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34 条记录 (耗时 0.021 秒)

    Protein having fructosyl valyl histidine oxidase activity, modified protein, and use of the protein or the modified protein
    1.
    发明授权
    Protein having fructosyl valyl histidine oxidase activity, modified protein, and use of the protein or the modified protein 有权
    具有果糖基缬氨酰基组氨酸氧化酶活性的蛋白质，修饰蛋白质，以及蛋白质或修饰蛋白质的使用

    公开(公告)号：US08993255B2

    公开(公告)日：2015-03-31

    申请号：US13123052

    申请日：2009-10-06

    申请人： Rie Hirao ,  Masao Kitabayashi ,  Yoshiaki Nishiya ,  Hiroki Ishida ,  Yoji Hata

    发明人： Rie Hirao ,  Masao Kitabayashi ,  Yoshiaki Nishiya ,  Hiroki Ishida ,  Yoji Hata

    IPC分类号： C12Q1/00 ,  C12Q1/26 ,  C12N11/00 ,  C12N9/02 ,  G01N33/72 ,  C12N9/06

    CPC分类号： G01N33/723 ,  C12N9/0022 ,  C12Q1/26

    摘要： The present invention provides: a protein having a fructosyl amino acid oxidase activity which protein is useful for measurement of a glycosylated protein (particularly, glycosylated hemoglobin); a modified protein thereof; and use of the protein or the modified protein. The protein of the present invention is, for example, a fructosyl valyl histidine oxidase derived from Phaeosphaeria nodorum, the fructosyl valyl histidine oxidase having excellent thermal stability and substrate specificity and also having a small Km value to fructosyl valyl histidine. This allows a glycosylated protein measuring reagent to be stored in a long time and measurement accuracy of the glycosylated protein measuring reagent to be improved.

    摘要翻译： 本发明提供：具有果糖基氨基酸氧化酶活性的蛋白质，该蛋白质可用于糖基化蛋白质（特别是糖基化血红蛋白）的测定。 其改性蛋白质; 并使用蛋白质或修饰的蛋白质。 本发明的蛋白质是例如来源于花粉球形的果糖基缬氨酰基组氨酸氧化酶，具有优异的热稳定性和底物特异性的果糖基缬氨酰基组氨酸氧化酶，并且对于果糖基缬氨酰基组氨酸也具有小的Km值。 这样可以长时间地存储糖基化蛋白质测定试剂，改善糖基化蛋白质测定试剂的测定精度。

    Modified creatinine amide hydrolase having improved affinity for substrate, and reagent composition for determination of creatinine
    2.
    发明授权
    Modified creatinine amide hydrolase having improved affinity for substrate, and reagent composition for determination of creatinine 有权
    具有改善的对底物的亲和性的改性肌酐酰胺水解酶和用于测定肌酸酐的试剂组合物

    公开(公告)号：US07816116B2

    公开(公告)日：2010-10-19

    申请号：US12297572

    申请日：2007-04-20

    申请人： Rie Nagai ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    发明人： Rie Nagai ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    IPC分类号： C12N9/00 ,  C12N9/78 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12Q1/34 ,  C12N9/80

    摘要： To overcome disadvantages of a known creatinine amide hydrolase, and provide a creatinine amide hydrolase having improved affinity for creatinine or having a decreased Km value for creatinine, and also provide a reagent composition for use in the determination of creatinine, which is adapted to an automated analysis apparatus and is excellent in accuracy, preciseness and economic efficiency.Disclosed is a modified creatinine amide hydrolase having improved affinity for a substrate compared to an unmodified one. Also disclosed is a creatinine determination reagent comprising the modified creatinine amide hydrolase, a creatinine amidino hydrolase, sarcosine oxidase and a reagent for detection of hydrogen peroxide.

    摘要翻译： 为了克服已知的肌酸酐酰胺水解酶的缺点，并提供对肌酸酐具有改善的亲和力或对肌酸酐具有降低的Km值的肌酸酐酰胺水解酶，并且还提供用于测定肌酸酐的试剂组合物，其适用于自动化 分析仪器，精度高，精度高，经济效益好。 公开了与未修饰的肌酸酐酰胺水解酶相比，对底物具有改善的亲和性。 还公开了包含改性肌酐酰胺水解酶，肌酸酐脒基水解酶，肌氨酸氧化酶和检测过氧化氢的试剂的肌酸酐测定试剂。

    Modified thermostable DNA polymerase
    4.
    发明授权
    Modified thermostable DNA polymerase 有权
    修饰的热稳定DNA聚合酶

    公开(公告)号：US07422882B2

    公开(公告)日：2008-09-09

    申请号：US09852922

    申请日：2001-05-10

    申请人： Toshihiro Kuroita ,  Masao Kitabayashi ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Bunsei Kawakami ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    发明人： Toshihiro Kuroita ,  Masao Kitabayashi ,  Yoshikazu Ishida ,  Hideyuki Komatsubara ,  Yoshiaki Nishiya ,  Bunsei Kawakami ,  Yoshihisa Kawamura ,  Tadayuki Imanaka

    IPC分类号： C12N9/12 ,  C12P19/34

    CPC分类号： C12N9/1252

    摘要： An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3′-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.

