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1.发明申请METHOD OF DETERMINING INITIAL CONCENTRATION OF NUCLEIC ACID IN SAMPLE USING REAL-TIME AMPLIFICATION DATA 有权使用实时放大数据确定样品中核酸初始浓度的方法公开(公告)号：US20090150085A1
公开(公告)日：2009-06-11
申请号：US12259548
申请日：2008-10-28
Applicant: Kak Namkoong , Young-sun LEE , Chin-sung PARK , Joo-won RHEE
Inventor： Kak Namkoong , Young-sun LEE , Chin-sung PARK , Joo-won RHEE
CPC classification number: C12Q1/6851 , C12Q2537/165
Abstract: Disclosed is a method of determining an initial concentration of a target nucleic acid within a sample using real-time nucleic acid amplification data. Amplification efficiencies of the target nucleic acid with respect to amplification time are obtained from signals of amplified products, and an amplification efficiency function with respect to amplification time is formulated employing the amplification efficiencies.
Abstract translation: 公开了使用实时核酸扩增数据确定样品中靶核酸的初始浓度的方法。 从放大产物的信号获得靶核酸相对于扩增时间的扩增效率，并且使用扩增效率来制定关于扩增时间的扩增效率函数。
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2.发明授权公开(公告)号：US08278051B2
公开(公告)日：2012-10-02
申请号：US13185003
申请日：2011-07-18
Applicant: Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
Inventor： Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
CPC classification number: C12Q1/6844 , C12Q1/6855 , C12Q2525/301 , C12Q2531/125 , C12Q2537/101 , C12Q2521/301 , C12Q2521/501
Abstract: Provided is a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase, (d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
Abstract translation: 提供了使用滚动循环扩增（RCA）扩增核酸的方法，包括使用RCA形成单链环状DNA模板来扩增核酸，反应溶液包括：（a）两个发夹寡核苷酸（ b）靶核酸，（c）DNA连接酶，（d）内切核酸酶，（e）DNA聚合酶和（f）引物。
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3.发明授权Method of determining initial concentration of nucleic acid in sample using real-time amplification data 有权使用实时放大数据确定样品中核酸初始浓度的方法公开(公告)号：US08185324B2
公开(公告)日：2012-05-22
申请号：US12259548
申请日：2008-10-28
Applicant: Kak Namkoong , Young-sun Lee , Chin-sung Park , Joo-won Rhee
Inventor： Kak Namkoong , Young-sun Lee , Chin-sung Park , Joo-won Rhee
IPC: G01N33/48
CPC classification number: C12Q1/6851 , C12Q2537/165
Abstract: Disclosed is a method of determining an initial concentration of a target nucleic acid within a sample using real-time nucleic acid amplification data. Amplification efficiencies of the target nucleic acid with respect to amplification time are obtained from signals of amplified products, and an amplification efficiency function with respect to amplification time is formulated employing the amplification efficiencies.
Abstract translation: 公开了使用实时核酸扩增数据确定样品中靶核酸的初始浓度的方法。 从放大产物的信号获得靶核酸相对于扩增时间的扩增效率，并且使用扩增效率来制定关于扩增时间的扩增效率函数。
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4.发明授权公开(公告)号：US08003322B2
公开(公告)日：2011-08-23
申请号：US12247433
申请日：2008-10-08
Applicant: Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
Inventor： Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
CPC classification number: C12Q1/6844 , C12Q1/6855 , C12Q2525/301 , C12Q2531/125 , C12Q2537/101 , C12Q2521/301 , C12Q2521/501
Abstract: Provided is kit for and a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase,(d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
Abstract translation: 提供了使用滚动循环扩增（RCA）扩增核酸的试剂盒和使用RCA形成单链环状DNA模板的核酸的方法，所述反应溶液包括：（a）两个发夹 寡核苷酸，（b）靶核酸，（c）DNA连接酶，（d）内切核酸酶，（e）DNA聚合酶和（f）引物。
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公开(公告)号：US08136550B2
公开(公告)日：2012-03-20
申请号：US12023197
申请日：2008-01-31
Applicant: Kak Namkoong , Chin-sung Park , Jin-tae Kim , Joo-won Rhee
Inventor： Kak Namkoong , Chin-sung Park , Jin-tae Kim , Joo-won Rhee
IPC: E03B1/00
CPC classification number: F16K99/0001 , Y10T137/87434 , Y10T137/877
Abstract: An elastic valve and a microfluidic device including the same are provided. The elastic valve includes a channel dented by external pressure so as to be elastically restituted; and a plurality of channel closing protrusions, which comprise elastomer as a material, which are protruded from at least one inner side surface of the channel toward an opposite inner side surface, and which are separated from each other so as not to interrupt a flow of fluids, wherein, when external pressure is applied in order to dent the channel, the plurality of channel closing protrusions are deformed so as to be elastically restituted, thereby closing the channel.
