发明授权
- 专利标题： Modified thermostable DNA polymerase
- 专利标题（中）： 修饰的热稳定DNA聚合酶
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申请号： US09852922申请日： 2001-05-10
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公开(公告)号： US07422882B2公开(公告)日： 2008-09-09
- 发明人: Toshihiro Kuroita , Masao Kitabayashi , Yoshikazu Ishida , Hideyuki Komatsubara , Yoshiaki Nishiya , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka
- 申请人： Toshihiro Kuroita , Masao Kitabayashi , Yoshikazu Ishida , Hideyuki Komatsubara , Yoshiaki Nishiya , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka
- 申请人地址： JP Osaka
- 专利权人： Toyo Boseki Kabushiki Kaisha
- 当前专利权人： Toyo Boseki Kabushiki Kaisha
- 当前专利权人地址： JP Osaka
- 代理机构： Kenyon & Kenyon LLP
- 优先权： JP2000-138796 20000511
- 主分类号： C12N9/12
- IPC分类号： C12N9/12 ; C12P19/34
摘要：
An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3′-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.
公开/授权文献
- US20020076768A1 Modified thermostable DNA polymerase 公开/授权日：2002-06-20
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