公开(公告)号：US09792693B2

公开(公告)日：2017-10-17

申请号：US14776501

申请日：2014-03-13

申请人： Ventana Medical Systems, Inc.

发明人： Pascal Bamford ,  Srinivas Chukka ,  Lou Dietz ,  Ronald T. Kurnik ,  Bikash Sabata ,  Anindya Sarkar ,  Olcay Sertel

IPC分类号： G06T7/00 ,  G01J3/44 ,  G01N21/64 ,  G06T7/11

CPC分类号： G06T7/10 ,  G01J3/4406 ,  G01N21/6458 ,  G01N2021/6421 ,  G06T7/11 ,  G06T2207/10064

摘要： Processing of images acquired via fluorescence microscopy by identifying broadband and other undesired signals from the component signals of a scanned image, and processing selected regions of the image that are known to contain signals of interest, thereby extracting or identifying desired signals while subtracting undesired signals. One or more broadband signals are recognized by their unique signature and ubiquitous dispersion through the image. Regions of the scanned image may be tagged as consisting of predominantly broadband signals and are ignored during a spectral unmixing process. The remaining regions of the image, or selected regions of the image known to contain desired signals, may be unmixed, and the plurality of reference spectra subtracted from the components to extract or identify the target signals. The set of target signals may be refined by eliminating known or obvious sources of noise by, for instance, being compared to known or ideal sets of signals from similar materials.

公开(公告)号：US10521644B2

公开(公告)日：2019-12-31

申请号：US14372673

申请日：2013-01-29

申请人： Ventana Medical Systems, Inc.

发明人： Pascal Bamford ,  Srinivas Chukka ,  Jim F. Martin ,  Anindya Sarkar ,  Olcay Sertel ,  Ellen Suzue ,  Harshal Varangaonkar

IPC分类号： G06K9/00 ,  G06T7/00 ,  C12Q1/6813 ,  G06T7/90 ,  G06K9/46

摘要： A computer-based specimen analyzer (10) is configured to detect a level of expression of genes in a cell sample by detecting dots that represent differently stained genes and chromosomes in a cell. The color of the stained genes and the chromosomes is enhanced and filtered to produce a dot mask that defines areas in the image that are genes, chromosomes, or non-genetic material. Metrics are determined for the dots and/or pixels in the image of the cell in areas corresponding to the dots. The metrics are fed to a classifier that separates genes from chromosomes. The results of the classifier are counted to estimate the expression level of genes in the tissue samples.

公开(公告)号：US20160131583A1

公开(公告)日：2016-05-12

申请号：US14892527

申请日：2014-05-28

申请人： VENTANA MEDICAL SYSTEMS, INC.

发明人： Pascal Bamford ,  Lou Dietz ,  Elizabeth Little

IPC分类号： G01N21/64 ,  G01N33/50 ,  G02B21/34

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/5005 ,  G01N2021/6419 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/062 ,  G01N2458/00 ,  G02B21/16 ,  G02B21/34 ,  G02B27/56

摘要： An imaging system (100) is capable of detecting of tissue situated on a microscope slide (180). The imaging system (100) includes a light source (150), an image capturing device (160) including a camera, and an imaging lens (170). The light source (150) directs light (154) towards one or more of the edges of the slide (180) such that the light (154) undergoes total internal reflection between a surface of the slide (180) and a coverslip (182) carried by the slide (180). The light (154) has a wavelength or waveband designed to stimulate one or more specimens carried on the slide (180). The imaging lens (170) is positioned to direct radiation emitted from the tissue and/or fluorophore onto the camera. The image capturing device (160) can capture an image of the whole slide (180) or a portion thereof.

摘要翻译： 成像系统（100）能够检测位于显微镜载片（180）上的组织。 成像系统（100）包括光源（150），包括相机的图像捕获装置（160）和成像透镜（170）。 光源（150）将光（154）引向滑动件（180）的一个或多个边缘，使得光（154）在滑动件（180）的表面和盖玻片（182）之间经历全内反射， 由幻灯片（180）承载。 光（154）具有被设计成刺激载玻片（180）上的一个或多个样本的波长或波段。 成像透镜（170）被定位成将从组织和/或荧光团发射的辐射引导到相机上。 图像捕获装置（160）可以捕获整个幻灯片（180）或其一部分的图像。

公开(公告)号：US20150267262A1

公开(公告)日：2015-09-24

申请号：US14728833

申请日：2015-06-02

申请人： Ventana Medical Systems, Inc.

