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    Compositions for amplification of RNA sequences using composite primers
    4.
    发明授权
    Compositions for amplification of RNA sequences using composite primers 有权
    使用复合引物扩增RNA序列的组合物

    公开(公告)号：US09181582B2

    公开(公告)日：2015-11-10

    申请号：US13922146

    申请日：2013-06-19

    Applicant: NUGEN TECHNOLOGIES, INC.

    Inventor： Nurith Kurn

    IPC: C07H21/04 ,  C12Q1/68

    CPC classification number: C12Q1/6853 ,  C12Q2531/119

    Abstract: The invention provides methods for isothermal amplification of RNA. The methods are particularly suitable for amplifying a plurality of RNA species in a sample. The methods employ a composite primer, a second primer and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In another aspect, the methods employ a single primer (which is a composite primer) and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In some embodiments, a transcription step is included to generate multiple copies of sense RNA of an RNA sequence of interest. The methods are useful for preparation of nucleic acid libraries and substrates for analysis of gene expression of cells in biological samples. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.

    Abstract translation: 本发明提供了RNA等温扩增的方法。 该方法特别适用于扩增样品中的多种RNA物种。 该方法采用复合引物，第二引物和链置换，以产生包含与感兴趣的RNA序列互补的序列的DNA产物的多个拷贝。 另一方面，该方法采用单一引物（其是复合引物）和链置换以产生包含与感兴趣的RNA序列互补的序列的DNA产物的多个拷贝。 在一些实施方案中，包括转录步骤以产生感兴趣的RNA序列的有义RNA的多个拷贝。 该方法可用于制备用于分析生物样品中细胞基因表达的核酸文库和底物。 本发明还提供了用于实施扩增方法的组合物和试剂盒，以及使用扩增产物的方法。

    COMPOSITIONS AND METHODS FOR NEGATIVE SELECTION OF NON-DESIRED NUCLEIC ACID SEQUENCES
    5.
    发明申请
    COMPOSITIONS AND METHODS FOR NEGATIVE SELECTION OF NON-DESIRED NUCLEIC ACID SEQUENCES 有权
    阴性选择非靶核酸序列的组合物和方法

    公开(公告)号：US20150299767A1

    公开(公告)日：2015-10-22

    申请号：US14390012

    申请日：2013-03-15

    Applicant: NUGEN TECHNOLOGIES, INC.

    Inventor： Christopher Armour ,  Doug Amorese ,  Bin Li ,  Nurith Kurn

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6844 ,  C12Q2525/131 ,  C12Q2525/191 ,  C12Q2521/301 ,  C12Q2521/531 ,  C12Q2537/159

    Abstract: The present invention provides methods, compositions and kits for the generation of next generation sequencing (NGS) libraries in which non-desired nucleic acid sequences have been depleted or substantially reduced. The methods, compositions and kits provided herein are useful, for example, for the production of libraries from total RNA with reduced ribosomal RNA and for the reduction of common mRNA species in expression profiling from mixed samples where the mRNAs of interest are present at low levels. The methods of the invention can be employed for the elimination of non-desired nucleic acid sequences in a sequence-specific manner, and consequently, for the enrichment of nucleic acid sequences of interest in a nucleic acid library.

    Abstract translation: 本发明提供用于产生下一代测序（NGS）文库的方法，组合物和试剂盒，其中非期望的核酸序列已经耗尽或显着降低。 本文提供的方法，组合物和试剂盒是有用的，例如用于从具有降低的核糖体RNA的总RNA生产文库，并且用于从感兴趣的mRNA以低水平存在的混合样品中降低表达谱中常见的mRNA种类 。 本发明的方法可用于以序列特异性方式消除非期望的核酸序列，并因此用于富集核酸文库中目的核酸序列。

    METHODS, COMPOSITIONS AND KITS FOR GENERATION OF STRANDED RNA OR DNA LIBRARIES
    6.
    发明申请
    METHODS, COMPOSITIONS AND KITS FOR GENERATION OF STRANDED RNA OR DNA LIBRARIES 审中-公开
    方法，用于生成条带RNA或DNA文库的组合物和试剂盒

    公开(公告)号：US20140274729A1

    公开(公告)日：2014-09-18

    申请号：US14030761

    申请日：2013-09-18

    Applicant: NuGEN Technologies, Inc.

    Inventor： Nurith Kurn ,  Bin Li

    IPC: B01J19/00

    CPC classification number: B01J19/0046

    Abstract: The invention provides methods and compositions, including kits, for the construction of directional nucleic acid libraries. The invention further provides methods and compositions for the amplification and sequencing of directional cDNA libraries.

    Abstract translation: 本发明提供了用于构建定向核酸文库的方法和组合物，包括试剂盒。 本发明还提供了用于扩增和测序定向cDNA文库的方法和组合物。

    Global Amplification Using a Randomly Primed Composite Primer
    7.
    发明申请
    Global Amplification Using a Randomly Primed Composite Primer 有权
    全面扩增使用随机引物复合引物

    公开(公告)号：US20140038236A1

    公开(公告)日：2014-02-06

    申请号：US13918636

    申请日：2013-06-14

    Applicant: NuGEN Technologies, Inc.

    Inventor： Nurith Kurn ,  Shenglong Wang

    IPC: C12P19/34

    CPC classification number: C12P19/34 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q2537/143 ,  C12Q2525/203 ,  C12Q2525/179

    Abstract: The invention relates to the field of polynucleotide amplification. More particularly, the invention provides methods, compositions and kits for amplification of (i.e., making multiple copies of) a multiplicity of different polynucleotide template sequences using a randomly primed RNA/DNA composite primer.

    Abstract translation: 本发明涉及多核苷酸扩增领域。 更具体地，本发明提供了使用随机引物的RNA / DNA复合引物扩增多个不同多核苷酸模板序列（即制备多个拷贝）的方法，组合物和试剂盒。

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