公开(公告)号：US20080030738A1

公开(公告)日：2008-02-07

申请号：US11838909

申请日：2007-08-15

申请人： BENGT IVARSSON

发明人： BENGT IVARSSON

IPC分类号： G01N21/55

CPC分类号： G01B11/0625 ,  G01N21/552 ,  G01N21/553 ,  G01N2021/212 ,  G01N2021/215

摘要： A method of examining thin layer structures on a surface for differences in respect of optical thickness, which method comprises the steps of: irradiating the surface with light so that the light is internally or externally reflected at the surface; imaging the reflected light on a first two-dimensional detector; sequentially or continuously scanning the incident angle and/or wavelength of the light over an angular and/or wavelength range; measuring the intensities of light reflected from different parts of the surface and impinging on different parts of the detector, at least a number of incident angles and/or wavelengths, the intensity of light reflected from each part of the surface for each angle and/or wavelength depending on the optical thickness of the thin layer structure thereon; and determining from the detected light intensities at the different light incident angles and/or wavelengths an optical thickness image of the thin layer structures on the surface. According to the invention, part of the light reflected at said surface is detected on a second detector to determine the incident angle or wavelength of the polarized light irradiating the surface. An apparatus for carrying out the method is also disclosed.

摘要翻译： 一种检查表面上薄层结构相对于光学厚度差异的方法，该方法包括以下步骤：用光照射表面，使得光在表面内部或外部反射; 对第一二维检测器上的反射光进行成像; 在角度和/或波长范围内顺序地或连续地扫描光的入射角和/或波长; 测量从表面的不同部分反射并撞击检测器的不同部分的光的强度，至少多个入射角和/或波长，从每个角度的每个部分表面反射的光的强度和/或 波长取决于其上的薄层结构的光学厚度; 并且根据检测到的不同光入射角的光强度和/或波长确定表面上的薄层结构的光学厚度图像。 根据本发明，在第二检测器上检测在所述表面反射的光的一部分，以确定照射表面的偏振光的入射角或波长。 还公开了一种用于执行该方法的装置。

公开(公告)号：US20070016378A1

公开(公告)日：2007-01-18

申请号：US11452102

申请日：2006-06-12

申请人： Karl Andersson

发明人： Karl Andersson

IPC分类号： G06F19/00 ,  G06G7/48

CPC分类号： G16B20/00

摘要： A method of determining affinity for a molecular binding interaction from measured steady state binding data comprises the steps of: a) providing a plurality of experimental binding data sets for the binding interaction between two chemical species, wherein each data set includes binding data measured at multiple time points during an association phase and a dissociation phase of the interaction, b) selecting from the plurality of experimental binding data sets a plurality of binding data measured at a defined time point at or near the end of the association phase as representing steady state binding data, c) subjecting each data set to a quality control which comprises estimating the reliability of the steady state binding data by evaluating other binding data of the same data set, d) excluding each data set which is estimated in step c) to contain unreliable steady state binding data, and e) determining the affinity from the steady state binding data of remaining data sets.

摘要翻译： 从测量的稳态结合数据确定与分子结合相互作用的亲和力的方法包括以下步骤：a）提供用于两种化学物质之间的结合相互作用的多个实验结合数据集，其中每个数据集包括在多个测量的结合数据 在相关阶段和相互作用的解离阶段的时间点，b）从多个实验结合数据集合中选择在关联阶段的结束处或附近的规定的时间点测量的多个结合数据表示稳态结合 数据，c）对每个数据集进行质量控制，其包括通过评估相同数据集的其他绑定数据来估计稳态绑定数据的可靠性，d）排除在步骤c）中估计的每个数据集以包含不可靠 稳态绑定数据，以及e）从剩余数据集的稳态绑定数据确定亲和度。

