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    METHODS AND REAGENTS FOR IN VIVO IMAGING OF CANCER CELL LINES
    1.
    发明申请
    METHODS AND REAGENTS FOR IN VIVO IMAGING OF CANCER CELL LINES 审中-公开
    癌细胞系血管成像的方法和试剂

    公开(公告)号：US20090311193A1

    公开(公告)日：2009-12-17

    申请号：US12293982

    申请日：2007-03-23

    申请人： John Mauro ,  Thomas Steinberg ,  Julie Nyhus

    发明人： John Mauro ,  Thomas Steinberg ,  Julie Nyhus

    IPC分类号： A61K49/00 ,  C07K16/18

    CPC分类号： G01N33/57484 ,  A61K49/0021 ,  A61K49/0058 ,  G01N33/532 ,  G01N33/54346

    摘要： Provided are reagents and methods for non-invasive in vivo imaging wherein the reagents comprise targeted carrier molecules conjugated to a NIR reporter molecule. In one aspect the targeted carrier molecule is an antibody, or fragment thereof that has specificity for an antigen in a living body, animal or human. In one embodiment the antibodies are anti-cancer/tumor marker antibodies, organ specific antibodies, tissue specific antibodies, cell type specific antibodies, cell surface specific antibodies, anti-viral antibodies, anti-bacterial antibodies and anti-pathogenic antibodies. The NIR reporter molecules are any fluorescent reporter molecule compatible with in vivo imaging and generally having an excitation wavelength of at least 580 nm.

    摘要翻译： 提供了用于非侵入性体内成像的试剂和方法，其中试剂包含与NIR报告分子缀合的靶载体分子。 一方面，靶向载体分子是对活体，动物或人体中的抗原具有特异性的抗体或其片段。 在一个实施方案中，抗体是抗癌/肿瘤标志物抗体，器官特异性抗体，组织特异性抗体，细胞类型特异性抗体，细胞表面特异性抗体，抗病毒抗体，抗细菌抗体和抗致病性抗体。 NIR报告分子是与体内成像相容的任何荧光报道分子，通常具有至少580nm的激发波长。

    Methods and Reagents for in Vivo Imaging of Cancel Cell Lines
    4.
    发明申请
    Methods and Reagents for in Vivo Imaging of Cancel Cell Lines 审中-公开
    取消细胞系体内成像的方法和试剂

    公开(公告)号：US20120253160A1

    公开(公告)日：2012-10-04

    申请号：US13439279

    申请日：2012-04-04

    申请人： John Mauro ,  Thomas Steinberg ,  Julie Nyhus

    发明人： John Mauro ,  Thomas Steinberg ,  Julie Nyhus

    IPC分类号： A61B5/1455

    CPC分类号： G01N33/57484 ,  A61K49/0021 ,  A61K49/0058 ,  G01N33/532 ,  G01N33/54346

    摘要： Provided is reagents and methods for non-invasive in vivo imaging wherein the reagents comprise targeted carrier molecules conjugated to a NIB reporter molecule. In one aspect the targeted carrier molecule is an antibody, or fragment thereof that has specificity for an antigen in a living body, animal or human. In one embodiment the antibodes are anti-cancer/tumor marker antibodies, organ specific antibodies, tissue specific antibodies, cell type specific antibodies, cell surface specific antibodies, anti-viral antibodies, anti-bacterial antibodies and anti-pathogenic antibodies. The NIP reporter molecules are any fluorescent reporter molecule compatible with in vivo imaging and generally having an excitation wavelength of at least 580 nm.

