知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

4 条记录 (耗时 0.018 秒)

    Modified thermostable DNA polymerase
    1.
    发明授权
    Modified thermostable DNA polymerase 有权
    修饰的热稳定DNA聚合酶

    公开(公告)号：US07422882B2

    公开(公告)日：2008-09-09

    申请号：US09852922

    申请日：2001-05-10

    申请人： Toshihiro Kuroita ,  Masao Kitabayashi ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Bunsei Kawakami ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    发明人： Toshihiro Kuroita ,  Masao Kitabayashi ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Bunsei Kawakami ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    IPC分类号： C12N9/12 ,  C12P19/34

    CPC分类号： C12N9/1252

    摘要： An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3′-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.

    摘要翻译： 本发明的目的是提供具有增强的扩增效率和/或聚合酶链反应（PCR）中的改进的保真度的热稳定性DNA聚合酶，并提供其生产方法。 更具体地说，本发明提供了热稳定的DNA聚合酶，其中在X 1，X 2，X 3，X 4， 序列（D：天冬氨酸，E：谷氨酸，H：组氨酸，X 1，X 2，X 3和X 3， 由SEQ ID NO：1区域内的DX1E序列组成的4个氨基酸长度肽与邻近所述谷氨酸（E）的4个氨基酸长度的肽组成，所述热稳定性DNA聚合酶具有3' -5'核酸外切酶活性，组氨酸（H）已被另一种氨基酸取代。

    Dna synthesis promoters dna polymerase-associated factors and utilization thereof
    3.
    发明申请
    Dna synthesis promoters dna polymerase-associated factors and utilization thereof 有权
    Dna合成启动子dna聚合酶相关因子及其利用

    公开(公告)号：US20050069887A1

    公开(公告)日：2005-03-31

    申请号：US10495581

    申请日：2002-11-14

    申请人： Masao Kitabayashi ,  Toshihiro Kuroita ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Masanori Oka ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    发明人： Masao Kitabayashi ,  Toshihiro Kuroita ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Masanori Oka ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    IPC分类号： C07K14/195 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C07K14/195 ,  C12N9/1252 ,  C12Q1/6846 ,  C12Q2527/125 ,  C12Q2521/101

    摘要： A composition for enhancing synthesis of DNA comprising an anion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions, in particular a salt of a dicarboxylic acid. Further enhanced DNA synthesis promoting effects can be achieved by using, together with the anion-supplying substance, at least one compound selected from the group consisting of dimethylsulfoxide and compounds represented by the following formula (1): R2—CH2—NR1xHy  (1) wherein R1 is an alkyl group having 1 to 3 carbon atoms, R2 is a substituent selected from the group consisting of the following (a) and (b): (a) oxygen and (b) radicals represented by the formula wherein R4 is methyl, hydrogen or forms a pyrrolidine ring when combined with R1, R5 is —CO2H or —SO3H, and n is an integer from 0 to 2, x is an integer from 1 to 3 and y is an integer from 0 to 2, provided that x plus y equals 3. The present invention further provides a thermostable DNA polymerase-related factor derived from Thermococcus species, which has an activity to promote the DNA synthesis activity of DNA polymerase or bind to DNA polymerase.

    摘要翻译： 一种用于增强DNA合成的组合物，其包含在促进DNA合成反应中有效促进DNA合成的阴离子供给物质，特别是二羧酸的盐。 通过与阴离子供给物质一起使用至少一种选自二甲基亚砜和下式（1）表示的化合物的化合物，可以实现进一步增强的DNA合成促进效果：R 2 -CH 2 -NR <1> xHy（1）其中R 1是具有1至3个碳原子的烷基，R 2是选自以下（a）和（b）的取代基：（a）氧 和（b）由下式表示的基团其中R 4是甲基，氢或当与R 1一起形成吡咯烷环时，R 5是-CO 2 H或-SO 3 H，n是0至 2，x是1至3的整数，y是0至2的整数，条件是x加y等于3.本发明还提供了源自Thermococcus物种的热稳定DNA聚合酶相关因子，其具有活性 促进DNA聚合酶的DNA合成活性或与DNA聚合酶结合。

    Waterproof material and method for applying it
    4.
    发明授权
    Waterproof material and method for applying it 失效
    防水材料及其应用方法

    公开(公告)号：US5935683A

    公开(公告)日：1999-08-10

    申请号：US838261

    申请日：1997-04-17

    申请人： Takashi Iiyama ,  Eiichi Yamasaki ,  Takashi Nakano ,  Yoshikazu Ishida

    发明人： Takashi Iiyama ,  Eiichi Yamasaki ,  Takashi Nakano ,  Yoshikazu Ishida

    IPC分类号： B29C70/50 ,  B32B27/04 ,  B32B27/20 ,  E04D5/10 ,  B32B3/10

    CPC分类号： E04D5/10 ,  B29C70/508 ,  B32B27/04 ,  B32B27/20 ,  Y10T428/24124 ,  Y10T428/24355 ,  Y10T428/2913

    摘要： A waterproof material is disclosed herein which is obtainable by laminating many sheets of a photo-setting type fiber reinforced thermosetting resin composition comprising a resin composition containing a thermosetting resin, a photopolymerization initiator and a thickener, and a fiber reinforced material, or by laminating plural sheets of a synthetic polymer, and a waterproof application method using the waterproof material is also disclosed herein. According to the present invention, the waterproof layer can be formed on the surface of a civil engineering works or a building in a short time of about 30 minutes by a few steps without polluting a working environment. Furthermore, the waterproof layer, needless to say, has water resistance, weathering resistance, anticorrosion and substrate adhesion. In addition, it is further excellent in follow-up properties to cracks of the substrate, and walking on the waterproof layer is possible.

    摘要翻译： 本文公开了一种防水材料，其可以通过层压多张光固化型纤维增强热固性树脂组合物而获得，该组合物包含含有热固性树脂，光聚合引发剂和增稠剂的树脂组合物和纤维增强材料，或通过层压多个 合成聚合物的片材以及使用防水材料的防水涂布方法也在本文中公开。 根据本发明，防水层可以在约30分钟的短时间内几个步骤形成在土木工程或建筑物的表面上，而不会污染工作环境。 此外，不用说，防水层具有耐水性，耐候性，耐腐蚀性和基材附着性。 此外，对于基板的裂纹的追随特性进一步优异，并且在防水层上行走是可能的。