发明人： Chol S. Yun ,  Brian D. Kelly ,  Julia Ashworth-Sharpe ,  Christopher Bieniarz ,  Pascal Bamford ,  Adrian E. Murillo

IPC分类号： C12Q1/68 ,  C07D487/04

CPC分类号： G01N33/5308 ,  B82Y15/00 ,  C07D487/04 ,  C09B57/08 ,  C09B69/10 ,  C09B69/109 ,  C12Q1/6816 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N33/588 ,  C12Q2563/107 ,  C12Q2563/155

摘要： Embodiments of a system, method, and kit for visualizing a nucleus are disclosed. A tissue sample is pretreated with a protease to permeabilize the nucleus, and then incubated with a nanoparticle/DNA-binding moiety conjugate. The DNA-binding moiety includes at least one DNA-binding molecule. The conjugate binds to DNA within the nucleus, and the nanoparticle is visualized, thereby visualizing the nucleus. Computer and image analysis techniques are used to evaluate nuclear features such as chromosomal distribution, ploidy, shape, size, texture features, and/or contextual features. The method may be used in combination with other multiplexed tests on the tissue sample, including fluorescence in situ hybridization. Kits for performing the method include a protease enzyme composition, a nanoparticle/DNA-binding moiety conjugate, and a reaction buffer.

摘要翻译： 公开了用于可视化核的系统，方法和试剂盒的实施例。 用蛋白酶预处理组织样品以使细胞核透化，然后与纳米颗粒/ DNA结合部分缀合物一起温育。 DNA结合部分包括至少一个DNA结合分子。 该结合物与细胞核内的DNA结合，并且纳米颗粒被可视化，从而可视化细胞核。 计算机和图像分析技术用于评估核特征，如染色体分布，倍性，形状，大小，纹理特征和/或语境特征。 该方法可以与组织样品上的其他复用测试结合使用，包括荧光原位杂交。 用于进行该方法的试剂盒包括蛋白酶组合物，纳米颗粒/ DNA结合部分缀合物和反应缓冲液。

公开(公告)号：US20140078286A1

公开(公告)日：2014-03-20

申请号：US14112354

申请日：2012-05-04

申请人： Ventana Medical Systems, Inc.

发明人： Pascal Bamford ,  Michael Otter ,  Ronald T. Kurnik

IPC分类号： G02B21/36

CPC分类号： G02B21/361 ,  G06K9/0014 ,  G06K2009/4657

摘要： A method and system for spectral demultiplexing of fluorescent species, such as quantum dots, conjugated with a biological tissue. The process of demultiplexing involves a non-liner regression based on curve-fitting of estimated spectra of the quantum dots and confidence intervals describing the parameters of such fitting curve for typical quantum dots.

摘要翻译： 用于与生物组织缀合的荧光物质如量子点的光谱解复用的方法和系统。 解复用的过程涉及基于量子点的估计光谱的曲线拟合和描述典型量子点的拟合曲线的参数的置信区间的非线性回归。

公开(公告)号：US20140377753A1

公开(公告)日：2014-12-25

申请号：US14372673

申请日：2013-01-29

申请人： Ventana Medical Systems, Inc.

发明人： Pascal Bamford ,  Srinivas Chukka ,  Jim F. Martin ,  Anindya Sarkar ,  Olcay Sertel ,  Ellen Suzue ,  Harshal Varangaonkar

IPC分类号： G06K9/00 ,  G06T7/40 ,  C12Q1/68

CPC分类号： G06K9/00147 ,  C12Q1/6813 ,  G06K9/4652 ,  G06T7/0012 ,  G06T7/90 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A computer-based specimen analyzer (10) is configured to detect a level of expression of genes in a cell sample by detecting dots that represent differently stained genes and chromosomes in a cell. The color of the stained genes and the chromosomes is enhanced and filtered to produce a dot mask that defines areas in the image that are genes, chromosomes, or non-genetic material. Metrics are determined for the dots and/or pixels in the image of the cell in areas corresponding to the dots. The metrics are fed to a classifier that separates genes from chromosomes. The results of the classifier are counted to estimate the expression level of genes in the tissue samples.

摘要翻译： 基于计算机的样本分析器（10）被配置为通过检测代表细胞中不同染色的基因和染色体的点来检测细胞样品中基因的表达水平。 染色基因和染色体的颜色被增强和过滤以产生定义图像中基因，染色体或非遗传物质区域的点掩模。 针对与点对应的区域中的单元格的图像中的点和/或像素确定度量。 将度量提供给将染色体分离基因的分类器。 计算分类器的结果以估计组织样品中基因的表达水平。