    摘要翻译： 提供了用于非侵入性体内成像的试剂和方法，其中试剂包含与NIB报道分子缀合的靶向载体分子。 一方面，靶向载体分子是对活体，动物或人体中的抗原具有特异性的抗体或其片段。 在一个实施方案中，抗菌剂是抗癌/肿瘤标志物抗体，器官特异性抗体，组织特异性抗体，细胞型特异性抗体，细胞表面特异性抗体，抗病毒抗体，抗细菌抗体和抗致病性抗体。 NIP报告分子是与体内成像相容的任何荧光报道分子，通常具有至少580nm的激发波长。

    Compositions and methods for detection and isolation of phosphorylated molecules
    8.
    发明申请
    Compositions and methods for detection and isolation of phosphorylated molecules 审中-公开
    用于磷酸化分子检测和分离的组合物和方法

    公开(公告)号：US20100009381A1

    公开(公告)日：2010-01-14

    申请号：US12500854

    申请日：2009-07-10

    申请人： Brian Agnew ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Thomas Steinberg ,  Wayne Patton

    发明人： Brian Agnew ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Thomas Steinberg ,  Wayne Patton

    IPC分类号： G01N33/53

    CPC分类号： G01N33/5008 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/502 ,  G01N33/5091 ,  G01N2333/4709 ,  Y10T436/142222 ,  Y10T436/145555 ,  Y10T436/147777 ,  Y10T436/201666

    摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

    摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

    Compositions and methods for detection and isolation of phosphorylated molecules
    9.
    发明授权
    Compositions and methods for detection and isolation of phosphorylated molecules 有权
    用于磷酸化分子检测和分离的组合物和方法

    公开(公告)号：US07102005B2

    公开(公告)日：2006-09-05

    申请号：US10428192

    申请日：2003-05-02

    申请人： Brian Agnew ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Jixiang Liu ,  Vladimir Martin ,  Wayne Patton ,  Thomas Steinberg

    发明人： Brian Agnew ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Jixiang Liu ,  Vladimir Martin ,  Wayne Patton ,  Thomas Steinberg

    IPC分类号： C07D239/88 ,  C07D277/62 ,  C07D311/82 ,  C07D417/02 ,  C07D495/04

    CPC分类号： G01N33/50 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5091 ,  G01N2333/4709

    摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

    摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

    Reagents for labeling biomolecules having aldehyde or ketone moieties
    10.
    发明申请
    Reagents for labeling biomolecules having aldehyde or ketone moieties 有权
    用于标记具有醛或酮部分的生物分子的试剂

    公开(公告)号：US20050250152A1

    公开(公告)日：2005-11-10

    申请号：US11182122

    申请日：2005-07-15

    申请人： Richard Haugland ,  Thomas Steinberg ,  Wayne Patton ,  Zhenjun Diwu

    发明人： Richard Haugland ,  Thomas Steinberg ,  Wayne Patton ,  Zhenjun Diwu

    IPC分类号： G01N33/532 ,  C07D239/91 ,  C07D239/94 ,  C07D249/08 ,  C07D263/60 ,  C07D265/22 ,  C07D277/66 ,  C07D401/04 ,  G01N33/58 ,  C12Q1/68 ,  C07H21/04 ,  C08B37/00

    CPC分类号： C07D265/22 ,  C07D239/91 ,  Y10T436/143333 ,  Y10T436/200833

    摘要： Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene, or a single covalent bond; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-6alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-21-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, or sulfo; and R21-R24 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The method of treating a sample with the derivativization reagents is described. The reagents are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.

    摘要翻译： 描述了适用于与含有醛或酮官能团的生物分子偶联的新型荧光衍生试剂。 在一个实施方案中，提供具有下式的试剂：其中Q是羰基，硫代羰基或磺酰基，R 5是-L-Z; L是亚芳基或C 1-6个全氟亚烷基，或单个共价键; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R 11 -R 14独立地为H，C 1-6烷基，C 1-6烷氧基，C 全氟烷基，C 1-6烷基氨基，二（C 1 -C 21 - 亚烷基）氨基，氨基，羧基，氰基，卤素 ，羟基，硝基，苯基或磺基; R 21和R 24独立地是H，C 1-6烷基，C 1-6烷氧基， C 1-6烷基全氟烷基，C 1-6烷基氨基，二（C 2-12-12 - 烷基）氨基，氨基，羧基，氰基， 卤素，羟基，硝基，苯基，磺基，或-LZ。 描述了用衍生化试剂处理样品的方法。 这些试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。