    摘要翻译： 本发明的目的是提供具有增强的扩增效率和/或聚合酶链反应（PCR）中的改进的保真度的热稳定性DNA聚合酶，并提供其生产方法。 更具体地说，本发明提供了热稳定的DNA聚合酶，其中在X 1，X 2，X 3，X 4， 序列（D：天冬氨酸，E：谷氨酸，H：组氨酸，X 1，X 2，X 3和X 3， 由SEQ ID NO：1区域内的DX1E序列组成的4个氨基酸长度肽与邻近所述谷氨酸（E）的4个氨基酸长度的肽组成，所述热稳定性DNA聚合酶具有3' -5'核酸外切酶活性，组氨酸（H）已被另一种氨基酸取代。

    Composition for measuring glucose having improved substrate specificity
    6.
    发明授权
    Composition for measuring glucose having improved substrate specificity 失效
    用于测量具有改善的底物特异性的葡萄糖的组合物

    公开(公告)号：US07381540B2

    公开(公告)日：2008-06-03

    申请号：US11393285

    申请日：2006-03-30

    申请人： Masao Kitabayashi ,  Hiroshi Aiba

    发明人： Masao Kitabayashi ,  Hiroshi Aiba

    IPC分类号： C12Q1/32

    CPC分类号： C12Q1/006 ,  G01N33/66 ,  G01N2333/904

    摘要： The present invention relates to a method for lowering activity with respect to maltose in glucose measurement comprising a step of reacting modified pyrroloquinoline quinone dependent glucose dehydrogenase subjected to amino acid sequence modification, wherein pyrroloquinoline quinone dependent glucose dehydrogenase is reacted in the presence of at least one type of substance selected from the group comprising succinic acid, malonic acid, glutaric acid, malic acid, phthalic acid, 2-ketoglutaric acid, 3,3-dimethylglutaric acid, pimeric acid, suberic acid, adipic acid, maleic acid, potassium chloride, ammonium chloride, diammonium hydrogen citrate, L-lysine, taurine, calcium glycerate, amino-n-butyric acid, sodium glycolate, sodium α-ketoglutarate, fumaric acid, glycine, glutamic acid, serine and citric acid.

    摘要翻译： 本发明涉及一种降低葡萄糖测定中的麦芽糖活性的方法，包括使经过氨基酸序列修饰的经修饰的吡咯并喹啉醌依赖性葡萄糖脱氢酶的反应步骤，其中吡咯并喹啉醌依赖性葡萄糖脱氢酶在至少一种 选自琥珀酸，丙二酸，戊二酸，苹果酸，邻苯二甲酸，2-酮戊二酸，3,3-二甲基戊二酸，多聚酸，辛二酸，己二酸，马来酸，氯化钾， 氯化铵，柠檬酸二铵，L-赖氨酸，牛磺酸，甘油酸钙，氨基正丁酸，乙醇酸钠，α-酮戊二酸钠，富马酸，甘氨酸，谷氨酸，丝氨酸和柠檬酸。

    MODIFIED FLAVIN ADENINE DINUCLEOTIDE-DEPENDENT GLUCOSE DEHYDROGENASE
    9.
    发明申请
    MODIFIED FLAVIN ADENINE DINUCLEOTIDE-DEPENDENT GLUCOSE DEHYDROGENASE 有权
    改性FLAVIN ADENINE DINUCLEOTIDE-Dependent GLUCOSE DEHYDROGENASE

    公开(公告)号：US20120171708A1

    公开(公告)日：2012-07-05

    申请号：US13421207

    申请日：2012-03-15

    申请人： Hiroshi KAWAMINAMI ,  Masao KITABAYASHI ,  Yoshiaki NISHIYA

    发明人： Hiroshi KAWAMINAMI ,  Masao KITABAYASHI ,  Yoshiaki NISHIYA

    IPC分类号： C12Q1/54 ,  C12N1/21 ,  C12N15/63 ,  C12N9/04 ,  C12N15/53

    CPC分类号： C12Q1/006 ,  C12N9/0006 ,  C12Q1/32 ,  C12Q1/54

    摘要： Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent.A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO: 2 or an amino acid sequence that has a 60% homology therewith, and that has an improved temperature dependency.

    摘要翻译： 提供了与公知的血糖传感器的酶相比，在实用方面更有利的酶，并且可以用于血糖水平测定试剂。 具有包含SEQ ID NO：2所示的氨基酸序列中的特定氨基酸的氨基酸序列的黄素腺嘌呤二核苷酸依赖型葡萄糖脱氢酶或与其具有60％同源性的氨基酸序列，并且具有改善的温度依赖性 。

    Modified flavin adenine dinucleotide dependent glucose dehydrogenase
    10.
    发明授权
    Modified flavin adenine dinucleotide dependent glucose dehydrogenase 有权
    改良黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶

    公开(公告)号：US07662600B2

    公开(公告)日：2010-02-16

    申请号：US11939348

    申请日：2007-11-13

    申请人： Hiroshi Kawaminami ,  Yuji Tsuji ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    发明人： Hiroshi Kawaminami ,  Yuji Tsuji ,  Masao Kitabayashi ,  Yoshiaki Nishiya

    IPC分类号： C12N9/04 ,  C12Q1/54 ,  C12P19/34 ,  C12N15/00 ,  C12P21/06 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C12N9/0006

    摘要： An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors.A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.

    摘要翻译： 本发明的目的是提供比用作血糖传感器的已知酶更可实用的用作测量血糖的试剂的酶。 与来自野生型FADGDH的FADGDH相比，具有比来自野生型FADGDH的FADGDH更好的热稳定性的改良的黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶（FADGDH），修饰的FADGDH优选来自真核生物，更优选丝状真菌，更优选来自真菌， 例如具有至少一个氨基酸被取代，缺失，插入或添加到具有序列表中SEQ ID No.2或46所示的氨基酸序列的FADGDH的一级结构的那些。