-
公开(公告)号：US11613773B2
公开(公告)日：2023-03-28
申请号：US17834474
申请日：2022-06-07
发明人： Jonas Frisen , Patrik Stahl , Joakim Lundeberg , Gordon M. Cann , Leila Bazargan , Alex Aravanis
IPC分类号： C12Q1/6834 , C12Q1/6841 , C12Q1/6874 , C12Q1/6876 , C12N15/10
摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.
-
公开(公告)号：US20210198659A1
公开(公告)日：2021-07-01
申请号：US17197836
申请日：2021-03-10
申请人： Illumina, Inc.
发明人： Neeraj Salathia , Jian-Bing Fan , Fiona Kaper , Gordon M. Cann , Arash Jamshidi , Alex Aravanis
IPC分类号： C12N15/10 , C12Q1/6869 , C12Q1/6853 , C12Q1/6806
摘要： Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).
-
公开(公告)号：US20240068016A1
公开(公告)日：2024-02-29
申请号：US18505624
申请日：2023-11-09
发明人： Jonas Frisen , Patrik Stahl , Joakim Lundeberg , Gordon M. Cann , Leila Bazargan , Alex Aravanis
IPC分类号： C12Q1/6834 , C12N15/10 , C12Q1/6841 , C12Q1/6874 , C12Q1/6876
CPC分类号： C12Q1/6834 , C12N15/1065 , C12Q1/6841 , C12Q1/6874 , C12Q1/6876
摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.
-
4.发明申请公开(公告)号：US20210032696A1
公开(公告)日：2021-02-04
申请号：US16947922
申请日：2020-08-24
申请人： Illumina, Inc.
IPC分类号： C12Q1/6874 , C12Q1/6869 , G16B25/00 , G16B30/00 , C12Q1/6827
摘要： Disclosed are methods for determining at least one sequence of interest of a fetus of a pregnant mother. In various embodiments, the method can determine one or more sequences of interest in a test sample that comprises a mixture of fetal cellular DNA and mother-and-fetus cfDNA. In some embodiments, methods are provided for determining whether the fetus has a genetic disease. In some embodiments, methods are provided for determining whether the fetus is homozygous in a disease causing allele when the mother is heterozygous of the same allele. In some embodiments, methods are provided for determining whether the fetus has a copy number variation (CNV) or a non-CNV genetic sequence anomaly.
-
5.发明申请NON-INVASIVE PRENATAL DIAGNOSIS OF FETAL GENETIC CONDITION USING CELLULAR DNA AND CELL FREE DNA 有权使用细胞DNA和细胞免费DNA进行基因遗传状态的非侵入性初步诊断公开(公告)号：US20160186253A1
公开(公告)日：2016-06-30
申请号：US14802873
申请日：2015-07-17
申请人： Illumina, Inc.
CPC分类号： C12Q1/6874 , C12Q1/6827 , C12Q1/6869 , G06F19/20 , G06F19/22 , C12Q2525/161 , C12Q2545/113 , C12Q2545/114 , C12Q2563/179
摘要： Disclosed are methods for determining at least one sequence of interest of a fetus of a pregnant mother. In various embodiments, the method can determine one or more sequences of interest in a test sample that comprises a mixture of fetal cellular DNA and mother-and-fetus cfDNA. In some embodiments, methods are provided for determining whether the fetus has a genetic disease. In some embodiments, methods are provided for determining whether the fetus is homozygous in a disease causing allele when the mother is heterozygous of the same allele. In some embodiments, methods are provided for determining whether the fetus has a copy number variation (CNV) or a non-CNV genetic sequence anomaly.
摘要翻译： 公开了用于确定怀孕母亲的胎儿的至少一个感兴趣序列的方法。 在各种实施方案中，该方法可以测定包含胎儿细胞DNA和母亲和胎儿cfDNA的混合物的测试样品中的一种或多种感兴趣的序列。 在一些实施例中，提供了用于确定胎儿是否具有遗传性疾病的方法。 在一些实施方案中，提供了用于在母体是相同等位基因杂合的情况下，确定胎儿在导致等位基因的疾病中是否是纯合的方法。 在一些实施例中，提供了用于确定胎儿是否具有拷贝数变异（CNV）或非CNV遗传序列异常的方法。
-
公开(公告)号：US20160017396A1
公开(公告)日：2016-01-21
申请号：US14804068
申请日：2015-07-20
申请人： ILLUMINA, INC.
