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公开(公告)号：US11780863B2
公开(公告)日：2023-10-10
申请号：US16183583
申请日：2018-11-07
IPC分类号： C07F9/12 , C07H15/203 , C07H17/02 , C07H15/207 , C07F9/6561 , C07F9/6518 , C07F9/6558 , C07D209/14 , C07H15/26 , C07F9/58 , C07F9/6503 , C12Q1/42 , G01N33/58 , C07D403/06 , C07F9/6533 , C07F9/06
CPC分类号： C07F9/6561 , C07D209/14 , C07D403/06 , C07F9/06 , C07F9/12 , C07F9/58 , C07F9/6518 , C07F9/65031 , C07F9/65335 , C07F9/65583 , C07H15/203 , C07H15/207 , C07H15/26 , C07H17/02 , C12Q1/42 , G01N33/581
摘要： Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.
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2.发明申请公开(公告)号：US20210341466A1
公开(公告)日：2021-11-04
申请号：US17318541
申请日：2021-05-12
IPC分类号： G01N33/533 , G01N33/58 , C09B23/08 , C09B11/24 , G01N33/532
摘要： Disclosed herein are novel chromogenic conjugates, the conjugates comprising at least two detectable moieties.
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公开(公告)号：US20210221833A1
公开(公告)日：2021-07-22
申请号：US17149911
申请日：2021-01-15
发明人： Yuri Belosludtsev , Traci D. DeGeer , Wendy J. French , Junshan Hao , Brian D. Kelly , Adrian E. Murillo , Nathan W. Polaske
IPC分类号： C07F9/6558 , G01N33/58 , G01N33/542 , G01N33/573
摘要： Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for enabling the detection of targets located proximally to each other in a sample.
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公开(公告)号：US20150267262A1
公开(公告)日：2015-09-24
申请号：US14728833
申请日：2015-06-02
发明人： Chol S. Yun , Brian D. Kelly , Julia Ashworth-Sharpe , Christopher Bieniarz , Pascal Bamford , Adrian E. Murillo
IPC分类号： C12Q1/68 , C07D487/04
CPC分类号： G01N33/5308 , B82Y15/00 , C07D487/04 , C09B57/08 , C09B69/10 , C09B69/109 , C12Q1/6816 , C12Q1/6886 , C12Q2600/158 , G01N33/588 , C12Q2563/107 , C12Q2563/155
摘要： Embodiments of a system, method, and kit for visualizing a nucleus are disclosed. A tissue sample is pretreated with a protease to permeabilize the nucleus, and then incubated with a nanoparticle/DNA-binding moiety conjugate. The DNA-binding moiety includes at least one DNA-binding molecule. The conjugate binds to DNA within the nucleus, and the nanoparticle is visualized, thereby visualizing the nucleus. Computer and image analysis techniques are used to evaluate nuclear features such as chromosomal distribution, ploidy, shape, size, texture features, and/or contextual features. The method may be used in combination with other multiplexed tests on the tissue sample, including fluorescence in situ hybridization. Kits for performing the method include a protease enzyme composition, a nanoparticle/DNA-binding moiety conjugate, and a reaction buffer.
摘要翻译： 公开了用于可视化核的系统，方法和试剂盒的实施例。 用蛋白酶预处理组织样品以使细胞核透化，然后与纳米颗粒/ DNA结合部分缀合物一起温育。 DNA结合部分包括至少一个DNA结合分子。 该结合物与细胞核内的DNA结合，并且纳米颗粒被可视化，从而可视化细胞核。 计算机和图像分析技术用于评估核特征，如染色体分布，倍性，形状，大小，纹理特征和/或语境特征。 该方法可以与组织样品上的其他复用测试结合使用，包括荧光原位杂交。 用于进行该方法的试剂盒包括蛋白酶组合物，纳米颗粒/ DNA结合部分缀合物和反应缓冲液。
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公开(公告)号：US11971337B2
公开(公告)日：2024-04-30
申请号：US17493477
申请日：2021-10-04
发明人： James Grille , Brian D. Kelly , Jerome Kosmeder, II , Eric May , Noemi Sebastiao , Pamela Wirth
IPC分类号： G01N1/30 , A61K49/00 , C01B15/01 , C01B21/08 , C07C55/02 , C07C55/22 , C07C55/24 , C12N9/99 , G01N33/52
CPC分类号： G01N1/30 , C01B15/01 , C01B21/08 , C07C55/02 , C07C55/22 , C07C55/24 , C12N9/99 , G01N33/52 , A61K49/001 , G01N2001/302 , G01N2333/902 , G01N2333/908 , G01N2333/916
摘要： Disclosed are compositions and methods for inactivating one or more enzymes in a biological sample.
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公开(公告)号：US11860167B2
公开(公告)日：2024-01-02
申请号：US17586996
申请日：2022-01-28
发明人： Brian D. Kelly , Nathan W. Polaske
IPC分类号： G01N33/58 , C07F9/02 , C07F9/12 , C07F9/553 , C07F9/6524 , C07F9/6518 , C07F9/6509
CPC分类号： G01N33/582 , C07F9/12 , C07F9/5535 , C07F9/6518 , C07F9/6524 , C07F9/650947
摘要： Applicants have developed an amplification system and methodology for IHC and ISH staining that utilizes “click chemistry” to covalently bind reporter molecules to tissue.
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公开(公告)号：US11345719B2
公开(公告)日：2022-05-31
申请号：US16284098
申请日：2019-02-25
发明人： Yuri Belosludtsev , Traci D. DeGeer , Wendy J. French , Junshan Hao , Brian D. Kelly , Adrian E. Murillo , Nathan W. Polaske
IPC分类号： C07F9/6558 , G01N33/58 , G01N33/542 , G01N33/573
摘要： Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for enabling the detection of targets located proximally to each other in a sample.
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8.发明申请公开(公告)号：US20210055285A1
公开(公告)日：2021-02-25
申请号：US17081523
申请日：2020-10-27
IPC分类号： G01N33/533 , G01N33/58 , G01N33/532 , C09B11/24 , C09B23/08
摘要： Disclosed herein are novel chromogenic conjugates, the conjugates comprising at least two detectable moieties.
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公开(公告)号：US10775281B2
公开(公告)日：2020-09-15
申请号：US15483589
申请日：2017-04-10
IPC分类号： G01N1/30 , G01N33/532 , G01N33/535 , C12Q1/28 , G01N33/543 , G01N33/58
摘要： Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.
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10.发明申请公开(公告)号：US20190187130A1
公开(公告)日：2019-06-20
申请号：US16226427
申请日：2018-12-19
IPC分类号： G01N33/533 , G01N33/58 , C09B11/24 , C09B23/08
CPC分类号： G01N33/533 , C09B11/24 , C09B23/083 , G01N33/532 , G01N33/582 , G01N33/583
摘要： Disclosed herein are novel chromogenic conjugates, the conjugates comprising at least two detectable moieties.
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