspe ,  Pascale Jeannin ,  Glaucia Paranhos-Baccala ,  Bernard Verrier

Inventor： Yasemin Ataman-Onal ,  Thierry Delair ,  Genevieve Inchauspe ,  Pascale Jeannin ,  Glaucia Paranhos-Baccala ,  Bernard Verrier

IPC: A61K39/21 ,  C12Q1/70 ,  C07K16/10

CPC classification number: C07K16/40 ,  A61K9/167 ,  A61K9/5153 ,  A61K9/5192 ,  A61K39/00 ,  A61K47/34 ,  A61K47/6935 ,  A61K2039/6093 ,  C07K14/005 ,  C07K16/1054 ,  C07K16/1072 ,  C12N2740/16222 ,  C12N2740/16322 ,  C12N2770/24222 ,  G01N33/54346

Abstract: The present invention relates to a method for preparing nonlamellar bioresorbable microparticles to which protein substances are bonded, characterized in that it comprises the steps of: (i) preparing said microparticles from at least one bioresorbable polymer without stabilizer and without surfactant, and (ii) bonding said protein substances to the microparticles obtained in step (i) without surfactant. It further relates to the bioresorbable microparticles thus obtained and use thereof in diagnosis and therapy.

Abstract translation: 本发明涉及一种制备与蛋白质物质结合的非水溶性生物可再吸收微粒的方法，其特征在于其包括以下步骤：（i）从不含稳定剂和不含表面活性剂的至少一种生物可再吸收聚合物制备所述微粒，和（ii） 将所述蛋白质物质与不含表面活性剂的步骤（i）中获得的微粒结合。 它还涉及由此获得的生物可再吸收的微粒及其在诊断和治疗中的用途。

公开(公告)号：US20060240028A1

公开(公告)日：2006-10-26

申请号：US10511990

申请日：2003-04-21

Applicant: Abraham Loyter ,  Adolf Graessman ,  Ahuva Nissim ,  Alexander Krichevsky ,  Nechama Zakai

Inventor： Abraham Loyter ,  Adolf Graessman ,  Ahuva Nissim ,  Alexander Krichevsky ,  Nechama Zakai

IPC: A61K39/42 ,  A61K39/21 ,  C07K14/16 ,  C07K16/10

CPC classification number: C07K16/1072 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2318/10

Abstract: The present invention relates to a substance that specifically binds a nuclear localization signal (NLS)-containing molecule. Said substance may be a naturally occurring, synthetic or recombinant antibody, or it may be a protein, a peptide or a small molecule. Preferably, said NLS-containing molecule is a HIV-1 protein, more preferably Vpr or Tat. In addition, the present invention relates to a composition or a vaccine comprising the substance of the invention, as well as methods of inhibiting viral infection through the administration thereof.

Abstract translation: 本发明涉及特异性结合含核定位信号（NLS）的分子的物质。 所述物质可以是天然存在的，合成的或重组的抗体，或者它可以是蛋白质，肽或小分子。 优选地，所述含有NLS的分子是HIV-1蛋白质，更优选为Vpr或Tat。 此外，本发明涉及包含本发明物质的组合物或疫苗，以及通过其施用来抑制病毒感染的方法。

公开(公告)号：US20060211108A1

公开(公告)日：2006-09-21

申请号：US11353293

申请日：2006-02-13

Applicant: Roland Carlsson ,  Elisabeth Sonesson ,  Yvonne Stenberg ,  Leif Strandberg ,  Gideon Goldstein

Inventor： Roland Carlsson ,  Elisabeth Sonesson ,  Yvonne Stenberg ,  Leif Strandberg ,  Gideon Goldstein

IPC: C12Q1/68 ,  C12N5/06 ,  C12N5/16

CPC classification number: C07K16/1072 ,  C07K2317/92

Abstract: A single isolated antibody or antibody fragment thereof binds to multiple variant sequences within an epitope of HIV-1 Tat protein displayed in multiple strains and subtypes of HIV-1. This “pan-epitope” antibody is useful in therapeutic and prophylactic compositions and treatments of HIV-1 infection, regardless of strain. This pan-epitope antibody is useful in assays for the detection of levels of HIV-1 based on a measurement of the amount of Tat protein in a biological sample.