发明人： Gordon M. Cann , Jeffrey G. Mandell , Alex Aravanis , Steven Norberg , Dmitry K. Pokholok , Frank J. Steemers , Farnaz Absalan , Leila Bazargan
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12N15/102 , C12Q1/6816 , C12Q1/683 , C12Q1/6869 , C12Q2521/301 , C12Q2522/101 , C12Q2563/131
摘要： A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.
摘要翻译： 一种富含靶核酸的方法，包括提供具有crRNA或其衍生物的内切核酸酶系统和Cas蛋白质或其变体。 所述crRNA或其衍生物含有与靶核酸区域基本上互补的靶特异性核苷酸区域; 使靶核酸与内切核酸酶系统接触以形成复合物; 并分离复合物，从而富集靶核酸。
-
7.发明授权公开(公告)号：US11519031B2
公开(公告)日：2022-12-06
申请号：US16947922
申请日：2020-08-24
申请人： Illumina, Inc.
IPC分类号： C12Q1/68 , C12Q1/6874 , C12Q1/6869 , G16B25/00 , G16B30/00 , C12Q1/6827 , G16B30/10
摘要： Disclosed are methods for determining at least one sequence of interest of a fetus of a pregnant mother. In various embodiments, the method can determine one or more sequences of interest in a test sample that comprises a mixture of fetal cellular DNA and mother-and-fetus cfDNA. In some embodiments, methods are provided for determining whether the fetus has a genetic disease. In some embodiments, methods are provided for determining whether the fetus is homozygous in a disease causing allele when the mother is heterozygous of the same allele. In some embodiments, methods are provided for determining whether the fetus has a copy number variation (CNV) or a non-CNV genetic sequence anomaly.
-
公开(公告)号：US11162132B2
公开(公告)日：2021-11-02
申请号：US17237670
申请日：2021-04-22
发明人： Jonas Frisen , Patrik Stahl , Joakim Lundeberg , Gordon M. Cann , Leila Bazargan , Alex Aravanis
IPC分类号： C12Q1/6834 , C12Q1/6876 , C12Q1/6841 , C12Q1/6874
摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.
-
9.发明申请公开(公告)号：US20180237852A1
公开(公告)日：2018-08-23
申请号：US15959093
申请日：2018-04-20
申请人： Illumina, Inc.
IPC分类号： C12Q1/6874 , G06F19/22 , C12Q1/6827 , G06F19/20 , C12Q1/6869
摘要： Disclosed are methods for determining at least one sequence of interest of a fetus of a pregnant mother. In various embodiments, the method can determine one or more sequences of interest in a test sample that comprises a mixture of fetal cellular DNA and mother-and-fetus cfDNA. In some embodiments, methods are provided for determining whether the fetus has a genetic disease. In some embodiments, methods are provided for determining whether the fetus is homozygous in a disease causing allele when the mother is heterozygous of the same allele. In some embodiments, methods are provided for determining whether the fetus has a copy number variation (CNV) or a non-CNV genetic sequence anomaly.
-
10.发明授权公开(公告)号：US09982295B2
公开(公告)日：2018-05-29
申请号：US14802873
申请日：2015-07-17
申请人： Illumina, Inc.
CPC分类号： C12Q1/6874 , C12Q1/6827 , C12Q1/6869 , G06F19/20 , G06F19/22 , C12Q2525/161 , C12Q2545/113 , C12Q2545/114 , C12Q2563/179
摘要： Disclosed are methods for determining at least one sequence of interest of a fetus of a pregnant mother. In various embodiments, the method can determine one or more sequences of interest in a test sample that comprises a mixture of fetal cellular DNA and mother-and-fetus cfDNA. In some embodiments, methods are provided for determining whether the fetus has a genetic disease. In some embodiments, methods are provided for determining whether the fetus is homozygous in a disease causing allele when the mother is heterozygous of the same allele. In some embodiments, methods are provided for determining whether the fetus has a copy number variation (CNV) or a non-CNV genetic sequence anomaly.
-
-
-
-
-
-
-
-
-
搜索结果
24 条记录 (耗时 0.023 秒)
