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公开(公告)号：US20240287505A1
公开(公告)日：2024-08-29
申请号：US18567697
申请日：2022-06-23
申请人： Illumina, Inc.
发明人： Andrea Manzo , Colin Brown , Steven Norberg , Timothy Harrington
IPC分类号： C12N15/10 , C12Q1/6834
CPC分类号： C12N15/1065 , C12Q1/6834
摘要： Some embodiments relate to methods and compositions for preparing combinatorially indexed beads. Some embodiments include sequential addition of different indexes to polynucleotides attached to beads. In some embodiments, indexes are added by chemical ligation, polymerase extension, ligation of partially double-stranded adaptors, or short splint ligation.
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公开(公告)号：US20210332349A1
公开(公告)日：2021-10-28
申请号：US17305230
申请日：2021-07-01
申请人： Illumina, Inc.
发明人： Steven Norberg , Dmitry Pokholok , Ramesh Ramji , Frank Steemers , Fan Zhang
摘要： Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.
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公开(公告)号：US11999945B2
公开(公告)日：2024-06-04
申请号：US17305230
申请日：2021-07-01
申请人： Illumina, Inc.
CPC分类号： C12N15/1006 , B01L3/502761 , C12N15/1065 , C40B30/00 , C40B60/02 , C40B80/00
摘要： Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.
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公开(公告)号：US11085036B2
公开(公告)日：2021-08-10
申请号：US16663060
申请日：2019-10-24
申请人： Illumina, Inc.
发明人： Steven Norberg , Dmitry Pokholok , Ramesh Ramji , Frank J. Steemers , Fan Zhang
摘要： Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.
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公开(公告)号：US12104281B2
公开(公告)日：2024-10-01
申请号：US17250848
申请日：2019-11-25
申请人： ILLUMINA, INC.
IPC分类号： C40B50/06 , C12Q1/6806 , C12Q1/6834
CPC分类号： C40B50/06 , C12Q1/6806 , C12Q1/6834 , C12Q2563/179
摘要： Embodiments of systems, methods, and compositions provided herein relate to methods of simultaneously analyzing multiple analytes in a single sample using a single assay. Some embodiments relate to simultaneous analysis of DNA and RNA in a single sample, for example, to the simultaneous generation of DNA and RNA libraries.
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公开(公告)号：US20200165650A1
公开(公告)日：2020-05-28
申请号：US16659475
申请日：2019-10-21
申请人： Illumina, Inc.
发明人： Gordon M. Cann , Jeffrey G. Mandell , Alex Aravanis , Steven Norberg , Dmitry K. Pokholok , Frank J. Steemers , Farnaz Absalan , Leila Bazargan
IPC分类号： C12P19/34 , C12Q1/683 , C12Q1/6869 , C12Q1/6816 , C12N15/10
摘要： A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.
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公开(公告)号：US20180355348A1
公开(公告)日：2018-12-13
申请号：US16000505
申请日：2018-06-05
IPC分类号： C12N15/10
CPC分类号： C12N15/1065 , C12N15/1093 , C12Q1/6806 , C12Q1/6874 , C12Q2523/125 , C40B50/18 , C12Q2523/101 , C12Q2525/191 , C12Q2535/122 , C12Q2537/143 , C12Q2537/159 , C12Q2563/159 , C12Q2563/179
摘要： Provided herein are methods for preparing sequencing libraries for determining the methylation status of nucleic acids from a plurality of single cells. The present methods combine split-and-pool combinatorial indexing and bisulfite treatment techniques to characterize the methylation profiles of large numbers of single cells quickly, accurately and inexpensively.
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公开(公告)号：US11359226B2
公开(公告)日：2022-06-14
申请号：US16384741
申请日：2019-04-15
申请人： ILLUMINA, INC.
发明人： Frank J. Steemers , Ramesh Ramji , Steven Norberg , Lena Christiansen , Dmitry K. Pokholok , Fan Zhang
摘要： Embodiments of systems, methods, and compositions provided herein relate to hollow beads encapsulating single cells. Some embodiments include performing multiple co-assays on a single cell encapsulated within a hollow bead, including nucleic acid sequencing, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.
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公开(公告)号：US20190352591A1
公开(公告)日：2019-11-21
申请号：US16384741
申请日：2019-04-15
申请人： ILLUMINA, INC.
发明人： Frank J. Steemers , Ramesh Ramji , Steven Norberg , Lena Christiansen , Dmitry K. Pokholok , Fan Zhang
摘要： Embodiments of systems, methods, and compositions provided herein relate to hollow beads encapsulating single cells. Some embodiments include performing multiple co-assays on a single cell encapsulated within a hollow bead, including nucleic acid sequencing, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.
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公开(公告)号：US20160017396A1
公开(公告)日：2016-01-21
申请号：US14804068
申请日：2015-07-20
申请人： ILLUMINA, INC.
发明人： Gordon M. Cann , Jeffrey G. Mandell , Alex Aravanis , Steven Norberg , Dmitry K. Pokholok , Frank J. Steemers , Farnaz Absalan , Leila Bazargan
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12N15/102 , C12Q1/6816 , C12Q1/683 , C12Q1/6869 , C12Q2521/301 , C12Q2522/101 , C12Q2563/131
摘要： A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.
摘要翻译： 一种富含靶核酸的方法，包括提供具有crRNA或其衍生物的内切核酸酶系统和Cas蛋白质或其变体。 所述crRNA或其衍生物含有与靶核酸区域基本上互补的靶特异性核苷酸区域; 使靶核酸与内切核酸酶系统接触以形成复合物; 并分离复合物，从而富集靶核酸。
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