-
公开(公告)号:US09447463B2
公开(公告)日:2016-09-20
申请号:US14101226
申请日:2013-12-09
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Kai Lao , Neil Straus , Nanlan Xu
IPC: C12Q1/68
CPC classification number: C12Q1/6853 , C12Q1/6844 , C12Q1/6848 , C12Q2600/158 , C12Q2525/301 , C12Q2525/207 , C12Q2525/161
Abstract: The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.
Abstract translation: 本公开涉及用于各种测序和/或鉴定技术的靶核酸序列的扩增。 如本文所述,这些引物的使用允许减少非特异性杂交事件(例如引物二聚化)的扩增,同时允许靶核酸序列的扩增。
-
公开(公告)号:US09528147B2
公开(公告)日:2016-12-27
申请号:US14723304
申请日:2015-05-27
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Kai Lao , Neil Straus
CPC classification number: C12Q1/6853 , C12Q1/6848 , C12Q1/6876 , C12Q2549/119 , C12Q2525/301 , C12Q2525/155
Abstract: The present disclosure relates to the amplification of target nucleic acid sequences. This can be accomplished via the use of various primers. The use of these primers, as described herein, results in nucleic acid structures that can reduce the amplification of nonspecific hybridization events (such as primer dimerization) while allowing the amplification of the target nucleic acid sequences.
Abstract translation: 本公开涉及靶核酸序列的扩增。 这可以通过使用各种引物来实现。 如本文所述,使用这些引物导致可以减少非特异性杂交事件(例如引物二聚化)的扩增同时允许扩增靶核酸序列的核酸结构。
-
公开(公告)号:US10913976B2
公开(公告)日:2021-02-09
申请号:US16351194
申请日:2019-03-12
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin Li , Kai Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
IPC: C12Q1/68 , C12Q1/6853 , C12Q1/6844 , C12Q1/6806 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q1/6834 , C12Q1/46
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
-
公开(公告)号:US20190264272A1
公开(公告)日:2019-08-29
申请号:US16351194
申请日:2019-03-12
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin LI , Kai Lao , Jennifer O'NEIL , Jennifer KUNKEL , Kellie HALEY , Rachel KASINSKAS , Zhaochun MA , Pius BRZOSKA
IPC: C12Q1/6853 , C12Q1/6834 , C12Q1/6874 , C12Q1/46 , C12Q1/6844 , C12Q1/6869 , C12P19/34
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
-
-
-