公开(公告)号：US20240344057A1

公开(公告)日：2024-10-17

申请号：US18647281

申请日：2024-04-26

申请人： Twist Bioscience Corporation

发明人： Jeremy LACKEY ,  David DODD

IPC分类号： C12N15/10 ,  C12N15/113

CPC分类号： C12N15/1093 ,  C12N15/102 ,  C12N15/113 ,  C12N2310/315 ,  C12N2320/33

摘要： Disclosed herein are methods and compositions comprising a polymerase and a phosphorylated nucleoside, wherein the polymerase and the nucleoside are covalently linked by a cleavable linker at the terminal phosphate group. Further disclosed herein are enzymatic polynucleotide synthesis using polymerase and nucleotide conjugation strategies.

公开(公告)号：US12056264B2

公开(公告)日：2024-08-06

申请号：US17938228

申请日：2022-10-05

申请人： Twist Bioscience Corporation

发明人： Bill James Peck

IPC分类号： G06F21/78 ,  C12N15/10

CPC分类号： G06F21/78 ,  C12N15/102 ,  C12N15/1027 ,  C12N15/1089 ,  G06F2212/1052 ,  G06F2212/402

摘要： Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Additionally, devices described herein for de novo synthesis of nucleic acids encoding information related to the original source information may be rigid or flexible material. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Also provided herein are methods and systems for efficient transfer of preselected polynucleotides from a storage structure for reading stored information.

公开(公告)号：US12018065B2

公开(公告)日：2024-06-25

申请号：US17242170

申请日：2021-04-27

申请人： Twist Bioscience Corporation

发明人： Aaron Sato ,  Qiang Liu ,  Tom Yuan

IPC分类号： C07K16/10 ,  C07K16/00 ,  A61K39/00

CPC分类号： C07K16/10 ,  C07K16/005 ,  A61K2039/505 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92

摘要： Provided herein are methods and compositions relating to libraries of optimized antibodies having nucleic acids encoding for an antibody comprising modified sequences. Libraries described herein comprise nucleic acids encoding SARS-CoV-2 or ACE2 antibodies. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US11970697B2

公开(公告)日：2024-04-30

申请号：US17504358

申请日：2021-10-18

申请人： Twist Bioscience Corporation

发明人： Jeremy Lackey ,  David Dodd

IPC分类号： C12N15/10 ,  C12N15/113

CPC分类号： C12N15/1093 ,  C12N15/102 ,  C12N15/113 ,  C12N2310/315 ,  C12N2320/33

摘要： Disclosed herein are methods and compositions comprising a polymerase and a phosphorylated nucleoside, wherein the polymerase and the nucleoside are covalently linked by a cleavable linker at the terminal phosphate group. Further disclosed herein are enzymatic polynucleotide synthesis using polymerase and nucleotide conjugation strategies.

公开(公告)号：US20240001325A1

公开(公告)日：2024-01-04

申请号：US18345869

申请日：2023-06-30

申请人： Twist Bioscience Corporation

发明人： Eugene P. MARSH ,  Pierre F. INDERMUHLE ,  Bill James PECK

IPC分类号： B01J19/00

CPC分类号： B01J19/0046 ,  B01J2219/00722 ,  B01J2219/00596 ,  B01J2219/00533 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00662 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00509 ,  B01J2219/00716 ,  B01J2219/00659

摘要： Devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Nano-scale devices allow for selective control over reaction conditions. Further, methods and devices described herein allow for the rapid construction of large libraries of highly accurate nucleic acids.

公开(公告)号：US11745159B2

公开(公告)日：2023-09-05

申请号：US17122988

申请日：2020-12-15

申请人： Twist Bioscience Corporation

发明人： Eugene P. Marsh ,  Pierre F. Indermuhle ,  Bill James Peck

IPC分类号： B01J19/00

CPC分类号： B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00509 ,  B01J2219/00533 ,  B01J2219/00596 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00716 ,  B01J2219/00722

摘要： Defined sequence RNA synthesis by 3′→5′ direction is now well established and currently in use for synthesis and development of vast variety of therapeutic grade RNA and Si RNA etc. A number of such synthetic RNA requires a modification or labeling of 3′-end of an oligonucleotide. The synthesis of 3′-end modified RNA requiring lipophilic, long chain ligands or chromophores, using 3′→5′ synthesis methodology is challenging, requires corresponding solid support and generally results in low coupling efficiency and lower purity of the final oligonucleotide in general because of large amount of truncated sequences containing desired hydrophobic modification. We have approached this problem by developing reverse RNA monomer phosphoramidites for RNA synthesis in 5′→3′-direction. They lead to very clean oligonucleotide synthesis allowing for introduction of various modifications at the 3′-end.

公开(公告)号：US20230257448A1

公开(公告)日：2023-08-17

申请号：US18149593

申请日：2023-01-03

申请人： Twist Bioscience Corporation

发明人： Aaron SATO

IPC分类号： C07K16/10 ,  A61P31/14

CPC分类号： C07K16/10 ,  A61P31/14 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/35 ,  C07K2317/92 ,  A61K2039/545

摘要： Provided herein are methods and compositions relating to improved bispecific antibodies capable of binding and neutralizing SARS-CoV-2 variants.

公开(公告)号：US20230193513A1

公开(公告)日：2023-06-22

申请号：US17937377

申请日：2022-09-30

申请人： Twist Bioscience Corporation

发明人： Ray TABIBIAZAR ,  Aaron SATO ,  Pankaj GARG ,  Qiang LIU ,  Fumiko AXELROD

IPC分类号： C40B40/08 ,  C07K16/28

CPC分类号： C40B40/08 ,  C07K16/2869 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/624 ,  C07K2317/92

摘要： Provided herein are methods and compositions relating to glucagon-like peptide-1 receptor (GLP1R) libraries having nucleic acids encoding for a scaffold comprising a GLP1R binding domain. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US20230192819A1

公开(公告)日：2023-06-22

申请号：US17820541

申请日：2022-08-17

申请人： Twist Bioscience Corporation

发明人： Aaron SATO ,  Qiang LIU ,  Mary MATHIEU ,  Ana G. LUJAN HERNANDEZ

IPC分类号： C07K16/10 ,  A61P31/14

CPC分类号： C07K16/10 ,  A61P31/14 ,  A61K2039/545 ,  C07K2317/92 ,  C07K2317/565 ,  C07K2317/569

摘要： Provided herein are methods and compositions relating to libraries of optimized antibodies having nucleic acids encoding for an antibody comprising modified sequences. Libraries described herein comprise nucleic acids encoding SARS-CoV-2 or ACE2 antibodies. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US20230127969A1

公开(公告)日：2023-04-27

申请号：US18047228

申请日：2022-10-17

申请人： Twist Bioscience Corporation

发明人： Jeremy LACKEY ,  William BANYAI

IPC分类号： C12Q1/6869

摘要： Provided herein are compositions, devices, systems and methods for polynucleotide sequencing. Further provided are devices comprising surfaces for continuous sequencing. The compositions, devices, systems and methods described herein provide improved storage, density, and retrieval of biomolecule-based information.