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    Glucose dehydrogenase
    1.
    发明授权
    Glucose dehydrogenase 有权
    葡萄糖脱氢酶

    公开(公告)号：US08394615B2

    公开(公告)日：2013-03-12

    申请号：US12811735

    申请日：2008-12-26

    申请人： Hiroshi Aiba ,  Yoshiaki Nishiya ,  Tadayuki Imanaka ,  Haruyuki Atomi

    发明人： Hiroshi Aiba ,  Yoshiaki Nishiya ,  Tadayuki Imanaka ,  Haruyuki Atomi

    IPC分类号： C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/54 ,  C07H21/04 ,  C07K1/00

    CPC分类号： C12Y101/05002 ,  C12N9/0006 ,  C12Q1/32 ,  C12Q1/54

    摘要： The invention provides a glucose dehydrogenase that is an extremely stable enzyme having a thermostability of 80° C. or more, and that does not substantially act upon saccharides other than glucose (e.g., having a reactivity of less than 3% with respect to maltose, galactose, and xylose). The invention also provides a method for producing such an enzyme, and a composition for quantifying glucose using such an enzyme.

    摘要翻译： 本发明提供了一种葡萄糖脱氢酶，其是具有80℃或更高的热稳定性的非常稳定的酶，并且基本上不对葡萄糖以外的糖起作用（例如，相对于麦芽糖具有小于3％的反应性， 半乳糖和木糖）。 本发明还提供了生产这种酶的方法，以及使用这种酶定量葡萄糖的组合物。

    Method of targeted gene disruption, genome of hyperthermostable bacterium and genome chip using the same
    2.
    发明申请
    Method of targeted gene disruption, genome of hyperthermostable bacterium and genome chip using the same 审中-公开
    靶向基因破坏的方法，超嗜热细菌的基因组和使用其的基因组芯片

    公开(公告)号：US20060248617A1

    公开(公告)日：2006-11-02

    申请号：US10526324

    申请日：2003-08-29

    申请人： Tadayuki Imanaka ,  Haruyuki Atomi

    发明人： Tadayuki Imanaka ,  Haruyuki Atomi

    IPC分类号： G06F19/00 ,  C12Q1/68 ,  C07H21/02 ,  C12N15/82 ,  A01H1/00

    CPC分类号： C07K14/195 ,  C12Q1/02 ,  G01N2333/195 ,  G01N2500/10

    摘要： It is intended to provide an efficient and sure gene targeting method embodied at an arbitrary position in the genome of an organism and a kit therefor. It is also intended to provide a method for targeted-disruption of an arbitrary gene in the genome of an organism which comprises: 1) the step of providing the whole sequencial data of the genome of the organism; 2) the step of selecting at least one arbitrary region in the sequence; 3) the step of providing a vector containing a sequence homologous with the region selected above and a marker gene; 4) the step of transforming the organism by the vector; and 5) the step of providing the organism under such conditions as allowing homologous recombination. Moreover, the genome of a hyperthermostable bacterium and its array are provided.

    摘要翻译： 旨在提供一种在生物体的基因组中任意位置实施的有效且可靠的基因靶向方法及其用于其的试剂盒。 还旨在提供一种用于靶向生物体基因组中任意基因的靶向破坏的方法，其包括：1）提供生物体基因组的整个序列数据的步骤; 2）选择序列中的至少一个任意区域的步骤; 3）提供含有与上述选择区域同源的序列的载体和标记基因的步骤; 4）通过载体转化生物体的步骤; 和5）在允许同源重组的条件下提供生物体的步骤。 此外，提供了超热稳定细菌的基因组及其阵列。

    NOVEL GLUCOSE DEHYDROGENASE
    3.
    发明申请
    NOVEL GLUCOSE DEHYDROGENASE 有权
    新葡萄糖脱氢酶