公开(公告)号：US20060019395A1

公开(公告)日：2006-01-26

申请号：US11135235

申请日：2005-05-23

Applicant: Wayne Marasco

Inventor： Wayne Marasco

IPC: C12N15/867

CPC classification number: C12N15/86 ,  A61K48/00 ,  C07K16/00 ,  C07K16/1072 ,  C07K16/18 ,  C07K16/2866 ,  C07K2317/21 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/035 ,  C07K2319/60 ,  C12N7/00 ,  C12N15/1037 ,  C12N2740/15043 ,  C12N2740/16043 ,  C12N2740/16045 ,  C12N2800/30 ,  C12N2810/6054 ,  C12N2810/6081 ,  C12N2830/003 ,  C12N2830/48 ,  C12N2830/50 ,  C12N2830/60 ,  C12N2840/203 ,  C12N2840/44 ,  G01N33/6854 ,  G01N33/6857

Abstract: The invention relates to compositions containing polynucleotide vectors capable of expressing a nucleic acid encoding a fusion polypeptide on the surface of a viral particle and/or a eukaryotic cell.

Abstract translation: 本发明涉及包含能够在病毒颗粒和/或真核细胞的表面上表达编码融合多肽的核酸的多核苷酸载体的组合物。

公开(公告)号：US20030206900A1

公开(公告)日：2003-11-06

申请号：US10460471

申请日：2003-06-13

Applicant: INSTITUT PASTEUR ,  Universite Pierre et Marie Curie

Inventor： Therese Ternynck ,  Alexandre Avrameas ,  Gerard Buttin ,  Stratis Avrameas ,  Marie-Francoise Saron ,  Bruno Blondel ,  Therese Couderc ,  Susan Michelson ,  Donato Zipeto

IPC: A61K039/395 ,  C07K016/44 ,  C12P021/02 ,  C12N005/06 ,  C12N015/85

CPC classification number: C07K16/1072 ,  A61K38/00 ,  A61K47/62 ,  A61K47/6883 ,  A61K2039/505 ,  C07K16/18 ,  C07K16/28 ,  C07K16/44 ,  C07K2317/565 ,  C07K2317/80 ,  C07K2319/00 ,  C12N2710/16111 ,  C12N2710/16611 ,  C12N2770/32611 ,  Y02A50/466

Abstract: The present invention provides novel polypeptides which can effectively penetrate into cells thereby transporting a substance of interest into the cells.

公开(公告)号：US20030194411A1

公开(公告)日：2003-10-16

申请号：US10213742

申请日：2002-08-06

Inventor： Arye Rubinstein ,  Barry R. Bloom ,  Yair Devash ,  Stanley J. Cryz

IPC: A61K039/21 ,  C07K007/08 ,  C07K007/06 ,  C07K005/00 ,  C07K016/00 ,  A61K038/00 ,  A61K038/04 ,  C07K017/00 ,  C07K007/00

CPC classification number: C07K14/005 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/57 ,  A61K2039/6037 ,  A61K2039/6068 ,  A61K2039/6081 ,  A61K2039/70 ,  C07K14/21 ,  C07K14/33 ,  C07K14/35 ,  C07K16/1063 ,  C07K16/1072 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16322 ,  C12N2740/16334