Abstract translation: 提供弹性阀和包括该弹性阀的微流体装置。 弹性阀包括通过外部压力凹陷以便被弹性恢复的通道; 以及多个通道闭合突起，其包括弹性体作为材料，其从所述通道的至少一个内侧表面突出到相对的内侧表面，并且彼此分离，以便不会中断流动 流体，其中当施加外部压力以凹陷通道时，多个通道关闭突起变形以便被弹性地恢复，从而关闭通道。
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Patent Agency Ranking
公开(公告)号：US07838300B2
公开(公告)日：2010-11-23
申请号：US11689640
申请日：2007-03-22
Applicant: Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
Inventor： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
CPC classification number: B81B3/0081 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00873 , B01L3/5027 , B01L7/5255 , B01L2200/14 , B01L2200/147 , B01L2300/1805 , B01L2300/1827 , B81B2201/051 , F28F21/02 , F28F2013/006
Abstract: A method of reducing a temperature difference between a high-temperature and a low-temperature substrate includes interposing a heat transfer facilitating layer which has a higher thermal conductivity than air and can hold particles between the substrates, and maintaining close contact between the high-temperature substrate, the heat transfer facilitating layer, and the low-temperature substrate, wherein formation of an air layer can be at least substantially prevented between the high-temperature substrate and the heat transfer facilitating layer, and between the low-temperature substrate and the heat transfer facilitating layer.
Abstract translation: 降低高温和低温基板之间的温度差的方法包括：介于热传导率高于空气的传热促进层，并且可以在基板之间保持颗粒，并且保持高温 基板，传热促进层和低温基板，其中至少可以在高温基板和传热促进层之间以及在低温基板和热量之间基本上防止空气层的形成 转移促进层。
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7.发明申请MICRO-FLUID REACTION VESSEL, METHOD FOR MANUFACTURING THE SAME, AND MICRO-FLUID REACTION METHOD USING THE VESSEL 有权微流体反应容器，其制造方法和使用容器的微流体反应方法公开(公告)号：US20070254379A1
公开(公告)日：2007-11-01
申请号：US11625877
申请日：2007-01-23
Applicant: Su-hyeon KIM , Jin-tae KIM , Kak Namkoong , In-ho LEE , Chin-sung PARK , Young-sun LEE
Inventor： Su-hyeon KIM , Jin-tae KIM , Kak Namkoong , In-ho LEE , Chin-sung PARK , Young-sun LEE
CPC classification number: B01L3/502738 , B01L7/52 , B01L2200/0689 , B01L2300/0816 , B01L2300/0825 , B01L2300/0887 , B01L2300/123 , B01L2400/0655 , B29C33/3857 , B29C33/60 , Y10T137/218 , Y10T137/2202 , Y10T137/2224
Abstract: A micro-fluid reaction vessel includes an upper plate formed of an elastomer, a lower plate adhered to the upper plate, a micro-chamber and a micro-channel formed on an inner surface of the upper plate facing the lower plate and an inlet hole and an outlet hole formed in the upper plate and through which a fluid flows into or out of, respectively. The micro-channel is constructed to be closed by pressure applied to the upper plate and elastically restored when the pressure is not applied. A micro fluid reaction method uses the micro fluid reaction vessel and a method of manufacturing forms the microfluid reaction vessel.
Abstract translation: 微流体反应容器包括由弹性体形成的上板，粘附到上板的下板，形成在面向下板的上板的内表面上的微室和微通道，以及入口孔 以及形成在上板中并且流体分别流入或流出的出口孔。 微通道被构造为通过施加到上板的压力而关闭，并且当不施加压力时弹性恢复。 微流体反应方法使用微流体反应容器和制造方法形成微流体反应容器。
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公开(公告)号：US20090170189A1
公开(公告)日：2009-07-02
申请号：US12184317
申请日：2008-08-01
Applicant: Chin-sung PARK , Kak Namkoong , Young-sun Lee
Inventor： Chin-sung PARK , Kak Namkoong , Young-sun Lee
CPC classification number: C12M23/16 , B01L2200/12 , B01L2300/0816 , B01L2300/1827 , B29C65/02 , B29C65/08 , B29C66/1122 , B29C66/30223 , B29C66/47 , B29C66/53461 , B29C66/61 , B29C66/71 , B29C66/7352 , B29C66/73921 , B29C66/8322 , B29K2023/06 , B29K2023/12 , B29K2033/12 , B29K2067/00 , B29K2069/00 , B29L2031/756 , Y10T156/10 , B29K2067/003 , B29K2023/38
Abstract: Provided are a microfluidic device that performs a biochemical reaction using a small amount of a biochemical fluid and detects the result thereof, and a method of fabricating the same. The microfluidic device includes: a substrate which comprises a chamber that is formed as a concave groove and accommodates a fluid in the bottom surface of the substrate, and is formed of polymer; and a film welded on the bottom surface of the substrate to seal the chamber so that the chamber is not open at the bottom surface of the substrate, and formed of polymer. The method of fabricating a microfluidic device includes: preparing a substrate which comprises a chamber that is formed as a concave groove and accommodates a fluid in the bottom surface of the substrate, and is formed of polymer; and welding a film on a bottom surface of the substrate to seal the chamber so that the chamber is not opened at the bottom surface of the substrate, the film being formed of polymer.