    公开(公告)号：US20110020851A1

    公开(公告)日：2011-01-27

    申请号：US12811735

    申请日：2008-12-26

    申请人： Hiroshi Aiba ,  Yoshiaki Nishiya ,  Tadayuki Imanaka ,  Haruyuki Atomi

    发明人： Hiroshi Aiba ,  Yoshiaki Nishiya ,  Tadayuki Imanaka ,  Haruyuki Atomi

    IPC分类号： C12Q1/54 ,  C12N9/04 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12Y101/05002 ,  C12N9/0006 ,  C12Q1/32 ,  C12Q1/54

    摘要： The invention provides a glucose dehydrogenase that is an extremely stable enzyme having a thermostability of 80° C. or more, and that does not substantially act upon saccharides other than glucose (e.g., having a reactivity of less than 3% with respect to maltose, galactose, and xylose). The invention also provides a method for producing such an enzyme, and a composition for quantifying glucose using such an enzyme.

    摘要翻译： 本发明提供了一种葡萄糖脱氢酶，其是具有80℃或更高的热稳定性的非常稳定的酶，并且基本上不对葡萄糖以外的糖起作用（例如，相对于麦芽糖具有小于3％的反应性， 半乳糖和木糖）。 本发明还提供了生产这种酶的方法，以及使用这种酶定量葡萄糖的组合物。

    DNA encoding a thermostable DNA polymerase
    4.
    发明授权
    DNA encoding a thermostable DNA polymerase 失效
    编码热稳定DNA聚合酶的DNA

    公开(公告)号：US06187573B1

    公开(公告)日：2001-02-13

    申请号：US09363095

    申请日：1999-07-30

    申请人： Tadayuki Imanaka ,  Masahiro Takagi ,  Masaaki Morikawa

    发明人： Tadayuki Imanaka ,  Masahiro Takagi ,  Masaaki Morikawa

    IPC分类号： C12P2104

    CPC分类号： C12Q1/686 ,  C12N9/1252 ,  C12Q2521/101

    摘要： A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3′-5′ exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.

    摘要翻译： 提供了具有短反应时间和高保真度的核酸扩增酶。 本发明的酶是具有至少30个碱基/秒的核酸延伸速率和3'-5'核酸外切酶活性的热稳定的DNA聚合酶。 还提供了用于扩增核酸的方法和试剂盒。

    Modified thermostable DNA polymerase
    6.
    发明授权
    Modified thermostable DNA polymerase 有权
    修饰的热稳定DNA聚合酶

    公开(公告)号：US07422882B2

    公开(公告)日：2008-09-09

    申请号：US09852922

    申请日：2001-05-10

    申请人： Toshihiro Kuroita ,  Masao Kitabayashi ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Bunsei Kawakami ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    发明人： Toshihiro Kuroita ,  Masao Kitabayashi ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Bunsei Kawakami ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    IPC分类号： C12N9/12 ,  C12P19/34

    CPC分类号： C12N9/1252

    摘要： An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3′-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.

    摘要翻译： 本发明的目的是提供具有增强的扩增效率和/或聚合酶链反应（PCR）中的改进的保真度的热稳定性DNA聚合酶，并提供其生产方法。 更具体地说，本发明提供了热稳定的DNA聚合酶，其中在X 1，X 2，X 3，X 4， 序列（D：天冬氨酸，E：谷氨酸，H：组氨酸，X 1，X 2，X 3和X 3， 由SEQ ID NO：1区域内的DX1E序列组成的4个氨基酸长度肽与邻近所述谷氨酸（E）的4个氨基酸长度的肽组成，所述热稳定性DNA聚合酶具有3' -5'核酸外切酶活性，组氨酸（H）已被另一种氨基酸取代。

    Method for low-temperature culture of microorganism
    8.
    发明申请
    Method for low-temperature culture of microorganism 审中-公开
    微生物低温培养方法