公开(公告)号：US20060072115A1

公开(公告)日：2006-04-06

申请号：US11234829

申请日：2005-09-23

申请人： Bengt Ivarsson

发明人： Bengt Ivarsson

IPC分类号： G01N21/55

CPC分类号： G01B11/0625 ,  G01N21/552 ,  G01N21/553 ,  G01N2021/212 ,  G01N2021/215

摘要： A method of examining thin layer structures on a surface for differences in respect of optical thickness, which method comprises the steps of: irradiating the surface with light so that the light is internally or externally reflected at the surface; imaging the reflected light on a first two-dimensional detector; sequentially or continuously scanning the incident angle and/or wavelength of the light over an angular and/or wavelength range; measuring the intensities of light reflected from different parts of the surface and impinging on different parts of the detector, at at least a number of incident angles and/or wavelengths, the intensity of light reflected from each part of the surface for each angle and/or wavelength depending on the optical thickness of the thin layer structure thereon; and determining from the detected light intensities at the different light incident angles and/or wavelengths an optical thickness image of the thin layer structures on the surface. According to the invention, part of the light reflected at said surface is detected on a second detector to determine the incident angle or wavelength of the polarized light irradiating the surface. An apparatus for carrying out the method is also disclosed.

公开(公告)号：US20040248213A1

公开(公告)日：2004-12-09

申请号：US10815166

申请日：2004-03-31

申请人： Biacore AB

发明人： Robert Karlsson ,  Pascale Richalet-Secordel

IPC分类号： C12Q001/68 ,  G01N033/53 ,  G01N033/567 ,  G01N033/542

CPC分类号： G01N33/5005 ,  G01N33/543 ,  Y10S435/973

摘要： The present invention relates to a method for analysing a cell sample for cell surface-bound or intracellularly bound analytes by providing an array of immobilised specific binding agents for a set of different ligands, where each ligand is specific to a respective cell surface-bound or intracellularly bound analyte, and using the array and the set of ligands in an inhibition type or a direct type assay format to determine cell surface-bound or intracellularly bound analytes in the cell sample. The invention also relates to assay kits for cell characterization.

摘要翻译： 本发明涉及通过提供用于一组不同配体的固定化特异性结合剂的阵列分析细胞表面结合或细胞内结合的分析物的细胞样品的方法，其中每个配体对相应的细胞表面结合或特异性结合 细胞内结合的分析物，并使用阵列和该组配体以抑制型或直接型测定形式来确定细胞样品中细胞表面结合或细胞内结合的分析物。 本发明还涉及用于细胞表征的测定试剂盒。

公开(公告)号：US20020128593A1

公开(公告)日：2002-09-12

申请号：US10001246

申请日：2001-11-01

申请人： Biacore AB

发明人： Stefan Sjolander ,  Thord Hansson

IPC分类号： A61B017/20

CPC分类号： G01N35/1097 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2200/0689 ,  B01L2300/0874 ,  B01L2400/0655 ,  F16K7/126 ,  F16K7/17 ,  G01N21/05 ,  G01N21/553 ,  G01N2021/0346 ,  Y10T29/49405 ,  Y10T29/49417 ,  Y10T29/49425 ,  Y10T29/49426 ,  Y10T29/49428 ,  Y10T137/87893

摘要： The present invention discloses a valve integrally associated with a microfluidic transport assembly that is useful for regulating the flow of a liquid sample through an analytical instrument such as, for example, a biosensor. The valve integrally associated with a microfluidic liquid transport assembly, includes: a first rigid layer having substantially planar and opposing first and second surfaces; a second rigid layer having substantially planar and opposing third and fourth surfaces, the third surface of the second rigid layer being substantially coplanar and integrally bonded to the second surface of the first rigid layer; a first passageway defined by a groove, the groove being along the second surface of the first rigid layer and bounded by the third surface of the second rigid layer, the first passageway being adapted to flow a liquid sample therethrough, a valve seat having a substantially planar plateau surface, the valve seat being within the passageway and integrally connected to the first rigid layer such that the plateau surface is substantially planar to and interposed between the first and second surfaces of the first rigid layer; and a flexible membrane opposite the valve seat and integrally associated with a first membrane through hole of the second rigid layer, the flexible membrane having a passageway surface that is either (i) substantially coplanar to the second surface of the first rigid layer when the valve is in an open position, or (ii) bulged with a central portion thereof being substantially coplanar to the plateau surface of the valve seat when the valve is in a closed position. The present invention is also directed to methods of manufacturing of the same.

