公开(公告)号：US09447463B2

公开(公告)日：2016-09-20

申请号：US14101226

申请日：2013-12-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kai Lao ,  Neil Straus ,  Nanlan Xu

IPC: C12Q1/68

CPC classification number: C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q2600/158 ,  C12Q2525/301 ,  C12Q2525/207 ,  C12Q2525/161

Abstract: The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.

Abstract translation: 本公开涉及用于各种测序和/或鉴定技术的靶核酸序列的扩增。 如本文所述，这些引物的使用允许减少非特异性杂交事件（例如引物二聚化）的扩增，同时允许靶核酸序列的扩增。

公开(公告)号：US20140206000A1

公开(公告)日：2014-07-24

申请号：US14101226

申请日：2013-12-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kai LAO ,  Neil Straus ,  Nanlan Xu

IPC: C12Q1/68

CPC classification number: C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q2600/158 ,  C12Q2525/301 ,  C12Q2525/207 ,  C12Q2525/161

Abstract: The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.

Abstract translation: 本公开涉及用于各种测序和/或鉴定技术的靶核酸序列的扩增。 如本文所述，这些引物的使用允许减少非特异性杂交事件（例如引物二聚化）的扩增，同时允许靶核酸序列的扩增。