    公开(公告)号：US20050032159A1

    公开(公告)日：2005-02-10

    申请号：US10738922

    申请日：2003-12-16

    申请人： Eita Ichige ,  Hisashi Semba ,  Tadayuki Imanaka

    发明人： Eita Ichige ,  Hisashi Semba ,  Tadayuki Imanaka

    IPC分类号： C12N15/09 ,  C12N1/00 ,  C12N1/20 ,  C12N1/21 ,  C12N1/36 ,  C12R1/19 ,  C12P21/06 ,  C12P13/04

    CPC分类号： C12N1/20 ,  C12N1/36

    摘要： A method for high-density culture of a microorganism is proposed. This method is characterized by adjusting the cultivation temperature to a level lower than the optimum temperature for growth thereby causing the specific growth rate of the microorganism to reach a level of not more than 20% of the specific growth rate at the optimum temperature for growth. By this method of culture, it is made possible to culture the microorganism at a very high density. Further, this method of culture enables a useful substance, particularly an active type recombinant protein, to be produced in a large amount at a very low cost with high efficiency.

    摘要翻译： 提出了一种微生物高密度培养的方法。 该方法的特征在于将培养温度调节至低于生长的最适温度的水平，从而使得微生物的比生长速率在生长的最适温度下达到不超过比生长速率的20％的水平。 通过这种培养方法，可以以非常高的密度培养微生物。 此外，这种培养方法能够以非常低的成本高效率地生产大量的有用物质，特别是活性型重组蛋白质。

    Dna synthesis promoters dna polymerase-associated factors and utilization thereof
    9.
    发明申请
    Dna synthesis promoters dna polymerase-associated factors and utilization thereof 有权
    Dna合成启动子dna聚合酶相关因子及其利用

    公开(公告)号：US20050069887A1

    公开(公告)日：2005-03-31

    申请号：US10495581

    申请日：2002-11-14

    申请人： Masao Kitabayashi ,  Toshihiro Kuroita ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Masanori Oka ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    发明人： Masao Kitabayashi ,  Toshihiro Kuroita ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Masanori Oka ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    IPC分类号： C07K14/195 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C07K14/195 ,  C12N9/1252 ,  C12Q1/6846 ,  C12Q2527/125 ,  C12Q2521/101

    摘要： A composition for enhancing synthesis of DNA comprising an anion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions, in particular a salt of a dicarboxylic acid. Further enhanced DNA synthesis promoting effects can be achieved by using, together with the anion-supplying substance, at least one compound selected from the group consisting of dimethylsulfoxide and compounds represented by the following formula (1): R2—CH2—NR1xHy  (1) wherein R1 is an alkyl group having 1 to 3 carbon atoms, R2 is a substituent selected from the group consisting of the following (a) and (b): (a) oxygen and (b) radicals represented by the formula wherein R4 is methyl, hydrogen or forms a pyrrolidine ring when combined with R1, R5 is —CO2H or —SO3H, and n is an integer from 0 to 2, x is an integer from 1 to 3 and y is an integer from 0 to 2, provided that x plus y equals 3. The present invention further provides a thermostable DNA polymerase-related factor derived from Thermococcus species, which has an activity to promote the DNA synthesis activity of DNA polymerase or bind to DNA polymerase.

    摘要翻译： 一种用于增强DNA合成的组合物，其包含在促进DNA合成反应中有效促进DNA合成的阴离子供给物质，特别是二羧酸的盐。 通过与阴离子供给物质一起使用至少一种选自二甲基亚砜和下式（1）表示的化合物的化合物，可以实现进一步增强的DNA合成促进效果：R 2 -CH 2 -NR <1> xHy（1）其中R 1是具有1至3个碳原子的烷基，R 2是选自以下（a）和（b）的取代基：（a）氧 和（b）由下式表示的基团其中R 4是甲基，氢或当与R 1一起形成吡咯烷环时，R 5是-CO 2 H或-SO 3 H，n是0至 2，x是1至3的整数，y是0至2的整数，条件是x加y等于3.本发明还提供了源自Thermococcus物种的热稳定DNA聚合酶相关因子，其具有活性 促进DNA聚合酶的DNA合成活性或与DNA聚合酶结合。