Abstract: The present invention provides for peptide conjugate compositions, methods of using the peptide conjugate compositions, and pharmaceutical compositions comprising the peptide conjugate compositions. The peptide conjugate compositions comprise peptides with amino acid sequences similar to the gp 120 principal neutralizing domain (PND) of HIV, gp41, and Nef (p27) of HIV and carriers which enhance immunogenicity. The peptide conjugate compositions of the present invention may comprise a multivalent cocktail of several different peptide conjugates. Also provided by present invention is a method for reducing the level of HIV titers in a mammal by administering to the mammal a peptide composition of the present invention in an amount effective to reduce the level of HIV titers. The peptide conjugate compositions of the present invention induce prolonged antibody response in serum, a high level of antibody in the mucosa, and the production of cytotoxic lymphocytes. The peptide conjugate compositions of the present invention also elicit neutralizing antibodies and decrease viral loads in a subject.

Abstract translation: 本发明提供了肽缀合物组合物，使用肽缀合物组合物的方法和包含肽缀合物组合物的药物组合物。 肽缀合物组合物包含具有类似于HIV的gp120主要中和结构域（PND），HIV，gp41和Nef（p27）以及增强免疫原性的载体的氨基酸序列的肽。 本发明的肽缀合物组合物可以包含若干不同肽缀合物的多价混合物。 本发明还提供了通过向哺乳动物施用有效降低HIV滴度水平的量的本发明的肽组合物来降低哺乳动物的HIV滴度水平的方法。 本发明的肽缀合物组合物诱导血清中延长的抗体应答，粘膜中的高水平抗体和细胞毒性淋巴细胞的产生。 本发明的肽缀合物组合物还引发中和抗体并降低受试者的病毒载量。

公开(公告)号：US06447778B1

公开(公告)日：2002-09-10

申请号：US09599286

申请日：2000-06-22

Applicant: Arye Rubinstein ,  Barry R. Bloom ,  Yair Devash ,  Stanley J. Cryz

Inventor： Arye Rubinstein ,  Barry R. Bloom ,  Yair Devash ,  Stanley J. Cryz

IPC: A61K3804

CPC classification number: C07K14/005 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/57 ,  A61K2039/6037 ,  A61K2039/6068 ,  A61K2039/6081 ,  A61K2039/70 ,  C07K14/21 ,  C07K14/33 ,  C07K14/35 ,  C07K16/1063 ,  C07K16/1072 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16322 ,  C12N2740/16334

Abstract: The present invention provides for peptide conjugate compositions, methods of using the peptide conjugate compositions, and pharmaceutical compositions comprising the peptide conjugate compositions. The peptide conjugate compositions comprise peptides with amino acid sequences similar to the gp120 principal neutralizing domain (PND) of HIV, gp41, and Nef (p27) of HIV and carriers which enhance immunogenicity. The peptide conjugate compositions of the present invention may comprise a multivalent cocktail of several different peptide conjugates. Also provided by present invention is a method for reducing the level of HIV titers in a mammal by administering to the mammal a peptide composition of the present invention in an amount effective to reduce the level of HIV titers. The peptide conjugate compositions of the present invention induce prolonged antibody response in serum, a high level of antibody in the mucosa, and the production of cytotoxic lymphocytes. The peptide conjugate compositions of the present invention also elicit neutralizing antibodies and decrease viral loads in a subject.

Abstract translation: 本发明提供了肽缀合物组合物，使用肽缀合物组合物的方法和包含肽缀合物组合物的药物组合物。 肽缀合物组合物包含具有类似于HIV的gp120主要中和结构域（PND），HIV，gp41和Nef（p27）以及增强免疫原性的载体的氨基酸序列的肽。 本发明的肽缀合物组合物可以包含若干不同肽缀合物的多价混合物。 本发明还提供了通过向哺乳动物施用有效降低HIV滴度水平的量的本发明的肽组合物来降低哺乳动物的HIV滴度水平的方法。 本发明的肽缀合物组合物诱导血清中延长的抗体应答，粘膜中的高水平抗体和细胞毒性淋巴细胞的产生。 本发明的肽缀合物组合物还引发中和抗体并降低受试者的病毒载量。