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    Aza-benzazolium containing cyanine dyes
    3.
    发明授权
    Aza-benzazolium containing cyanine dyes 有权
    含有氰基染料的氮杂 -

    公开(公告)号：US08883415B2

    公开(公告)日：2014-11-11

    申请号：US13784413

    申请日：2013-03-04

    Applicant: Life Technologies Corporation

    Inventor： Richard Haugland ,  Stephen Yue

    IPC: C12Q1/68 ,  C12Q1/02 ,  C12Q1/04 ,  C12P19/34

    CPC classification number: C09B23/02 ,  C12Q1/6816 ,  C12Q1/6876 ,  G01N1/30 ,  G01N27/44726 ,  G01N33/52 ,  G01N33/5308 ,  G01N33/531 ,  G01N33/582 ,  G01N33/68 ,  G01N2001/302 ,  Y10S436/80 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/107

    Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.

    Abstract translation: 描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。 当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。 描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。

    Site-specific labeling of affinity tags in fusion proteins
    4.
    发明授权
    Site-specific labeling of affinity tags in fusion proteins 有权
    融合蛋白中亲和标签的位点特异性标记

    公开(公告)号：US09164099B2

    公开(公告)日：2015-10-20

    申请号：US13890137

    申请日：2013-05-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Kyle Gee ,  Aleksey Rukavishnikov ,  Courtenay Hart Kerndt ,  Richard Haugland ,  Wai-Yee Leung ,  Wayne Patton ,  Zhenjun Diwu

    IPC: G01N33/533 ,  C07D239/00 ,  C07F5/02 ,  G01N33/58 ,  A61K49/00 ,  C07D311/12 ,  C07D311/18

    CPC classification number: G01N33/52 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07F5/022 ,  G01N33/533 ,  G01N33/582

    Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

    Abstract translation: 本发明提供了通过能够选择性地结合亲和标签来促进融合蛋白的检测和标记的方法和荧光化合物。 荧光化合物具有通式A（B）n，其中A是荧光团，B是作为带电化学部分的结合结构域，其蛋白质或片段，n是1-6的整数，条件是 蛋白质或其片段不是抗体或从抗体产生。 本发明提供用于检测和标记含有多组氨酸亲和标签的融合蛋白的特异性荧光化合物和方法。 这些化合物具有通式A（L）m（B）n，其中A是荧光团，L是连接体，B是乙酸结合域，m是1至4的整数，n是1至 乙酸基团与亲和标签的带正电荷的组氨酸残基直接相互作用，以有效地标记和检测当存在于酸性或中性环境中时含有这种亲和标签的融合蛋白。

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