知搜-全球专利检索

  1. Login
  2. Sign Up

Search Results

21 records (took 0.038 seconds)

    PmST3 enzyme for chemoenzymatic synthesis of alpha-2-3-sialosides
    4.
    发明授权
    PmST3 enzyme for chemoenzymatic synthesis of alpha-2-3-sialosides 有权

    公开(公告)号：US09783838B2

    公开(公告)日：2017-10-10

    申请号：US14356376

    申请日：2012-11-07

    Applicant: The Regents of the University of California

    Inventor： Xi Chen ,  Vireak Thon ,  Hai Yu

    IPC: C12P21/00 ,  C12P19/18 ,  C12P19/26 ,  C12P19/44 ,  C12N9/10

    CPC classification number: C12P21/005 ,  C12N9/1051 ,  C12N9/1081 ,  C12P19/18 ,  C12P19/26 ,  C12P19/44

    Abstract: The present invention provides novel methods for preparing glycosylated molecules such as oligosaccharides, glycolipids, and glycoproteins/peptides. Novel sialyltransferases are also disclosed. The method includes forming a reaction mixture containing an acceptor molecule, a donor substrate having a sugar moiety and a nucleotide, and a sialyltransferase selected from PmST3 (SEQ ID NO:7) and certain variants thereof. The reaction mixture is formed under conditions sufficient to transfer the sugar moiety from the donor substrate to the acceptor molecule, thereby forming the glycosylated molecule. In some embodiments, the acceptor molecule is selected from a natural product, an oligosaccharide, a glycoprotein, and a glycolipid. In some embodiments, the donor substrate is formed via conversion of a suitable hexosamine derivative to a cytidine 5′-monophosphate(CMP)-sialic acid in a one-pot reaction mixture containing asialic acid aldolase and a CMP-sialic acid synthetase.

    PmST3 ENZYME FOR CHEMOENZYMATIC SYNTHESIS OF ALPHA-2-3-SIALOSIDES
    7.
    发明申请
    PmST3 ENZYME FOR CHEMOENZYMATIC SYNTHESIS OF ALPHA-2-3-SIALOSIDES 有权
    PmST3用于ALPHA-2-3脂质体的化学合成的酶

    公开(公告)号：US20140349339A1

    公开(公告)日：2014-11-27

    申请号：US14356376

    申请日：2012-11-07

    Applicant: The Regents of the University of California

    Inventor： Xi Chen ,  Vireak Thon ,  Hai Yu

    IPC: C12P21/00 ,  C12N9/10

    CPC classification number: C12P21/005 ,  C12N9/1051 ,  C12N9/1081 ,  C12P19/18 ,  C12P19/26 ,  C12P19/44

    Abstract: The present invention provides novel methods for preparing glycosylated molecules such as oligosaccharides, glycolipids, and glycoproteins/peptides. Novel sialyltransferases are also disclosed. The method includes forming a reaction mixture containing an acceptor molecule, a donor substrate having a sugar moiety and a nucleotide, and a sialyltransferase selected from PmST3 (SEQ ID NO:7) and certain variants thereof. The reaction mixture is formed under conditions sufficient to transfer the sugar moiety from the donor substrate to the acceptor molecule, thereby forming the glycosylated molecule. In some embodiments, the acceptor molecule is selected from a natural product, an oligosaccharide, a glycoprotein, and a glycolipid. In some embodiments, the donor substrate is formed via conversion of a suitable hexosamine derivative to a cytidine 5′- monophosphate(CMP)-sialic acid in a one-pot reaction mixture containing asialic acid aldolase and a CMP-sialic acid synthetase.

    Abstract translation: 本发明提供了制备糖基化分子例如寡糖，糖脂和糖蛋白/肽的新方法。 还公开了新的唾液酸转移酶。 该方法包括形成含有受体分子，具有糖部分的供体底物和核苷酸以及选自PmST3（SEQ ID NO：7）及其某些变体的唾液酸转移酶的反应混合物。 反应混合物在足以将糖部分从供体底物转移到受体分子的条件下形成，从而形成糖基化分子。 在一些实施方案中，受体分子选自天然产物，寡糖，糖蛋白和糖脂。 在一些实施方案中，通过在含有唾液酸醛缩酶和CMP-唾液酸合成酶的一锅反应混合物中将合适的己糖胺衍生物转化为胞苷5'-单磷酸（CMP） - 唾液酸形成供体底物。

Patent Agency Ranking