Invention Grant
- Patent Title: Method of amplifying a target nucleic acid by rolling circle amplification
- Patent Title (中): 通过滚圆放大扩增靶核酸的方法
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Application No.: US13185003Application Date: 2011-07-18
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Publication No.: US08278051B2Publication Date: 2012-10-02
- Inventor: Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
- Applicant: Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
- Applicant Address: KR Suwon-si
- Assignee: Samsung Electronics Co., Ltd.
- Current Assignee: Samsung Electronics Co., Ltd.
- Current Assignee Address: KR Suwon-si
- Agency: Sughrue Mion, PLLC
- Priority: KR10-2007-0102581 20071011
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
Provided is a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase, (d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
Public/Granted literature
- US20110269193A1 METHOD OF AMPLIFYING A TARGET NUCLEIC ACID BY ROLLING CIRCLE AMPLIFICATION Public/Granted day:2011-11-03
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