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    Nucleotide Sequence Encoding Homeobox-Leucine Zipper Protein HAT22 (HD-ZIP Protein 22) From Corchorus Olitorius and Corchorus Capsularis and Methods of Use
    1.
    发明申请
    Nucleotide Sequence Encoding Homeobox-Leucine Zipper Protein HAT22 (HD-ZIP Protein 22) From Corchorus Olitorius and Corchorus Capsularis and Methods of Use 审中-公开
    编码来自Corchorus Olitorius和Corchorus Capsularis的Homeobox-Leucine拉链蛋白HAT22（HD-ZIP蛋白22）的核苷酸序列和使用方法

    公开(公告)号：US20160333366A1

    公开(公告)日：2016-11-17

    申请号：US14781243

    申请日：2014-11-21

    Applicant: Maqsudul ALAM ,  BANGLADESH JUTE RESEARCH INSTITUTE

    Inventor： Maqsudul Alam ,  Mohammed Shahidul Islam ,  Borhan Ahmed ,  Mohammed Samiul Haque ,  Mohammed Monjurul Alarm

    IPC: C12N15/82 ,  C07K14/415

    CPC classification number: C12N15/8261 ,  C07K14/415 ,  Y02A40/146

    Abstract: The present invention relates to the isolated polynucleotide encoding homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22) from the plants Corchorus olitorius and Corchorus capsularis and corresponding polypeptide derived thereof. The present invention also relates to the plants having modulated expression of a nucleic acid encoding a homeobox-leucine zipper HAT22 polypeptide or a homologue thereof, which has the ability to modify, preferably to increase/enhance, the fiber length, plant height, and/or plant biomass. More specifically, the invention relates to polypeptides having homeobox-leucine zipper protein HAT22 activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. The present invention further provides vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22). The invention also provides methods for producing the said protein and methods for modifying the said protein in order to improve their desirable characteristics. The said protein of the invention can be used in a variety of ways, including increasing/enhancing the fiber length, height and biomass of plants and fiber yield.

    Abstract translation: 本发明涉及编码来自植物Corchorus olitorius和Corchorus capsularis的同源盒 - 亮氨酸拉链蛋白HAT22（HD-ZIP蛋白22）的分离的多核苷酸及其衍生的相应多肽。 本发明还涉及具有调节表达编码同源框 - 亮氨酸拉链HAT22多肽或其同源物的核酸的植物，其具有修饰，优选增加/增强纤维长度，植物高度和/ 或植物生物量。 更具体地，本发明涉及具有同源异构体 - 亮氨酸拉链蛋白HAT22活性的多肽，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 本发明还提供载体，表达构建体和宿主细胞，其包含同源异构体 - 亮氨酸拉链蛋白HAT22（HD-ZIP蛋白22）的核苷酸序列和/或组成。 本发明还提供了用于生产所述蛋白质的方法和用于改变所述蛋白质的方法，以便改善其所需特征。 本发明的所述蛋白质可以以多种方式使用，包括增加/增强植物的纤维长度，高度和生物量以及纤维产量。

    PECTIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF
    2.
    发明申请
    PECTIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 有权
    来自大麦酚酞的PECTIN降解酶及其用途

    公开(公告)号：US20150259667A1

    公开(公告)日：2015-09-17

    申请号：US14421729

    申请日：2013-08-15

    Applicant: Bangladesh Jute Research Institute ,  RAFIA HASINA

    Inventor： Maqsudul Alam ,  Mohammed S. Islam ,  Mohammed M. Hossen ,  Mohammed S. Haque ,  Mohammed M. Alam

    IPC: C12N9/88 ,  C12N9/18

    CPC classification number: C12N9/88 ,  C12N9/18 ,  C12N9/2405 ,  C12Y301/01011 ,  C12Y402/02002 ,  C12Y402/02023

    Abstract: The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina (“M. phaseolina”), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.

    Abstract translation: 本发明公开了分离的多核苷酸编码酶，该多核苷酸来自真菌巨噬细胞相容素（“M. phaseolina”），其负责降解果胶，并且其包含和/或由SEQ ID NO：1所示的核苷酸序列组成。 编号2，5，8，11，14，17，20，23，26，29，32，35，38，41，44，47，50，53，56，59，62，1,4,7 ，10,13,16,19,22,25,28,31,34,37,40,43,46,49,52,55,58和61，或这些序列的补体。 本发明还涉及由SEQ ID No.3,6,9,12,15,18,21,24,27,30,33,36,39,42,45， 48，51，54，57，60及63条; 包含该多核苷酸的重组基因构建体; 转化体和转基因真菌，其包含具有或具有增强的果胶降解酶生产的重组基因构建体。 本发明的多肽可以用于制造果汁，纺织品，纸浆和纸，咖啡，茶和油提取和果胶废水处理。

    Nucleotide sequence encoding homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22) from Corchorus olitorius and Corchorus capsularis and methods of use
    3.
    发明授权
    Nucleotide sequence encoding homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22) from Corchorus olitorius and Corchorus capsularis and methods of use 有权

    公开(公告)号：US10301639B2

    公开(公告)日：2019-05-28

    申请号：US14781243

    申请日：2014-11-21

    Applicant: Bangladesh Jute Research Institute ,  Rafia Hasina

    Inventor： Maqsudul Alam ,  Mohammed Shahidul Islam ,  Borhan Ahmed ,  Mohammed Samiul Haque ,  Mohammed Monjurul Alam

    IPC: C12N15/82 ,  C07K14/415

    Abstract: The present invention relates to the isolated polynucleotide encoding homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22) from the plants Corchorus olitorius and Corchorus capsularis and corresponding polypeptide derived thereof. The present invention also relates to the plants having modulated expression of a nucleic acid encoding a homeobox-leucine zipper HAT22 polypeptide or a homologue thereof, which has the ability to modify, preferably to increase/enhance, the fiber length, plant height, and/or plant biomass. More specifically, the invention relates to polypeptides having homeobox-leucine zipper protein HAT22 activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. The present invention further provides vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22). The invention also provides methods for producing the said protein and methods for modifying the said protein in order to improve their desirable characteristics. The said protein of the invention can be used in a variety of ways, including increasing/enhancing the fiber length, height and biomass of plants and fiber yield.

    CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF
    4.
    发明申请
    CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 有权
    纤维素和/或致细胞毒素降解大蒜素的酶及其用途

    公开(公告)号：US20150291945A1

    公开(公告)日：2015-10-15

    申请号：US14421774

    申请日：2013-08-15

    Applicant: Bangladesh Jute Research Institute

    Inventor： Maqsudul Alam ,  Mohammed S. Islam ,  Mohammed M. Hossen ,  Mohammed S. Haque ,  Mohammed M. Alam

    IPC: C12N9/24 ,  C12N9/38 ,  C12N9/88 ,  C12N9/34 ,  C12N9/42 ,  C12N9/26

    CPC classification number: C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12N9/2471 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/88 ,  C12Y302/01037 ,  C12Y302/01051 ,  C12Y302/01055 ,  C12Y302/01059 ,  C12Y302/01075 ,  C12Y302/01089 ,  C12Y302/0113 ,  C12Y302/01177

    Abstract: The present invention provides nucleotide sequences of Macrophomina phaseolina (“M. phaseolina”) that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a β-glucosidase, a a-glucosidase, a xylanase, a mannanse, a β-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a-fucosidases, a β-galactanase, an unsaturated β-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.

    Abstract translation: 本发明提供了编码具有纤维素分解活性的蛋白质/酶的Macrophomina phaseolina（“M. phaseolina”）的核苷酸序列，包括纤维素酶活性，内切葡聚糖酶，纤维二糖水解酶，葡糖苷酶，α-葡糖苷酶，木聚糖酶， 甘露聚糖，α-木糖苷酶，α-木糖苷酶，半乳糖苷酶，阿拉伯呋喃糖苷酶，α-岩藻糖苷酶，β-半乳糖苷酶，不饱和的 - 葡萄糖醛酸水解酶和/或寡聚酶活性。 也提供了包含和/或由该酶基因的核苷酸序列组成的载体，表达构建体和宿主细胞。 本发明还提供了用于生产酶的方法和用于改变酶的方法，以便改善其期望的特性。 本发明的酶可以多种但不限于制药，农业，食品和饲料加工，生物燃料，能源效率和工业环境。 这些酶也可用于将木质纤维素生物质完全水解成单糖，然后将其发酵成液体燃料和化学原料。

    Nucleotide Sequence Encoding Wuschel-Related Homeobox4 (WOX4) Protein From Corchorus Olitorius and Corchorus Capsularis and Methods of Use For Same
    10.
    发明申请
    Nucleotide Sequence Encoding Wuschel-Related Homeobox4 (WOX4) Protein From Corchorus Olitorius and Corchorus Capsularis and Methods of Use For Same 审中-公开
    编码来自Corchorus Olitorius和Corchorus Capsularis的Wuschel相关Homeobox4（WOX4）蛋白的核苷酸序列及其使用方法

    公开(公告)号：US20160369288A1

    公开(公告)日：2016-12-22

    申请号：US14781169

    申请日：2014-11-20

    Applicant: Maqsudul Alam

    Inventor： Maqsudul Alam

    IPC: C12N15/82 ,  C07K14/415

    CPC classification number: C12N15/8242 ,  C07K14/415 ,  C12N15/8218 ,  C12N15/8246 ,  C12N15/8261

    Abstract: The present invention discloses isolated polynucleotides encoding WUSCHEL-related homeobox4 proteins from two species of jute plants, namely, the Corchorus olitorius (“C. olitorius”) and Corchorus capsularis (“C. capsularis”), and corresponding polypeptides derived therefrom. The disclosed polynucleotide sequences encode WUSCHEL-related homeobox4 polypeptides (WOX4), which possess catalytic activities in enhancing fiber production in jute. The present invention also relates to the plants having a modulated expression of a nucleic acid encoding a WOX4 polypeptide, which have enhanced fiber yield relative to corresponding wild type plants or other control plants. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the protein are also provided. Also disclosed are methods for producing the proteins and methods for modifying the proteins in order to improve their desirable characteristics. The proteins of the invention can be used in a variety of ways, including inducing, initiating, improving, or enhancing plant growth, plant height, fiber and seed yield.

    Abstract translation: 本发明公开了编码来自两种黄麻植物即柯氏杆菌（“C.olitorius”）和Corchorus capsularis（“C. capsularis”）的两种种类的WUSCHEL相关同源异构体4蛋白质的分离的多核苷酸，以及从其衍生的相应的多肽。 所公开的多核苷酸序列编码WUSCHEL相关的同源异构体4多肽（WOX4），其具有促进黄麻中纤维生产的催化活性。 本发明还涉及具有编码WOX4多肽的核酸调节表达的植物，其相对于相应的野生型植物或其它对照植物具有增强的纤维产量。 还提供了包含和/或由该蛋白质的核苷酸序列组成的载体，表达构建体和宿主细胞。 还公开了用于生产蛋白质的方法和用于修饰蛋白质以改善其期望特征的方法。 本发明的蛋白质可以以多种方式使用，包括诱导，引发，改善或增强植物生长，植物高度，纤维和种子产量。

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