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    Method of labeling a target nucleic acid
    1.
    发明授权
    Method of labeling a target nucleic acid 有权
    标记靶核酸的方法

    公开(公告)号：US09145586B2

    公开(公告)日：2015-09-29

    申请号：US13904769

    申请日：2013-05-29

    Applicant: Samsung Electronics Co., Ltd.

    Inventor： Sea-hee Kim ,  Joo-won Rhee ,  Ko-bong Choi

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6844 ,  C12Q2521/131 ,  C12Q2525/173 ,  C12Q2533/101 ,  C12Q2563/107

    Abstract: Methods of labeling and detecting a target nucleic acid by incubating a target nucleic acid with a terminal transferase to extend a terminus of the target nucleic acid and provide an extended region; hybridizing the extended region of the target nucleic acid with a template polynucleotide having a nucleotide sequence complementary to the extended region to obtain a hybridization product; and incubating the hybridization product with a nucleic acid polymerase and either a deoxynucleotide triphosphate (dNTP) having a detectable label or nucleotide triphosphate (NTP) having a detectable label to further extend the extended target nucleic acid.

    Abstract translation: 通过将靶核酸与末端转移酶孵育以延伸靶核酸的末端并提供延伸区域来标记和检测靶核酸的方法; 将靶核酸的延伸区域与具有与延伸区域互补的核苷酸序列的模板多核苷酸杂交以获得杂交产物; 并用杂交产物与核酸聚合酶和具有可检测标记或具有可检测标记的核苷酸三磷酸（NTP）的脱氧核苷酸三磷酸（dNTP）孵育，以进一步扩展延伸的靶核酸。

    Polynucleotide and use thereof
    4.
    发明授权
    Polynucleotide and use thereof 有权
    多核苷酸及其用途

    公开(公告)号：US09157106B2

    公开(公告)日：2015-10-13

    申请号：US14051259

    申请日：2013-10-10

    Applicant: Samsung Electronics Co., Ltd.

    Inventor： Sea-hee Kim ,  Joo-won Rhee ,  Ko-bong Choi

    IPC: C12P19/34 ,  C12N15/00 ,  C12Q1/68 ,  C12N15/10

    CPC classification number: C12P19/34 ,  C12N15/00 ,  C12N15/10 ,  C12Q1/6844 ,  C12Q2525/301 ,  C12Q2531/125

    Abstract: Provided is a polynucleotide including, from the 3′ terminus of the polynucleotide to the 5′ terminus of the polynucleotide, a first region including a nucleotide sequence complementary to a nucleotide sequence of a portion of a target nucleic acid; a second region including a nucleotide sequence identical to a nucleotide sequence of a portion of the target nucleic acid; and a third region including a nucleotide sequence that self-hybridizes to form a stem-loop structure, and compositions, kits, and methods related thereto.

    Abstract translation: 本发明提供的多核苷酸包括从多核苷酸的3'末端到多核苷酸的5'末端的第一区域，其包含与靶核酸的一部分核苷酸序列互补的核苷酸序列; 第二区域，其包含与靶核酸的一部分的核苷酸序列相同的核苷酸序列; 以及包含自体杂交以形成茎 - 环结构的核苷酸序列的第三区域，以及与其相关的组合物，试剂盒和方法。

    Polynucleotide and use thereof
    6.
    发明授权
    Polynucleotide and use thereof 有权
    多核苷酸及其用途

    公开(公告)号：US09428798B2

    公开(公告)日：2016-08-30

    申请号：US14273790

    申请日：2014-05-09

    Applicant: Samsung Electronics Co., Ltd.

    Inventor： Sea-hee Kim ,  Joon-ho Kim ,  Joo-won Rhee ,  Sun-ok Jung ,  Ko-bong Choi ,  Kyu-youn Hwang

    IPC: C12Q1/68 ,  C07H21/02

    CPC classification number: C12Q1/6848 ,  C12Q2521/327 ,  C12Q2525/186 ,  C12Q2525/301

    Abstract: A polynucleotide comprising a first region the 5′ end of which is complementary to a portion of a target nucleic acid, a cleavable second region, a third region having a stem-loop structure, and a fourth region complementary to the 3′ end of the first region, and use of the polynucleotide, as well as a composition comprising two such polynucleotides each of which hybridize different strands of a double-stranded target nucleic acid, and methods and kits using the same for amplifying targets.

    Abstract translation: 一种多核苷酸，其包含其5'末端与靶核酸的一部分互补的第一区域，可切割第二区域，具有茎环结构的第三区域和与所述第三区域的3'末端互补的第四区域 第一区域和多核苷酸的使用，以及包含两个这样的多核苷酸的组合物，其各自与双链靶核酸的不同链杂交，以及将其用于扩增靶标的方法和试剂盒。

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