摘要翻译： 本发明公开了一种与微流体输送组件整体相关联的阀，其可用于通过诸如生物传感器的分析仪器来调节液体样品的流动。 与微流体液体输送组件整体相关联的阀包括：具有基本平坦且相对的第一和第二表面的第一刚性层; 第二刚性层，其具有基本平坦且相对的第三和第四表面，所述第二刚性层的第三表面基本共面并整体地结合到所述第一刚性层的第二表面; 由凹槽限定的第一通道，所述凹槽沿着所述第一刚性层的第二表面并且由所述第二刚性层的第三表面限定，所述第一通道适于使液体样品流过其中，所述阀座具有基本上 平台平台表面，阀座在通道内并且与第一刚性层一体地连接，使得平台表面基本上平面并插入在第一刚性层的第一和第二表面之间; 以及与所述阀座相对并且与所述第二刚性层的第一膜通孔整体相关联的柔性膜，所述柔性膜具有通道表面，所述通道表面（i）当所述阀（i）基本上与所述第一刚性层的第二表面共面时， 处于打开位置，或（ii）当阀处于关闭位置时，其中心部分与阀座的平台表面基本上共面膨胀。 本发明还涉及其制造方法。

公开(公告)号：US6008893A

公开(公告)日：1999-12-28

申请号：US273615

申请日：1999-03-22

申请人： Hakan Roos ,  Kjell Magnusson

发明人： Hakan Roos ,  Kjell Magnusson

IPC分类号： G01N33/543 ,  G01N35/08 ,  G01N35/10 ,  G01N21/01

CPC分类号： G01N35/085

摘要： Systems and methods for reversibly and controllably flowing a first and second fluid adjacent to one or more discrete sensing surfaces of a biosensor are disclosed herein. The systems utilize a flow-cell conduit adapted to flow the first and second fluids in either a first or second flow direction and in a manner such that the first and second fluids separately contact the one or more discrete sensing surfaces of the biosensor. The flow-cell conduit is operatively connected to both a two-fluid conduit and a single-fluid conduit via a valveless juncture. The system also comprises a selector valve for reversing the flow direction of the fluid within the flow-cell conduit.

摘要翻译： 本文公开了用于可逆地和可控地流动邻近生物传感器的一个或多个离散感测表面的第一和第二流体的系统和方法。 该系统利用流动池导管，其适于使第一和第二流体沿第一或第二流动方向流动，并且使得第一和第二流体分别接触生物传感器的一个或多个离散的感测表面。 流动池导管经由无阀接头可操作地连接到双流体导管和单流体导管两者。 该系统还包括用于反转流动池导管内的流体的流动方向的选择阀。

公开(公告)号：US5922594A

公开(公告)日：1999-07-13

申请号：US809617

申请日：1997-03-25

申请人： Stefan Lof.ang.s

发明人： Stefan Lof.ang.s

IPC分类号： B01D71/06 ,  G01N21/27 ,  G01N33/543 ,  G01N33/553 ,  G01Q80/00 ,  C12M1/34

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  G01N33/5432 ,  G01N33/54373 ,  G01N33/553 ,  G01N2610/00 ,  Y10S977/788 ,  Y10T436/104165

摘要： Methods are disclosed for producing a substrate surface supporting a continuous planar bilayer lipid membrane by covalently binding a plurality of micellar of vesicle liposomes, optionally comprising a membrane protein or other biologically active membrane-bound component, to a substrate surface supporting a self-assembled monolayer (SAM) of essentially straight long chain molecules. In one embodiment, the micellar or vesicle liposomes covantly bind to hydrophilic spacer molecules attached to the functional groups of the self-assembled monolayer.