Abstract translation: 提供了使用少量生物化学流体进行生化反应并检测其结果的微流体装置及其制造方法。 微流体装置包括：基板，其包括形成为凹槽并容纳基板的底表面中的流体的腔室，并且由聚合物形成; 以及焊接在基板的底表面上的膜以密封室，使得室不在基板的底表面处开口，并且由聚合物形成。 制造微流体装置的方法包括：制备基板，其包括形成为凹槽的腔室，并且在基底的底表面容纳流体，并且由聚合物形成; 并且在所述基板的底表面上焊接膜以密封所述室，使得所述室在所述基板的底表面处不被打开，所述膜由聚合物形成。
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9.发明授权Method of reducing temperature difference between a pair of substrates and fluid reaction device using the same 失效降低一对基板与使用其的液体反应装置之间的温度差的方法公开(公告)号：US08771610B2
公开(公告)日：2014-07-08
申请号：US12904557
申请日：2010-10-14
Applicant: Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
Inventor： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
IPC: B01L3/00
CPC classification number: B81B3/0081 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00873 , B01L3/5027 , B01L7/5255 , B01L2200/14 , B01L2200/147 , B01L2300/1805 , B01L2300/1827 , B81B2201/051 , F28F21/02 , F28F2013/006
Abstract: A fluid reaction device includes a microfluidic reaction chip which accommodates a fluid, a heater, and a heat transfer facilitating layer which is interposed between the microfluidic reaction chip and the heater, the heat transfer facilitating layer has a higher thermal conductivity than air and can hold particles, wherein formation of an air layer can be prevented.
Abstract translation: 流体反应装置包括容纳流体的微流体反应芯片，加热器和介于微流体反应芯片和加热器之间的传热促进层，传热促进层具有比空气更高的导热性并且可以保持 颗粒，其中可以防止空气层的形成。
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10.发明申请METHOD OF REDUCING TEMPERATURE DIFFERENCE BETWEEN A PAIR OF SUBSTRATES AND FLUID REACTION DEVICE USING THE SAME 有权降低基板对流与使用其的流体反应装置之间的温度差异的方法公开(公告)号：US20080053647A1
公开(公告)日：2008-03-06
申请号：US11689640
申请日：2007-03-22
Applicant: Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
Inventor： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
IPC: F28F7/00
CPC classification number: B81B3/0081 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00873 , B01L3/5027 , B01L7/5255 , B01L2200/14 , B01L2200/147 , B01L2300/1805 , B01L2300/1827 , B81B2201/051 , F28F21/02 , F28F2013/006
Abstract: A method of reducing a temperature difference between a high-temperature and a low-temperature substrate includes interposing a heat transfer facilitating layer which has a higher thermal conductivity than air and can hold particles between the substrates, and maintaining close contact between the high-temperature substrate, the heat transfer facilitating layer, and the low-temperature substrate, wherein formation of an air layer can be at least substantially prevented between the high-temperature substrate and the heat transfer facilitating layer, and between the low-temperature substrate and the heat transfer facilitating layer. A fluid reaction device includes a microfluidic reaction chip which accommodates a fluid, a heater, and a heat transfer facilitating layer which is interposed between the microfluidic reaction chip and the heater, the heat transfer facilitating layer has a higher thermal conductivity than air and can hold particles, wherein formation of an air layer can be prevented.
Abstract translation: 降低高温和低温基板之间的温度差的方法包括：介于热传导率高于空气的传热促进层，并且可以在基板之间保持颗粒，并且保持高温 基板，传热促进层和低温基板，其中至少可以在高温基板和传热促进层之间以及在低温基板和热量之间基本上防止空气层的形成 转移促进层。 流体反应装置包括容纳流体的微流体反应芯片，加热器和介于微流体反应芯片和加热器之间的传热促进层，传热促进层具有比空气更高的导热性并且可以保持 颗粒，其中可以防止空气层的形成。
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