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公开(公告)号：US10093969B2
公开(公告)日：2018-10-09
申请号：US15695244
申请日：2017-09-05
Applicant: Life Technologies Corporation
Inventor： Stephen Hendricks , David King , Lei Xi , Marian Peris
IPC: C12Q1/68 , C12Q1/6853 , C12P19/34 , C12Q1/6874 , C12Q1/6869 , C12Q1/6834 , C12Q1/6844 , C12Q1/46
Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.
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公开(公告)号：US09315861B2
公开(公告)日：2016-04-19
申请号：US14360892
申请日：2012-11-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Stephen Hendricks , David King , Lei Xi , Marian Peris
CPC classification number: C12Q1/46 , C12P19/34 , C12Q1/6834 , C12Q1/6846 , C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2531/119 , C12Q2565/537
Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.
Abstract translation: 在一些实施方案中，用于连接核酸末端的方法包括：在存在可产生可连接的末端5'磷酸基团的至少一种试剂的存在下进行核酸连接反应，通过从核酸的末端5'磷酸脱除腺苷酸基 。 在一些实施方案中，阿伐他汀酶可以催化从核酸的末端5'磷酸脱除腺苷酸基团。 在一些实施方案中，用于连接核酸末端的方法包括：在适于连接核酸末端的条件下，在阿伐他汀酶存在下进行核酸连接反应。
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公开(公告)号：US20140378315A1
公开(公告)日：2014-12-25
申请号：US13980280
申请日：2012-01-17
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Stephen Hendricks
CPC classification number: C12Q1/6874 , A61J1/062 , A61J1/14 , A61J2200/76 , A61J2205/30 , A61M5/008 , A61M5/5086 , B65B3/003 , B65B3/006 , B65B31/046 , B65B55/08 , B65B55/10 , B65D15/02 , C12N9/93 , C12Q1/6876 , C12Q2600/16 , C12Y605/01001
Abstract: Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.
Abstract translation: 本文提供了用于短多核苷酸连接的方法，测定，组合物和试剂盒。 短多核苷酸长度上任选不超过7个核苷酸，长度可短至3或4个核苷酸。 连接可任选由CV连接酶进行。
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4.发明申请公开(公告)号：US20170044506A1
公开(公告)日：2017-02-16
申请号：US15243808
申请日：2016-08-22
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Lei (Larry) XI , Roland Nagel , Stephen Hendricks , Jennifer Berkman , Marian Peris , Yulei Wang
CPC classification number: C12N9/1276 , C12N15/1096 , C12Q2521/107 , C12Y207/07049
Abstract: The present invention provides compositions and methods for a reverse transcription reaction using a reversibly inactivated reverse transcriptase enzyme. The reversibly inactivated reverse transcriptase enzyme results from a chemical modification which inactivates the reverse transcriptase enzyme. The activity of the reverse transcriptase enzyme is recovered by an incubation of the reaction mixture at elevated temperature prior to, or as part of the reverse transcription reaction. The reverse transcriptase enzyme of the present invention provides for a significant reduction in non-specific reverse transcription from template nucleic acid molecules because the formulation of the reaction mixture does not support the formation of reverse transcription products prior to activation of the reverse transcriptase.
Abstract translation: 本发明提供使用可逆失活的逆转录酶进行逆转录反应的组合物和方法。 可逆灭活的逆转录酶由化学修饰产生，其使逆转录酶失活。 通过在反转录反应之前或作为逆转录反应的一部分的升高温度下温育反应物来回收逆转录酶的活性。 本发明的逆转录酶提供了来自模板核酸分子的非特异性逆转录的显着降低，因为反应混合物的配方不支持在逆转录酶激活之前形成逆转录产物。
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公开(公告)号：US20160153035A1
公开(公告)日：2016-06-02
申请号：US14955386
申请日：2015-12-01
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： SHIAW-MIN CHEN , Elana Swartzman , David Ruff , Mark Shannon , Julia Lu , Stephen Hendricks
IPC: C12Q1/68
Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.
Abstract translation: 本申请涉及用于连接与靶核酸互补的寡核苷酸的方法，并扩增连接的寡核苷酸，其中连接和扩增发生在相同的反应混合物中。
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Patent Agency Ranking
公开(公告)号：US11661629B2
公开(公告)日：2023-05-30
申请号：US16666188
申请日：2019-10-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Stephen Hendricks , David J. King
IPC: C12Q1/6874 , A61J1/06 , A61J1/14 , B65B3/00 , B65B31/04 , B65D8/00 , C12N9/00 , C12Q1/6876 , A61M5/00 , A61M5/50 , B65B55/08 , B65B55/10
CPC classification number: C12Q1/6874 , A61J1/062 , A61J1/14 , B65B3/003 , B65B3/006 , B65B31/046 , B65D15/02 , C12N9/93 , C12Q1/6876 , A61J2200/76 , A61J2205/30 , A61M5/008 , A61M5/5086 , B65B55/08 , B65B55/10 , C12Q2600/16 , C12Y605/01001
Abstract: Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.
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公开(公告)号：US10336991B2
公开(公告)日：2019-07-02
申请号：US15422224
申请日：2017-02-01
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Marian Peris , Michael Phelan , Barnett Rosenblum , Stephen Hendricks
IPC: C12N9/12 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Q1/6804 , C12Q1/6818 , C12Q1/68
Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
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公开(公告)号：US09765388B2
公开(公告)日：2017-09-19
申请号：US15071086
申请日：2016-03-15
Applicant: Life Technologies Corporation
Inventor： Stephen Hendricks , David King , Lei Xi , Marian Peris
CPC classification number: C12Q1/46 , C12P19/34 , C12Q1/6834 , C12Q1/6846 , C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2531/119 , C12Q2565/537
Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.
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公开(公告)号：US09593315B2
公开(公告)日：2017-03-14
申请号：US15200670
申请日：2016-07-01
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Marian Peris , Michael Phelan , Barnett Rosenblum , Stephen Hendricks
CPC classification number: C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
Abstract translation: 本文提供了衍生自野生型RB69 DNA聚合酶或与野生型RB69 DNA聚合酶相关的突变型DNA依赖性聚合酶。 这些突变型聚合酶能够选择性地结合标记的核苷酸。 这些突变型聚合酶还能够掺入多种天然存在和修饰的核苷酸，包括例如终止子核苷酸。
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公开(公告)号：US09476093B2
公开(公告)日：2016-10-25
申请号：US14593858
申请日：2015-01-09
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Lei Xi , Paul Kenney , Zhaochun Ma , Dennis Prosen , Stephen Hendricks , Roland Nagel
CPC classification number: C12Q1/686 , C07H21/00 , C07H21/02 , C12N9/1252 , C12N15/10 , C12Q1/6869 , C12Y207/07007
Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.
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