摘要翻译： PCT No.PCT / SE95 / 01099 Sec。 371日期1997年3月25日 102（e）1997年3月25日PCT PCT 1995年9月26日PCT公布。 出版物WO96 / 10178 日本公开1996年4月4日公开了用于制备支持连续平面双层脂质膜的底物表面的方法，该基质表面通过共价结合多个胶束的囊泡脂质体，任选地包含膜蛋白或其他生物活性的膜结合组分， 基本上是直链长链分子的自组装单层（SAM）。 在一个实施方案中，胶束或囊泡脂质体与附着于自组装单层的官能团的亲水性间隔分子有明显的结合。

公开(公告)号：US20060134800A1

公开(公告)日：2006-06-22

申请号：US11295161

申请日：2005-12-06

申请人： Magnus Malmqvist ,  Hakan Roos ,  Stefan Sjolander ,  Mattias Tidare ,  Hakan Sjodin ,  Ralph Stalberg

发明人： Magnus Malmqvist ,  Hakan Roos ,  Stefan Sjolander ,  Mattias Tidare ,  Hakan Sjodin ,  Ralph Stalberg

IPC分类号： G01N1/10

CPC分类号： G01N21/553 ,  B01J2219/00511 ,  B01J2219/00572 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00734 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0877 ,  B82Y30/00 ,  G01N21/05 ,  G01N21/648 ,  G01N2021/0346 ,  G01N2021/058 ,  Y10S366/01 ,  Y10S366/03 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

摘要： Methods and devices are provided for controlling a fluid flow over a sensing surface within a flow cell. The methods employ laminar flow techniques to position a fluid flow over one or more discrete sensing areas on the sensing surface of the flow cell. Such methods permit selective sensitization of the discrete sensing areas, and provide selective contact of the discrete sensing areas with a sample fluid flow. Immobilization of a ligand upon the discrete sensing area, followed by selective contact with an analyte contained within the sample fluid flow, allows analysis by a wide variety of techniques. Sensitized sensing surfaces, and sensor devices and systems are also provided.

公开(公告)号：US20060094060A1

公开(公告)日：2006-05-04

申请号：US11249038

申请日：2005-10-11

申请人： Tanja Jarhede ,  Per Kjellin ,  Anita Larsson ,  Hans Sjobom

发明人： Tanja Jarhede ,  Per Kjellin ,  Anita Larsson ,  Hans Sjobom

IPC分类号： G01N33/53 ,  B05D3/00

CPC分类号： G01N33/548 ,  B82Y30/00 ,  G01N2800/52 ,  Y10S436/809

摘要： A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of providing a solid support having a hydrogel coating with a plurality of binding elements, coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, whereby the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel. Also the use of the reactive surface in analysis, such as immunogenicity assays, is disclosed.

摘要翻译： 公开了一种制备蛋白质抗性活性固体支持物表面的方法。 该方法包括以下步骤：提供具有多个结合元件的水凝胶涂层的固体支持物，通过结合元件的第一部分将蛋白质抗性化合物偶联至水凝胶，以及通过经由 结合元件的第二部分，其中蛋白质抗性化合物和至少一种结合剂共同固定在水凝胶上。 还公开了分析中的反应性表面的使用，例如免疫原性测定法。

公开(公告)号：US06999175B2

公开(公告)日：2006-02-14

申请号：US10766696

申请日：2004-01-27

申请人： Bengt Ivarsson

发明人： Bengt Ivarsson

IPC分类号： G01N21/55 ,  G01N11/28

CPC分类号： G01B11/0625 ,  G01N21/552 ,  G01N21/553 ,  G01N2021/212 ,  G01N2021/215

摘要： A method of examining thin layer structures on a surface for differences in respect of optical thickness, which method comprises the steps of: irradiating the surface with light so that the light is internally or externally reflected at the surface; imaging the reflected light on a first two-dimensional detector; sequentially or continuously scanning the incident angle and/or wavelength of the light over an angular and/or wavelength range; measuring the intensities of light reflected from different parts of the surface and impinging on different parts of the detector, at at least a number of incident angles and/or wavelengths, the intensity of light reflected from each part of the surface for each angle and/or wavelength depending on the optical thickness of the thin layer structure thereon; and determining from the detected light intensities at the different light incident angles and/or wavelengths an optical thickness image of the thin layer structures on the surface. According to the invention, part of the light reflected at said surface is detected on a second detector to determine the incident angle or wavelength of the polarized light irradiating the surface. An apparatus for carrying out the method is also disclosed.