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1.发明申请公开(公告)号：US20150291937A1
公开(公告)日：2015-10-15
申请号：US14421739
申请日：2013-08-15
CPC分类号： C12N9/0061 , C12N9/0065
摘要： The present invention relates to the field of plant breeding and disease resistance in respect of lignin degradation of plant by Macrophomina phaseolina (“M. phaseolina”). The present invention discloses isolated polynucleotide encoding of the lignin degrading enzymes, produced by Macrophomina phaseolina, comprising and consisting of nucleotide sequence set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 65, 68, 71, 74, 77, 80, 83, 86, 89, 92, 95, 98, 101, 104, 107, 110, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 76, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106 and 109 or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66, 69, 72, 75, 78, 81, 84, 87, 90, 93, 96, 99, 102, 105, 108 and 111; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, having enhanced production of lignin degrading enzyme. The invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomerase to fermentable sugar in saccharification process of biomass.
摘要翻译： 本发明涉及通过Macrophomina phaseolina（“M. phaseolina”）对植物的木质素降解的植物育种和抗病性领域。 本发明公开了编码由Macrophomina phaseolina生产的木质素降解酶的分离的多核苷酸，其包含SEQ ID NO： 编号2，5，8，11，14，17，20，23，26，29，32，35，38，41，44，47，50，53，56，59，62，65，68，71 ，74，77，80，83，86，89，92，95，98，101，104，107，110，1,4，7，10，13，16，19，22，25，28，31，34 ，37，40，43，46，49，52，55，58，61，64，67，70，73，76，79，82，85，88，91，94，97，100，103，106和109 或这些序列的补体。 本发明还涉及由SEQ ID No.3,6,9,12,15,18,21,24,27,30,33,36,39,42,45， 48，51，54，57，60，63，66，69，72，75，78，81，84，87，90，93，96，99，102，105，108及111; 包含该多核苷酸的重组基因构建体; 包含重组基因构建体的转化体和转基因真菌，具有增强木质素降解酶的产生。 本发明提供了具有寡聚酶活性的多肽，例如在生物质糖化过程中将顽固性可溶性寡聚酶转化为可发酵糖的酶。
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2.发明申请CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 有权纤维素和/或致细胞毒素降解大蒜素的酶及其用途公开(公告)号：US20150291945A1
公开(公告)日：2015-10-15
申请号：US14421774
申请日：2013-08-15
CPC分类号： C12N9/2402 , C12N9/2408 , C12N9/2428 , C12N9/2437 , C12N9/244 , C12N9/2445 , C12N9/2471 , C12N9/2482 , C12N9/2494 , C12N9/88 , C12Y302/01037 , C12Y302/01051 , C12Y302/01055 , C12Y302/01059 , C12Y302/01075 , C12Y302/01089 , C12Y302/0113 , C12Y302/01177
摘要： The present invention provides nucleotide sequences of Macrophomina phaseolina (“M. phaseolina”) that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a β-glucosidase, a a-glucosidase, a xylanase, a mannanse, a β-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a-fucosidases, a β-galactanase, an unsaturated β-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.
摘要翻译： 本发明提供了编码具有纤维素分解活性的蛋白质/酶的Macrophomina phaseolina（“M. phaseolina”）的核苷酸序列，包括纤维素酶活性，内切葡聚糖酶，纤维二糖水解酶，葡糖苷酶，α-葡糖苷酶，木聚糖酶， 甘露聚糖，α-木糖苷酶，α-木糖苷酶，半乳糖苷酶，阿拉伯呋喃糖苷酶，α-岩藻糖苷酶，β-半乳糖苷酶，不饱和的 - 葡萄糖醛酸水解酶和/或寡聚酶活性。 也提供了包含和/或由该酶基因的核苷酸序列组成的载体，表达构建体和宿主细胞。 本发明还提供了用于生产酶的方法和用于改变酶的方法，以便改善其期望的特性。 本发明的酶可以多种但不限于制药，农业，食品和饲料加工，生物燃料，能源效率和工业环境。 这些酶也可用于将木质纤维素生物质完全水解成单糖，然后将其发酵成液体燃料和化学原料。
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公开(公告)号：US09683221B2
公开(公告)日：2017-06-20
申请号：US14421739
申请日：2013-08-15
CPC分类号： C12N9/0061 , C12N9/0065
摘要： The present invention relates to the field of plant breeding and disease resistance in respect of lignin degradation by Macrophomina phaseolina (“M. phaseolina”). Disclosed are isolated polynucleotides encoding the lignin degrading enzymes produced by Macrophomina phaseolina, or the complement of such sequences. Also disclosed are isolated polypeptides encoded by various polynucleotide sequences; a recombinant gene construct comprising a polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, having enhanced production of lignin degrading enzyme. Further disclosed are polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomerase to fermentable sugar in saccharification process of biomass.
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4.发明申请公开(公告)号：US20150259667A1
公开(公告)日：2015-09-17
申请号：US14421729
申请日：2013-08-15
CPC分类号： C12N9/88 , C12N9/18 , C12N9/2405 , C12Y301/01011 , C12Y402/02002 , C12Y402/02023
摘要： The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina (“M. phaseolina”), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.
摘要翻译： 本发明公开了分离的多核苷酸编码酶，该多核苷酸来自真菌巨噬细胞相容素（“M. phaseolina”），其负责降解果胶，并且其包含和/或由SEQ ID NO：1所示的核苷酸序列组成。 编号2，5，8，11，14，17，20，23，26，29，32，35，38，41，44，47，50，53，56，59，62，1,4,7 ，10,13,16,19,22,25,28,31,34,37,40,43,46,49,52,55,58和61，或这些序列的补体。 本发明还涉及由SEQ ID No.3,6,9,12,15,18,21,24,27,30,33,36,39,42,45， 48，51，54，57，60及63条; 包含该多核苷酸的重组基因构建体; 转化体和转基因真菌，其包含具有或具有增强的果胶降解酶生产的重组基因构建体。 本发明的多肽可以用于制造果汁，纺织品，纸浆和纸，咖啡，茶和油提取和果胶废水处理。
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公开(公告)号：US09765322B2
公开(公告)日：2017-09-19
申请号：US14421729
申请日：2013-08-15
CPC分类号： C12N9/88 , C12N9/18 , C12N9/2405 , C12Y301/01011 , C12Y402/02002 , C12Y402/02023
摘要： The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina (“M. phaseolina”), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.
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6.发明授权公开(公告)号：US09765315B2
公开(公告)日：2017-09-19
申请号：US14421774
申请日：2013-08-15
CPC分类号： C12N9/2402 , C12N9/2408 , C12N9/2428 , C12N9/2437 , C12N9/244 , C12N9/2445 , C12N9/2471 , C12N9/2482 , C12N9/2494 , C12N9/88 , C12Y302/01037 , C12Y302/01051 , C12Y302/01055 , C12Y302/01059 , C12Y302/01075 , C12Y302/01089 , C12Y302/0113 , C12Y302/01177
摘要： The present invention provides nucleotide sequences of Macrophomina phaseolina (“M. phaseolina”) that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a β-glucosidase, a a-glucosidase, a xylanase, a mannanse, a β-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a-fucosidases, a β-galactanase, an unsaturated β-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.
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